##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211509_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7421665 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.056602797350727 31.0 31.0 34.0 28.0 34.0 2 31.099309521515725 31.0 31.0 34.0 27.0 34.0 3 31.194082055711217 31.0 31.0 34.0 27.0 34.0 4 34.72888469096894 37.0 35.0 37.0 32.0 37.0 5 34.824380243516785 37.0 35.0 37.0 32.0 37.0 6 34.93345307286168 37.0 35.0 37.0 32.0 37.0 7 34.85599552122064 37.0 35.0 37.0 32.0 37.0 8 34.8219545075128 37.0 35.0 37.0 31.0 37.0 9 36.34587279269544 39.0 37.0 39.0 31.0 39.0 10-11 36.32974507741861 39.0 37.0 39.0 31.5 39.0 12-13 36.27260493433751 39.0 37.0 39.0 31.0 39.0 14-15 37.413171101093894 40.0 37.0 41.0 31.0 41.0 16-17 37.39308356009063 40.0 37.0 41.0 31.0 41.0 18-19 37.408160702483876 40.0 37.0 41.0 31.0 41.0 20-21 37.368677109516526 40.0 37.0 41.0 31.0 41.0 22-23 37.22760195993756 40.0 37.0 41.0 30.5 41.0 24-25 37.14068716386417 40.0 37.0 41.0 30.0 41.0 26-27 36.98582898850864 40.0 36.0 41.0 30.0 41.0 28-29 36.823311143793205 39.5 36.0 41.0 30.0 41.0 30-31 36.65196313765173 39.0 36.0 41.0 30.0 41.0 32-33 36.497040623633644 39.0 35.5 41.0 29.0 41.0 34-35 36.33157728083927 39.0 35.0 41.0 28.5 41.0 36-37 36.15763080117467 39.0 35.0 40.0 28.0 41.0 38-39 36.03096380663908 39.0 35.0 40.0 27.0 41.0 40-41 36.00538140700234 39.0 35.0 40.0 27.0 41.0 42-43 35.95066504618573 39.0 35.0 40.0 27.0 41.0 44-45 35.76790544978789 39.0 35.0 40.0 26.5 41.0 46-47 35.71836171532938 39.0 35.0 40.0 26.0 41.0 48-49 35.69004583203365 39.0 35.0 40.0 26.0 41.0 50-51 34.74080741989836 38.0 34.0 39.5 25.0 40.5 52-53 34.961784855554654 38.0 33.5 39.5 25.0 40.5 54-55 35.55955072076145 39.0 34.0 40.0 26.0 41.0 56-57 35.528607327331535 39.0 34.0 41.0 26.0 41.0 58-59 35.37644302188255 38.5 34.0 40.0 26.0 41.0 60-61 35.20384145606141 38.0 34.0 40.0 26.0 41.0 62-63 34.96185741339713 38.0 34.0 40.0 25.0 41.0 64-65 34.69000089602535 37.5 33.5 40.0 25.0 41.0 66-67 34.390220388551626 37.0 33.0 40.0 24.0 41.0 68-69 34.11266144456803 36.5 33.0 39.5 24.0 41.0 70-71 33.77614039437242 36.0 32.5 39.0 23.5 41.0 72-73 33.42929578470599 35.5 32.0 39.0 23.0 40.5 74-75 33.06326700275477 35.0 32.0 38.0 22.5 40.0 76-77 32.719945659094016 35.0 32.0 37.0 22.0 39.0 78-79 32.39103462632711 35.0 31.0 37.0 22.0 39.0 80-81 32.04698440309553 35.0 31.0 36.0 21.5 39.0 82-83 31.738987477877266 35.0 31.0 36.0 20.5 37.5 84-85 31.460220314444264 34.5 31.0 35.5 20.0 37.0 86-87 31.154503807434047 34.0 30.0 35.0 20.0 37.0 88-89 30.91270954698171 34.0 30.0 35.0 19.5 36.0 90-91 30.670879714996566 34.0 30.0 35.0 19.0 36.0 92-93 30.51380141787591 34.0 29.5 35.0 18.5 36.0 94-95 30.344578069745808 34.0 29.0 35.0 18.0 35.0 96-97 30.2436435085658 34.0 29.0 35.0 18.0 35.0 98-99 30.070129074810033 34.0 29.0 35.0 18.0 35.0 100 28.661939470455753 33.0 26.