##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791423 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02088576328274 31.0 31.0 34.0 28.0 34.0 2 31.030171708121628 31.0 31.0 34.0 27.0 34.0 3 31.12359001125949 31.0 31.0 34.0 27.0 34.0 4 34.65743959263788 37.0 35.0 37.0 31.0 37.0 5 34.767993958636865 37.0 35.0 37.0 32.0 37.0 6 34.88035098944159 37.0 35.0 37.0 32.0 37.0 7 34.82808983088554 37.0 35.0 37.0 32.0 37.0 8 34.793482750554105 37.0 35.0 37.0 31.0 37.0 9 36.30558965803463 39.0 37.0 39.0 31.0 39.0 10-11 36.26711949425078 39.0 37.0 39.0 31.0 39.0 12-13 36.205503787853004 39.0 37.0 39.0 31.0 39.0 14-15 37.32133324114618 40.0 37.0 41.0 31.0 41.0 16-17 37.29197258889104 40.0 37.0 41.0 31.0 41.0 18-19 37.32951294017424 40.0 37.0 41.0 31.0 41.0 20-21 37.28393493211169 40.0 37.0 41.0 31.0 41.0 22-23 37.117104072009155 40.0 36.5 41.0 30.0 41.0 24-25 37.0250546047661 40.0 36.0 41.0 30.0 41.0 26-27 36.865022069388985 40.0 36.0 41.0 30.0 41.0 28-29 36.68376738315905 39.5 36.0 41.0 29.5 41.0 30-31 36.469756643833634 39.0 35.5 41.0 29.0 41.0 32-33 36.235537752608614 39.0 35.0 41.0 28.5 41.0 34-35 36.03346321929711 39.0 35.0 40.0 27.0 41.0 36-37 35.84163829702628 39.0 35.0 40.0 27.0 41.0 38-39 35.69806027248468 38.5 35.0 40.0 26.0 41.0 40-41 35.66051615967913 38.5 35.0 40.0 26.0 41.0 42-43 35.5953676673152 38.5 35.0 40.0 26.0 41.0 44-45 35.39898772059985 38.0 34.0 40.0 25.0 41.0 46-47 35.30736438010291 38.0 34.0 40.0 24.0 41.0 48-49 35.24105715973538 39.0 34.0 40.0 24.0 41.0 50-51 34.287916055717815 38.0 33.0 39.5 23.0 40.5 52-53 34.51518329540166 38.0 33.5 39.5 23.0 40.5 54-55 35.101024101327525 39.0 34.0 40.0 24.0 41.0 56-57 35.072887018556486 39.0 34.0 40.0 23.0 41.0 58-59 34.92672715672258 38.0 34.0 40.0 23.0 41.0 60-61 34.73363030253745 38.0 34.0 40.0 23.0 41.0 62-63 34.46357520877345 38.0 33.0 40.0 22.0 41.0 64-65 34.20100017088059 37.0 33.0 40.0 22.0 41.0 66-67 33.904748402517285 37.0 33.0 40.0 22.0 41.0 68-69 33.62603929966902 36.0 32.0 39.0 22.0 41.0 70-71 33.27515804663069 36.0 32.0 39.0 21.0 41.0 72-73 32.922357521593824 35.0 32.0 39.0 20.0 40.0 74-75 32.5573377807663 35.0 31.0 37.5 20.0 40.0 76-77 32.20648823198778 35.0 31.0 37.0 20.0 39.0 78-79 31.882229063814407 35.0 31.0 36.5 19.5 39.0 80-81 31.554938108627752 35.0 31.0 36.0 18.5 38.5 82-83 31.245433064068038 35.0 30.0 36.0 18.0 37.0 84-85 30.962393732515636 34.0 30.0 35.0 18.0 37.0 86-87 30.660277034329802 34.0 30.0 35.0 18.0 36.5 88-89 30.43089116196291 34.0 29.0 35.0 17.0 36.0 90-91 30.189478986165838 34.0 29.0 35.0 16.0 36.0 92-93 30.01666318576583 34.0 29.0 35.0 15.0 36.0 94-95 29.85247452643329 34.0 29.0 35.0 15.0 35.0 96-97 29.742160718744525 34.0 29.0 35.0 13.5 35.0 98-99 29.555558580695223 34.0 29.0 35.0 11.5 35.0 100 28.185969306694112 32.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5111110937528132 1101 2 0.4327936412868958 1101 3 0.4139534520925636 1101 4 0.47000460191451054 1101 5 0.4081066951046921 1101 6 0.3793664090150273 1101 7 0.45211894262953223 1101 8 0.4286962424160947 1101 9 0.3346184975393598 1101 10-11 0.43476526933793735 1101 12-13 0.4799264584511249 1101 14-15 0.5761898899782452 1101 16-17 0.5322876058804695 1101 18-19 0.5923226620556576 1101 20-21 0.49978062764024145 1101 22-23 0.5531391256301248 1101 24-25 0.5485679470066103 1101 26-27 0.4468003522755879 1101 28-29 0.5034965857242426 1101 30-31 0.5708101847430171 1101 32-33 0.5123758681595021 1101 34-35 0.5312186608105236 1101 36-37 0.5315688688523963 1101 38-39 0.6117432182216689 1101 40-41 