##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11277488 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16399671629001 33.0 31.0 34.0 30.0 34.0 2 32.37980514809681 34.0 31.0 34.0 31.0 34.0 3 32.389121052489706 34.0 31.0 34.0 30.0 34.0 4 36.01550132440841 37.0 35.0 37.0 35.0 37.0 5 35.9491396488296 37.0 35.0 37.0 35.0 37.0 6 35.969179483941815 37.0 35.0 37.0 35.0 37.0 7 35.8965846826882 37.0 35.0 37.0 35.0 37.0 8 35.89227255218538 37.0 35.0 37.0 35.0 37.0 9 37.544377613170596 39.0 37.0 39.0 35.0 39.0 10-11 37.51311320393336 39.0 37.0 39.0 35.0 39.0 12-13 37.44293680472105 39.0 37.0 39.0 34.0 39.0 14-15 38.67983734498321 40.0 38.0 41.0 34.0 41.0 16-17 38.62640035617861 40.0 38.0 41.0 34.0 41.0 18-19 38.643942959637826 40.0 38.0 41.0 34.0 41.0 20-21 38.60044435427464 40.0 38.0 41.0 34.0 41.0 22-23 38.50056892102213 40.0 38.0 41.0 34.0 41.0 24-25 38.404140798021686 40.0 38.0 41.0 34.0 41.0 26-27 38.24194040374949 40.0 38.0 41.0 34.0 41.0 28-29 38.274113747671464 40.0 38.0 41.0 34.0 41.0 30-31 38.19115302982367 40.0 38.0 41.0 34.0 41.0 32-33 38.01009617567316 40.0 37.0 41.0 33.0 41.0 34-35 37.903814794571275 40.0 37.0 41.0 33.0 41.0 36-37 37.84702834531946 40.0 37.0 41.0 33.0 41.0 38-39 37.760855209954556 40.0 37.0 41.0 33.0 41.0 40-41 37.612978971912895 40.0 37.0 41.0 32.5 41.0 42-43 37.468838184531876 40.0 37.0 41.0 32.0 41.0 44-45 37.285212584575575 40.0 36.5 41.0 31.0 41.0 46-47 37.0234001579075 40.0 36.0 41.0 30.5 41.0 48-49 36.80051931777715 39.5 36.0 41.0 30.0 41.0 50-51 36.61112714107965 39.0 36.0 41.0 30.0 41.0 52-53 36.43778814040857 39.0 35.0 41.0 29.0 41.0 54-55 36.26661158938941 39.0 35.0 40.0 28.5 41.0 56-57 36.09301206970914 39.0 35.0 40.0 28.0 41.0 58-59 35.862749576856125 38.5 35.0 40.0 28.0 41.0 60-61 35.58420332613078 38.0 34.0 40.0 27.0 41.0 62-63 35.29325706221101 38.0 34.0 40.0 26.5 41.0 64-65 35.00798577661976 37.5 34.0 40.0 26.0 41.0 66-67 34.69171654183981 37.0 33.5 40.0 26.0 41.0 68-69 34.3159230583974 36.5 33.0 39.0 26.0 41.0 70-71 33.94285757608432 36.0 33.0 39.0 25.0 40.0 72-73 33.5413584567769 35.5 32.0 38.5 24.5 40.0 74-75 33.13171297544275 35.0 32.0 38.0 24.0 40.0 76-77 31.556768271444845 34.0 30.0 36.0 21.5 38.5 78-79 32.36526547401336 35.0 31.0 37.0 23.5 39.0 80-81 32.30416667257815 35.0 31.0 36.0 23.5 39.0 82-83 31.995238301295466 35.0 31.0 36.0 22.5 37.5 84-85 31.66989164608289 34.0 31.0 35.5 22.0 37.0 86-87 31.361198566560212 34.0 31.0 35.0 20.5 37.0 88-89 31.059532362171435 34.0 30.5 35.0 20.0 36.0 90-91 30.76457115272479 34.0 30.0 35.0 20.0 36.0 92-93 30.543027977507048 34.0 30.0 35.0 19.0 35.5 94-95 30.31537049740155 34.0 29.0 35.0 19.0 35.0 96-97 30.114286443931483 34.0 29.0 35.0 18.0 35.0 98-99 29.95494736948512 34.0 29.0 35.0 18.0 35.0 100 28.110980565884883 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3057905765045419 1101 2 0.2532636421483687 1101 3 0.27604806091751044 1101 4 0.17185242153944813 1101 5 0.1897172043242108 1101 6 0.18931036954174374 1101 7 0.2329461088117526 1101 8 0.1966455802978473 1101 9 0.22698852073733633 1101 10-11 0.22646146559305436 1101 12-13 0.25340499552899587 1101 14-15 0.32086536584809977 1101 16-17 0.3190992311808927 1101 18-19 0.34403569842762494 1101 20-21 0.3182033337435968 1101 22-23 0.34571025679891676 1101 24-25 0.30827933416350817 1101 26-27 0.28335997377516975 1101 28-29 0.3073651993412767 1101 30-31 0.34910678222368574 1101 32-33 0.4225249682117962 1101 34-35 0.3904948040938905 1101 36-37 0.421114518249901 1101 38-39 0.44187279499080745 1101 40-41 0.44795466957462793 1101 42-43 