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39706914989018927 1101 2 0.4088665638157032 1101 3 0.38127388956375086 1101 4 0.31134137778964543 1101 5 0.3270217044734878 1101 6 0.3724044791833734 1101 7 0.3906841232015168 1101 8 0.37268772817303386 1101 9 0.3593085259718336 1101 10-11 0.34425652473873214 1101 12-13 0.3533036840297683 1101 14-15 0.4199962766427561 1101 16-17 0.45466747303326116 1101 18-19 0.39458995082057413 1101 20-21 0.3662361757681154 1101 22-23 0.4156873163413053 1101 24-25 0.42138965411295715 1101 26-27 0.3677213653939333 1101 28-29 0.4216201290879269 1101 30-31 0.4972486473763169 1101 32-33 0.3423160728318919 1101 34-35 0.4023784024437944 1101 36-37 0.31397798979747904 1101 38-39 0.34065271073381354 1101 40-41 0.28956390423788747 1101 42-43 0.18425872628844786 1101 44-45 0.1681243520937059 1101 46-47 0.17579529451468545 1101 48-49 0.33073884787406627 1101 50-51 0.2841872275336854 1101 52-53 0.3890899616933936 1101 54-55 0.47417460618247986 1101 56-57 0.41963810448607575 1101 58-59 0.3942589279004025 1101 60-61 0.37183494860097 1101 62-63 0.352530417360974 1101 64-65 0.3407113009761602 1101 66-67 0.31035256572270953 1101 68-69 0.2866062754056884 1101 70-71 0.3559079641855831 1101 72-73 0.375604295316613 1101 74-75 0.19620198835765024 1101 76-77 0.20822794057011862 1101 78-79 0.10591855901584779 1101 80-81 0.1299323051784853 1101 82-83 0.13181438406517287 1101 84-85 -0.03238061348144683 1101 86-87 -0.05369873199492403 1101 88-89 -0.013438476617167794 1101 90-91 -0.09324429357235431 1101 92-93 -0.17184736893703345 1101 94-95 -0.23137842977877554 1101 96-97 -0.21003132136576852 1101 98-99 -0.28221206099669516 1101 100 -0.21096937295164864 1102 1 0.44955738406624235 1102 2 0.45558139058510605 1102 3 0.4190121066941792 1102 4 0.369255693957534 1102 5 0.41336959937863327 1102 6 0.4528005051425197 1102 7 0.4487143785703154 1102 8 0.45014639126622313 1102 9 0.4937128360322589 1102 10-11 0.48034397263014483 1102 12-13 0.5481699399318529 1102 14-15 0.5846553257849649 1102 16-17 0.5952412497545367 1102 18-19 0.5712576607325559 1102 20-21 0.5559858996724287 1102 22-23 0.6373774432403678 1102 24-25 0.6493259348893616 1102 26-27 0.5692058836961564 1102 28-29 0.5445432590381287 1102 30-31 0.5602230169813467 1102 32-33 0.5406122920616454 1102 34-35 0.5945831523175187 1102 36-37 0.5713848457721511 1102 38-39 0.5355677366656053 1102 40-41 0.6383475342422926 1102 42-43 0.6157193039925417 1102 44-45 0.54990552506721 1102 46-47 0.5011088073450836 1102 48-49 0.6298092497063976 1102 50-51 0.5682290991157828 1102 52-53 0.6250160805269545 1102 54-55 0.6844824827376996 1102 56-57 0.7717393414555787 1102 58-59 0.7521813868826897 1102 60-61 0.6837732409782333 1102 62-63 0.6268166906315145 1102 64-65 0.7320666141661789 1102 66-67 0.5921070664552772 1102 68-69 0.5510988926596809 1102 70-71 0.5974348120941642 1102 72-73 0.5893706024786312 1102 74-75 0.544623563500771 1102 76-77 0.5845718487533418 1102 78-79 0.5407132247413742 1102 80-81 0.5398246303913439 1102 82-83 0.5057078407311444 1102 84-85 0.35097195619534816 1102 86-87 0.33119747643564423 1102 88-89 0.293327239485091 1102 90-91 0.2071604875408326 