0.46211479819331913 1101 42-43 0.3734488706102681 1101 44-45 0.4247622732411287 1101 46-47 0.43864719410079545 1101 48-49 0.4804972921227204 1101 50-51 0.38968937165335404 1101 52-53 0.5570662985859585 1101 54-55 0.6139950571244128 1101 56-57 0.5654842828286846 1101 58-59 0.5355451925970627 1101 60-61 0.38169998102177516 1101 62-63 0.37867130663436654 1101 64-65 0.46526037238663775 1101 66-67 0.5076004014919846 1101 68-69 0.41615275384477 1101 70-71 0.4254580716599108 1101 72-73 0.530289749900497 1101 74-75 0.3854918418800892 1101 76-77 0.37389753823565286 1101 78-79 0.30811455128899823 1101 80-81 0.36505117677868526 1101 82-83 0.3426748060683451 1101 84-85 0.1763629058193743 1101 86-87 0.03912518606448501 1101 88-89 0.14826928105837212 1101 90-91 0.08700173313706472 1101 92-93 0.09052932814797643 1101 94-95 0.09215675144073643 1101 96-97 0.02999108766491787 1101 98-99 -0.03850441168884089 1101 100 0.28004236450939857 1102 1 0.36341545071940473 1102 2 0.406999265456939 1102 3 0.3292438314223993 1102 4 0.2954164491161677 1102 5 0.25122452891032765 1102 6 0.32575879310525124 1102 7 0.21740345271810213 1102 8 0.36482463886643757 1102 9 0.4072826887347034 1102 10-11 0.38494508255237037 1102 12-13 0.3177898291464416 1102 14-15 0.3838013011096493 1102 16-17 0.38795898407779816 1102 18-19 0.41504698257768524 1102 20-21 0.36232990550249866 1102 22-23 0.3997994499792057 1102 24-25 0.4534005763625899 1102 26-27 0.4027184817760272 1102 28-29 0.4252443462936739 1102 30-31 0.385404942924751 1102 32-33 0.3178982273159576 1102 34-35 0.42400906611534595 1102 36-37 0.37579964786097975 1102 38-39 0.3331913490507077 1102 40-41 0.2229697996379656 1102 42-43 0.2343195565245182 1102 44-45 0.18687717198400833 1102 46-47 0.0664178130990507 1102 48-49 0.12388528787741393 1102 50-51 0.1321500493948662 1102 52-53 0.26101480577595026 1102 54-55 0.3201353003784888 1102 56-57 0.37481905130756843 1102 58-59 0.3507166507089181 1102 60-61 0.2481448986899224 1102 62-63 0.24360812773392837 1102 64-65 0.14770319481301897 1102 66-67 0.11722865414037642 1102 68-69 -0.010700859761961112 1102 70-71 0.09578737699154516 1102 72-73 0.06584353547268762 1102 74-75 0.04371918069536207 1102 76-77 0.10007941662718167 1102 78-79 0.10575311567479417 1102 80-81 0.010724308011162975 1102 82-83 0.00808489139230062 1102 84-85 -0.15217168722335472 1102 86-87 -0.10770996651258002 1102 88-89 -0.08004492252759476 1102 90-91 -0.17168076478548855 1102 92-93 -0.12291469399110966 1102 94-95 -0.281532202647206 1102 96-97 -0.394156153581676 1102 98-99 -0.3464034266953071 1102 100 -0.1408142882810992 1103 1 0.4623109752756456 1103 2 0.5183384705968308 1103 3 0.4102421459062455 1103 4 0.4511558806696314 1103 5 0.42562910803631837 1103 6 0.4629136038657222 1103 7 0.5216720226467615 1103 8 0.5417155223070651 1103 9 0.5540544234724507 1103 10-11 0.5847219967085877 1103 12-13 0.5657276769781276 1103 14-15 0.6923960789426573 1103 16-17 0.62862969002461 1103 18-19 0.5248683379169776 1103 20-21 0.5875450693243636 1103 22-23 0.6200164072551075 1103 24-25 0.5808792146344857 1103 26-27 0.6260648910229349 1103 28-29 0.6414554429265635 1103 30-31 0.5892586452847652 1103 32-33 0.6675300919439309 1103 34-35 0.7388891457419291 1103 36-37 0.6158261929005633 1103 38-39 0.6183038246966888 1103 40-41 0.6054929073573092 1103 42-43 0.5446562725869839 1103 44-45 0.5735173846259016 1103 46-47 0.580032171738452 1103 48-49 0.6186417146343643 1103 50-51 0.5316228176378317 1103 52-53 0.5969349330231992 1103 54-55 0.6834141609896207 1103 