FastQCFastQC Report
Mon 6 Jun 2016
ERR1211513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345299
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252100.30208624040912135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218280.2615604306088973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137410.16465557435389672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131700.047.2918431
GTATCAA221800.036.0615231
TACCTGG76600.032.3493042
GTACCTG86400.029.9682311
TCAACGC261500.029.7384134
ATCAACG263500.029.6207523
CAACGCA264400.029.391825
GTACATG276550.029.2797241
AACGCAG275250.028.3303896
TATCAAC279550.028.1397082
TACATGG282800.028.1322252
ACATGGG282150.027.1132413
CATGGGG175050.026.4134084
ACGCAGA309700.025.028387
ACCTGGG96850.024.9385173
CGCAGAG314400.024.4315628
ATGGGGG115600.023.8579275
GCAGAGT352450.021.089399
GAGTACT210100.020.25128612-13
TGGGGGG119850.019.7152236
Produced by FastQC (version 0.11.5)