FastQCFastQC Report
Mon 6 Jun 2016
ERR1211512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12128884
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343770.2834308581069784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292210.24092076402082832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211170.174105053688369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167150.048.2215161
GTATCAA291150.037.439761
TCAACGC357700.029.6895434
ATCAACG360950.029.331453
CAACGCA362700.029.241395
AACGCAG380650.027.997816
TATCAAC386550.027.723852
GTACATG374650.026.999211
TACATGG389350.025.4833872
ACATGGG384800.024.7047353
ACGCAGA431700.024.4910457
CGCAGAG440700.023.830958
TACCTGG91500.023.383272
CATGGGG223500.022.0762754
GTACCTG102350.021.9622331
GCAGAGT502200.020.4634539
AGAGTAC454100.019.60941110-11
TATACCG8600.019.1242145
GAGTACT306500.018.5969112-13
ATGGGGG138450.017.852825
Produced by FastQC (version 0.11.5)