##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12128884 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08888913440016 33.0 31.0 34.0 30.0 34.0 2 32.3119768479936 34.0 31.0 34.0 30.0 34.0 3 32.33052587525777 34.0 31.0 34.0 30.0 34.0 4 35.97402044573928 37.0 35.0 37.0 35.0 37.0 5 35.88695546927483 37.0 35.0 37.0 35.0 37.0 6 35.90231030323977 37.0 35.0 37.0 35.0 37.0 7 35.824462168159904 37.0 35.0 37.0 35.0 37.0 8 35.81539266102306 37.0 35.0 37.0 35.0 37.0 9 37.46873265504065 39.0 37.0 39.0 34.0 39.0 10-11 37.429205193157095 39.0 37.0 39.0 34.0 39.0 12-13 37.34319179736569 39.0 37.0 39.0 34.0 39.0 14-15 38.57289677269566 40.0 38.0 41.0 34.0 41.0 16-17 38.50371856141093 40.0 38.0 41.0 34.0 41.0 18-19 38.48321782943921 40.0 38.0 41.0 34.0 41.0 20-21 38.422419531755764 40.0 38.0 41.0 34.0 41.0 22-23 38.31742891596622 40.0 38.0 41.0 34.0 41.0 24-25 38.21052402677773 40.0 38.0 41.0 33.0 41.0 26-27 38.03758169341879 40.0 37.0 41.0 33.0 41.0 28-29 38.077877321606834 40.0 37.5 41.0 33.0 41.0 30-31 38.011900023118365 40.0 37.0 41.0 33.0 41.0 32-33 37.84925435019413 40.0 37.0 41.0 33.0 41.0 34-35 37.74610743247277 40.0 37.0 41.0 33.0 41.0 36-37 37.69773047545017 40.0 37.0 41.0 33.0 41.0 38-39 37.607817875082326 40.0 37.0 41.0 32.0 41.0 40-41 37.45338404588584 40.0 37.0 41.0 31.5 41.0 42-43 37.30976436084309 40.0 36.0 41.0 31.0 41.0 44-45 37.135184778747984 39.5 36.0 41.0 31.0 41.0 46-47 36.89662165125827 39.0 36.0 41.0 30.0 41.0 48-49 36.693359628140556 39.0 35.5 41.0 30.0 41.0 50-51 36.50213222420133 39.0 35.0 40.5 29.5 41.0 52-53 36.30673357911577 39.0 35.0 40.0 29.0 41.0 54-55 36.11162651073256 39.0 35.0 40.0 28.0 41.0 56-57 35.91319646556106 38.0 35.0 40.0 28.0 41.0 58-59 35.65291134782062 38.0 34.0 40.0 28.0 41.0 60-61 35.34187543553059 38.0 34.0 40.0 27.0 41.0 62-63 35.02012608909443 37.5 34.0 40.0 26.0 41.0 64-65 34.7055801259209 37.0 33.0 40.0 26.0 41.0 66-67 34.3620858687411 36.5 33.0 39.0 26.0 41.0 68-69 33.96390277951376 36.0 32.5 39.0 25.5 40.0 70-71 33.573291945079205 35.5 32.0 39.0 25.0 40.0 72-73 33.16037089644851 35.0 31.5 38.0 24.0 40.0 74-75 32.74840846857798 35.0 31.0 37.0 23.5 39.0 76-77 31.209073110106424 33.5 29.5 35.5 21.5 38.5 78-79 32.034698328387016 34.0 31.0 36.0 23.0 39.0 80-81 31.992324932780292 34.0 31.0 36.0 23.0 37.5 82-83 31.699096883109775 34.0 31.0 35.5 22.0 37.0 84-85 31.387931898763316 34.0 30.5 35.0 21.5 37.0 86-87 31.07972184415318 34.0 30.0 35.0 20.0 36.0 88-89 30.788688225561394 34.0 30.0 35.0 20.0 36.0 90-91 30.498889386690482 34.0 29.0 35.0 19.5 35.5 92-93 30.277206212871686 34.0 29.0 35.0 19.0 35.0 94-95 30.045379525436964 34.0 29.0 35.0 18.0 35.0 96-97 29.829300041124974 34.0 29.0 35.0 18.0 35.0 98-99 29.65583585431273 34.0 29.0 35.0 18.0 35.0 100 27.75847085354267 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3393627384692053 1101 2 0.28331245549034634 1101 3 0.3031370370063513 1101 4 0.19158492518416637 1101 5 0.21502889788841628 1101 6 0.18630428225844042 1101 7 0.22328367900749413 1101 8 0.21360529822484153 1101 9 0.2568189175788831 1101 10-11 0.2881704206983926 1101 12-13 0.27225059569276766 1101 14-15 0.3257732928997825 1101 16-17 0.34613775008107694 1101 18-19 0.36220542462894656 1101 20-21 0.36742973075013197 1101 22-23 0.35048335120497853 1101 24-25 0.32264994346665077 1101 26-27 0.29381469325112874 1101 28-29 0.4041581306873212 1101 30-31 0.4220997676211269 1101 32-33 0.48808422880564706 1101 34-35 0.4192805432606903 1101 36-37 0.4704818752471809 1101 38-39 0.39807484157449124 1101 40-41 0.4401894063777192 1101 42-43 0.4914698871158407 1101 