FastQCFastQC Report
Mon 6 Jun 2016
ERR1211511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791423
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139610.5001391763269128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119120.426735754487944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.28917150858182367No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62250.2230045392618747No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45440.16278435765557567No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.12258980455488115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG53500.064.243942
ACCTGGG55650.059.3600353
GTACCTG59250.058.567941
TAACGCA5250.054.597724
TATAACG5500.051.292892
TATCACG2850.044.5438232
CCTGGGG37950.043.0894974
GTACCCG5500.040.1815871
ATAACGC8200.036.101953
TACCCGG6000.033.6965752
GTATAAC11050.031.9147111
CTGGGGG26400.030.258615
ATCACGC4200.030.2077563
GTATCAA158150.027.5317441
CCTGGGA40350.027.4835244
TGGGGGG46400.027.2414686
TCACGCA5050.026.9842684
ACAACGC3850.026.851343
GGTATCA109600.026.513671
ATGGGGG40450.026.486245
Produced by FastQC (version 0.11.5)