0.42530575731578324 1101 44-45 0.39382990211586844 1101 46-47 0.4376242433584352 1101 48-49 0.5095567525995861 1101 50-51 0.49454628737449013 1101 52-53 0.474827209273343 1101 54-55 0.40984703935082933 1101 56-57 0.42956889653337527 1101 58-59 0.4936102091200496 1101 60-61 0.40506807488429786 1101 62-63 0.4591235416923709 1101 64-65 0.43045547453970556 1101 66-67 0.3608037727270741 1101 68-69 0.42379727289342384 1101 70-71 0.3608818494857786 1101 72-73 0.28478835479197784 1101 74-75 0.3846011706396055 1101 76-77 0.3543249250471554 1101 78-79 0.44644687994423293 1101 80-81 0.43442245345386965 1101 82-83 0.4064982389573295 1101 84-85 0.39391029815639556 1101 86-87 0.33859402042420683 1101 88-89 0.26341488156365145 1101 90-91 0.28908065491337354 1101 92-93 0.24262388887911612 1101 94-95 0.2173791504083411 1101 96-97 0.2148228839775861 1101 98-99 0.17774261658220425 1101 100 0.22914979471306296 1102 1 0.35779902565976585 1102 2 0.2647504393734934 1102 3 0.32916050586756285 1102 4 0.2169138069021983 1102 5 0.18187791672222176 1102 6 0.17788265433413386 1102 7 0.23363571582711273 1102 8 0.21726863760798665 1102 9 0.2568932349819022 1102 10-11 0.26856216493715834 1102 12-13 0.30419914047390506 1102 14-15 0.4168682423626109 1102 16-17 0.41244449341795786 1102 18-19 0.37632711475702507 1102 20-21 0.4058133827296828 1102 22-23 0.3846473138898716 1102 24-25 0.3609252425980216 1102 26-27 0.35636600902459037 1102 28-29 0.46925584329841286 1102 30-31 0.47939429789855836 1102 32-33 0.5530952958881201 1102 34-35 0.539080860466207 1102 36-37 0.5772216029519015 1102 38-39 0.5689609010979169 1102 40-41 0.5535438361903786 1102 42-43 0.5057087893909582 1102 44-45 0.5251382700963632 1102 46-47 0.5756762533787168 1102 48-49 0.5933603853872 1102 50-51 0.5939243744632492 1102 52-53 0.6283603371967672 1102 54-55 0.6310124835473232 1102 56-57 0.5875806877213137 1102 58-59 0.6512555792991463 1102 60-61 0.5885258082568114 1102 62-63 0.6238059661499236 1102 64-65 0.6252437171018386 1102 66-67 0.6138815850631119 1102 68-69 0.6346703619654761 1102 70-71 0.5696220020787663 1102 72-73 0.5628659586146583 1102 74-75 0.5921892626841867 1102 76-77 0.6124168047611356 1102 78-79 0.6215474367233966 1102 80-81 0.564175146913044 1102 82-83 0.5302341679425275 1102 84-85 0.5097376355183947 1102 86-87 0.49694238126675927 1102 88-89 0.4761560078655229 1102 90-91 0.5251747051059361 1102 92-93 0.48732885217591715 1102 94-95 0.5175303759334824 1102 96-97 0.5036900842444787 1102 98-99 0.5024185140867345 1102 100 0.5646046496314092 1103 1 0.3226778102409824 1103 2 0.2585092039498278 1103 3 0.3319307202877084 1103 4 0.16663702236014188 1103 5 0.1826250054688643 1103 6 0.20314351553932397 1103 7 0.21559110993973007 1103 8 0.2371451157851041 1103 9 0.26835835210453496 1103 10-11 0.2491867301791828 1103 12-13 0.30354772977957367 1103 14-15 0.3901059201138253 1103 16-17 0.3852308703219478 1103 18-19 0.38054127938452353 1103 20-21 0.3347341782127913 1103 22-23 0.33986781222081675 1103 24-25 0.35822606927617073 1103 26-27 0.3706932307883122 1103 28-29 0.41479921894310934 1103 30-31 0.4198445394370083 1103 32-33 0.4802219015835405 1103 34-35 0.4739188231393783 1103 36-37 0.5141361318211182 1103 38-39 0.4818679075908392 1103 40-41 0.5224402065812228 1103 42-43 0.5394321762643202 1103 44-45 0.4860804193182986 1103 46-47 0.6172314695274608 1103 48-49 0.5823690039875729 1103 50-51 0.5688857048780207 1103 52-53 0.5330645380622556 1103 54-55 0.5521110637846647 1103 56-57 0.5583495500782547 1103 58-59 0.6432896543733264 1103 60-61 0.5915670452213035 1103 