44-45 0.5246159122713436 1101 46-47 0.530983663954494 1101 48-49 0.5561205853182969 1101 50-51 0.47092005967574835 1101 52-53 0.45737816542281706 1101 54-55 0.43231989880924715 1101 56-57 0.4360410480645456 1101 58-59 0.44638850026118604 1101 60-61 0.42889056246677626 1101 62-63 0.4109299975936409 1101 64-65 0.41759998411991717 1101 66-67 0.38458091075058576 1101 68-69 0.37381072776982904 1101 70-71 0.3751208877202714 1101 72-73 0.23573187409144936 1101 74-75 0.2958645940262272 1101 76-77 0.3463093087998068 1101 78-79 0.4214377880144653 1101 80-81 0.40928821517622893 1101 82-83 0.3821808202133177 1101 84-85 0.3926114776719878 1101 86-87 0.3543080494958559 1101 88-89 0.3363413408882856 1101 90-91 0.20600558331063468 1101 92-93 0.2261138533667406 1101 94-95 0.26594100679629307 1101 96-97 0.20990377929294013 1101 98-99 0.15352096405675653 1101 100 0.2592014459294596 1102 1 0.304623239155454 1102 2 0.2127777005756002 1102 3 0.3209192783179873 1102 4 0.1932673272403207 1102 5 0.18601677471398403 1102 6 0.18562319538476402 1102 7 0.17661642017442603 1102 8 0.21412713054051125 1102 9 0.22010085659911738 1102 10-11 0.2436128119809382 1102 12-13 0.2903123490974835 1102 14-15 0.36021924004606376 1102 16-17 0.38781688567095074 1102 18-19 0.36040247850558416 1102 20-21 0.40014582611949123 1102 22-23 0.33712874763650547 1102 24-25 0.33640953154806397 1102 26-27 0.3102651045680034 1102 28-29 0.3729810853493376 1102 30-31 0.42641617359131345 1102 32-33 0.4950614007908314 1102 34-35 0.47530903601572305 1102 36-37 0.49016210538979266 1102 38-39 0.4748976587010887 1102 40-41 0.5371853462174272 1102 42-43 0.459285308848159 1102 44-45 0.5223149446523436 1102 46-47 0.5242646001122679 1102 48-49 0.5361933644793879 1102 50-51 0.4737368137824802 1102 52-53 0.5030619886783327 1102 54-55 0.4953703198682078 1102 56-57 0.4927850402021008 1102 58-59 0.5518883412358448 1102 60-61 0.5081235159540114 1102 62-63 0.5423649505020833 1102 64-65 0.44534804214840307 1102 66-67 0.5402879144554475 1102 68-69 0.5519342575018342 1102 70-71 0.5472154948990422 1102 72-73 0.5601895977082876 1102 74-75 0.5517408809136555 1102 76-77 0.5465393081407832 1102 78-79 0.597955333809459 1102 80-81 0.5949896028513386 1102 82-83 0.5079528667117579 1102 84-85 0.4899488205449316 1102 86-87 0.45920964003571285 1102 88-89 0.46058195911000865 1102 90-91 0.4255757564619991 1102 92-93 0.38841728516206686 1102 94-95 0.4069230097131893 1102 96-97 0.427868226386213 1102 98-99 0.42464119007330225 1102 100 0.508873452599925 1103 1 0.29684717403002026 1103 2 0.23851291753972959 1103 3 0.3082696355528256 1103 4 0.14192690401257124 1103 5 0.17593889735049117 1103 6 0.20794076377504922 1103 7 0.22147375910171263 1103 8 0.19862572522415434 1103 9 0.25126566157464936 1103 10-11 0.2588590214984805 1103 12-13 0.2831405503654949 1103 14-15 0.35588458677960944 1103 16-17 0.3551616990744506 1103 18-19 0.37682504077689316 1103 20-21 0.3689809369764774 1103 22-23 0.3655240778002806 1103 24-25 0.3495208329400228 1103 26-27 0.34906608831750674 1103 28-29 0.37494403931366094 1103 30-31 0.4790978056099675 1103 32-33 0.4658233577632913 1103 34-35 0.49253660411984157 1103 36-37 0.48259645896390424 1103 38-39 0.46517763340595053 1103 40-41 0.5006622991975505 1103 42-43 0.5187936529315422 1103 44-45 0.46711437635351416 1103 46-47 0.57228091289209 1103 48-49 0.5291526145134782 1103 50-51 0.5566646188460993 1103 52-53 0.5287251582797339 1103 54-55 0.5783301646858163 1103 56-57 0.5981147494707884 1103 58-59 0.6846580517165464 1103 60-61 0.6274249146491968 1103 62-63 0.6285520570541863 1103 64-65 