FastQCFastQC Report
Mon 6 Jun 2016
ERR1211510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11277488
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504730.4475553421116475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430030.38131718694801536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302510.26824236035542665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233750.050.8635861
GTATCAA420750.038.306411
ATCAACG520250.030.2239043
TCAACGC521650.030.2147244
CAACGCA528200.029.8667325
AACGCAG546100.028.9730476
TATCAAC550750.028.6997552
GTACATG521850.028.0913491
TACATGG539600.026.6519952
ACATGGG523200.026.4158123
ACGCAGA615050.025.503467
CGCAGAG617600.025.26128
CATGGGG211500.023.9965134
TACCTGG82800.022.9464342
GCAGAGT678400.022.7269789
GTACCTG92250.021.7692261
GAGTACT395650.021.46228612-13
CATGGGA274900.021.3512084
AGAGTAC644200.020.50890710-11
AGTACTT435750.019.5303512-13
Produced by FastQC (version 0.11.5)