##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8345299 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08661906541635 33.0 31.0 34.0 30.0 34.0 2 32.30969399658419 34.0 31.0 34.0 30.0 34.0 3 32.32421498618564 34.0 31.0 34.0 30.0 34.0 4 35.97282697719998 37.0 35.0 37.0 35.0 37.0 5 35.885599305668975 37.0 35.0 37.0 35.0 37.0 6 35.90147387169711 37.0 35.0 37.0 35.0 37.0 7 35.82393380992101 37.0 35.0 37.0 35.0 37.0 8 35.81570582432097 37.0 35.0 37.0 35.0 37.0 9 37.46559242514858 39.0 37.0 39.0 34.0 39.0 10-11 37.421474473233374 39.0 37.0 39.0 34.0 39.0 12-13 37.33207504009143 39.0 37.0 39.0 34.0 39.0 14-15 38.557515794221395 40.0 38.0 41.0 34.0 41.0 16-17 38.48104489725293 40.0 38.0 41.0 34.0 41.0 18-19 38.460683014473176 40.0 38.0 41.0 34.0 41.0 20-21 38.40006505458942 40.0 38.0 41.0 34.0 41.0 22-23 38.297211639750714 40.0 38.0 41.0 34.0 41.0 24-25 38.1885128381859 40.0 38.0 41.0 33.0 41.0 26-27 38.011855357129804 40.0 37.0 41.0 33.0 41.0 28-29 38.0502990366193 40.0 37.5 41.0 33.0 41.0 30-31 37.97867763635551 40.0 37.0 41.0 33.0 41.0 32-33 37.8053463992123 40.0 37.0 41.0 33.0 41.0 34-35 37.697586629310706 40.0 37.0 41.0 33.0 41.0 36-37 37.648450462949256 40.0 37.0 41.0 32.5 41.0 38-39 37.55664955803262 40.0 37.0 41.0 32.0 41.0 40-41 37.40143870219629 40.0 36.5 41.0 31.5 41.0 42-43 37.25341979957818 40.0 36.0 41.0 31.0 41.0 44-45 37.08108085761816 39.0 36.0 41.0 31.0 41.0 46-47 36.83774979182891 39.0 36.0 41.0 30.0 41.0 48-49 36.62710269578118 39.0 35.0 41.0 30.0 41.0 50-51 36.433104673661184 39.0 35.0 40.5 29.5 41.0 52-53 36.235082829267114 39.0 35.0 40.0 28.5 41.0 54-55 36.04191191951301 39.0 35.0 40.0 28.0 41.0 56-57 35.839232482862506 38.0 34.5 40.0 28.0 41.0 58-59 35.57756648383719 38.0 34.0 40.0 27.5 41.0 60-61 35.26423738682101 38.0 34.0 40.0 26.5 41.0 62-63 34.932844227630426 37.0 34.0 40.0 26.0 41.0 64-65 34.61362331056083 37.0 33.0 40.0 26.0 41.0 66-67 34.27054980294894 36.0 33.0 39.0 26.0 41.0 68-69 33.87013077662047 36.0 32.0 39.0 25.0 40.0 70-71 33.479638836187895 35.0 32.0 38.5 24.5 40.0 72-73 33.06564558082341 35.0 31.5 38.0 24.0 40.0 74-75 32.651255874714614 35.0 31.0 37.0 23.0 39.0 76-77 31.11159462351199 33.5 29.5 35.5 21.5 38.5 78-79 31.945662462183797 34.0 31.0 36.0 22.5 39.0 80-81 31.913078249203537 34.0 31.0 36.0 22.5 37.5 82-83 31.62221113946906 34.0 31.0 35.5 22.0 37.0 84-85 31.320019210815573 34.0 30.0 35.0 20.5 37.0 86-87 31.018911784946233 34.0 30.0 35.0 20.0 36.0 88-89 30.739249965759164 34.0 30.0 35.0 20.0 36.0 90-91 30.451691724886068 34.0 29.0 35.0 19.0 35.5 92-93 30.23371415451981 34.0 29.0 35.0 19.0 35.0 94-95 30.010612921118824 34.0 29.0 35.0 18.0 35.0 96-97 29.801149665218706 34.0 29.0 35.0 18.0 35.0 98-99 29.634234615200725 33.5 29.0 35.0 17.5 35.0 100 27.74418184417359 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3130771566285375 1101 2 0.24696975459769988 1101 3 0.3056270039835596 1101 4 0.1614038399876776 1101 5 0.22326594764680152 1101 6 0.18681988674158134 1101 7 0.2229927327801704 1101 8 0.20560698791648235 1101 9 0.26292641926008997 1101 10-11 0.2747284125885372 1101 12-13 0.26521494029330483 1101 14-15 0.37078528073116956 1101 16-17 0.41007701750763204 1101 18-19 0.4093570601294445 1101 20-21 0.33734786861165134 1101 22-23 0.31055342934273966 1101 24-25 0.292230558533241 1101 26-27 0.29216411196080116 1101 28-29 0.33546756738690675 1101 30-31 0.4109140877704718 1101 32-33 0.4485666760711027 1101 34-35 0.3837714672292094 1101 36-37 0.4292235637055981 1101 38-39 0.4230541022106422 1101 40-41 