62-63 0.6589197631972823 1103 64-65 0.682744566956373 1103 66-67 0.5916925941060782 1103 68-69 0.6374109414202138 1103 70-71 0.615454007263601 1103 72-73 0.5846454368505221 1103 74-75 0.5497284916165484 1103 76-77 0.6439455060903079 1103 78-79 0.6585348203559178 1103 80-81 0.5612058498542964 1103 82-83 0.5326926213709733 1103 84-85 0.5059537171737709 1103 86-87 0.5353008121929257 1103 88-89 0.5045395155193511 1103 90-91 0.5872039105697873 1103 92-93 0.5400666068072617 1103 94-95 0.5871727918123284 1103 96-97 0.6166260515972262 1103 98-99 0.633684834263132 1103 100 0.6753181711300797 1104 1 0.29872525344041634 1104 2 0.2569917590092814 1104 3 0.2932492343974715 1104 4 0.11919621530325486 1104 5 0.16964027090544676 1104 6 0.20991901354081932 1104 7 0.2287788884211963 1104 8 0.1994348759847 1104 9 0.286699624719958 1104 10-11 0.31102518988239325 1104 12-13 0.3034864195855391 1104 14-15 0.4301047028893663 1104 16-17 0.3600943513958512 1104 18-19 0.37828083237183563 1104 20-21 0.30991757997123415 1104 22-23 0.38520331739353253 1104 24-25 0.41018682431246134 1104 26-27 0.43788329627663103 1104 28-29 0.5214292939619298 1104 30-31 0.43538270023201164 1104 32-33 0.48150700724969653 1104 34-35 0.5316051415094947 1104 36-37 0.4593456890894245 1104 38-39 0.49791614431440223 1104 40-41 0.5705237829393326 1104 42-43 0.6394253987267078 1104 44-45 0.6054889844607985 1104 46-47 0.5718564677363887 1104 48-49 0.6043036094147496 1104 50-51 0.602999036133518 1104 52-53 0.5970461928002209 1104 54-55 0.6439598725254783 1104 56-57 0.6928600429854086 1104 58-59 0.6838721892149735 1104 60-61 0.6285308162522014 1104 62-63 0.6591168582498597 1104 64-65 0.8194422348125485 1104 66-67 0.7707133995623252 1104 68-69 0.7642037482791437 1104 70-71 0.742513955061618 1104 72-73 0.755284085624389 1104 74-75 0.7917155333905086 1104 76-77 0.6686776304422857 1104 78-79 0.7620821900844561 1104 80-81 0.8383880505255661 1104 82-83 0.6789947180872566 1104 84-85 0.6675747229146829 1104 86-87 0.7440014118039251 1104 88-89 0.7794962247755315 1104 90-91 0.7060176725462313 1104 92-93 0.6861544289171597 1104 94-95 0.7226839512361352 1104 96-97 0.6954097022446497 1104 98-99 0.7560410349839977 1104 100 0.8303050245543062 1105 1 0.2385392574627474 1105 2 0.15225565371354577 1105 3 0.21635735845101323 1105 4 0.11029065441444885 1105 5 0.08883969458724295 1105 6 0.15985486087925693 1105 7 0.16497999314002953 1105 8 0.11552905197420671 1105 9 0.13862938528576052 1105 10-11 0.16713658481626936 1105 12-13 0.2121851374148207 1105 14-15 0.26385578520199715 1105 16-17 0.2500127706000086 1105 18-19 0.22111146601132958 1105 20-21 0.22555107075265113 1105 22-23 0.2693040671759377 1105 24-25 0.2604559928904351 1105 26-27 0.3184435656870832 1105 28-29 0.3085262446594612 1105 30-31 0.35774591209465 1105 32-33 0.38146474108233264 1105 34-35 0.44611019981954314 1105 36-37 0.46813597326411127 1105 38-39 0.4258479196405247 1105 40-41 0.46944188868548764 1105 42-43 0.4082351473686927 1105 44-45 0.42285819255605617 1105 46-47 0.45409362960459987 1105 48-49 0.47001666427055255 1105 50-51 0.4561377084509317 1105 52-53 0.46793231144815195 1105 54-55 0.4923409747013494 1105 56-57 0.455303145781663 1105 58-59 0.4534522273986781 1105 60-61 0.5134991844499766 1105 62-63 0.4969033250355679 1105 64-65 0.5323222143903763 1105 66-67 0.5181133389626211 1105 68-69 0.5704043365604008 1105 70-71 0.5623584877362617 1105 72-73 0.5356828473508699 1105 74-75 0.5929640931670122 1105 76-77 0.5735884158490698 1105 78-79 0.6676559440725498 1105 80-81 0.6737154670530714 1105 