56-57 0.672449174850577 1103 58-59 0.7536123937007133 1103 60-61 0.645877381902217 1103 62-63 0.6251348365468559 1103 64-65 0.6299158957953281 1103 66-67 0.6395978202551831 1103 68-69 0.47819188422057834 1103 70-71 0.5579826671242003 1103 72-73 0.569879313680822 1103 74-75 0.3867759004524629 1103 76-77 0.42453674346020875 1103 78-79 0.4648446904084942 1103 80-81 0.5010806662083525 1103 82-83 0.5515991632440951 1103 84-85 0.5892381585673299 1103 86-87 0.37748590501275814 1103 88-89 0.40596240295620944 1103 90-91 0.3332833112049123 1103 92-93 0.08946135476353234 1103 94-95 0.15969407408354996 1103 96-97 0.17101955531796165 1103 98-99 0.2152585948382999 1103 100 0.1840129284478209 1104 1 0.5207281439554308 1104 2 0.616561951979012 1104 3 0.5015394502308723 1104 4 0.48451667839773904 1104 5 0.4117148112992908 1104 6 0.42632419206525896 1104 7 0.45783239473302473 1104 8 0.45870744929455043 1104 9 0.5535436870327146 1104 10-11 0.5542906523489748 1104 12-13 0.46442874969348935 1104 14-15 0.6332622963863841 1104 16-17 0.5773949635767366 1104 18-19 0.5067495933831268 1104 20-21 0.5597418016241846 1104 22-23 0.5386232216610836 1104 24-25 0.6876351602819923 1104 26-27 0.7447157722754412 1104 28-29 0.7808061344046067 1104 30-31 0.7476247192157572 1104 32-33 0.7013718683274774 1104 34-35 0.6824595754527749 1104 36-37 0.8197530702157607 1104 38-39 0.7644664634397174 1104 40-41 0.736002793273137 1104 42-43 0.545877871842066 1104 44-45 0.6892645076179846 1104 46-47 0.7555173080417674 1104 48-49 0.6843231594528731 1104 50-51 0.752989075602791 1104 52-53 0.8309940995182075 1104 54-55 0.7608841204008456 1104 56-57 0.6900675243023642 1104 58-59 0.7145242249736512 1104 60-61 0.8413821203545098 1104 62-63 0.7706801067897473 1104 64-65 0.6624073863353104 1104 66-67 0.6178609723516502 1104 68-69 0.5144825046513901 1104 70-71 0.5513068986558025 1104 72-73 0.6985199991988722 1104 74-75 0.6790620812751129 1104 76-77 0.6613846997546773 1104 78-79 0.48215601761411975 1104 80-81 0.5129989226301248 1104 82-83 0.48586411220421866 1104 84-85 0.48309641569822404 1104 86-87 0.4808943637368124 1104 88-89 0.6248474737501901 1104 90-91 0.4757790417015322 1104 92-93 0.41167911800807033 1104 94-95 0.5226075067895941 1104 96-97 0.40144114925463015 1104 98-99 0.3398457319621677 1104 100 0.44575828370089354 1105 1 0.38258478456197054 1105 2 0.4323688369836347 1105 3 0.35079832316412407 1105 4 0.3695820650125583 1105 5 0.2628262909204011 1105 6 0.33378964214256257 1105 7 0.3797741695897301 1105 8 0.41193614547802326 1105 9 0.5180772715154589 1105 10-11 0.4300520755964996 1105 12-13 0.4520167490386129 1105 14-15 0.49576516570091655 1105 16-17 0.5541626934088271 1105 18-19 0.5732154713258595 1105 20-21 0.5497688238158034 1105 22-23 0.6399122401192159 1105 24-25 0.5477716890190223 1105 26-27 0.5622160803048004 1105 28-29 0.5652169164447258 1105 30-31 0.5923504971375664 1105 32-33 0.6547923444311508 1105 34-35 0.5961983809065003 1105 36-37 0.7437072683605024 1105 38-39 0.7275670784857269 1105 40-41 0.709860067660621 1105 42-43 0.7037455534021646 1105 44-45 0.6914069994657623 1105 46-47 0.5964439869283353 1105 48-49 0.7134827794206018 1105 50-51 0.617470506074234 1105 52-53 0.7465363535504395 1105 54-55 0.7661630262130643 1105 56-57 0.778784725505453 1105 58-59 0.7605924412389555 1105 60-61 0.7207348283122954 1105 62-63 0.8110900769826301 1105 64-65 0.7023368518326478 1105 66-67 0.6135537303705405 1105 68-69 0.6605412690570347 1105 70-71 0.6711693487803672 1105 72-73 0.7038039685073301 1105 