##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2791423 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13945288836554 33.0 31.0 34.0 30.0 34.0 2 32.336540538642836 34.0 31.0 34.0 31.0 34.0 3 32.34519741364888 34.0 31.0 34.0 30.0 34.0 4 35.98860903560657 37.0 35.0 37.0 35.0 37.0 5 35.9279632646145 37.0 35.0 37.0 35.0 37.0 6 35.94934877300932 37.0 35.0 37.0 35.0 37.0 7 35.887149314167004 37.0 35.0 37.0 35.0 37.0 8 35.87705661234431 37.0 35.0 37.0 35.0 37.0 9 37.522532056230816 39.0 37.0 39.0 35.0 39.0 10-11 37.4736688061967 39.0 37.0 39.0 34.5 39.0 12-13 37.39159919510587 39.0 37.0 39.0 34.0 39.0 14-15 38.609563294420084 40.0 38.0 41.0 34.0 41.0 16-17 38.53705511490018 40.0 38.0 41.0 34.0 41.0 18-19 38.54723558557768 40.0 38.0 41.0 34.0 41.0 20-21 38.49017078386185 40.0 38.0 41.0 34.0 41.0 22-23 38.362968457306536 40.0 38.0 41.0 34.0 41.0 24-25 38.2583759251106 40.0 38.0 41.0 34.0 41.0 26-27 38.07894074097691 40.0 37.0 41.0 33.0 41.0 28-29 38.09749973400663 40.0 37.0 41.0 33.0 41.0 30-31 37.98427325417896 40.0 37.0 41.0 33.0 41.0 32-33 37.73506326343231 40.0 37.0 41.0 33.0 41.0 34-35 37.57022995081719 40.0 37.0 41.0 32.5 41.0 36-37 37.485105087978425 40.0 37.0 41.0 32.0 41.0 38-39 37.38369910257242 40.0 36.0 41.0 32.0 41.0 40-41 37.21690084233023 40.0 36.0 41.0 31.0 41.0 42-43 37.06555939390053 40.0 36.0 41.0 31.0 41.0 44-45 36.87584450654738 39.5 36.0 41.0 30.5 41.0 46-47 36.58837893791088 39.0 35.0 41.0 30.0 41.0 48-49 36.352506588933316 39.0 35.0 41.0 29.0 41.0 50-51 36.14758780736564 39.0 35.0 41.0 28.0 41.0 52-53 35.97047115395982 39.0 35.0 40.0 27.5 41.0 54-55 35.784612901735066 39.0 35.0 40.0 27.0 41.0 56-57 35.59169122701934 38.0 34.5 40.0 27.0 41.0 58-59 35.350352490468126 38.0 34.0 40.0 26.0 41.0 60-61 35.03251979366796 38.0 34.0 40.0 26.0 41.0 62-63 34.69147169741025 37.5 33.5 40.0 25.5 41.0 64-65 34.397223924858395 37.0 33.0 40.0 25.0 41.0 66-67 34.072107308709576 36.5 33.0 39.0 24.5 41.0 68-69 33.683588800407534 36.0 32.0 39.0 23.0 40.0 70-71 33.29503984168648 35.5 32.0 39.0 22.5 40.0 72-73 32.882783619680716 35.0 31.5 38.0 22.0 40.0 74-75 32.46651098740678 35.0 31.0 37.0 21.5 39.0 76-77 30.917410224104337 33.5 29.5 35.5 20.0 38.5 78-79 31.73745792020772 34.0 31.0 36.0 20.5 39.0 80-81 31.704234542740387 34.5 31.0 36.0 20.0 37.5 82-83 31.41672634351727 34.0 31.0 35.5 20.0 37.0 84-85 31.100545134148426 34.0 30.0 35.0 20.0 37.0 86-87 30.808136746025234 34.0 30.0 35.0 19.0 36.0 88-89 30.545471968956335 34.0 30.0 35.0 18.5 36.0 90-91 30.247875904153545 34.0 29.0 35.0 18.0 36.0 92-93 30.02574403807664 34.0 29.0 35.0 18.0 35.0 94-95 29.816557719843964 34.0 29.0 35.0 17.0 35.0 96-97 29.621483021383717 34.0 29.0 35.0 15.5 35.0 98-99 29.44614915045122 34.0 29.0 35.0 15.0 35.0 100 27.645436037461895 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3338710830473204 1101 2 0.26424904063930654 1101 3 0.3130305780849838 1101 4 0.16424865994756033 1101 5 0.19345691538974563 1101 6 0.24381565505068892 1101 7 0.26832123572503264 1101 8 0.21674110247963085 1101 9 0.26707738782601353 1101 10-11 0.24458569855894297 1101 12-13 0.282440155140371 1101 14-15 0.3830605387190076 1101 16-17 0.3694492409369303 1101 18-19 0.3712378482616714 1101 20-21 0.3538029424705016 1101 22-23 0.38780061722491865 1101 24-25 0.28974520899446077 1101 26-27 0.2307898691774497 1101 28-29 0.4372683603467138 1101 30-31 0.45192089954365855 1101 32-33 0.5067976017385902 1101 34-35 0.4614472043287279 1101 36-37 0.5429719951552485 