82-83 0.6049901368282526 1105 84-85 0.5865739420479805 1105 86-87 0.6181665038262416 1105 88-89 0.6056060279049476 1105 90-91 0.6586276206377129 1105 92-93 0.6100120902197439 1105 94-95 0.6420132360910173 1105 96-97 0.7292961336968737 1105 98-99 0.7259305663691826 1105 100 0.8394202460292206 1106 1 0.20670769199933403 1106 2 0.16313549867988542 1106 3 0.1955614944372286 1106 4 0.1239111563517099 1106 5 0.10691703895128057 1106 6 0.11702867604216749 1106 7 0.1554528615918116 1106 8 0.17369021272552487 1106 9 0.13632994757240624 1106 10-11 0.17265145032694562 1106 12-13 0.22737883403807757 1106 14-15 0.25566574653574037 1106 16-17 0.2914756341411646 1106 18-19 0.2756033015987711 1106 20-21 0.2673865792001209 1106 22-23 0.2771156682380962 1106 24-25 0.2644586976853418 1106 26-27 0.2941470361713172 1106 28-29 0.28913753554898847 1106 30-31 0.305158998588837 1106 32-33 0.3763746317188392 1106 34-35 0.3632029402527053 1106 36-37 0.37377510201603314 1106 38-39 0.31789446989151315 1106 40-41 0.360120388034467 1106 42-43 0.33462596423123614 1106 44-45 0.3871395590271334 1106 46-47 0.45130623938609205 1106 48-49 0.46985716760332963 1106 50-51 0.48580150158841917 1106 52-53 0.548855167195498 1106 54-55 0.45807072296913276 1106 56-57 0.4917409032440361 1106 58-59 0.5941043304771014 1106 60-61 0.6333360706379452 1106 62-63 0.6371834645079346 1106 64-65 0.6320408604262653 1106 66-67 0.6204641012948144 1106 68-69 0.6010088769822346 1106 70-71 0.6255374544506367 1106 72-73 0.6316436485599652 1106 74-75 0.657673987621429 1106 76-77 0.5689519109259358 1106 78-79 0.6913905259516753 1106 80-81 0.70931594929516 1106 82-83 0.6961521125068124 1106 84-85 0.6268630667845123 1106 86-87 0.588578927451497 1106 88-89 0.5856171426438088 1106 90-91 0.65050320073032 1106 92-93 0.6900561673837409 1106 94-95 0.8014591810050931 1106 96-97 0.8104899988349707 1106 98-99 0.8396461771630861 1106 100 0.8581856575674855 1107 1 0.1884447457952163 1107 2 0.17035107721899578 1107 3 0.21765802535728795 1107 4 0.15565898368777198 1107 5 0.12104025233919913 1107 6 0.13399789962904407 1107 7 0.1662910430182336 1107 8 0.14118823757648613 1107 9 0.17038780148320143 1107 10-11 0.15575180585376813 1107 12-13 0.16330320378597918 1107 14-15 0.22759753207046174 1107 16-17 0.17382363744504659 1107 18-19 0.16473998191192862 1107 20-21 0.19731143375732785 1107 22-23 0.2741484557120657 1107 24-25 0.2740615131705155 1107 26-27 0.2697077091584603 1107 28-29 0.2954203050670543 1107 30-31 0.26779456563618 1107 32-33 0.27025885207364553 1107 34-35 0.3217068546389683 1107 36-37 0.3398824423880811 1107 38-39 0.3615300010270488 1107 40-41 0.3319489271424203 1107 42-43 0.33017065646709653 1107 44-45 0.34488618331786824 1107 46-47 0.30400860238257366 1107 48-49 0.3699083419192064 1107 50-51 0.35721705498927037 1107 52-53 0.3577635382362203 1107 54-55 0.4461978327874192 1107 56-57 0.45360616216812133 1107 58-59 0.5225428853862368 1107 60-61 0.4782936049337181 1107 62-63 0.4739059740245608 1107 64-65 0.5687050132668858 1107 66-67 0.5511637529503446 1107 68-69 0.4510070813922056 1107 70-71 0.5858481813054794 1107 72-73 0.49468726344181135 1107 74-75 0.5215449206100118 1107 76-77 0.43119603934653483 1107 78-79 0.5618234427081177 1107 80-81 0.5329683589769871 1107 82-83 0.5612844474920955 1107 84-85 0.47134701420963765 1107 86-87 0.4242406921573796 1107 88-89 0.48561669374630156 1107 90-91 0.33843952910782704 1107 92-93 0.4205497750832343 1107 94-95 0.6432928375446316 1107 96-97 0.6355999787243611 1107 98-99 0.7070535247174909 1107 100 0.7594766867996903 