74-75 0.6475990979643953 1105 76-77 0.7173874956485236 1105 78-79 0.6092951337977262 1105 80-81 0.5734286910933264 1105 82-83 0.6074979420977513 1105 84-85 0.5867665432645737 1105 86-87 0.4861860337791448 1105 88-89 0.4803452069917036 1105 90-91 0.4066252911698669 1105 92-93 0.6465684848347415 1105 94-95 0.5501654796019935 1105 96-97 0.5579228042077418 1105 98-99 0.6410050827206817 1105 100 0.694044501201688 1106 1 0.4519831575345812 1106 2 0.3983335978945597 1106 3 0.412823317382923 1106 4 0.34780232324599325 1106 5 0.3695776967075659 1106 6 0.3329734726591411 1106 7 0.3647715494265853 1106 8 0.3105662762529917 1106 9 0.3072639327150881 1106 10-11 0.4064294749566457 1106 12-13 0.3581653553644415 1106 14-15 0.486730461008527 1106 16-17 0.5277210692833734 1106 18-19 0.40896233441731056 1106 20-21 0.4180340491012444 1106 22-23 0.4885500467738524 1106 24-25 0.5727050189840384 1106 26-27 0.5404682404009762 1106 28-29 0.506819504090636 1106 30-31 0.5602938212922339 1106 32-33 0.631269497839277 1106 34-35 0.6983523558138245 1106 36-37 0.7355175581848741 1106 38-39 0.7045881829444056 1106 40-41 0.5317223332305119 1106 42-43 0.5581171126162303 1106 44-45 0.6158195031822444 1106 46-47 0.6746798236348468 1106 48-49 0.7327557149166566 1106 50-51 0.7633812054731877 1106 52-53 0.8047638925338632 1106 54-55 0.7627743034729662 1106 56-57 0.7319693087116619 1106 58-59 0.6435427255056041 1106 60-61 0.6372084644185989 1106 62-63 0.6923468095711769 1106 64-65 0.7308153370220865 1106 66-67 0.7234333000289865 1106 68-69 0.7731472334422449 1106 70-71 0.7490665723142911 1106 72-73 0.6244068716092315 1106 74-75 0.685957930116551 1106 76-77 0.6334638968852104 1106 78-79 0.4966504661374067 1106 80-81 0.505196274525602 1106 82-83 0.5669973128051708 1106 84-85 0.6141198805232904 1106 86-87 0.6183682442640688 1106 88-89 0.7090281232041349 1106 90-91 0.8838180503214907 1106 92-93 0.7125726291620076 1106 94-95 0.73058314461883 1106 96-97 0.677761908701406 1106 98-99 0.6858832305600657 1106 100 0.7218538569654314 1107 1 0.29995310713658085 1107 2 0.2680033418224639 1107 3 0.3307783909388853 1107 4 0.30488159412347926 1107 5 0.2628626730785939 1107 6 0.2893001098092185 1107 7 0.23871079706761833 1107 8 0.24774736365662875 1107 9 0.2618429288371118 1107 10-11 0.38333186218915216 1107 12-13 0.34447173911147644 1107 14-15 0.38628280585018615 1107 16-17 0.47295641696679525 1107 18-19 0.4073378427149308 1107 20-21 0.5085738258428023 1107 22-23 0.5422735721219993 1107 24-25 0.562592545870821 1107 26-27 0.4094093917048909 1107 28-29 0.35529186369059573 1107 30-31 0.36213134660898305 1107 32-33 0.403849981565898 1107 34-35 0.4869216452176772 1107 36-37 0.5449216528263605 1107 38-39 0.43361158426009894 1107 40-41 0.39934809273312766 1107 42-43 0.47921273929420494 1107 44-45 0.48191301031803846 1107 46-47 0.35052528545953265 1107 48-49 0.45869007976051535 1107 50-51 0.46029795849157296 1107 52-53 0.503978778785104 1107 54-55 0.515512045454031 1107 56-57 0.5136481234950807 1107 58-59 0.5068548241663748 1107 60-61 0.5139909405148728 1107 62-63 0.5716256353448088 1107 64-65 0.5553344168267742 1107 66-67 0.4521377887483169 1107 68-69 0.3928891006804065 1107 70-71 0.545088603777721 1107 72-73 0.5703188046316967 1107 74-75 0.4151927950153649 1107 76-77 0.4436770482519279 1107 78-79 0.36831758418805904 1107 80-81 0.47063252733202887 1107 82-83 0.4878652849528109 1107 84-85 0.4762755530461753 1107 86-87 0.44738153850362394 1107 88-89 0.38139645645896536 1107 90-91 0.32762663211226695 1107 92-93 