FastQCFastQC Report
Mon 6 Jun 2016
ERR1211512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12128884
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324870.26784822082559284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267370.22044072645100737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178850.14745791945903677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166300.047.7091561
GTATCAA289050.038.2977941
TCAACGC338350.031.5670834
CAACGCA343900.031.0684835
ATCAACG344450.031.0222033
AACGCAG358900.029.8820296
TATCAAC374900.028.7600122
GTACATG375900.027.8584251
TACATGG388000.026.0802042
ACGCAGA409500.025.9271897
ACATGGG382450.025.4948123
CGCAGAG416800.025.1584288
TACCTGG90650.023.394252
CATGGGG223050.023.0681674
GTACCTG102550.021.8098181
GCAGAGT476950.021.2683819
GAGTACT286600.019.41120712-13
ATGGGGG142200.019.395475
AGAGTAC433750.018.7881910-11
CAGAGTA470450.018.52595510-11
Produced by FastQC (version 0.11.5)