1108 1 0.2308245897348371 1108 2 0.18820278466851192 1108 3 0.23536851711735807 1108 4 0.12858289803716616 1108 5 0.09917256783456452 1108 6 0.13462714139242138 1108 7 0.1330479765795758 1108 8 0.1529292718853199 1108 9 0.14509491041795286 1108 10-11 0.18831210485392802 1108 12-13 0.16064551061720067 1108 14-15 0.22667585242875532 1108 16-17 0.24407144335878428 1108 18-19 0.26953999217975166 1108 20-21 0.24854965619019964 1108 22-23 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-0.3116610933346706 2316 56-57 -0.6169536839316834 2316 58-59 -0.5199588711077539 2316 60-61 -0.31412229872091046 2316 62-63 -0.3685479823383133 2316 64-65 -0.49502814061317935 2316 66-67 -0.21294401243127936 2316 68-69 -0.28629503333705486 2316 70-71 -0.4463892871727211 2316 72-73 -0.4578650929452266 2316 74-75 -0.5532819752287779 2316 76-77 -0.5196567272993242 2316 78-79 -0.42646393089703594 2316 80-81 -0.2666744685979552 2316 82-83 -0.22003789600652723 2316 84-85 -0.32765958774644943 2316 86-87 -0.3612884125005742 2316 88-89 -0.29678098488466276 2316 90-91 -0.2469170167970809 2316 92-93 -0.14721042097282222 2316 94-95 -0.07435363391625671 2316 96-97 -0.0987703153821009 2316 98-99 -0.2221706195790496 2316 100 -0.46153568026056746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 12.0 11 38.0 12 124.0 13 356.0 14 941.0 15 2666.0 16 6162.0 17 13212.0 18 24287.0 19 38332.0 20 53562.0 21 75331.0 22 97571.0 23 105140.0 24 106827.0 25 113106.0 26 130358.0 27 156291.0 28 189777.0 29 230287.0 30 278436.0 31 338652.0 32 415148.0 33 520656.0 34 683708.0 35 929785.0 36 1364880.0 37 2233328.0 38 2652730.0 39 515684.0 40 94.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90819367728079 21.631224666603412 14.513026830455674 22.94755482566012 2 14.029819092418421 22.365011460349322 41.42966415807917 22.17550528915309 3 16.190380761523045 29.170198804689022 31.62596875166262 23.013451682125314 4 10.85804585021959 18.815347416464906 40.77470406341311 29.551902669902397 5 11.126563982513545 40.02117528131734 36.58709974905341 12.26516098711571 6 27.83581961836774 40.75707691869784 18.960530001549117 12.446573461385297 7 24.90360441970765 34.18953759915328 24.476434823074076 16.43042315806499 8 24.583602123721167 37.2160276660127 21.38984471120274 16.810525499063388 9 26.276029298733654 15.302438026070353 21.628982138214518 36.79255053698148 10-11 23.072154898324875 27.786515933335508 30.235815812883153 18.905513355456463 12-13 24.109593377532303 25.793026780431955 30.06836717538516 20.029012666650587 14-15 21.55163454840298 26.891697867468356 27.865017457788472 23.691650126340193 16-17 20.381579855682205 29.937099439600612 28.526801404241674 21.154519300475506 18-19 20.304793046810524 28.808860090178932 30.861557366677317 20.024789496333227 20-21 21.32595934038245 27.98898263877081 30.821609844117425 19.863448176729314 22-23 20.97409644542264 28.08652317666161 30.45808875683031 20.481291621085443 24-25 20.68655989967268 28.28219302235476 30.675226257198357 20.356020820774205 26-27 20.55732370764979 28.366803096528542 30.6871201357929 20.38875306002877 28-29 20.536690045462226 28.07631946928829 30.977745770750303 20.40924471449918 30-31 20.857922716406783 28.119118305656148 30.761424079032324 20.261534898904742 32-33 20.621490656952254 28.37552573777979 30.685750366327742 20.31723323894021 34-35 20.84374538884128 28.446792371582774 30.66702095736168 20.042441282214266 36-37 20.926856571090195 28.21468164161669 30.445291000824486 20.413170786468633 38-39 20.69365695443064 28.50998467851823 30.595860083042016 