0.6307053976286241 1103 66-67 0.5752640473637527 1103 68-69 0.5998815810040341 1103 70-71 0.6434224032535738 1103 72-73 0.5771659258851471 1103 74-75 0.5801869640677566 1103 76-77 0.6769711265904057 1103 78-79 0.6748558775874898 1103 80-81 0.6024577833355913 1103 82-83 0.6422072634806248 1103 84-85 0.558686577683595 1103 86-87 0.6204859781443375 1103 88-89 0.6318045085878623 1103 90-91 0.638802142854697 1103 92-93 0.5766565632070915 1103 94-95 0.6295006556161127 1103 96-97 0.6627883037529649 1103 98-99 0.6357090453344121 1103 100 0.7418282193129215 1104 1 0.2659680422707993 1104 2 0.2580860962382445 1104 3 0.3110107144611547 1104 4 0.10929418340455044 1104 5 0.18325916330935854 1104 6 0.17278159669810123 1104 7 0.19041005926795407 1104 8 0.20004531706310757 1104 9 0.23656209621223212 1104 10-11 0.26930387915840726 1104 12-13 0.3073147692910325 1104 14-15 0.4175484527133406 1104 16-17 0.35937528762486437 1104 18-19 0.3770828201008385 1104 20-21 0.2550326370597915 1104 22-23 0.3663249031914191 1104 24-25 0.34596147774605157 1104 26-27 0.41391988508510735 1104 28-29 0.49316168248445535 1104 30-31 0.3771621578178497 1104 32-33 0.4056965946465283 1104 34-35 0.4665628182115782 1104 36-37 0.36382588013220385 1104 38-39 0.4437547446590244 1104 40-41 0.5227141604529848 1104 42-43 0.5746733611128079 1104 44-45 0.5198333025719464 1104 46-47 0.5465357969950944 1104 48-49 0.5532079096618929 1104 50-51 0.5384170469391449 1104 52-53 0.5326538135074728 1104 54-55 0.5521258947322352 1104 56-57 0.591096206539099 1104 58-59 0.5956918770656472 1104 60-61 0.5901426835026058 1104 62-63 0.5651780543208176 1104 64-65 0.6525193039571136 1104 66-67 0.6289469490134962 1104 68-69 0.699089757968764 1104 70-71 0.6579501994542483 1104 72-73 0.7350357876478384 1104 74-75 0.7613336517207046 1104 76-77 0.6908994438765994 1104 78-79 0.7501326901409229 1104 80-81 0.8337921698258803 1104 82-83 0.657807509827574 1104 84-85 0.7657813383011742 1104 86-87 0.798277607004497 1104 88-89 0.7979302766419671 1104 90-91 0.6995045594419089 1104 92-93 0.7366759422897964 1104 94-95 0.808227241014059 1104 96-97 0.7540997249113133 1104 98-99 0.6690826547445177 1104 100 0.7920337646285098 1105 1 0.21974685394118154 1105 2 0.13806074762036502 1105 3 0.19491656255856782 1105 4 0.10857451443314403 1105 5 0.07351834961926329 1105 6 0.12556131022416395 1105 7 0.14758205448138995 1105 8 0.13024865744354486 1105 9 0.12372007726154521 1105 10-11 0.1845337748781617 1105 12-13 0.22630080135617447 1105 14-15 0.27086497409037236 1105 16-17 0.31033588199736073 1105 18-19 0.276432074386193 1105 20-21 0.1989978729576123 1105 22-23 0.32928001639756843 1105 24-25 0.3389536226685621 1105 26-27 0.36661533697044035 1105 28-29 0.4030162508480686 1105 30-31 0.4314921128445448 1105 32-33 0.4280727129407893 1105 34-35 0.44055348767150804 1105 36-37 0.5063275153085982 1105 38-39 0.4512810395649396 1105 40-41 0.5120677257746067 1105 42-43 0.4607463488342134 1105 44-45 0.4645249953951378 1105 46-47 0.5212300566975117 1105 48-49 0.5759979939681088 1105 50-51 0.6482160060036364 1105 52-53 0.5960253640425535 1105 54-55 0.5304886337038042 1105 56-57 0.5208493337585338 1105 58-59 0.6394362060466179 1105 60-61 0.6486695601598029 1105 62-63 0.597365992329081 1105 64-65 0.6258304662634586 1105 66-67 0.6144066061524569 1105 68-69 0.6814643439998349 1105 70-71 0.6512793442670315 1105 72-73 0.6446150748705506 1105 74-75 0.665940154467414 1105 76-77 0.606083410788866 1105 78-79 0.7291473964838247 1105 80-81 0.7333338787954808 1105 82-83 0.7580739179137446 1105 84-85 0.7366101951436832 1105 86-87 