0.4158367821172888 1101 42-43 0.4569244337142919 1101 44-45 0.41719143853572405 1101 46-47 0.5282686116554132 1101 48-49 0.4199386657540245 1101 50-51 0.4267077581499237 1101 52-53 0.4123634270561851 1101 54-55 0.41846859416688886 1101 56-57 0.42858357876677644 1101 58-59 0.3999384454760886 1101 60-61 0.45090720193901745 1101 62-63 0.3745234379795761 1101 64-65 0.36589406212322473 1101 66-67 0.3183742827124689 1101 68-69 0.2990313238835256 1101 70-71 0.31644987571157657 1101 72-73 0.18668657827446822 1101 74-75 0.2991416957350239 1101 76-77 0.28459227682078136 1101 78-79 0.3795060215484085 1101 80-81 0.3697996836664039 1101 82-83 0.35057241006936124 1101 84-85 0.2899846774792074 1101 86-87 0.2546306109293006 1101 88-89 0.2829112800797944 1101 90-91 0.1366425438270511 1101 92-93 0.14053247205979247 1101 94-95 0.2398386619605084 1101 96-97 0.11562635894129869 1101 98-99 -0.0018237695454956793 1101 100 0.22557744815251723 1102 1 0.2938257023425095 1102 2 0.21785480896863874 1102 3 0.32555847897553747 1102 4 0.21808640692351844 1102 5 0.19162830430474997 1102 6 0.17776637794135297 1102 7 0.1764690574118859 1102 8 0.15743680838790652 1102 9 0.24445943054715968 1102 10-11 0.2371524343850453 1102 12-13 0.2675909778264227 1102 14-15 0.366182480531954 1102 16-17 0.3358941057599196 1102 18-19 0.2854977842563571 1102 20-21 0.35670193154135177 1102 22-23 0.3134720441741834 1102 24-25 0.30977248281343606 1102 26-27 0.3393622518248094 1102 28-29 0.34920959058548107 1102 30-31 0.3936164762931327 1102 32-33 0.425147832365397 1102 34-35 0.41374149875461086 1102 36-37 0.4879688157456954 1102 38-39 0.5111748653403794 1102 40-41 0.4587654706252664 1102 42-43 0.3991535448448573 1102 44-45 0.3927932162139811 1102 46-47 0.46346543252860073 1102 48-49 0.40621253272242086 1102 50-51 0.4259572003153309 1102 52-53 0.4448124434792149 1102 54-55 0.44512248927526343 1102 56-57 0.42815895357178135 1102 58-59 0.5615999531867075 1102 60-61 0.5760603438689316 1102 62-63 0.5874033750511884 1102 64-65 0.5043577325604502 1102 66-67 0.5336759905726467 1102 68-69 0.5673406855866929 1102 70-71 0.5452905703697795 1102 72-73 0.5359730034070296 1102 74-75 0.5371172872738228 1102 76-77 0.5085941779360503 1102 78-79 0.48007318879099614 1102 80-81 0.4276669594125586 1102 82-83 0.4414941271082711 1102 84-85 0.4096855548909666 1102 86-87 0.3611834151010598 1102 88-89 0.37139093279228774 1102 90-91 0.37670300965615056 1102 92-93 0.33039524674085285 1102 94-95 0.3808238237891466 1102 96-97 0.44385554363964985 1102 98-99 0.32110851059184853 1102 100 0.30851406510506507 1103 1 0.2963190626197161 1103 2 0.30426061560805095 1103 3 0.31414611398400183 1103 4 0.13760934351824972 1103 5 0.1531492739073954 1103 6 0.17797222183241956 1103 7 0.21997440462487106 1103 8 0.20646094648832758 1103 9 0.25648631676269673 1103 10-11 0.22311793272470481 1103 12-13 0.24802401429759158 1103 14-15 0.36513486191945077 1103 16-17 0.33614889601324904 1103 18-19 0.36716505531497035 1103 20-21 0.3204695816721852 1103 22-23 0.3345885213224449 1103 24-25 0.3172480656797916 1103 26-27 0.3503806198503838 1103 28-29 0.37692160775679895 1103 30-31 0.4332384148743742 1103 32-33 0.47372904484612377 1103 34-35 0.49724586987372277 1103 36-37 0.503401901269477 1103 38-39 0.4413735454248595 1103 40-41 0.5121246555712986 1103 42-43 0.5000485903502749 1103 44-45 0.49962621338639224 1103 46-47 0.5380793982007717 1103 48-49 0.5138430864300929 1103 50-51 0.5235582654335076 1103 52-53 0.4783178753389521 1103 54-55 0.5221663655806523 1103 56-57 