20.200498284009115 40-41 20.770930476678835 28.30970855943305 30.70434925070903 20.215011713179084 42-43 20.683510049151018 28.257476450946438 30.436514348113636 20.622499151788904 44-45 20.723095833848216 28.542822547316092 30.218322136274722 20.51575948256097 46-47 20.748913042803533 28.565400952391677 29.979289972574335 20.706396032230458 48-49 20.706732961503814 28.613937984625366 29.993728547188514 20.68560050668231 50-51 20.864053179597757 28.684233279726296 29.67997619829903 20.771737342376912 52-53 20.87338105962186 28.80008966913093 29.56438776986478 20.762141501382438 54-55 20.85469517802425 28.881376591832726 29.512170714866752 20.75175751527627 56-57 20.914534160301013 28.8557054668967 29.3753919418181 20.854368430984188 58-59 20.800891894779827 28.712099681523156 29.702964809705318 20.7840436139917 60-61 21.01230748452536 29.098836452692918 29.336545650953898 20.552310411827825 62-63 20.754078282990648 28.911615363228695 29.68522648111434 20.649079872666317 64-65 20.716136889525462 28.674414999867874 29.612886611925187 20.996561498681476 66-67 20.754887734798054 28.79482543606343 29.368740889364318 21.081545939774195 68-69 20.702536310719267 28.93380998397177 29.426567040114737 20.937086665194222 70-71 20.80218412298661 28.726378295333244 29.39632232884441 21.075115252835737 72-73 20.76980517210273 28.927729814859426 29.17154014641035 21.130924866627495 74-75 20.727500605983 29.032835167347297 29.059495315807926 21.180168910861774 76-77 20.560576901973974 28.888837654430326 29.357879762430745 21.19270568116496 78-79 20.574593517546084 28.765076734817157 29.203907254364648 21.456422493272107 80-81 20.559952174309974 29.17583465392994 28.90934468065786 21.354868491102227 82-83 20.742563685235 29.03089872194964 28.97706572379397 21.249471869021388 84-85 20.59495131654542 29.007035079793397 28.895647498903376 21.502366104757805 86-87 20.390424479592344 29.05208347099089 29.159010689085434 21.39848136033133 88-89 20.562603748974013 28.862823219087456 29.08434652730485 21.490226504633682 90-91 20.706163251802927 29.00569237578195 28.93036019487828 21.357784177536846 92-93 20.6578002799286 28.93938656092572 28.811157653520986 21.591655505624697 94-95 20.36672487273982 28.780870323462842 28.972112090970015 21.88029271282732 96-97 20.51102016670218 28.738545073247895 28.974835469679437 21.77559929037049 98-99 20.320260999393504 28.705059856483505 28.940607445650514 22.03407169847248 100 19.525817575248176 29.176299428406622 29.02902205107561 22.268860945269594 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3991.0 1 2784.5 2 2259.0 3 3113.5 4 2700.5 5 2608.5 6 4122.5 7 8280.5 8 13879.0 9 16796.0 10 14898.5 11 12398.5 12 12670.5 13 13714.0 14 14700.0 15 15602.5 16 16852.5 17 18273.5 18 19866.0 19 21837.0 20 24958.5 21 29546.0 22 35455.0 23 44141.5 24 54738.0 25 67903.0 26 85725.5 27 104609.0 28 124026.5 29 149172.0 30 181463.0 31 212331.0 32 248544.5 33 294127.5 34 341413.5 35 380346.0 36 419573.5 37 454831.0 38 469279.5 39 476286.0 40 462738.5 41 441998.5 42 426550.5 43 415317.5 44 412559.0 45 414333.0 46 405773.0 47 387346.5 48 376933.0 49 366836.0 50 357751.5 51 332696.5 52 297108.5 53 271289.5 54 247838.5 55 222777.5 56 195297.5 57 166570.0 58 140742.0 59 118015.0 60 96020.5 61 76371.5 62 60201.5 63 45918.0 64 33785.5 65 24454.0 66 17292.0 67 12406.0 68 9110.5 69 6569.0 70 4735.5 71 3442.0 72 2544.0 73 1867.5 74 1400.0 75 1035.0 76 724.0 77 444.5 78 261.5 79 166.5 80 115.0 81 86.5 82 63.5 83 40.5 84 26.0 85 23.5 86 18.5 87 10.5 88 9.5 89 6.0 90 4.5 91 6.0 92 5.5 93 4.5 94 