1102 92-93 0.2339971017156124 1102 94-95 0.20548974391896735 1102 96-97 0.04063198867597961 1102 98-99 0.06716578455503708 1102 100 0.24445104164137277 1103 1 0.38064710681983627 1103 2 0.42933734627579767 1103 3 0.35189134385245424 1103 4 0.39945896993849317 1103 5 0.364807295273863 1103 6 0.34302238826844444 1103 7 0.3844390584527275 1103 8 0.3861051590397224 1103 9 0.41334788518648935 1103 10-11 0.4176704224061396 1103 12-13 0.39985001726247305 1103 14-15 0.509180934769482 1103 16-17 0.490972971691896 1103 18-19 0.44999688271310845 1103 20-21 0.4495724937026111 1103 22-23 0.5610665426076125 1103 24-25 0.4464865399516782 1103 26-27 0.49064612956780707 1103 28-29 0.5081282253160211 1103 30-31 0.4732392507148617 1103 32-33 0.4670614010783254 1103 34-35 0.5290214111719038 1103 36-37 0.5068566035099309 1103 38-39 0.5195903234735724 1103 40-41 0.45873386959946316 1103 42-43 0.41385789719466715 1103 44-45 0.40106856449725825 1103 46-47 0.38545596525973735 1103 48-49 0.4223098509672454 1103 50-51 0.42951213405881816 1103 52-53 0.46571087016051393 1103 54-55 0.5011386016595267 1103 56-57 0.5050512500339934 1103 58-59 0.4938198788568684 1103 60-61 0.4398385460402423 1103 62-63 0.425250707930374 1103 64-65 0.4470588938811133 1103 66-67 0.39358874318198644 1103 68-69 0.3171367555740119 1103 70-71 0.33389927359548466 1103 72-73 0.3336521586548571 1103 74-75 0.35303449367734885 1103 76-77 0.37734652815452563 1103 78-79 0.32440021849956935 1103 80-81 0.3055086316631517 1103 82-83 0.2784827590606973 1103 84-85 0.24894890126788383 1103 86-87 0.1815625542033139 1103 88-89 0.27047013148423105 1103 90-91 0.16228404052560563 1103 92-93 0.06402062982819601 1103 94-95 0.061649883000608696 1103 96-97 0.07670259547130698 1103 98-99 0.10260938026436506 1103 100 0.11122937988301018 1104 1 0.42234013260362957 1104 2 0.44967905962781884 1104 3 0.392337089563636 1104 4 0.39826975260887565 1104 5 0.3793473092260271 1104 6 0.32681856461020686 1104 7 0.39767065115224653 1104 8 0.4336105304847919 1104 9 0.45499922380208346 1104 10-11 0.47520741538541955 1104 12-13 0.4009390998762683 1104 14-15 0.4013240879065023 1104 16-17 0.5401335374949383 1104 18-19 0.47296642688039015 1104 20-21 0.4231549717862819 1104 22-23 0.5293951334376672 1104 24-25 0.5054685812051289 1104 26-27 0.48340417057361407 1104 28-29 0.5576215829895261 1104 30-31 0.46723251458156057 1104 32-33 0.4540288806405002 1104 34-35 0.4221757667412973 1104 36-37 0.4359949806229153 1104 38-39 0.4743882642728323 1104 40-41 0.42016201554405797 1104 42-43 0.2873326812893495 1104 44-45 0.3991913378680252 1104 46-47 0.4253903911303709 1104 48-49 0.4158459455559935 1104 50-51 0.45512804379767857 1104 52-53 0.5732761206264243 1104 54-55 0.5628294964807949 1104 56-57 0.5672948098154436 1104 58-59 0.6179200987427862 1104 60-61 0.6182264472354078 1104 62-63 0.6479147856314924 1104 64-65 0.5254081948963218 1104 66-67 0.5042501744533965 1104 68-69 0.40101873479695627 1104 70-71 0.3553960635552684 1104 72-73 0.4793271983282139 