1101 38-39 0.6136727378426556 1101 40-41 0.5850632527931694 1101 42-43 0.6048627677047236 1101 44-45 0.6966716824529229 1101 46-47 0.7207964196212302 1101 48-49 0.7636518637501055 1101 50-51 0.6645706859015732 1101 52-53 0.6098994632618897 1101 54-55 0.5359344058332098 1101 56-57 0.6806569803834535 1101 58-59 0.7038350054536266 1101 60-61 0.5947573395415446 1101 62-63 0.7010204705749032 1101 64-65 0.5430436145776198 1101 66-67 0.4725384727173463 1101 68-69 0.4301746364431338 1101 70-71 0.49140085194056127 1101 72-73 0.37123659093976613 1101 74-75 0.5617202617715762 1101 76-77 0.6099873236035585 1101 78-79 0.7014711743358077 1101 80-81 0.574263766324556 1101 82-83 0.2983803551322879 1101 84-85 0.4543959363446639 1101 86-87 0.5626233536994967 1101 88-89 0.4951003139696226 1101 90-91 0.393274150688093 1101 92-93 0.315215002341116 1101 94-95 0.6273093358951094 1101 96-97 0.46546189580277186 1101 98-99 0.4381907350048593 1101 100 0.4729587564756734 1102 1 0.2791037669618177 1102 2 0.22958105731723322 1102 3 0.299251677598356 1102 4 0.146703386654238 1102 5 0.152330556041413 1102 6 0.13760692626202342 1102 7 0.15346880569335042 1102 8 0.13846974103893217 1102 9 0.16646190226953905 1102 10-11 0.1870787400262941 1102 12-13 0.2113725965258837 1102 14-15 0.31312582104055764 1102 16-17 0.27876471055471086 1102 18-19 0.19415559952211936 1102 20-21 0.32465138274450567 1102 22-23 0.2170216683253372 1102 24-25 0.20676734609242686 1102 26-27 0.24237935476885042 1102 28-29 0.32548932368484174 1102 30-31 0.3762979365074912 1102 32-33 0.33599604907556824 1102 34-35 0.2795913612945711 1102 36-37 0.4074350767434467 1102 38-39 0.4162629633342618 1102 40-41 0.45939019672235304 1102 42-43 0.28427021971787525 1102 44-45 0.3527608931516042 1102 46-47 0.4337091704207694 1102 48-49 0.4726552687942913 1102 50-51 0.3837058272264926 1102 52-53 0.4152777441511759 1102 54-55 0.39712574464582673 1102 56-57 0.28880095607706124 1102 58-59 0.4293135144143321 1102 60-61 0.2567717206195539 1102 62-63 0.4385421983734261 1102 64-65 0.3731466545793225 1102 66-67 0.38977962867713245 1102 68-69 0.5421875585007214 1102 70-71 0.3260240919695363 1102 72-73 0.28481571615975554 1102 74-75 0.28407392478604976 1102 76-77 0.30350228729495043 1102 78-79 0.43953832155074224 1102 80-81 0.4078412092497814 1102 82-83 0.31488253935218324 1102 84-85 0.2524714884015147 1102 86-87 0.24772717516137632 1102 88-89 0.2775952411103404 1102 90-91 0.3208105195355273 1102 92-93 0.2651256704995184 1102 94-95 0.18953705449781566 1102 96-97 0.29995128176076236 1102 98-99 0.2357562378374567 1102 100 0.2514429801541169 1103 1 0.3457718344033722 1103 2 0.2866066028648362 1103 3 0.3780611568908654 1103 4 0.16517804306354122 1103 5 0.17110513470770883 1103 6 0.2379751842907325 1103 7 0.2650400986110597 1103 8 0.2918364590329716 1103 9 0.2920575471253244 1103 10-11 0.2923181216104922 1103 12-13 0.30184379337674017 1103 14-15 0.4779027570616634 1103 16-17 0.48205280561428054 1103 18-19 0.5039173223813691 1103 20-21 0.41052455381336017 1103 22-23 0.4092773279447215 1103 24-25 0.3609675429190702 1103 26-27 0.4675059391332397 1103 28-29 0.46164240263959044 1103 30-31 0.5649245581488529 1103 32-33 0.5835159042596274 1103 34-35 0.5030527478505249 1103 36-37 0.5732057985502976 1103 38-39 0.5271080220244215 1103 40-41 0.6537149679436425 1103 42-43 0.7168110166642165 1103 44-45 0.6380026916057702 1103 46-47 0.6436093673434087 1103 48-49 0.6522490255904145 1103 50-51 