FastQCFastQC Report
Mon 6 Jun 2016
ERR1211513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8345299
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265400.3180233566226926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231170.27700625226250136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159670.19132927412187387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143800.044.344431
GTATCAA236950.035.8425831
TACCTGG78550.031.2503412
GTACCTG86950.028.9349331
TCAACGC287550.028.9266954
ATCAACG291350.028.4692753
CAACGCA292450.028.4254475
GTACATG297850.027.5824971
AACGCAG304900.027.3106616
TATCAAC307650.027.2383772
TACATGG301900.026.5266272
ACATGGG297600.026.2291033
CATGGGG177150.025.6787054
ACCTGGG95400.024.8765263
ACGCAGA348700.023.7723737
ATGGGGG115700.023.2323845
CGCAGAG356050.023.1232598
GCAGAGT397100.020.4723139
TGGGGGG105550.020.07916
GAGTACT226650.019.6658512-13
Produced by FastQC (version 0.11.5)