0.5947053793557657 1103 58-59 0.6894997019700355 1103 60-61 0.6637570311245895 1103 62-63 0.6588329406869278 1103 64-65 0.6306277126904192 1103 66-67 0.6338193800388154 1103 68-69 0.6263914080785611 1103 70-71 0.6474992696542046 1103 72-73 0.5415941495672101 1103 74-75 0.5537025425399023 1103 76-77 0.6262142343844488 1103 78-79 0.5861174305649115 1103 80-81 0.5373952118301553 1103 82-83 0.48398169457288276 1103 84-85 0.4684511347181797 1103 86-87 0.5564590180063718 1103 88-89 0.6097028195084491 1103 90-91 0.5452587154862449 1103 92-93 0.4774777371926824 1103 94-95 0.5402035210859957 1103 96-97 0.6454749067638907 1103 98-99 0.6048915147219383 1103 100 0.6122809268523817 1104 1 0.2760210347510892 1104 2 0.2417861226249869 1104 3 0.2697513042621438 1104 4 0.022591483078912233 1104 5 0.1195526972549601 1104 6 0.14280892348666185 1104 7 0.17014275289459135 1104 8 0.1665422985625611 1104 9 0.20914806461076552 1104 10-11 0.2233933371171517 1104 12-13 0.2848108093070181 1104 14-15 0.3878481213655647 1104 16-17 0.33046244053429064 1104 18-19 0.36332381047024853 1104 20-21 0.27575070157630677 1104 22-23 0.3522827444655974 1104 24-25 0.3784983302912721 1104 26-27 0.4109834726793835 1104 28-29 0.42914197720160274 1104 30-31 0.41208817204871906 1104 32-33 0.34078412604993247 1104 34-35 0.4559659540761132 1104 36-37 0.3762565059322256 1104 38-39 0.4262985437250393 1104 40-41 0.5295855494910313 1104 42-43 0.5405977633605445 1104 44-45 0.49039993730838916 1104 46-47 0.4819738411445087 1104 48-49 0.4981030331862044 1104 50-51 0.5277168332024615 1104 52-53 0.5247011705618476 1104 54-55 0.5233423918578239 1104 56-57 0.6103702768531392 1104 58-59 0.6054174589432577 1104 60-61 0.5697883463523254 1104 62-63 0.5934398864948704 1104 64-65 0.6172004382284157 1104 66-67 0.6908823580481851 1104 68-69 0.7063192101248674 1104 70-71 0.5841336433876663 1104 72-73 0.6990244430792316 1104 74-75 0.7171272282705061 1104 76-77 0.5525917193301346 1104 78-79 0.642590085036602 1104 80-81 0.8118624136153763 1104 82-83 0.6870845418894618 1104 84-85 0.6398540171251064 1104 86-87 0.6897686665860547 1104 88-89 0.7384471676607589 1104 90-91 0.6017852591885919 1104 92-93 0.6622522276008773 1104 94-95 0.677005420743729 1104 96-97 0.5913946871286164 1104 98-99 0.6692436060982168 1104 100 0.832745751679731 1105 1 0.21881594290822193 1105 2 0.12747198574674456 1105 3 0.1563286576195182 1105 4 0.13429554580837788 1105 5 0.07874227451501525 1105 6 0.11159839085507883 1105 7 0.1586637369853321 1105 8 0.11791768786062562 1105 9 0.16426808481963207 1105 10-11 0.17627915759850765 1105 12-13 0.20349078897992 1105 14-15 0.28106033990864177 1105 16-17 0.2508179930323706 1105 18-19 0.25236765421841056 1105 20-21 0.23673473714124071 1105 22-23 0.26890601827084737 1105 24-25 0.3014038584100476 1105 26-27 0.29838236552656383 1105 28-29 0.40495246742923285 1105 30-31 0.38081250394274235 1105 32-33 0.43096517557516023 1105 34-35 0.4127153463666957 1105 36-37 0.44339515170943855 1105 38-39 0.3940908127193552 1105 40-41 0.4343389206490045 1105 42-43 0.36340120033625567 1105 44-45 0.3748774505573991 1105 46-47 0.45070383190608965 1105 48-49 0.4865106675120856 1105 50-51 0.559390567850123 1105 52-53 0.5260019773402504 1105 54-55 0.5319909025621143 1105 56-57 0.5298938341953701 1105 58-59 0.5395494598429664 1105 60-61 0.5601253343165595 1105 62-63 0.5460374372433989 1105 64-65 0.5593443980455604 1105 66-67 0.4748471089314137 1105 68-69 0.596071015765439 1105 70-71 0.5938945803099216 1105 72-73 0.5638259075916849 1105 74-75 