1104 74-75 0.4551710700756857 1104 76-77 0.47014528576574577 1104 78-79 0.3716065326133702 1104 80-81 0.30997206607354855 1104 82-83 0.35750574609209806 1104 84-85 0.24167769712184395 1104 86-87 0.2848568367077142 1104 88-89 0.3662621774186192 1104 90-91 0.3134425247921442 1104 92-93 0.38244556291067155 1104 94-95 0.3669374577617326 1104 96-97 0.4039295282722719 1104 98-99 0.332469112135918 1104 100 0.3485332214474859 1105 1 0.47873076218297683 1105 2 0.4203005108954727 1105 3 0.4081236213522992 1105 4 0.3985295956690251 1105 5 0.306060812833465 1105 6 0.3402049130870637 1105 7 0.41151837857898954 1105 8 0.4480345050429051 1105 9 0.4749610408269618 1105 10-11 0.41202023165944013 1105 12-13 0.494332781058219 1105 14-15 0.5009308586451411 1105 16-17 0.5095131420716328 1105 18-19 0.45731405833384997 1105 20-21 0.4927432191211949 1105 22-23 0.5762660151845438 1105 24-25 0.5792761533697899 1105 26-27 0.5709480651995307 1105 28-29 0.567023937985148 1105 30-31 0.5904360895052037 1105 32-33 0.5563622791783374 1105 34-35 0.4842898264219073 1105 36-37 0.44778193445828407 1105 38-39 0.5597748081449154 1105 40-41 0.5945055698609778 1105 42-43 0.5297697268977615 1105 44-45 0.5202555111006504 1105 46-47 0.48859887800022506 1105 48-49 0.41191777096810966 1105 50-51 0.5295752268505112 1105 52-53 0.5305915612978893 1105 54-55 0.5619287765669441 1105 56-57 0.5541026197051409 1105 58-59 0.597783825849163 1105 60-61 0.6094344695104681 1105 62-63 0.6526900875930082 1105 64-65 0.6245341170085084 1105 66-67 0.5213176887290842 1105 68-69 0.47851611624913915 1105 70-71 0.547422391389027 1105 72-73 0.593184084360658 1105 74-75 0.6049188497285982 1105 76-77 0.5851497768147951 1105 78-79 0.5977602713784123 1105 80-81 0.5868055111829023 1105 82-83 0.497988528007955 1105 84-85 0.39004144906006033 1105 86-87 0.5002237562226206 1105 88-89 0.5186802785078868 1105 90-91 0.4614825387899373 1105 92-93 0.433008181996005 1105 94-95 0.3871466546345168 1105 96-97 0.4249839244677638 1105 98-99 0.4407940853676635 1105 100 0.5961843352039331 1106 1 0.45378768500917843 1106 2 0.4090381869351276 1106 3 0.3959398382677719 1106 4 0.3512448205114609 1106 5 0.3573665874774008 1106 6 0.3553566814922817 1106 7 0.34738412610737157 1106 8 0.3665522609566665 1106 9 0.47629028968107434 1106 10-11 0.47135168780145165 1106 12-13 0.48487829056864484 1106 14-15 0.5631088018688004 1106 16-17 0.523862220203462 1106 18-19 0.5584025133482626 1106 20-21 0.4584151073993752 1106 22-23 0.5981420250949725 1106 24-25 0.5885590675995473 1106 26-27 0.6295937386380501 1106 28-29 0.6348099647768777 1106 30-31 0.6289473307885984 1106 32-33 0.627583200599787 1106 34-35 0.6241924333157911 1106 36-37 0.6678159221836566 1106 38-39 0.6696399089774943 1106 40-41 0.6243393649783613 1106 42-43 0.5642393401109302 1106 44-45 0.5863572625762998 1106 46-47 0.6010854348435615 1106 48-49 0.6930947487975985 1106 50-51 0.6346327567632883 1106 52-53 0.7040812767570301 1106 54-55 0.704085924353592 1106 56-57 0.6710701404854191 1106 