0.6994823138974979 1103 52-53 0.6817249122376552 1103 54-55 0.7088145639984518 1103 56-57 0.7576508512802533 1103 58-59 0.8130352979107798 1103 60-61 0.7647028084680798 1103 62-63 0.8233576528596416 1103 64-65 0.8344864410052324 1103 66-67 0.8584649506077255 1103 68-69 0.8303465595334885 1103 70-71 0.8664213375057201 1103 72-73 0.6750763808117952 1103 74-75 0.60126496299808 1103 76-77 0.5155475896346111 1103 78-79 0.6179262031556902 1103 80-81 0.6149622190278592 1103 82-83 0.7293507271679402 1103 84-85 0.7189782676364445 1103 86-87 0.6725665189033201 1103 88-89 0.7047432502910347 1103 90-91 0.6354678439580859 1103 92-93 0.5932269140996418 1103 94-95 0.5962876304242783 1103 96-97 0.5334218270379836 1103 98-99 0.5955445025365549 1103 100 0.7539691212265822 1104 1 0.27110797073707715 1104 2 0.2229719026713468 1104 3 0.26050281897074967 1104 4 0.10050308670828656 1104 5 0.13415472510552462 1104 6 0.18702342060383614 1104 7 0.20303395120154732 1104 8 0.21178833584165346 1104 9 0.27154789125194156 1104 10-11 0.28159365885458243 1104 12-13 0.23774747819241782 1104 14-15 0.31590697211462526 1104 16-17 0.2859047397436285 1104 18-19 0.22695049112531507 1104 20-21 0.09433680456552906 1104 22-23 0.23581345220647876 1104 24-25 0.22684868146455983 1104 26-27 0.2706801450013856 1104 28-29 0.33674207011218016 1104 30-31 0.249303314805303 1104 32-33 0.2979290765322915 1104 34-35 0.3464672341087507 1104 36-37 0.2171924784595305 1104 38-39 0.3861668719472604 1104 40-41 0.38319771433808825 1104 42-43 0.3886507188668844 1104 44-45 0.3237781119291938 1104 46-47 0.30384564415074067 1104 48-49 0.3853057662983801 1104 50-51 0.3719348998278491 1104 52-53 0.4456366763972923 1104 54-55 0.5584796095341957 1104 56-57 0.5899776082136086 1104 58-59 0.44311137442500836 1104 60-61 0.3836859322165722 1104 62-63 0.4328099492319808 1104 64-65 0.5786516992774793 1104 66-67 0.44281017171861947 1104 68-69 0.48555398184245746 1104 70-71 0.2913021902920576 1104 72-73 0.4186954465608821 1104 74-75 0.4618968072884755 1104 76-77 0.4469936216767785 1104 78-79 0.5425612904114203 1104 80-81 0.5833005696783999 1104 82-83 0.3918485428199432 1104 84-85 0.36282924360734725 1104 86-87 0.4272063589626178 1104 88-89 0.4891818179834502 1104 90-91 0.4449709079546267 1104 92-93 0.2962079265227757 1104 94-95 0.29483642137182997 1104 96-97 0.44319970733824476 1104 98-99 0.5062529467404957 1104 100 0.4319447308179569 1105 1 0.24181459007692752 1105 2 0.16295955617967195 1105 3 0.20493622218033636 1105 4 0.15104838305784796 1105 5 0.12654323535741696 1105 6 0.13542787074189988 1105 7 0.17527375500794307 1105 8 0.12544285518083598 1105 9 0.17751370252531018 1105 10-11 0.1943284151991378 1105 12-13 0.2292552565665673 1105 14-15 0.297128363360855 1105 16-17 0.3057212597376946 1105 18-19 0.33428954367765584 1105 20-21 0.24208941395392714 1105 22-23 0.39445310596460814 1105 24-25 0.365678144598931 1105 26-27 0.33520469958249777 1105 28-29 0.354058066878288 1105 30-31 0.4917277969784237 1105 32-33 0.392188801820744 1105 34-35 0.4070902593452175 1105 36-37 0.5042601191740914 1105 38-39 0.4752378849503245 1105 40-41 0.5208151030593271 1105 42-43 0.5048607003682477 1105 44-45 0.6506004220312107 1105 46-47 0.5736264032025815 1105 48-49 0.4503820309909585 1105 50-51 0.5652821830933306 1105 52-53 0.6330499640241314 1105 54-55 0.5979818425360364 1105 56-57 0.6056036631086599 1105 58-59 0.5703497562328792 1105 60-61 0.5696151934862215 1105 62-63 0.5946363354484632 1105 64-65 