0.6405348377195352 1105 76-77 0.6106887386007784 1105 78-79 0.6729753035474602 1105 80-81 0.6748445245003118 1105 82-83 0.6980141242470523 1105 84-85 0.7068842207734001 1105 86-87 0.7281652036201969 1105 88-89 0.7207230957175028 1105 90-91 0.71099420467716 1105 92-93 0.7118982300290533 1105 94-95 0.777701988546351 1105 96-97 0.7495362113297546 1105 98-99 0.795438501536136 1105 100 0.9659980308178859 1106 1 0.21317630833064527 1106 2 0.19789848389136466 1106 3 0.22904574369701436 1106 4 0.16767697009113647 1106 5 0.1369510238751559 1106 6 0.09828801608722415 1106 7 0.1320818177482792 1106 8 0.11975298266907686 1106 9 0.10377200558973954 1106 10-11 0.1562563007718012 1106 12-13 0.2201936673830005 1106 14-15 0.2453406921457173 1106 16-17 0.25525454844236606 1106 18-19 0.30287902963070934 1106 20-21 0.26895976703063695 1106 22-23 0.25719671506420383 1106 24-25 0.32362350365372805 1106 26-27 0.28112639154689134 1106 28-29 0.3105607349739259 1106 30-31 0.30168589934060464 1106 32-33 0.3435944177307917 1106 34-35 0.3615520676496402 1106 36-37 0.3416280303576613 1106 38-39 0.3808757029902097 1106 40-41 0.39415710110625923 1106 42-43 0.29556268830554444 1106 44-45 0.32316153651058954 1106 46-47 0.43107805303521474 1106 48-49 0.4654708953061686 1106 50-51 0.421584514606252 1106 52-53 0.4494578843370576 1106 54-55 0.399040986331876 1106 56-57 0.3927172593348658 1106 58-59 0.5000566843132859 1106 60-61 0.4847260205300614 1106 62-63 0.4699673984789854 1106 64-65 0.5396953706536962 1106 66-67 0.5878190242335961 1106 68-69 0.5420405264474724 1106 70-71 0.5839301394647691 1106 72-73 0.5256237731897286 1106 74-75 0.48540288325664704 1106 76-77 0.5195829202475117 1106 78-79 0.6592526505665006 1106 80-81 0.6346121504764106 1106 82-83 0.601825370141043 1106 84-85 0.6185676980767916 1106 86-87 0.4714750374769068 1106 88-89 0.5619227680895804 1106 90-91 0.5648358886321496 1106 92-93 0.6543983213517137 1106 94-95 0.7571018065695974 1106 96-97 0.7285165991463316 1106 98-99 0.6922934859463616 1106 100 0.9217204928690812 1107 1 0.2161140803777215 1107 2 0.20085861700252394 1107 3 0.2351318645070819 1107 4 0.12139244381990721 1107 5 0.12516473325820243 1107 6 0.1369911427296273 1107 7 0.17787579667193398 1107 8 0.1717841579880215 1107 9 0.23917999475744978 1107 10-11 0.18711050738018997 1107 12-13 0.21027817222708478 1107 14-15 0.2376204866328564 1107 16-17 0.23541089127373027 1107 18-19 0.21405813311282884 1107 20-21 0.27074700335933954 1107 22-23 0.3450248850417026 1107 24-25 0.3065877426317414 1107 26-27 0.306749047892545 1107 28-29 0.3093028107868818 1107 30-31 0.23211689212202202 1107 32-33 0.24727057786864037 1107 34-35 0.2801085290596319 1107 36-37 0.3555240416372598 1107 38-39 0.3427920187418181 1107 40-41 0.32284243252984623 1107 42-43 0.36702728312294397 1107 44-45 0.3648626764843712 1107 46-47 0.2653911431708309 1107 48-49 0.3338809555320452 1107 50-51 0.27007615612955505 1107 52-53 0.27781805122810255 1107 54-55 0.27322919124848255 1107 56-57 0.3748353763484076 1107 58-59 0.44126687737688286 1107 60-61 0.4255806149067354 1107 62-63 0.4172946904898609 1107 64-65 0.5362831240260348 1107 66-67 0.4864188557577762 1107 68-69 0.46135979138995964 1107 70-71 0.5442754279921829 1107 72-73 0.4566776082578414 1107 74-75 0.46701678715928807 1107 76-77 0.4530189006534435 1107 78-79 0.6056363790869099 1107 80-81 0.5290611282448161 1107 82-83 0.5381392844828596 1107 84-85 0.49298087359123954 1107 86-87 0.40204458340697613 1107 88-89 0.4276643999788554 1107 90-91 0.3612988400677928 