58-59 0.6444313219598925 1106 60-61 0.6031234029432326 1106 62-63 0.6873598142545632 1106 64-65 0.7212834185646884 1106 66-67 0.7555670145628355 1106 68-69 0.708737696372765 1106 70-71 0.6267847394974737 1106 72-73 0.5217192762832923 1106 74-75 0.6540050137636797 1106 76-77 0.6654063710903344 1106 78-79 0.5620440781402039 1106 80-81 0.5945319678625509 1106 82-83 0.6027588983225236 1106 84-85 0.5633004171823934 1106 86-87 0.671993883525996 1106 88-89 0.6547898082250221 1106 90-91 0.6946012284222718 1106 92-93 0.6382840585533849 1106 94-95 0.7233733037985708 1106 96-97 0.6078525756532436 1106 98-99 0.5639877085515295 1106 100 0.7171381826761234 1107 1 0.39876646077932776 1107 2 0.32873298828398845 1107 3 0.3748748845473848 1107 4 0.39747083498774316 1107 5 0.3716629451154816 1107 6 0.39026958333408146 1107 7 0.34029265324595315 1107 8 0.322016096199242 1107 9 0.21936564562516736 1107 10-11 0.3769140062479792 1107 12-13 0.3798196014252184 1107 14-15 0.5021107940690825 1107 16-17 0.46886228354846793 1107 18-19 0.4283235842588482 1107 20-21 0.4922012600359764 1107 22-23 0.5155120649073055 1107 24-25 0.5046309917063851 1107 26-27 0.5606135907177219 1107 28-29 0.5565699654298228 1107 30-31 0.4964123211029019 1107 32-33 0.5591427033610259 1107 34-35 0.5689133827501536 1107 36-37 0.6453391362992633 1107 38-39 0.6546466954198138 1107 40-41 0.6009166981391942 1107 42-43 0.574213680512834 1107 44-45 0.6166240826840479 1107 46-47 0.4695059905887504 1107 48-49 0.5351706668067493 1107 50-51 0.5045497772483216 1107 52-53 0.5228663342560154 1107 54-55 0.5708010986729448 1107 56-57 0.5978439893301157 1107 58-59 0.6257155541466162 1107 60-61 0.6252041912084181 1107 62-63 0.6244726027088134 1107 64-65 0.621149711885657 1107 66-67 0.6044844058597647 1107 68-69 0.5855219240522445 1107 70-71 0.6261928596444193 1107 72-73 0.5841147722588147 1107 74-75 0.6028955869799049 1107 76-77 0.5867488124602716 1107 78-79 0.5620140501733815 1107 80-81 0.5893712993583975 1107 82-83 0.5563608778050728 1107 84-85 0.4736488768146998 1107 86-87 0.5575281571915056 1107 88-89 0.4080436399275911 1107 90-91 0.5174371987349033 1107 92-93 0.5274810741754798 1107 94-95 0.6281478297038738 1107 96-97 0.5365131918977752 1107 98-99 0.5862035008500897 1107 100 0.770382823262679 1108 1 0.30832510411807235 1108 2 0.323625083979735 1108 3 0.32566574468037146 1108 4 0.2258374473026663 1108 5 0.30893562923763085 1108 6 0.27928994662956086 1108 7 0.2442682864761352 1108 8 0.215773763292205 1108 9 0.24706934825010762 1108 10-11 0.23407861526151663 1108 12-13 0.25168059940091325 1108 14-15 0.40579621721170867 1108 16-17 0.3705830969943307 1108 18-19 0.4109760195064638 1108 20-21 0.3974861985616869 1108 22-23 0.3916574654426199 1108 24-25 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-0.33692273284817276 2316 76-77 -0.30075668611906536 2316 78-79 -0.6826882686191347 2316 80-81 -0.31413475804790636 2316 82-83 -0.25588696728511806 2316 84-85 -0.24933940268669375 2316 86-87 -0.1261654320710015 2316 