0.7473097968206659 1105 66-67 0.6016099585454242 1105 68-69 0.7771297165557556 1105 70-71 0.7440670491217887 1105 72-73 0.6819345904249374 1105 74-75 0.7369687089351231 1105 76-77 0.6634102993143536 1105 78-79 0.7180075321157204 1105 80-81 0.7045793218978957 1105 82-83 0.6567804767717007 1105 84-85 0.6602639709211964 1105 86-87 0.6809220157993501 1105 88-89 0.5930087029543216 1105 90-91 0.7085266258233673 1105 92-93 0.7758999309246413 1105 94-95 0.747271203540226 1105 96-97 0.8220144188202312 1105 98-99 0.7517256536518317 1105 100 0.8127761906576794 1106 1 0.15576474025859 1106 2 0.1334122776190867 1106 3 0.16984190602347837 1106 4 0.10485480967999905 1106 5 0.1248937309212721 1106 6 0.08089965232976937 1106 7 0.0971958082312625 1106 8 0.10532848781258508 1106 9 0.13720691348503777 1106 10-11 0.12143492596447913 1106 12-13 0.20778109254111143 1106 14-15 0.24107452338059687 1106 16-17 0.2975262055952044 1106 18-19 0.2770346066619709 1106 20-21 0.282934162324338 1106 22-23 0.29188975080845125 1106 24-25 0.16537784604932426 1106 26-27 0.26933901257832105 1106 28-29 0.29011296917438045 1106 30-31 0.24753523150511825 1106 32-33 0.3290679705197377 1106 34-35 0.33189837854742166 1106 36-37 0.32417912406916827 1106 38-39 0.4004110132826071 1106 40-41 0.34346892347742397 1106 42-43 0.3555928562151607 1106 44-45 0.4431562114258014 1106 46-47 0.4703681704167977 1106 48-49 0.4608662462083757 1106 50-51 0.4854842886810431 1106 52-53 0.48359297583203187 1106 54-55 0.4200452355857678 1106 56-57 0.3773482642446879 1106 58-59 0.4634582737387447 1106 60-61 0.4831174220823371 1106 62-63 0.6961891950537051 1106 64-65 0.549867063093366 1106 66-67 0.5822377548165676 1106 68-69 0.4918977527677768 1106 70-71 0.6230649031142477 1106 72-73 0.5236093702049232 1106 74-75 0.5233603135308087 1106 76-77 0.6075507854015818 1106 78-79 0.7034957472113632 1106 80-81 0.7162439060368726 1106 82-83 0.6507456807460557 1106 84-85 0.5833927890235806 1106 86-87 0.5656249592562936 1106 88-89 0.6235517406723616 1106 90-91 0.4869579869414409 1106 92-93 0.645100350409848 1106 94-95 0.7497407639811406 1106 96-97 0.6207164178524849 1106 98-99 0.6200929001227102 1106 100 0.7658597243088039 1107 1 0.1771466032576683 1107 2 0.1281821110387682 1107 3 0.17369044510940768 1107 4 0.1514238989048735 1107 5 0.12503012738556407 1107 6 0.10411452680576616 1107 7 0.1232563643363278 1107 8 0.090056008890258 1107 9 0.11503709105556936 1107 10-11 0.17014759847166516 1107 12-13 0.16456745446470933 1107 14-15 0.19994804558869106 1107 16-17 0.1283754551202776 1107 18-19 0.154960261757509 1107 20-21 0.22257038608519508 1107 22-23 0.27383488214156415 1107 24-25 0.3081569779790385 1107 26-27 0.19762881679542232 1107 28-29 0.29855188710885017 1107 30-31 0.21497229287763986 1107 32-33 0.2764974368661157 1107 34-35 0.34333507488767623 1107 36-37 0.41306791483110317 1107 38-39 0.361410455921515 1107 40-41 0.28347999219769093 1107 42-43 0.3143942551129939 1107 44-45 0.3355124161892675 1107 46-47 0.2240032833805472 1107 48-49 0.30917767760585946 1107 50-51 0.27192569511503706 1107 52-53 0.3437439147107213 1107 54-55 0.3052136410620392 1107 56-57 0.30649716993291776 1107 58-59 0.479102653811907 1107 60-61 0.39420998719938183 1107 62-63 0.29059713627174233 1107 64-65 0.4501897045139387 1107 66-67 0.3021221023900864 1107 68-69 0.2788263595220499 1107 70-71 0.39814418969204723 1107 72-73 0.5084413964872496 1107 74-75 0.5143807160868121 1107 76-77 0.4616095124202495 1107 78-79 