0.7775187632251956 1105 88-89 0.7702973882810369 1105 90-91 0.7590434392319665 1105 92-93 0.8172122710459888 1105 94-95 0.7854658253848896 1105 96-97 0.887023953772907 1105 98-99 0.8872178781849378 1105 100 0.9630354573056117 1106 1 0.22390058533082424 1106 2 0.18738853544983414 1106 3 0.23266791731030878 1106 4 0.17119714398271668 1106 5 0.17010870574303283 1106 6 0.15178877987951012 1106 7 0.1772224591303484 1106 8 0.15351592029409744 1106 9 0.1444193604631252 1106 10-11 0.2132963766331244 1106 12-13 0.24078904908835597 1106 14-15 0.29824863815208147 1106 16-17 0.38967392897426834 1106 18-19 0.31947936913108776 1106 20-21 0.3316446554912815 1106 22-23 0.3541702194583607 1106 24-25 0.3560538578995889 1106 26-27 0.3435335132564461 1106 28-29 0.38311429144171427 1106 30-31 0.3917191675530418 1106 32-33 0.4864678443775503 1106 34-35 0.5100942715277554 1106 36-37 0.5158430061469446 1106 38-39 0.4494552213448699 1106 40-41 0.4677157777310015 1106 42-43 0.47860149640496275 1106 44-45 0.49781961327298774 1106 46-47 0.5477866004944403 1106 48-49 0.5441687475489658 1106 50-51 0.6101243271572372 1106 52-53 0.5816893059069557 1106 54-55 0.5210677494760034 1106 56-57 0.5872505969692057 1106 58-59 0.6795510313388959 1106 60-61 0.663289221225611 1106 62-63 0.6497247402029345 1106 64-65 0.6690862784548273 1106 66-67 0.6530487258263875 1106 68-69 0.5912347894303025 1106 70-71 0.6178142418817529 1106 72-73 0.6500456180940546 1106 74-75 0.644602345330128 1106 76-77 0.6269070594742878 1106 78-79 0.7597336850410201 1106 80-81 0.7048400323462936 1106 82-83 0.7250384249191093 1106 84-85 0.6773514548411299 1106 86-87 0.6527944897422486 1106 88-89 0.7011483515054913 1106 90-91 0.7118166365339924 1106 92-93 0.7235099629662862 1106 94-95 0.8283791589276035 1106 96-97 0.7650482173477968 1106 98-99 0.7881442120461841 1106 100 0.8960591421875428 1107 1 0.24498343763472263 1107 2 0.20077246664187953 1107 3 0.26184078324541105 1107 4 0.16027521159195857 1107 5 0.14257705969215806 1107 6 0.15213384362277083 1107 7 0.18337152511286803 1107 8 0.18393570307004126 1107 9 0.18765520032015814 1107 10-11 0.1911647137185426 1107 12-13 0.21183658562792118 1107 14-15 0.2693649559446172 1107 16-17 0.2690356497610793 1107 18-19 0.21233277643489146 1107 20-21 0.29393327416669734 1107 22-23 0.3443947229550375 1107 24-25 0.31349428958326797 1107 26-27 0.35633594250791134 1107 28-29 0.38987665200147603 1107 30-31 0.3002186074855686 1107 32-33 0.3206582102032485 1107 34-35 0.35732050549687955 1107 36-37 0.4616817783614451 1107 38-39 0.4741675541673871 1107 40-41 0.4385334998912427 1107 42-43 0.40774469878778774 1107 44-45 0.41620362303837766 1107 46-47 0.4228088671683494 1107 48-49 0.4536230480205603 1107 50-51 0.4461992536911481 1107 52-53 0.46111340379616905 1107 54-55 0.4787660707267847 1107 56-57 0.5190718326255492 1107 58-59 0.5735449266119375 1107 60-61 0.5244067252222919 1107 62-63 0.5628913104693964 1107 64-65 0.7403975341972355 1107 66-67 0.7277874102922013 1107 68-69 0.6473476947737495 1107 70-71 0.7154556473004732 1107 72-73 0.7121324806804168 1107 74-75 0.7108376274008563 1107 76-77 0.6334199876631459 1107 78-79 0.7039907841433077 1107 80-81 0.7366869099511426 1107 82-83 0.7714080995257397 1107 84-85 0.6372641505542589 1107 86-87 0.5722143412909944 1107 88-89 0.5870372873130876 1107 90-91 0.457561965601716 1107 92-93 0.5450625064870493 1107 94-95 0.6987957092127743 1107 96-97 0.7152245596045255 1107 98-99 0.8210125854612187 1107 100 0.9759297648281269 1108 1 0.21500726432356032 1108 2 0.1444178626924284 1108 3 0.20782221563045056 1108 4 