88-89 -0.21530599441082643 2316 90-91 -0.14245918556738957 2316 92-93 -0.10877431168499996 2316 94-95 -0.12555111027021937 2316 96-97 -0.2793190401036476 2316 98-99 -0.012255078889083393 2316 100 -0.3582836262080846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 16.0 1 73.0 2 104.0 3 113.0 4 132.0 5 143.0 6 234.0 7 1001.0 8 2109.0 9 3326.0 10 5724.0 11 10893.0 12 18122.0 13 23312.0 14 27205.0 15 30021.0 16 33289.0 17 37925.0 18 43892.0 19 50935.0 20 58285.0 21 68125.0 22 76602.0 23 80877.0 24 82098.0 25 84959.0 26 91653.0 27 103471.0 28 118828.0 29 138266.0 30 164379.0 31 199328.0 32 247915.0 33 318725.0 34 427018.0 35 600397.0 36 909313.0 37 1429761.0 38 1539869.0 39 392662.0 40 565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15861730429242 18.973816127265923 12.89119887642072 23.97636769202094 2 15.552018309631373 20.36862862420272 40.64954248177352 23.42981058439238 3 17.981748469503213 25.934428249046444 30.696169987477294 25.38765329397305 4 11.933030109224028 16.78687128116342 39.12104545598522 32.15905315362733 5 12.48811426265569 37.95183491524551 36.075482165025726 13.484568657073076 6 30.41605666805678 37.918564596073686 18.27509797593871 13.390280759930823 7 26.412047046293736 32.80550184056288 23.244384359709276 17.538066753434105 8 25.0032945719199 36.027592477757935 21.032951378586333 17.936161571735834 9 26.002652116363794 15.225549143182619 20.831510300902423 37.94028843955116 10-11 23.958589142952967 26.300122599970987 29.773406660202834 19.967881596873212 12-13 24.611749439350284 24.29290760000822 29.398406093993763 21.696936866647736 14-15 22.073368268293702 25.82054470264619 26.879260111419118 25.226826917641 16-17 21.57973020536711 28.571272644363628 27.712587110291103 22.13641003997816 18-19 21.356384252877675 27.830938361282143 29.445040433241065 21.367636952599113 20-21 22.108214430032653 27.15158805503864 29.491430357636073 21.248767157292637 22-23 21.86257081999747 27.06934452047436 29.186102329125124 21.881982330403048 24-25 21.653953796503878 27.13799112843714 29.36263335239747 21.845421722661513 26-27 21.569547475882946 27.333944082432126 29.283386750859037 21.813121690825888 28-29 21.57594679522645 27.251158581250966 29.39995026709535 21.77294435642724 30-31 21.888842530939215 27.216617492202026 29.3292934536376 21.565246523221155 32-33 21.589318435443996 27.353274947418242 29.245908231971985 21.811498385165777 34-35 21.85035145295049 27.41994053874156 29.22823205618288 21.50147595212507 36-37 21.860993006059044 27.30828993355921 29.1074991184377 21.723217941944043 38-39 21.689795081710383 27.5316175713018 29.11985611172419 21.658731235263627 40-41 21.84884425838217 27.339550264443304 29.2167796221376 21.59482585503693 42-43 21.65709104880394 27.379022276748127 29.028722944760105 21.935163729687826 44-45 21.727075405078153 27.565526426708516 28.931582232728754 21.775815935484573 46-47 21.726640229377736 27.503724692809712 28.718820227558346 22.05081485025421 48-49 21.697320673922793 27.57129677743471 