3.0 95 1.5 96 1.5 97 2.5 98 4.5 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003351810261292231 2 0.07269349344463945 3 7.803156163854928E-4 4 6.384400497699488E-4 5 0.001667037907732644 6 7.714483934720214E-4 7 0.0 8 0.0010019961892222809 9 3.546889165388604E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.492803361883426E-4 18-19 0.001161606201664768 20-21 0.003347376649835495 22-23 8.86722291347151E-6 24-25 1.3300834370207265E-5 26-27 1.8177806972616596E-4 28-29 9.177575715443013E-4 30-31 0.0015827992900546645 32-33 0.003187766637393008 34-35 0.004398142565081869 36-37 0.0014719590036362707 38-39 0.0016360026275354937 40-41 0.005856800734347933 42-43 0.00721791945156581 44-45 0.0056971907219054456 46-47 0.007315458903613996 48-49 0.008814019575990682 50-51 0.008716480123942496 52-53 0.004207497272442231 54-55 0.0024340526897479297 56-57 0.002695635765695339 58-59 0.0033074741467248735 60-61 0.007931730896100267 62-63 0.014214158330294832 64-65 0.004274001444293268 66-67 0.01042342053478576 68-69 0.012808703498509596 70-71 0.005768128505213218 72-73 0.007931730896100267 74-75 0.0031611649686525936 76-77 0.003152297745739122 78-79 0.008202181194961147 80-81 0.006238091319627208 82-83 0.016634910185672553 84-85 0.017681242489462193 86-87 0.02242520674816945 88-89 0.021072955253865044 90-91 0.024455800795354428 92-93 0.03457330213962542 94-95 0.03833300465493734 96-97 0.02297054095734795 98-99 0.018262045590294575 100 0.01919753760766582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1277488E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.86651921302624 #Duplication Level Percentage of deduplicated Percentage of total 1 67.44578071415835 32.28394758391815 2 17.51670424425868 16.769273205134127 3 6.693301199547089 9.61155091400077 4 3.175782713850501 6.080546571556864 5 1.7335103128545386 4.1488552348115455 6 1.0013485160849191 2.8758640790468455 7 0.6329831071619136 2.1209088642340745 8 0.3979154104879096 1.5237460513022998 9 0.28239725086081563 1.2165636090631518 >10 1.0117883837104527 7.783864508163623 >50 0.04916919897491014 1.6452759891021134 >100 0.04653613447010288 4.901008939749517 >500 0.007964192773885666 2.714010694647282 >1k 0.004584382294801412 3.9500517909273967 >5k 1.3385057790206344E-4 0.392746725165898 >10k+ 1.0038793342654758E-4 1.9817852391762263 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50473 0.4475553421116475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43003 0.38131718694801536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30251 0.26824236035542665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.86722291347151E-6 0.0 2 0.0 0.0 0.0 1.773444582694302E-5 0.0 3 0.0 0.0 0.0 1.773444582694302E-5 0.0 4 0.0 0.0 0.0 2.660166874041453E-5 0.0 5 0.0 0.0 0.0 2.660166874041453E-5 0.0 6 0.0 0.0 0.0 4.433611456735755E-5 0.0 7 0.0 0.0 0.0 4.433611456735755E-5 8.86722291347151E-6 8 0.0 0.0 0.0 4.433611456735755E-5 8.86722291347151E-6 9 0.0 0.0 0.0 7.98050062212436E-5 1.773444582694302E-5 10-11 0.0 0.0 0.0 1.0197306350492237E-4 1.773444582694302E-5 12-13 0.0 8.86722291347151E-6 0.0 1.2414112078860116E-4 2.2168057283678776E-5 14-15 0.0 2.2168057283678776E-5 0.0 1.285747322453369E-4 6.650417185103633E-5 16-17 0.0 2.660166874041453E-5 0.0 1.5074278952901567E-4 7.093778330777208E-5 18-19 0.0 3.546889165388604E-5 0.0 1.8177806972616598E-4 8.423861767797935E-5 20-21 0.0 4.433611456735755E-5 0.0 2.1281334992331624E-4 8.86722291347151E-5 22-23 0.0 4.433611456735755E-5 0.0 2.8818474468782413E-4 1.0640667496165812E-4 24-25 0.0 