0.4017133118115943 1107 94-95 0.4589697020113235 1107 96-97 0.41077711445399956 1107 98-99 0.40055507440476035 1107 100 0.6124334732497942 1108 1 0.2538072497629358 1108 2 0.3544764465912067 1108 3 0.22680426045021562 1108 4 0.2439474196998077 1108 5 0.2119322596279858 1108 6 0.25500655303989817 1108 7 0.25577418228527904 1108 8 0.2058379432738633 1108 9 0.23072087671344121 1108 10-11 0.1363531950267216 1108 12-13 0.11129365276296 1108 14-15 0.283040996651188 1108 16-17 0.24761750200752175 1108 18-19 0.26037402712227475 1108 20-21 0.3184066334946749 1108 22-23 0.32550871414147764 1108 24-25 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-0.6062194964019909 2316 56-57 -0.583101393508592 2316 58-59 -0.801037500582801 2316 60-61 -0.7119916551513299 2316 62-63 -0.7459149528130027 2316 64-65 -0.5135659706680116 2316 66-67 -0.39058104152238116 2316 68-69 -0.3446291488969706 2316 70-71 -0.4201336019787263 2316 72-73 -0.5490519554289008 2316 74-75 -0.38542371138434106 2316 76-77 -0.34757865988656533 2316 78-79 -0.5688400647175165 2316 80-81 -0.23996004879477795 2316 82-83 -0.17042510012699097 2316 84-85 -0.19144012128256804 2316 86-87 -0.10252831132842388 2316 88-89 -0.2019485401381047 2316 90-91 -0.22595302935120998 2316 92-93 0.011406993341370963 2316 94-95 -0.04274273953514296 2316 96-97 -0.18167963541976206 2316 98-99 -0.0127820735018922 2316 100 -0.21829140545226267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 5.0 1 28.0 2 49.0 3 51.0 4 66.0 5 54.0 6 188.0 7 1833.0 8 2928.0 9 2677.0 10 3353.0 11 5143.0 12 7525.0 13 9640.0 14 11054.0 15 12299.0 16 14299.0 17 17123.0 18 21215.0 19 24046.0 20 26805.0 21 30395.0 22 33705.0 23 34563.0 24 33954.0 25 33625.0 26 35817.0 27 39163.0 28 45278.0 29 52349.0 30 62245.0 31 75129.0 32 92256.0 33 118038.0 34 162974.0 35 222378.0 36 328616.0 37 526941.0 38 562240.0 39 141151.0 40 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.55799214204744 18.141901747962038 12.46804948163278 22.83205662835774 2 15.751703530928934 20.93281878648278 41.65215155391499 21.66332612867329 3 17.91692498450093 29.612684510605664 30.014979448053925 22.45541105683948 4 11.611784660560822 17.427747083704688 39.2926781107359 31.667790144998587 5 11.767511495636818 37.23285544738381 34.42441547123511 16.575217585744266 6 26.727781899654612 36.834938357540956 22.017426045349563 14.419853697454876 7 25.756549838963345 32.933016633886126 24.32681663316961 16.98361689398092 8 26.148546108793568 34.03037615879235 22.426438450195395 17.39463928221869 9 27.90026557965186 16.048384005319473 21.599045303310017 34.452305111718644 10-11 24.028213609756392 26.158152702150307 30.148154411045553 19.665479277047744 12-13 24.787854479754213 24.71347748116347 29.52254516942711 20.976122869655207 14-15 22.57001269660069 25.650196916447698 28.091197674445684 23.688592712505926 16-17 21.326050362061626 27.716402646418516 28.914610493777072 22.04293649774279 18-19 20.878464710278955 27.03733456207725 30.702085974332533 21.382114753311264 20-21 22.039513169205126 26.811453215076174 30.434287982909968 20.71474563280873 22-23 21.544948778579922 26.7404933707395 30.19088076828994 21.523677082390638 24-25 20.93254400131854 26.88417188663071 30.45024709173432 21.73303702031643 26-27 20.896480117630368 26.651221231937335 30.49858909482802 21.95370955560428 28-29 21.03236055127124 27.034626368587883 30.515915100115404 21.417097980025474 30-31 22.20614438806752 26.833335901168674 30.51978600735165 20.44073370341215 32-33 21.55365660688896 26.880743905655187 