1107 92-93 0.3795686583820519 1107 94-95 0.63270523938046 1107 96-97 0.6723402265302241 1107 98-99 0.6384944205907033 1107 100 0.6557546991351906 1108 1 0.2581983092153095 1108 2 0.205763054339684 1108 3 0.23442871988196856 1108 4 0.18865502591405914 1108 5 0.13595907397176177 1108 6 0.14765697342539852 1108 7 0.1649927925832202 1108 8 0.13545297615419116 1108 9 0.16489488508849348 1108 10-11 0.1777430594899343 1108 12-13 0.2095892402519226 1108 14-15 0.2693500607298134 1108 16-17 0.30819250050531366 1108 18-19 0.35825434981033055 1108 20-21 0.3128963695347622 1108 22-23 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86-87 -0.20866922111249409 2316 88-89 -0.19249431396622896 2316 90-91 -0.0334222037242391 2316 92-93 0.03214599289715281 2316 94-95 0.09295157032941148 2316 96-97 0.08280660305164034 2316 98-99 -0.09386733013546333 2316 100 -0.4045028813852234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 18.0 11 43.0 12 109.0 13 277.0 14 828.0 15 2110.0 16 4622.0 17 9256.0 18 16602.0 19 25631.0 20 36257.0 21 50066.0 22 64879.0 23 76280.0 24 83685.0 25 94663.0 26 110760.0 27 133453.0 28 161114.0 29 193925.0 30 233559.0 31 281446.0 32 343108.0 33 427675.0 34 558684.0 35 746080.0 36 1086392.0 37 1676991.0 38 1664141.0 39 262609.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92775626396939 20.219249114562587 13.672009249100183 24.180985372367832 2 14.891172697386368 21.551701706988908 39.88921521005428 23.667910385570444 3 17.062639518373796 27.24272209731383 31.009102568450203 24.68553581586217 4 11.914412975928665 17.62665338324124 38.254635548397246 32.20429809243284 5 12.297472281518113 38.55755088522033 35.536156672483216 13.60882016077834 6 30.38075021254644 38.11680557827311 18.245969106921496 13.256475102258955 7 26.549078708863515 33.13895643523377 23.000733706485534 17.311231149417175 8 25.546634705947486 36.582273687863406 20.464913907849787 17.406177698339317 9 26.65342807410832 15.152640444954752 20.371651198075853 37.82228028286108 10-11 24.333334252014218 26.579778627464396 29.450316878999782 19.636570241521603 12-13 24.83271120663262 24.364441585616046 29.248532616985923 21.55431459076541 14-15 22.29053746306753 25.796631133288333 26.65081263115917 25.26201877248496 16-17 21.897466957666833 28.638572053307264 27.389351164972275 22.074609824053624 18-19 21.774316179990567 27.860145005251514 29.09126966770778 21.274269147050138 20-21 22.500938433096835 27.198724030567906 28.990467676709276 21.309869859625987 22-23 22.24893919319128 27.002567553301564 28.872488571110516 21.87600468239664 24-25 22.03019521416616 27.091267943042403 28.904427116785314 21.974109726006123 26-27 21.940335904565057 27.22083734208302 28.90986253678274 21.928964216569184 28-29 22.015310904908116 27.398680575373103 28.936271786925904 21.649736732792878 30-31 22.460281853784306 27.173938051411245 28.914368273510764 21.451411821293686 32-33 22.22541905965127 27.27325398652351 28.83776771699353 21.663559236831688 34-35 22.29487416540972 27.443339173582913 28.826101445866207 21.435685215141156 36-37 22.272169139767048 27.39017539798061 28.72056404207788 21.617091420174464 38-39 22.249907521500727 27.520858440132468 28.686009641729846 21.54322439663696 40-41 22.29878377046435 27.290109256040928 28.781089169095058 21.630017804399664 42-43 22.09154271996794 27.43449267776159 28.56554974429327 21.908414857977192 44-45 22.009374518228626 27.59048973346513 28.607367736924864 21.792768011381384 46-47 22.032423608599544 27.436420290574087 28.36734405292351 22.16381204790285 