0.1519705540479137 1108 5 0.12120295794280622 1108 6 0.1326979681018301 1108 7 0.15144204528478866 1108 8 0.13324452401509745 1108 9 0.12170026983040572 1108 10-11 0.15704048967216977 1108 12-13 0.1835166165531419 1108 14-15 0.2147581485264709 1108 16-17 0.26323846176115495 1108 18-19 0.27902500444108114 1108 20-21 0.274655535705854 1108 22-23 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-0.3215660714763757 2316 64-65 -0.3605431609718508 2316 66-67 -0.09924253501053215 2316 68-69 -0.09827774770131015 2316 70-71 -0.35671622043563644 2316 72-73 -0.4392613073844487 2316 74-75 -0.430832369660088 2316 76-77 -0.39814426761703103 2316 78-79 -0.2929064297091024 2316 80-81 -0.2312413278809764 2316 82-83 -0.13276780728423532 2316 84-85 -0.18190759606975604 2316 86-87 -0.24728806870525588 2316 88-89 -0.17499665483993 2316 90-91 -0.1418827284687758 2316 92-93 -0.03929894954328361 2316 94-95 0.07669601060859321 2316 96-97 -0.020004215577621665 2316 98-99 -0.0700489687718644 2316 100 -0.2596851614399718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 9.0 10 25.0 11 59.0 12 117.0 13 377.0 14 1019.0 15 2828.0 16 6239.0 17 12644.0 18 22479.0 19 34806.0 20 50183.0 21 69918.0 22 90614.0 23 105987.0 24 117575.0 25 133603.0 26 158540.0 27 191606.0 28 231961.0 29 280534.0 30 338476.0 31 408447.0 32 496177.0 33 619483.0 34 804906.0 35 1083163.0 36 1581958.0 37 2440236.0 38 2457301.0 39 387568.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34720350593216 20.506202265215272 13.663666783416739 24.48292744543583 2 14.699072842284021 21.68464658469834 39.87498414944019 23.74129642357745 3 16.96592997286455 27.161156468777087 30.88887558719819 24.984037971160173 4 11.729488316534347 17.76345356967095 38.86490237663111 31.642155737163595 5 12.271459657051796 38.68924416706407 35.95961384852736 13.079682327356771 6 30.36956927886319 38.77648629025968 17.934124187823024 12.919820243054106 7 26.363670392098733 33.02007835180879 23.266724292193743 17.34952696389874 8 25.20074378529264 36.78472234074208 20.61011302960754 17.40442084435774 9 26.156710265086307 15.160146691203144 20.510418109503927 38.17272493420662 10-11 24.113982786874704 26.76935899461154 29.51191140091702 19.604746817596737 12-13 24.750067689657186 24.417427852389388 29.355734624883873 21.476769833069557 14-15 22.05460947602434 25.911864603536483 26.638440107103012 25.395085813336166 16-17 21.765499594003312 28.836153406354885 27.32292375164933 22.07542324799247 18-19 21.647339934808635 28.111981993862766 29.05218166071229 21.18849641061631 20-21 22.35878861284698 27.315402140867878 29.022032210515857 21.303777035769283 22-23 22.127666486050984 27.20723522460929 28.765952415737505 21.899145873602222 24-25 21.945996845216754 27.36243911641005 28.90604774520063 21.785516293172563 26-27 21.94071474032631 27.54310553257388 28.828477088110414 21.68770263898939 28-29 21.877043305871798 27.42999142741656 28.998697931964728 21.694267334746915 30-31 22.167283653724265 27.39950209826718 28.8987066501818 21.534507597826757 32-33 22.006242699313006 27.48955719626411 28.812673054752757 21.69152704967013 34-35 22.1400583407395 27.5763016779703 28.850504885103174 21.433135096187026 36-37 22.174665659140313 27.497426561676154 28.746379975125148 21.581527804058386 38-39 22.055868035062108 27.65662191594792 28.739820689040503 21.54768935994947 40-41 22.122348811971264 27.43409968237771 28.859747356624315 21.583804149026708 42-43 21.964750802425197 27.46800142067034 28.733906919707596 21.833340857196866 44-45 21.996126731472895 27.649943627518482 28.615230645654137 