0.48918844499604575 1107 80-81 0.5536102571272608 1107 82-83 0.6262610435017706 1107 84-85 0.39625684001819295 1107 86-87 0.2959088012797473 1107 88-89 0.33201901729616523 1107 90-91 0.32477866582295434 1107 92-93 0.40701050929700955 1107 94-95 0.5084863286428742 1107 96-97 0.40445638927791805 1107 98-99 0.3960273630935234 1107 100 0.6219231849716707 1108 1 0.17698740297730353 1108 2 0.09986689875748311 1108 3 0.18181265109106448 1108 4 0.11206552945765225 1108 5 0.14562348499426747 1108 6 0.11534551893336698 1108 7 0.14512108042560357 1108 8 0.12447788749630462 1108 9 0.12588311767300553 1108 10-11 0.11640740691846219 1108 12-13 0.12242975797524025 1108 14-15 0.12812248849455443 1108 16-17 0.16389618962121943 1108 18-19 0.2333790610668558 1108 20-21 0.18828270583628637 1108 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-0.26135767017662204 2316 80-81 -0.25094088559815475 2316 82-83 0.009902835857253933 2316 84-85 -0.046766058398898025 2316 86-87 -0.1062535678172658 2316 88-89 -0.07630567150051348 2316 90-91 -0.08775646118312608 2316 92-93 0.10819014551295325 2316 94-95 0.15378521006727297 2316 96-97 0.0691252074737072 2316 98-99 0.09784916488938933 2316 100 -0.14993239912458023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 4.0 11 16.0 12 36.0 13 114.0 14 342.0 15 1083.0 16 2589.0 17 5768.0 18 10545.0 19 14949.0 20 18694.0 21 23307.0 22 28007.0 23 29931.0 24 30350.0 25 31582.0 26 35382.0 27 42006.0 28 50227.0 29 60485.0 30 73067.0 31 88328.0 32 107842.0 33 135060.0 34 181023.0 35 239905.0 36 344689.0 37 553637.0 38 583519.0 39 98911.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23763254491247 19.53080945990389 13.476501065088248 22.75505693009539 2 15.143237442593355 21.87412283579056 41.208462073704915 21.774177647911173 3 16.98541273271866 30.37489696056505 30.65239142840868 21.987298878307605 4 11.3158785629459 18.312140302105927 39.1814874588245 31.19049367612367 5 11.310973217801013 37.90240559381436 34.45502905563542 16.331592132749197 6 26.657705820334794 37.63329766429283 21.668171883457887 14.04082463191449 7 25.578459445236355 33.282236336090946 24.287254206904507 16.852050011768192 8 26.1981144275987 34.687238371187 22.1102476278092 17.004399573405095 9 27.803678555230988 16.146931563912588 21.617906572349145 34.431483308507275 10-11 24.05669079892227 26.572199913807403 30.050963254225532 19.320146033044793 12-13 24.864719535520056 24.94412348110623 29.557953058350527 20.633203925023185 14-15 22.566339820227892 25.923068628437896 28.10518147912373 23.40541007221048 16-17 21.338326279835115 27.993990122176847 28.934341117527033 21.733342480461 18-19 20.943073941935083 27.229146881145006 30.879259925062502 20.948519251857405 20-21 22.027171659362793 27.02549235086173 30.55383146549446 20.39350452428101 22-23 21.515585420052783 26.92309621293512 30.35956929494383 21.20174907206826 24-25 20.976079400377408 27.0377379275262 30.57326506467581 21.41291760742059 26-27 20.98211750839851 26.769911702566883 30.669203012447976 21.578767776586627 28-29 21.119430936147797 27.202329017624365 30.703127812777247 20.975112233450595 30-31 22.15229665889393 26.93561000809277 30.765900462889157 20.146192870124143 32-33 21.615979161229454 26.99831317303133 30.763930025609604 20.621777640129608 34-35 21.682613000192205 27.320347817738 30.46326796271043 20.533771219359366 36-37 21.76112818243941 27.328671980024303 30.179059376279042 20.73114046125724 38-39 21.689493177482188 27.8077897987072 