48-49 22.05552251347851 27.527356383108128 28.40095947321009 22.016161630203275 50-51 22.068870266738287 27.703806142952885 28.30710630015031 21.92021729015852 52-53 22.1311920362115 27.726470774680756 28.12689747468326 22.01543971442448 54-55 22.0240495418453 27.63338444911491 28.35248847018978 21.990077538850013 56-57 22.136656963359005 27.703437679091035 28.00030077557559 22.159604581974378 58-59 22.065066318739415 27.680935482690046 28.239746498503692 22.014251700066847 60-61 22.298242835708294 27.7452789754989 28.18769330086469 21.768784887928117 62-63 22.186743613176247 27.774112203020284 28.17846727734989 21.86067690645358 64-65 22.04617711031944 27.58228113098725 28.172272187861484 22.19926957083182 66-67 22.012480520720164 27.601798729897325 28.059516491423302 22.326204257959205 68-69 21.843536406493225 27.66192644602576 28.247586037215733 22.246951110265286 70-71 21.911519203665883 27.485197737188223 28.22866324922506 22.37461980992084 72-73 21.855739744506113 27.824747594614834 27.91861599179308 22.40089666908598 74-75 21.89821152285235 27.813848683301934 27.82604751637211 22.461892277473602 76-77 21.776494598187266 27.64074209572144 28.006766630689334 22.575996675401967 78-79 21.707616784171464 27.577899255856224 28.06505721528016 22.649426744692153 80-81 21.76390133256911 27.829861648911443 27.78517524732857 22.621061771190885 82-83 21.870025967289628 27.757782606554038 27.864434616708927 22.50775680944741 84-85 21.791520501828284 27.844716313374217 27.718090722672518 22.64567246212498 86-87 21.620961287181615 27.809009906947228 27.8599001994142 22.710128606456955 88-89 21.527103355509716 27.613707144946865 27.929849137556495 22.929340361986927 90-91 21.834066473945832 27.588142574744445 27.85407958861505 22.723711362694676 92-93 21.672416038562993 27.60421314675789 27.837290607719705 22.88608020695941 94-95 21.48799531921109 27.51657884293722 27.804213369317516 23.191212468534168 96-97 21.402023581200336 27.471322931371354 27.922906010073078 23.203747477355225 98-99 21.349257917853002 27.421111506148915 27.79032037792434 23.43931019807374 100 20.435940621909737 28.076089518970544 27.9430438420322 23.54492601708752 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2796.0 1 2057.5 2 2302.5 3 3218.5 4 2476.0 5 2122.5 6 3281.0 7 5994.5 8 9380.5 9 11008.5 10 9532.5 11 7703.0 12 7574.5 13 7997.0 14 8595.0 15 9221.5 16 9785.5 17 10364.5 18 11173.0 19 12338.0 20 13774.0 21 15740.5 22 18751.5 23 23174.5 24 29038.0 25 36436.5 26 46520.0 27 59083.0 28 72611.5 29 87541.0 30 104466.0 31 120407.5 32 137888.5 33 157952.5 34 177418.5 35 195065.0 36 216457.5 37 237097.0 38 251008.0 39 262725.5 40 268976.5 41 274498.5 42 282510.5 43 290467.5 44 298283.5 45 307165.0 46 314919.5 47 319759.0 48 325325.5 49 329006.0 50 327634.0 51 317024.5 52 299824.0 53 284001.5 54 266043.5 55 243331.0 56 218715.0 57 193283.5 58 168712.0 59 142178.5 60 113642.0 61 89888.0 62 71291.5 63 54545.5 64 39681.5 65 28742.5 66 20899.5 67 15395.5 68 11322.5 69 8032.5 70 5708.0 71 4068.5 72 2950.0 73 2116.0 74 1551.0 75 1071.5 76 676.0 77 437.0 78 263.0 79 172.0 80 115.0 81 90.0 82 87.0 83 62.0 84 35.5 85 26.5 86 22.0 87 14.0 88 10.5 89 9.0 90 7.5 91 5.5 92 2.0 93 1.0 94 0.0 95 2.0 96 3.5 97 2.5 98 1.5 99 2.