30.5163710269229 21.049228460532955 34-35 21.659482951680932 27.182588667132723 30.262365347894495 20.895563033291854 36-37 21.750236135377595 27.187405492100392 29.933545269910976 21.128813102611034 38-39 21.531819169632787 27.770940891861567 29.591280429392665 21.105959509112985 40-41 21.718719736890908 27.01591164287484 30.16357062089396 21.10179799934029 42-43 21.405951625593715 27.21506977274246 29.666581313910417 21.712397287753408 44-45 21.167693555970963 27.591348469557435 29.76220512173584 21.47875285273576 46-47 21.132017802150038 26.91635494151917 29.568815099256067 22.382812157074717 48-49 20.983011556727572 27.2267615903175 29.811896388948107 21.97833046400682 50-51 21.222242883569248 27.574675542208087 29.631954528794097 21.57112704542857 52-53 21.358733296091106 27.821878004356552 28.82141611952963 21.99797258002271 54-55 21.13347091312585 27.66253270327092 29.40173215801633 21.802264225586903 56-57 21.345345388386693 27.74646764422125 28.89120801058475 22.016978956807304 58-59 21.300308066526892 27.88025360572573 29.194526255889798 21.624912071857583 60-61 21.97525250163449 28.142149493007928 28.95908103756667 20.92351696779091 62-63 21.640271028946874 27.83840848005824 29.25007881144986 21.271241679545028 64-65 21.39988564595076 27.343411373730426 29.038402856306845 22.218300124011968 66-67 21.304789184990046 27.550139960469945 28.906692303392447 22.238378551147562 68-69 21.077463302339698 27.722496419194194 29.21410764025883 21.985932638207277 70-71 21.23297564003637 27.363770176471665 29.361176807482494 22.042077376009473 72-73 20.93841053669026 28.071767762745274 28.733948570916585 22.25587312964788 74-75 21.30834824416551 27.80729679529854 28.71907812977397 22.16527683076198 76-77 20.998608420391417 27.433736927850173 29.311446696337697 22.25620795542072 78-79 20.9749184850765 27.50060912250529 29.15022035902397 22.374252033394246 80-81 21.039257428066392 27.871870938567035 28.572457486975644 22.516414146390932 82-83 21.464279917286937 28.015395293123646 28.576514062188156 21.943810727401264 84-85 21.230271664419114 28.36204269398772 28.12646630580427 22.281219335788887 86-87 20.883138252309806 28.10865198717125 28.706857460332984 22.301352300185954 88-89 20.78854202441936 27.71769772000346 28.99656897812305 22.49719127745413 90-91 21.655542649055295 27.81450546095467 28.734599770979795 21.795352119010236 92-93 21.228581032123884 27.89821237754575 28.714191828810225 22.159014761520144 94-95 20.940182118451883 27.594657629421594 28.7609273400193 22.704232912107226 96-97 21.08120464436019 27.684219049959907 28.74710049953457 22.48747580614533 98-99 21.141983271130375 27.458002253065715 28.639715156310018 22.76029931949389 100 21.049909629583436 27.60990945755171 28.637440626654236 22.702740286210616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1368.0 1 992.5 2 2329.5 3 3525.5 4 2123.5 5 1342.5 6 1724.0 7 3016.0 8 4707.0 9 5446.0 10 4740.5 11 3803.5 12 3740.5 13 3927.0 14 4200.0 15 4542.0 16 4819.0 17 5096.0 18 5469.5 19 5880.5 20 6379.5 21 7074.0 22 8173.0 23 9794.0 24 11673.5 25 13935.5 26 17101.0 27 21081.5 28 25282.0 29 30454.0 30 36789.5 31 42660.5 32 49825.0 33 58603.5 34 67026.0 35 74466.0 36 83378.0 37 91364.5 38 95444.5 39 98423.0 40 96967.5 41 94373.0 42 92917.0 43 91607.5 44 92571.5 45 94611.0 46 95306.0 47 94473.0 48 95979.0 49 100371.0 50 106799.5 51 105152.0 52 94301.0 53 86324.0 54 80772.0 55 73892.0 56 66731.0 57 60770.0 58 54535.0 59 46424.0 60 35665.0 61 25841.0 62 19877.0 63 15127.5 64 11060.5 65 8161.0 66 5986.5 67 4618.5 68 3680.0 69 2805.0 70 2220.5 71 1829.5 72 1525.0 73 1276.5 74 1025.0 75 801.5 76 667.5 77 560.0 78 480.0 79 418.5 80 339.5 81 286.0 82 247.0 83 208.0 84 176.0 85 151.0 86 121.5 87 87.0 88 66.0 89 51.5 90 36.5 91 24.5 92 19.5 93 16.5 94 9.5 95 8.5 96 7.0 97 2.5 98 3.5 99 5.