21.738698995354486 46-47 22.01842279402413 27.58499113647357 28.429556433674318 21.96702963582798 48-49 21.96133854008628 27.712358507368172 28.450400071241045 21.8759028813045 50-51 22.10946201884662 27.80610628404432 28.21617895108453 21.868252746024535 52-53 22.075146156737013 27.84504129062834 28.14378181886541 21.936030733769236 54-55 21.9942282758418 27.808834506736407 28.258172833716372 21.938764383705422 56-57 22.084280581064906 27.86422937795001 28.05137032354792 22.000119717437165 58-59 22.028426169430084 27.778062232406526 28.24268382694869 21.95082777121471 60-61 22.18400568768015 27.895160504016854 28.158654778717047 21.762179029585955 62-63 22.00612441504423 27.876152605395525 28.264486300587627 21.853236678972614 64-65 21.933020154044105 27.7181315489059 28.243752818537338 22.10509547851266 66-67 21.91568887114983 27.815382575547222 28.082577489060444 22.18635106424251 68-69 21.853669489443355 27.86888145455217 28.180746488013902 22.096702567990572 70-71 21.91829400985527 27.64307024368231 28.156174815128622 22.282460931333794 72-73 21.86359919188508 27.877858122763744 27.93327956234749 22.325263123003687 74-75 21.818192986433278 27.91241960643809 27.92740904889885 22.341978358229785 76-77 21.743128014095355 27.806209579054286 28.046749749794607 22.40391265705575 78-79 21.768166537900534 27.734048596916374 28.00815143227245 22.489633432910644 80-81 21.740296961781507 28.035918061766996 27.74733347845015 22.476451498001346 82-83 21.833837625569014 27.929527231904416 27.83684894825115 22.39978619427542 84-85 21.738551944886257 27.90877393958625 27.8165400267969 22.53613408873059 86-87 21.631150930210964 27.88801727568811 27.93751763746751 22.54331415663341 88-89 21.60475684847573 27.805688076904932 27.9490329652423 22.640522109377038 90-91 21.736578964773383 27.84035496612981 27.864769443382905 22.5582966257139 92-93 21.676206327186712 27.77153392792635 27.82109755031415 22.731162194572786 94-95 21.53372439338816 27.70770460339599 27.84238443259864 22.916186570617214 96-97 21.515574319097723 27.676784553273283 27.88716106624743 22.920480061381568 98-99 21.417355034042444 27.60727866578382 27.81303949907891 23.162326801094824 100 20.55827962997521 28.131172615150753 28.020251599638385 23.29029615523566 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2593.0 1 1943.0 2 1959.0 3 2725.0 4 2334.5 5 2246.0 6 3550.0 7 6821.0 8 10720.5 9 12600.0 10 11186.0 11 9254.5 12 9455.5 13 10168.5 14 11020.0 15 11863.0 16 12544.0 17 13455.0 18 14727.0 19 16312.5 20 18539.0 21 21729.5 22 26454.0 23 33631.0 24 42762.5 25 54019.0 26 69198.5 27 86991.5 28 106055.0 29 128340.5 30 153439.0 31 177480.0 32 204716.5 33 235705.0 34 266800.5 35 296746.0 36 333503.5 37 367038.5 38 388990.0 39 407906.5 40 414338.5 41 417832.0 42 426675.0 43 437159.0 44 446663.5 45 457115.5 46 463947.5 47 465357.0 48 469467.0 49 467245.5 50 457062.0 51 439051.0 52 416337.5 53 395376.0 54 371237.5 55 339727.5 56 302195.5 57 263978.0 58 227453.5 59 192408.5 60 158949.5 61 128854.5 62 102857.0 63 78456.5 64 56968.5 65 41116.5 66 29484.0 67 21349.0 68 15486.0 69 11059.0 70 8016.0 71 5732.5 72 4102.0 73 3006.5 74 2173.5 75 1437.5 76 950.5 77 643.0 78 424.0 79 260.5 80 184.0 81 135.0 82 97.0 83 74.5 84 50.0 85 31.0 86 21.5 87 17.5 88 14.5 89 12.5 90 9.0 91 4.5 92 5.0 93 6.0 94 4.5 95 5.5 96 6.5 97 7.5 98 6.0 99 5.