FastQCFastQC Report
Mon 6 Jun 2016
ERR1211511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791423
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126760.45410530757968254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107610.38550230473847924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69510.24901277950350054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65100.23321438563771954No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35370.1267095671275905No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.12405858947210796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG54300.065.03232
GTACCTG60200.060.1487661
TATAACG4750.059.394562
ACCTGGG59300.058.6462553
TAACGCA5350.050.9464724
GTACCCG5050.043.8231281
CCTGGGG40050.039.7774624
ATAACGC7400.039.375243
TACCCGG5100.038.7229232
TATCACG3050.038.541552
GTATAAC11400.033.869271
GGTATCA101000.029.2309551
GTATCAA147900.028.5257241
CCTGGGA45200.028.487354
TCACGCA4500.027.1519324
CTGGGGG29900.025.9302045
CATGGGG65150.025.5345824
GTACACG4800.025.5052151
ATCACGC4800.025.4563053
TGGGGGG57750.024.8103076
Produced by FastQC (version 0.11.5)