29.79950966931607 20.70320735449454 40-41 21.787309307506177 27.17676587823338 30.34128860154897 20.69463621271147 42-43 21.475697594829207 27.372984995246696 29.803037422226986 21.348279987697115 44-45 21.281725356389103 27.70606188385823 29.896702601440712 21.11551015831196 46-47 21.20197570618706 27.116357830463517 29.681110789266786 22.00055567408264 48-49 21.024033946969325 27.392243487245704 29.924472722439315 21.659249843345656 50-51 21.24417544007985 27.81868215726724 29.70047270025272 21.23666970240019 52-53 21.348463342406706 27.95143727801705 28.943764200281013 21.75633517929523 54-55 21.156252961144588 27.786279204610366 29.5113871850708 21.546080649174247 56-57 21.443070353307014 27.85316371348528 28.94471830733626 21.759047625871446 58-59 21.361534724690184 28.009248644372857 29.30207413818799 21.327142492748973 60-61 22.05860909535487 28.280939594189608 29.014601220619767 20.64585008983575 62-63 21.771659630072254 28.061097715894046 29.287842423758185 20.879400230275515 64-65 21.4827243640152 27.56497776484872 29.130197710673265 21.82210016046281 66-67 21.36554394914317 27.70330076049857 29.031042809013343 21.90011248134492 68-69 21.119418974269312 27.83823660080887 29.282614085524372 21.75973033939745 70-71 21.25839141372706 27.635256799293217 29.361009384339464 21.745342402640254 72-73 21.021098269364742 28.21015545103263 28.71569061412822 22.053055665474407 74-75 21.38589351350964 27.960889751875445 28.6447727614694 22.008443973145514 76-77 20.98984319666854 27.649479764313156 29.26437244454348 22.096304594474823 78-79 20.942811570962107 27.718334877475375 29.137996140719707 22.200857410842815 80-81 20.972021719982898 28.16276695657813 28.556335511505672 22.30887581193331 82-83 21.38608685495045 28.30758145406064 28.468656852252423 21.837674838736493 84-85 21.169570653528275 28.63539400936148 28.09825631797863 22.09677901913161 86-87 20.804622413329753 28.374003404102837 28.588408134014152 22.23296604855326 88-89 20.758909695497316 28.056958986376568 28.787005783329388 22.397125534796725 90-91 21.644340123118035 28.12325706851937 28.57251123493744 21.659891573425153 92-93 21.15076178887297 28.306214238371496 28.50385342662094 22.039170546134596 94-95 20.776840604988628 28.03988293294089 28.480165598318358 22.703110863752126 96-97 20.87291293893207 28.129527814610945 28.524763982554425 22.472795263902555 98-99 20.920446621993 27.804913529871662 28.456476140722764 22.818163707412577 100 19.89463549708103 28.634414269605713 28.619006959446168 22.851943273867086 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1419.0 1 1104.0 2 2297.0 3 3465.5 4 2240.0 5 1389.5 6 1780.0 7 3233.5 8 4980.0 9 5696.5 10 5020.5 11 4197.0 12 4289.5 13 4513.0 14 4773.0 15 5129.5 16 5429.5 17 5831.5 18 6184.5 19 6507.5 20 6968.5 21 7720.0 22 8797.0 23 10233.5 24 12229.0 25 14680.5 26 17979.5 27 21787.0 28 25705.0 29 30829.0 30 37545.0 31 43631.0 32 50598.0 33 59636.0 34 68291.0 35 75574.0 36 84566.5 37 92829.0 38 96671.0 39 99259.0 40 97263.5 41 94204.5 42 92596.0 43 91198.0 44 92072.0 45 93905.0 46 94810.0 47 94016.5 48 95035.0 49 99401.5 50 107009.5 51 105584.5 52 94071.0 53 85902.0 54 80220.5 55 73296.5 56 66071.0 57 60291.0 58 53806.5 59 45148.0 60 34334.0 61 24683.0 62 18643.0 63 14017.0 64 10213.5 65 7429.5 66 5382.5 67 4041.5 68 3043.0 69 2269.0 70 1789.0 71 1352.0 72 1045.5 73 859.0 74 665.0 75 463.5 76 312.0 77 214.5 78 147.0 79 96.0 80 63.0 81 41.0 82 29.5 83 26.5 84 17.5 85 11.5 86 7.0 87 4.5 88 4.0 