4.433611456735755E-5 0.0 3.6798975090906766E-4 1.197075093318654E-4 26-27 0.0 4.433611456735755E-5 0.0 5.009980946111403E-4 1.285747322453369E-4 28-29 0.0 4.433611456735755E-5 0.0 0.001356685105761141 1.3300834370207266E-4 30-31 0.0 5.763694893756482E-5 0.0 0.00499224650028446 1.4630917807227991E-4 32-33 0.0 7.093778330777208E-5 0.0 0.012737765715201826 1.7291084681269445E-4 34-35 0.0 8.423861767797935E-5 0.0 0.024921329998311682 1.773444582694302E-4 36-37 0.0 1.0640667496165812E-4 0.0 0.045732702176229316 1.773444582694302E-4 38-39 0.0 1.1527389787512964E-4 0.0 0.0803148715387682 1.9507890409637324E-4 40-41 0.0 1.1527389787512964E-4 0.0 0.11930848430075917 2.0394612700984474E-4 42-43 0.0 1.1527389787512964E-4 0.0 0.1574020739370328 2.0394612700984474E-4 44-45 4.433611456735755E-6 1.1527389787512964E-4 0.0 0.2044870276075665 2.083797384665805E-4 46-47 8.86722291347151E-6 1.1527389787512964E-4 0.0 0.2639594916882199 2.17246961380052E-4 48-49 8.86722291347151E-6 1.1527389787512964E-4 0.0 0.32996266544464514 2.3941501866373078E-4 50-51 8.86722291347151E-6 1.1527389787512964E-4 0.0 0.4028867066850348 2.482822415772023E-4 52-53 8.86722291347151E-6 1.1527389787512964E-4 0.0 0.47544719178597217 2.7931752177435263E-4 54-55 8.86722291347151E-6 1.2414112078860116E-4 0.0 0.5553586046821775 3.2365363634171015E-4 56-57 8.86722291347151E-6 1.3300834370207266E-4 0.0 0.652295085572248 3.4138808216865315E-4 58-59 8.86722291347151E-6 1.684772353559587E-4 0.0 0.7650418249170383 3.458216936253889E-4 60-61 8.86722291347151E-6 1.684772353559587E-4 0.0 0.8904509585822658 3.458216936253889E-4 62-63 8.86722291347151E-6 1.7291084681269445E-4 0.0 1.018750806917285 3.546889165388604E-4 64-65 1.3300834370207265E-5 2.1281334992331624E-4 0.0 1.1495955482284708 3.546889165388604E-4 66-67 1.773444582694302E-5 2.1281334992331624E-4 0.0 1.3010122466989102 3.635561394523319E-4 68-69 2.2168057283678776E-5 2.1281334992331624E-4 0.0 1.4797665934115822 3.6798975090906766E-4 70-71 2.660166874041453E-5 2.2611418429352353E-4 0.0 1.6830388114800034 3.8129058527927497E-4 72-73 2.660166874041453E-5 2.3054779575025928E-4 0.0 1.8899820598346015 4.4336114567357555E-4 74-75 2.660166874041453E-5 2.3054779575025928E-4 0.0 2.0949301830336684 4.477947571303113E-4 76-77 2.660166874041453E-5 2.3054779575025928E-4 0.0 2.3163669072403357 4.6109559150051856E-4 78-79 2.660166874041453E-5 2.3941501866373078E-4 0.0 2.5762696444456425 4.6109559150051856E-4 80-81 2.660166874041453E-5 2.3941501866373078E-4 0.0 2.8680278799675953 4.743964258707258E-4 82-83 2.660166874041453E-5 2.3941501866373078E-4 0.0 3.1766338390251447 5.054317060678761E-4 84-85 2.660166874041453E-5 2.3941501866373078E-4 0.0 3.478673619515268 5.098653175246118E-4 86-87 2.660166874041453E-5 2.3941501866373078E-4 0.0 3.787363817190495 5.142989289813476E-4 88 2.660166874041453E-5 2.482822415772023E-4 0.0 4.038913630411312 5.231661518948191E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23375 0.0 50.863586 1 GTATCAA 42075 0.0 38.30641 1 ATCAACG 52025 0.0 30.223904 3 TCAACGC 52165 0.0 30.214724 4 CAACGCA 52820 0.0 29.866732 5 AACGCAG 54610 0.0 28.973047 6 TATCAAC 55075 0.0 28.699755 2 GTACATG 52185 0.0 28.091349 1 TACATGG 53960 0.0 26.651995 2 ACATGGG 52320 0.0 26.415812 3 ACGCAGA 61505 0.0 25.50346 7 CGCAGAG 61760 0.0 25.2612 8 CATGGGG 21150 0.0 23.996513 4 TACCTGG 8280 0.0 22.946434 2 GCAGAGT 67840 0.0 22.726978 9 GTACCTG 9225 0.0 21.769226 1 GAGTACT 39565 0.0 21.462286 12-13 CATGGGA 27490 0.0 21.351208 4 AGAGTAC 64420 0.0 20.508907 10-11 AGTACTT 43575 0.0 19.53035 12-13 >>END_MODULE