FastQCFastQC Report
Mon 6 Jun 2016
ERR1211509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421665
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220580.2972109358210051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200770.27051881215333756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122710.1653402572064355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.050.0292431
GTATCAA198350.037.797371
TCAACGC237450.030.5916864
ATCAACG238750.030.4261383
CAACGCA239300.030.3539545
AACGCAG248700.029.258236
TATCAAC254350.028.768022
ACGCAGA280750.025.6513047
GTACATG225350.025.4112931
CGCAGAG286250.024.9790958
TACCTGG59700.024.8911462
TACATGG231950.024.044872
ACATGGG228600.023.4936643
GTACCTG64600.023.4732971
GCAGAGT317150.021.9550769
CATGGGG129900.021.593914
GAGTACT198750.020.9814112-13
CAGAGTA309550.019.82241210-11
AGAGTAC292250.019.42032610-11
ACCTGGG74550.019.2865493
Produced by FastQC (version 0.11.5)