89 5.0 90 3.0 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0037615223489954764 2 0.06785069837140412 3 5.731843579421678E-4 4 6.448324026849388E-4 5 0.0019344972080548167 6 7.164804474277098E-4 7 0.0 8 5.731843579421678E-4 9 3.582402237138549E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.627444138706315E-4 18-19 0.0010747206711415646 20-21 0.0034211941364673147 22-23 0.0 24-25 1.7912011185692743E-5 26-27 1.970321230426202E-4 28-29 0.0011284567046986429 30-31 0.0015046089395981906 32-33 0.0031346019574962306 34-35 0.004782506986579963 36-37 0.0010926326823272575 38-39 0.0014866969284124977 40-41 0.005337779333336438 42-43 0.00743348464206249 44-45 0.005803491624164449 46-47 0.007827548888147729 48-49 0.00881270950336083 50-51 0.008060405033561735 52-53 0.0037615223489954764 54-55 0.0022031773758402077 56-57 0.0029733938568249955 58-59 0.0034032821252816215 60-61 0.007809636876962037 62-63 0.014992353362424828 64-65 0.00437053072930903 66-67 0.010514350566001642 68-69 0.012860824031327392 70-71 0.006340851959735231 72-73 0.007970844977633272 74-75 0.0029733938568249955 76-77 0.0032958100581674653 78-79 0.008203701123047276 80-81 0.006036347769578455 82-83 0.017697067051464434 84-85 0.018574755599563376 86-87 0.024109567055942433 88-89 0.02303484638480087 90-91 0.02459319135795614 92-93 0.035268750024629016 94-95 0.03784807963536877 96-97 0.02459319135795614 98-99 0.0185210195660063 100 0.019344972080548164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2791423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.80607828003258 #Duplication Level Percentage of deduplicated Percentage of total 1 80.04668317701044 51.875116160263055 2 13.870388617588272 17.977709810517972 3 3.1876476482038707 6.197368290559854 4 1.0492318861554373 2.7198641499238203 5 0.5220440096323263 1.6915812476927317 6 0.30146432877128365 1.1722032533393572 7 0.18707538771760185 0.8486535554486048 8 0.1283912249963414 0.6656425422065743 9 0.09329261418878139 0.5441335612260342 >10 0.5301303631255042 6.261780730599686 >50 0.0445680958726581 2.024407571557485 >100 0.03516787645800978 4.253548671689517 >500 0.003030789893903768 1.2930971663384365 >1k 6.314145612272211E-4 0.831156914103272 >5k 1.262829122454442E-4 0.5850261126887791 >10k+ 1.262829122454442E-4 1.0587102618449171 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13961 0.5001391763269128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11912 0.426735754487944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8072 0.28917150858182367 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6225 0.2230045392618747 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4544 0.16278435765557567 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.12258980455488115 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.5824022371385494E-5 0.0 0.0 0.0 0.0 7 3.5824022371385494E-5 0.0 0.0 0.0 0.0 8 3.5824022371385494E-5 0.0 0.0 0.0 7.164804474277099E-5 9 3.5824022371385494E-5 0.0 0.0 0.0 7.164804474277099E-5 10-11 3.5824022371385494E-5 0.0 0.0 0.0 7.164804474277099E-5 12-13 3.5824022371385494E-5 0.0 0.0 0.0 7.164804474277099E-5 14-15 3.5824022371385494E-5 0.0 0.0 3.5824022371385494E-5 7.164804474277099E-5 16-17 3.5824022371385494E-5 0.0 0.0 3.5824022371385494E-5 8.956005592846373E-5 18-19 3.5824022371385494E-5 0.0 0.0 7.164804474277099E-5 1.0747206711415647E-4 20-21 3.5824022371385494E-5 0.0 0.0 7.164804474277099E-5 1.0747206711415647E-4 22-23 3.5824022371385494E-5 