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033968500317094305 2 0.06481222839628113 3 8.244781630362694E-4 4 6.348481855379274E-4 5 0.0014181024404223833 6 5.111764610824871E-4 7 0.0 8 9.481498874917099E-4 9 3.297912652145078E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.461527375478238E-4 18-19 0.0010759440027623317 20-21 0.003227832008286995 22-23 0.0 24-25 0.0 26-27 1.4428367853134714E-4 28-29 8.368453354818135E-4 30-31 0.0015294069924322799 32-33 0.002609473386009793 34-35 0.003965739964204456 36-37 0.0013933680955312954 38-39 0.0014634487393893783 40-41 0.00529314980669285 42-43 0.006649416384887513 44-45 0.0051612333006070465 46-47 0.0066823955114089645 48-49 0.008607552022098654 50-51 0.008520981814979844 52-53 0.0038420682397490155 54-55 0.0022467029942738344 56-57 0.0026795540298678758 58-59 0.0033968500317094305 60-61 0.00755634236422741 62-63 0.01389245704716114 64-65 0.003912148883607099 66-67 0.010062755979857669 68-69 0.012552680032227202 70-71 0.005396209577072384 72-73 0.007469772157108601 74-75 0.0028650616165510363 76-77 0.0028733063981813993 78-79 0.00786552167536601 80-81 0.005969221900382591 82-83 0.016382381099530677 84-85 0.01737999967680456 86-87 0.022343358218282903 88-89 0.020908766214599795 90-91 0.023761460658705286 92-93 0.033506792545793994 94-95 0.037711631177278965 96-97 0.02282155555284394 98-99 0.018002480689896943 100 0.018443576507121348 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2128884E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.525248725324225 #Duplication Level Percentage of deduplicated Percentage of total 1 70.83562812233247 39.331658701070815 2 16.666296195570183 18.508004831779186 3 5.880271246386089 9.795105705838795 4 2.590071336222227 5.7525742064028815 5 1.2986348944345592 3.6053512758432036 6 0.7697646040905177 2.5644822661246036 7 0.48950311184815903 1.9025847426032465 8 0.3077329120139848 1.3669557184435859 9 0.22503227916185997 1.1245475944519985 >10 0.864917873946553 8.063063444762616 >50 0.04003512114762339 1.5331102945990678 >100 0.02795819351907896 3.0602861798472905 >500 0.002730025264279542 0.9911690949652673 >1k 0.001329145131958935 1.2129080967664332 >5k 2.373473270929917E-5 0.07175839922579877 >10k+ 7.12041981278975E-5 1.1164394472752144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34377 0.2834308581069784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29221 0.24092076402082832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21117 0.174105053688369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.244781630362694E-6 0.0 5 8.244781630362694E-6 8.244781630362694E-6 0.0 8.244781630362694E-6 0.0 6 3.297912652145078E-5 8.244781630362694E-6 0.0 8.244781630362694E-6 0.0 7 3.297912652145078E-5 8.244781630362694E-6 0.0 8.244781630362694E-6 0.0 8 3.297912652145078E-5 8.244781630362694E-6 0.0 8.244781630362694E-6 8.244781630362694E-6 9 3.297912652145078E-5 8.244781630362694E-6 0.0 5.771347141253886E-5 3.297912652145078E-5 10-11 4.122390815181347E-5 8.244781630362694E-6 0.0 5.771347141253886E-5 3.297912652145078E-5 12-13 4.122390815181347E-5 2.0611954075906735E-5 0.0 6.595825304290155E-5 3.297912652145078E-5 14-15 5.359108059735751E-5 4.122390815181347E-5 0.0 7.00806438580829E-5 6.595825304290155E-5 16-17 6.595825304290155E-5 4.946868978217617E-5 0.0 7.420303467326425E-5 8.244781630362694E-5 18-19 6.595825304290155E-5 4.946868978217617E-5 0.0 7.83254254884456E-5 9.069259793398964E-5 20-21 6.595825304290155E-5 4.946868978217617E-5 0.0 8.244781630362694E-5 9.069259793398964E-5 22-23 6.595825304290155E-5 4.946868978217617E-5 0.0 1.319165060858031E-4 1.1130455200989637E-4 24-25 7.83254254884456E-5 5.359108059735752E-5 0.0 2.6795540298678755E-4 1.401612877161658E-4 26-27 8.244781630362694E-5 6.595825304290155E-5 0.0 4.781973345610363E-4 