FastQCFastQC Report
Mon 6 Jun 2016
ERR1211510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11277488
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458180.4062784194494377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381240.33805400635318783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252500.22389737856515565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216050.051.561791
GTATCAA394950.039.519271
ATCAACG473550.032.0510523
TCAACGC475250.031.8964274
CAACGCA479250.031.6671815
AACGCAG498150.030.5637766
TATCAAC505850.030.1644422
GTACATG506050.029.7917081
TACATGG523200.028.3193322
ACATGGG512450.027.6649193
ACGCAGA559150.026.9365847
CGCAGAG559800.026.7555548
CATGGGG217300.025.6030564
TACCTGG80950.024.5711692
GCAGAGT617050.023.5522529
GTACCTG91900.023.2086681
CATGGGA263950.021.4162674
GAGTACT358500.021.37047212-13
ATGGGGG127200.020.6487465
CAGAGTA609000.020.24852810-11
Produced by FastQC (version 0.11.5)