0.0 0.0 1.7912011185692745E-4 1.0747206711415647E-4 24-25 3.5824022371385494E-5 0.0 0.0 3.224162013424694E-4 1.0747206711415647E-4 26-27 7.164804474277099E-5 0.0 0.0 4.2988826845662587E-4 1.0747206711415647E-4 28-29 7.164804474277099E-5 0.0 0.0 0.0013792248612983413 1.0747206711415647E-4 30-31 7.164804474277099E-5 0.0 0.0 0.005033275143179661 1.0747206711415647E-4 32-33 7.164804474277099E-5 0.0 0.0 0.013756424590612028 2.5076815659969844E-4 34-35 7.164804474277099E-5 0.0 0.0 0.02565000001791201 2.8659217897108395E-4 36-37 7.164804474277099E-5 0.0 0.0 0.0446725558971177 2.8659217897108395E-4 38-39 7.164804474277099E-5 0.0 0.0 0.08212657128640125 2.8659217897108395E-4 40-41 7.164804474277099E-5 0.0 0.0 0.1338385475794962 2.8659217897108395E-4 42-43 7.164804474277099E-5 0.0 0.0 0.18452953923500665 3.0450419015677665E-4 44-45 7.164804474277099E-5 0.0 0.0 0.24098819849231018 3.224162013424694E-4 46-47 7.164804474277099E-5 0.0 0.0 0.3018890365236655 3.224162013424694E-4 48-49 7.164804474277099E-5 0.0 0.0 0.3748088340606207 3.224162013424694E-4 50-51 7.164804474277099E-5 0.0 0.0 0.4564876050673796 3.224162013424694E-4 52-53 7.164804474277099E-5 0.0 0.0 0.5443639319443883 3.4032821252816215E-4 54-55 7.164804474277099E-5 0.0 0.0 0.6586783873314793 3.582402237138549E-4 56-57 7.164804474277099E-5 0.0 0.0 0.7797456709355766 3.582402237138549E-4 58-59 7.164804474277099E-5 0.0 0.0 0.9098226961660774 4.2988826845662587E-4 60-61 7.164804474277099E-5 1.7912011185692747E-5 0.0 1.0494826473809238 4.2988826845662587E-4 62-63 7.164804474277099E-5 3.5824022371385494E-5 0.0 1.205639560897793 4.2988826845662587E-4 64-65 7.164804474277099E-5 3.5824022371385494E-5 0.0 1.3611874660343486 4.2988826845662587E-4 66-67 7.164804474277099E-5 3.5824022371385494E-5 0.0 1.5393761533096202 4.2988826845662587E-4 68-69 7.164804474277099E-5 3.5824022371385494E-5 0.0 1.7532993029003485 4.2988826845662587E-4 70-71 1.0747206711415647E-4 3.5824022371385494E-5 0.0 1.9951114539072008 4.2988826845662587E-4 72-73 1.0747206711415647E-4 3.5824022371385494E-5 0.0 2.222576083954313 4.2988826845662587E-4 74-75 1.0747206711415647E-4 3.5824022371385494E-5 0.0 2.4629194500439384 4.2988826845662587E-4 76-77 1.0747206711415647E-4 3.5824022371385494E-5 0.0 2.725330413914337 4.2988826845662587E-4 78-79 1.0747206711415647E-4 3.5824022371385494E-5 0.0 3.03064064457447 4.2988826845662587E-4 80-81 1.0747206711415647E-4 3.5824022371385494E-5 0.0 3.330613095901266 4.2988826845662587E-4 82-83 1.0747206711415647E-4 3.5824022371385494E-5 0.0 3.6569878517157735 4.2988826845662587E-4 84-85 1.0747206711415647E-4 3.5824022371385494E-5 0.0 4.001005938548189 4.478002796423186E-4 86-87 1.0747206711415647E-4 3.5824022371385494E-5 0.0 4.363133068689339 4.657122908280114E-4 88 1.0747206711415647E-4 3.5824022371385494E-5 0.0 4.651140296544092 4.657122908280114E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5350 0.0 64.24394 2 ACCTGGG 5565 0.0 59.360035 3 GTACCTG 5925 0.0 58.56794 1 TAACGCA 525 0.0 54.59772 4 TATAACG 550 0.0 51.29289 2 TATCACG 285 0.0 44.543823 2 CCTGGGG 3795 0.0 43.089497 4 GTACCCG 550 0.0 40.181587 1 ATAACGC 820 0.0 36.10195 3 TACCCGG 600 0.0 33.696575 2 GTATAAC 1105 0.0 31.914711 1 CTGGGGG 2640 0.0 30.25861 5 ATCACGC 420 0.0 30.207756 3 GTATCAA 15815 0.0 27.531744 1 CCTGGGA 4035 0.0 27.483524 4 TGGGGGG 4640 0.0 27.241468 6 TCACGCA 505 0.0 26.984268 4 ACAACGC 385 0.0 26.85134 3 GGTATCA 10960 0.0 26.51367 1 ATGGGGG 4045 0.0 26.48624 5 >>END_MODULE