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16808631296654072 2 0.10568086599558718 3 0.033423812872502665 4 0.03517918996870055 5 0.02862339387473701 6 0.0030092178791963813 7 0.00476459497539427 8 0.0055527234675647515 9 0.006090083803135533 10-11 0.005230307266222281 12-13 0.008508205313204054 14-15 0.018574755599563376 16-17 0.009117213693517607 18-19 0.01780453911857859 20-21 0.019918156438490334 22-23 0.04766386176512839 24-25 0.016317842190166094 26-27 0.010997974868015346 28-29 0.02932196231097902 30-31 0.01741047487249335 32-33 0.022891550295315328 34-35 0.022210893870259006 36-37 0.023321438563771956 38-39 0.021404853366902832 40-41 0.028426361751694388 42-43 0.03734654332216937 44-45 0.040087081033580364 46-47 0.022801990239386866 48-49 0.01461620112752528 50-51 0.013684776545869259 52-53 0.024503631302027675 54-55 0.028659217897108395 56-57 0.022049685769587768 58-59 0.02837262571813731 60-61 0.02856965784117993 62-63 0.055043610373633804 64-65 0.03462391762194408 66-67 0.022748254205829786 68-69 0.030450419015677663 70-71 0.04406354751680415 72-73 0.04202157824163518 74-75 0.04390233941613292 76-77 0.025417143872498004 78-79 0.01873596370023461 80-81 0.024718575436255986 82-83 0.020419692751689728 84-85 0.02179891761298807 86-87 0.023625942753928732 88-89 0.020186836606275724 90-91 0.030074266780778118 92-93 0.02826515365102315 94-95 0.021566061467574064 96-97 0.015225209507838832 98-99 0.02002562850560449 100 0.04442178774051801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2791423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.21406602014378 #Duplication Level Percentage of deduplicated Percentage of total 1 81.31281551902539 53.8405213506052 2 12.997828244235416 17.212781149645867 3 2.9555963090610935 5.8710614741119365 4 0.9983258257477288 2.6441284854269864 5 0.4736082352628011 1.5679763478687454 6 0.27680212281304295 1.0996916420675267 7 0.1832108208561375 0.8491793371441116 8 0.12963453957743842 0.686690396965716 9 0.08646107812040227 0.5152445581353412 >10 0.5064320943542964 6.115733323281474 >50 0.04214007621287404 1.941734950732682 >100 0.0337456219550636 4.182687261980065 >500 0.002348754283257321 1.014075977003434 >1k 8.035212021637207E-4 0.9483889989113052 >5k 1.2361864648672625E-4 0.5509111601581617 >10k+ 1.2361864648672625E-4 0.959193585961432 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12676 0.45410530757968254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10761 0.38550230473847924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6951 0.24901277950350054 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6510 0.23321438563771954 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3537 0.1267095671275905 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3463 0.12405858947210796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.5824022371385494E-5 7 0.0 0.0 0.0 0.0 3.5824022371385494E-5 8 0.0 0.0 0.0 0.0 3.5824022371385494E-5 9 0.0 0.0 0.0 0.0 3.5824022371385494E-5 10-11 0.0 0.0 0.0 0.0 3.5824022371385494E-5 12-13 0.0 0.0 0.0 0.0 3.5824022371385494E-5 14-15 0.0 0.0 0.0 0.0 5.373603355707824E-5 16-17 0.0 0.0 0.0 0.0 7.164804474277099E-5 18-19 0.0 0.0 0.0 7.164804474277099E-5 