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003247337213441963 2 0.06621692044826674 3 8.627611784790454E-4 4 7.429332370236225E-4 5 0.0017375051511036334 6 7.668988253147071E-4 7 0.0 8 6.590536780048265E-4 9 1.1982794145542299E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.890106633686821E-4 18-19 0.001006554708225553 20-21 0.003253328610514734 22-23 0.0 24-25 3.59483824366269E-5 26-27 1.437935297465076E-4 28-29 8.447869872607321E-4 30-31 0.0014499180916106183 32-33 0.0029477673598034054 34-35 0.004026218832902212 36-37 0.0012701761794274838 38-39 0.0013900041208829066 40-41 0.005410231556712348 42-43 0.006985968986851159 44-45 0.005314369203548009 46-47 0.006914072221977906 48-49 0.008627611784790455 50-51 0.008346016122370212 52-53 0.00390639089144679 54-55 0.0023006964759441214 56-57 0.002474446991054485 58-59 0.003109535080768227 60-61 0.007579117297055504 62-63 0.01375025628200979 64-65 0.0040981155977754665 66-67 0.010251280391511436 68-69 0.012366243558199652 70-71 0.005512085306949457 72-73 0.007639031267783216 74-75 0.0028578964037118382 76-77 0.0027560426534747285 78-79 0.008088386048241053 80-81 0.00573376699864199 82-83 0.016056944155026683 84-85 0.016883756951069098 86-87 0.02229997990485422 88-89 0.020826096224952514 90-91 0.02360610446671833 92-93 0.033473935445572416 94-95 0.037332395160437036 96-97 0.022443773434600724 98-99 0.018123976145132725 100 0.018549365337299478 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8345299.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.443194482519225 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6934390610741 45.89403502797775 2 15.526365526821209 19.07978993342315 3 4.90318992477667 9.03802956398345 4 1.9094809501902323 4.6929843753281615 5 0.963477005697669 2.959955252025858 6 0.5471434219456685 2.017094381066327 7 0.34121351905449115 1.4675674027930914 8 0.2172298384000239 1.0677836166575112 9 0.16440648552985462 0.9091493698138504 >10 0.6806630145103661 7.052102553814602 >50 0.03293505966706363 1.380770040477022 >100 0.018289634426524485 2.102847793064252 >500 0.001611722024910479 0.6704403777375544 >1k 4.7557361263728977E-4 0.6021262673878942 >5k 0.0 0.0 >10k+ 7.926226862227521E-5 1.0653240444492844 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26540 0.3180233566226926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23117 0.27700625226250136 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15967 0.19132927412187387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1982794145542299E-5 0.0 4 0.0 0.0 0.0 1.1982794145542299E-5 0.0 5 0.0 0.0 0.0 1.1982794145542299E-5 0.0 6 3.59483824366269E-5 0.0 0.0 1.1982794145542299E-5 0.0 7 3.59483824366269E-5 1.1982794145542299E-5 0.0 1.1982794145542299E-5 0.0 8 4.7931176582169194E-5 1.1982794145542299E-5 0.0 2.3965588291084597E-5 0.0 9 4.7931176582169194E-5 1.1982794145542299E-5 0.0 2.3965588291084597E-5 0.0 10-11 4.7931176582169194E-5 1.1982794145542299E-5 0.0 2.3965588291084597E-5 0.0 12-13 4.7931176582169194E-5 1.1982794145542299E-5 0.0 2.3965588291084597E-5 0.0 14-15 4.7931176582169194E-5 2.9956985363855746E-5 0.0 2.3965588291084597E-5 1.1982794145542299E-5 16-17 8.387955901879608E-5 4.7931176582169194E-5 0.0 3.59483824366269E-5 2.3965588291084597E-5 18-19 8.387955901879608E-5 5.991397072771149E-5 0.0 6.590536780048264E-5 3.59483824366269E-5 20-21 8.387955901879608E-5 5.991397072771149E-5 0.0 8.38795590187961E-5 3.59483824366269E-5 22-23 8.387955901879608E-5 5.991397072771149E-5 0.0 1.3780213267373644E-4 5.991397072771149E-5 24-25 8.387955901879608E-5 8.38795590187961E-5 0.0 2.1569029461976138E-4 8.387955901879608E-5 26-27 8.387955901879608E-5 9.586235316433839E-5 0.0 4.01423603875667E-4 8.387955901879608E-5 28-29 8.387955901879608E-5 1.0185375023710954E-4 0.0 0.0012761675765002548 