1.401612877161658E-4 28-29 8.244781630362694E-5 7.420303467326424E-5 0.0 0.0014057352679768395 1.401612877161658E-4 30-31 8.657020711880829E-5 8.244781630362694E-5 0.0 0.005012827231260519 1.401612877161658E-4 32-33 9.893737956435234E-5 8.244781630362694E-5 0.0 0.013191650608580312 1.484060693465285E-4 34-35 1.0305977037953367E-4 9.893737956435234E-5 0.0 0.026395668389606164 1.6489563260725388E-4 36-37 1.0718216119471502E-4 1.1954933364025907E-4 0.0 0.04897812527516959 1.6489563260725388E-4 38-39 1.0718216119471502E-4 1.5252846016170984E-4 0.0 0.08459145952752126 1.6489563260725388E-4 40-41 1.0718216119471502E-4 1.6489563260725388E-4 0.0 0.12417465613489254 1.6489563260725388E-4 42-43 1.0718216119471502E-4 1.8138519586797928E-4 0.0 0.16225730248553782 1.6489563260725388E-4 44-45 1.1954933364025907E-4 1.8138519586797928E-4 0.0 0.21054286610375694 1.6901802342243522E-4 46-47 1.319165060858031E-4 1.9787475912870468E-4 0.0 0.27154188299599535 1.8138519586797928E-4 48-49 1.319165060858031E-4 1.9787475912870468E-4 0.0 0.33806902597139193 1.8962997749834198E-4 50-51 1.319165060858031E-4 2.102419315742487E-4 0.0 0.41004596960445827 1.8962997749834198E-4 52-53 1.401612877161658E-4 2.3085388565015545E-4 0.0 0.4801060015084653 2.184867132046114E-4 54-55 1.401612877161658E-4 2.473434489108808E-4 0.0 0.5615232201082969 2.514658397260622E-4 56-57 1.401612877161658E-4 2.514658397260622E-4 0.0 0.6587085835761972 2.5558823054124355E-4 58-59 1.401612877161658E-4 2.5558823054124355E-4 0.0 0.7696998338841397 2.5558823054124355E-4 60-61 1.401612877161658E-4 2.597106213564249E-4 0.0 0.8906095564934087 2.5558823054124355E-4 62-63 1.484060693465285E-4 2.638330121716062E-4 0.0 1.0151263710659613 2.7207779380196894E-4 64-65 1.566508509768912E-4 2.638330121716062E-4 0.0 1.1434893762690779 2.7207779380196894E-4 66-67 1.566508509768912E-4 2.803225754323316E-4 0.0 1.295906531878778 2.84444966247513E-4 68-69 1.8138519586797928E-4 2.803225754323316E-4 0.0 1.469690039083563 2.96812138693057E-4 70-71 1.8138519586797928E-4 2.803225754323316E-4 0.0 1.663982440593875 2.96812138693057E-4 72-73 1.8138519586797928E-4 2.803225754323316E-4 0.0 1.8640997803260384 3.215464835841451E-4 74-75 1.8138519586797928E-4 2.8444496624751295E-4 0.0 2.0638090033674987 3.215464835841451E-4 76-77 1.8138519586797928E-4 3.050569203234197E-4 0.0 2.2823122061353707 3.215464835841451E-4 78-79 1.8962997749834198E-4 3.050569203234197E-4 0.0 2.5320961104088386 3.215464835841451E-4 80-81 1.8962997749834198E-4 3.050569203234197E-4 0.0 2.812769089060461 3.380360468448705E-4 82-83 1.9375236831352334E-4 3.050569203234197E-4 0.0 3.1051331680639374 3.586480009207772E-4 84-85 1.9787475912870468E-4 3.050569203234197E-4 0.0 3.3988700032088692 3.6277039173595856E-4 86-87 1.9787475912870468E-4 3.050569203234197E-4 0.0 3.7001673031088433 3.6277039173595856E-4 88 1.9787475912870468E-4 3.050569203234197E-4 0.0 3.9448147084265957 3.7925995499668396E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16715 0.0 48.221516 1 GTATCAA 29115 0.0 37.43976 1 TCAACGC 35770 0.0 29.689543 4 ATCAACG 36095 0.0 29.33145 3 CAACGCA 36270 0.0 29.24139 5 AACGCAG 38065 0.0 27.99781 6 TATCAAC 38655 0.0 27.72385 2 GTACATG 37465 0.0 26.99921 1 TACATGG 38935 0.0 25.483387 2 ACATGGG 38480 0.0 24.704735 3 ACGCAGA 43170 0.0 24.491045 7 CGCAGAG 44070 0.0 23.83095 8 TACCTGG 9150 0.0 23.38327 2 CATGGGG 22350 0.0 22.076275 4 GTACCTG 10235 0.0 21.962233 1 GCAGAGT 50220 0.0 20.463453 9 AGAGTAC 45410 0.0 19.609411 10-11 TATACCG 860 0.0 19.124214 5 GAGTACT 30650 0.0 18.59691 12-13 ATGGGGG 13845 0.0 17.85282 5 >>END_MODULE