7.164804474277099E-5 20-21 0.0 0.0 0.0 1.0747206711415647E-4 7.164804474277099E-5 22-23 0.0 0.0 0.0 2.1494413422831296E-4 8.956005592846373E-5 24-25 0.0 0.0 0.0 3.582402237138549E-4 1.0747206711415647E-4 26-27 0.0 0.0 0.0 4.657122908280114E-4 1.0747206711415647E-4 28-29 0.0 0.0 0.0 0.001415048883669727 1.0747206711415647E-4 30-31 0.0 0.0 0.0 0.005051187154365354 1.0747206711415647E-4 32-33 0.0 0.0 0.0 0.013792248612983414 1.0747206711415647E-4 34-35 0.0 0.0 0.0 0.025614175995540625 1.0747206711415647E-4 36-37 3.5824022371385494E-5 0.0 0.0 0.04461881986356063 1.0747206711415647E-4 38-39 3.5824022371385494E-5 0.0 0.0 0.08189371514098723 1.0747206711415647E-4 40-41 3.5824022371385494E-5 0.0 0.0 0.13297877104258293 1.0747206711415647E-4 42-43 3.5824022371385494E-5 0.0 0.0 0.1832756984520082 1.0747206711415647E-4 44-45 3.5824022371385494E-5 0.0 0.0 0.23953732558626908 1.0747206711415647E-4 46-47 3.5824022371385494E-5 0.0 0.0 0.300169483449839 1.0747206711415647E-4 48-49 3.5824022371385494E-5 0.0 0.0 0.37321466506509404 2.5076815659969844E-4 50-51 5.373603355707824E-5 0.0 0.0 0.4548755240606673 2.5076815659969844E-4 52-53 7.164804474277099E-5 1.7912011185692747E-5 0.0 0.54285932300479 2.5076815659969844E-4 54-55 7.164804474277099E-5 3.5824022371385494E-5 0.0 0.6578902588393089 2.5076815659969844E-4 56-57 7.164804474277099E-5 7.164804474277099E-5 0.0 0.7794949027789768 2.5076815659969844E-4 58-59 7.164804474277099E-5 7.164804474277099E-5 0.0 0.9104137925352052 2.5076815659969844E-4 60-61 7.164804474277099E-5 7.164804474277099E-5 0.0 1.0515246166560925 2.5076815659969844E-4 62-63 7.164804474277099E-5 7.164804474277099E-5 0.0 1.2090965790566317 2.5076815659969844E-4 64-65 7.164804474277099E-5 7.164804474277099E-5 0.0 1.366077445088043 2.5076815659969844E-4 66-67 7.164804474277099E-5 7.164804474277099E-5 0.0 1.5458782133700266 2.5076815659969844E-4 68-69 7.164804474277099E-5 7.164804474277099E-5 0.0 1.7618254202247385 2.5076815659969844E-4 70-71 8.956005592846373E-5 7.164804474277099E-5 0.0 2.0062527248647015 2.5076815659969844E-4 72-73 1.4329608948554197E-4 7.164804474277099E-5 0.0 2.2362966845225536 3.582402237138549E-4 74-75 1.4329608948554197E-4 7.164804474277099E-5 0.0 2.4785387237978624 3.7615223489954766E-4 76-77 1.4329608948554197E-4 7.164804474277099E-5 0.0 2.742113968395331 3.940642460852404E-4 78-79 1.4329608948554197E-4 7.164804474277099E-5 0.0 3.0496094644201186 3.940642460852404E-4 80-81 1.4329608948554197E-4 8.956005592846373E-5 0.0 3.353415086140653 4.478002796423187E-4 82-83 1.4329608948554197E-4 1.0747206711415647E-4 0.0 3.6837125724048274 5.015363131993969E-4 84-85 1.4329608948554197E-4 1.0747206711415647E-4 0.0 4.031671301698094 5.015363131993969E-4 86-87 1.4329608948554197E-4 1.0747206711415647E-4 0.0 4.399691483519337 5.015363131993969E-4 88 1.4329608948554197E-4 1.0747206711415647E-4 0.0 4.6932693468528415 5.015363131993969E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5430 0.0 65.0323 2 GTACCTG 6020 0.0 60.148766 1 TATAACG 475 0.0 59.39456 2 ACCTGGG 5930 0.0 58.646255 3 TAACGCA 535 0.0 50.946472 4 GTACCCG 505 0.0 43.823128 1 CCTGGGG 4005 0.0 39.777462 4 ATAACGC 740 0.0 39.37524 3 TACCCGG 510 0.0 38.722923 2 TATCACG 305 0.0 38.54155 2 GTATAAC 1140 0.0 33.86927 1 GGTATCA 10100 0.0 29.230955 1 GTATCAA 14790 0.0 28.525724 1 CCTGGGA 4520 0.0 28.48735 4 TCACGCA 450 0.0 27.151932 4 CTGGGGG 2990 0.0 25.930204 5 CATGGGG 6515 0.0 25.534582 4 GTACACG 480 0.0 25.505215 1 ATCACGC 480 0.0 25.456305 3 TGGGGGG 5775 0.0 24.810307 6 >>END_MODULE