8.387955901879608E-5 30-31 8.387955901879608E-5 1.0784514730988069E-4 0.0 0.005062730526491621 8.987095609156724E-5 32-33 8.387955901879608E-5 1.0784514730988069E-4 0.0 0.014307456209777504 1.0784514730988069E-4 34-35 1.1982794145542298E-4 1.0784514730988069E-4 0.0 0.02799779852105958 1.3181073560096529E-4 36-37 1.1982794145542298E-4 1.1982794145542298E-4 0.0 0.051693773943869474 1.3181073560096529E-4 38-39 1.1982794145542298E-4 1.4978492681927874E-4 0.0 0.08766612196878745 1.3181073560096529E-4 40-41 1.1982794145542298E-4 1.7375051511036332E-4 0.0 0.1307502583190848 1.3181073560096529E-4 42-43 1.3181073560096529E-4 1.7974191218313447E-4 0.0 0.17339103128599706 1.3780213267373644E-4 44-45 1.3780213267373644E-4 1.7974191218313447E-4 0.0 0.22624114486491137 1.557763238920499E-4 46-47 1.437935297465076E-4 1.7974191218313447E-4 0.0 0.2901393946460157 1.6775911803759217E-4 48-49 1.437935297465076E-4 1.7974191218313447E-4 0.0 0.3590883921594661 1.7974191218313447E-4 50-51 1.7974191218313447E-4 1.7974191218313447E-4 0.0 0.43471779740905625 1.7974191218313447E-4 52-53 1.9172470632867678E-4 1.7974191218313447E-4 0.0 0.5156555804651217 1.8573330925590563E-4 54-55 1.9172470632867678E-4 1.7974191218313447E-4 0.0 0.6101698692880866 2.0969889754699023E-4 56-57 1.9172470632867678E-4 1.7974191218313447E-4 0.0 0.7167448404185398 2.276730887653037E-4 58-59 1.9771610340144793E-4 1.7974191218313447E-4 0.0 0.8423664628433325 2.276730887653037E-4 60-61 2.6362147120193057E-4 1.7974191218313447E-4 0.0 0.9725655126317223 2.3965588291084596E-4 62-63 2.875870594930152E-4 1.7974191218313447E-4 0.0 1.1091394089055406 2.6362147120193057E-4 64-65 2.875870594930152E-4 1.9172470632867678E-4 0.0 1.2493980143791132 2.6362147120193057E-4 66-67 2.9357845656578633E-4 1.9172470632867678E-4 0.0 1.4132687157164772 2.6362147120193057E-4 68-69 2.995698536385575E-4 1.9172470632867678E-4 0.0 1.6056105359436492 2.6362147120193057E-4 70-71 3.115526477840998E-4 1.9172470632867678E-4 0.0 1.8167293945968863 2.756042653474729E-4 72-73 3.5948382436626895E-4 2.0370750047421908E-4 0.0 2.0243013461830426 3.115526477840998E-4 74-75 3.7146661851181125E-4 2.0370750047421908E-4 0.0 2.239098922638961 3.115526477840998E-4 76-77 3.7146661851181125E-4 2.0370750047421908E-4 0.0 2.4788267023146804 3.1754404485687094E-4 78-79 3.7146661851181125E-4 2.0370750047421908E-4 0.0 2.7507462584623994 3.2952683900241324E-4 80-81 3.774580155845824E-4 2.0370750047421908E-4 0.0 3.0409934982557245 3.3551823607518434E-4 82-83 3.9543220680289586E-4 2.0370750047421908E-4 0.0 3.343906551460888 3.3551823607518434E-4 84-85 3.9543220680289586E-4 2.0370750047421908E-4 0.0 3.6570469194692725 3.3551823607518434E-4 86-87 4.01423603875667E-4 2.0370750047421908E-4 0.0 3.978113905804933 3.3551823607518434E-4 88 4.0741500094843816E-4 2.0370750047421908E-4 0.0 4.238901446191442 3.4750103022072664E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14380 0.0 44.34443 1 GTATCAA 23695 0.0 35.842583 1 TACCTGG 7855 0.0 31.250341 2 GTACCTG 8695 0.0 28.934933 1 TCAACGC 28755 0.0 28.926695 4 ATCAACG 29135 0.0 28.469275 3 CAACGCA 29245 0.0 28.425447 5 GTACATG 29785 0.0 27.582497 1 AACGCAG 30490 0.0 27.310661 6 TATCAAC 30765 0.0 27.238377 2 TACATGG 30190 0.0 26.526627 2 ACATGGG 29760 0.0 26.229103 3 CATGGGG 17715 0.0 25.678705 4 ACCTGGG 9540 0.0 24.876526 3 ACGCAGA 34870 0.0 23.772373 7 ATGGGGG 11570 0.0 23.232384 5 CGCAGAG 35605 0.0 23.123259 8 GCAGAGT 39710 0.0 20.472313 9 TGGGGGG 10555 0.0 20.0791 6 GAGTACT 22665 0.0 19.66585 12-13 >>END_MODULE