28.742713597905954 21.988668950736535 50-51 21.75958189679307 27.74903592985158 28.56749751019152 21.923884663163832 52-53 21.747395690139417 27.83440395212512 28.483203791694024 21.934996566041438 54-55 21.707429495602952 27.779824118063278 28.571286094544963 21.941460291788808 56-57 21.76374859901042 27.872632569344646 28.373447810008933 21.990171021636005 58-59 21.6982195659131 27.76634018827221 28.547756183491984 21.987684062322707 60-61 21.890828215314876 27.869148635504317 28.479058657484984 21.760964491695827 62-63 21.696713457047164 27.852335285943315 28.61410123050558 21.836850026503942 64-65 21.648129196068037 27.656488209058082 28.590249183986028 22.105133410887852 66-67 21.634858002340813 27.75315648997627 28.41096030165216 22.201025206030753 68-69 21.61416111690469 27.79005642141015 28.48534205795788 22.11044040372728 70-71 21.653498622582106 27.574899330614127 28.521352639605013 22.25024940719875 72-73 21.553357633393492 27.821589670908587 28.340671360209157 22.284381335488764 74-75 21.6297565473515 27.84474544792943 28.297800826958674 22.227697177760394 76-77 21.549479434279704 27.73618012450612 28.381062611350888 22.333277829863288 78-79 21.586890388115602 27.609239802932933 28.35373290291733 22.450136906034135 80-81 21.55619396908782 27.844762680854352 28.14642891052379 22.452614439534038 82-83 21.669204186756538 27.807316480696038 28.221410110975935 22.302069221571493 84-85 21.63415669380385 27.754520511170668 28.18918174244937 22.422141052576112 86-87 21.45026455311682 27.778690468810225 28.375350064554734 22.39569491351822 88-89 21.552331045650604 27.568212036195757 28.384592142816523 22.494864775337124 90-91 21.739386515503565 27.645922039189653 28.287080774800526 22.32761067050626 92-93 21.624860624437563 27.57915716549764 28.302602759868627 22.493379450196173 94-95 21.480839138899363 27.487992875642608 28.37272232552912 22.658445659928912 96-97 21.548213431437947 27.406803986173717 28.413299374044055 22.631683208344285 98-99 21.514786076616627 27.32168076300332 28.356617517991335 22.806915642388724 100 21.66641774064317 27.283792920867405 28.26900078398218 22.78078855450725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2073.0 1 1494.0 2 1447.0 3 2085.0 4 1716.0 5 1554.0 6 2458.0 7 4591.0 8 7360.0 9 8765.5 10 7491.0 11 5941.5 12 6066.0 13 6492.0 14 6882.0 15 7272.5 16 7711.5 17 8412.0 18 9263.5 19 10377.0 20 12056.5 21 14266.0 22 17246.5 23 21784.0 24 27871.5 25 35412.5 26 44929.0 27 56443.5 28 68575.5 29 81877.0 30 97432.5 31 113282.0 32 130252.5 33 148319.0 34 168213.5 35 187814.5 36 209759.0 37 229241.0 38 240820.0 39 250801.5 40 255724.5 41 256725.0 42 258957.0 43 264731.0 44 270227.0 45 275975.0 46 278801.5 47 276626.5 48 276672.5 49 276876.0 50 272958.0 51 262111.0 52 246779.5 53 233300.5 54 219786.0 55 201545.5 56 181135.0 57 160009.5 58 139208.5 59 118686.0 60 97887.0 61 79483.0 62 63726.0 63 49006.5 64 35997.5 65 26457.0 66 19385.5 67 14325.5 68 10867.0 69 8102.5 70 6030.5 71 4559.0 72 3511.5 73 2663.0 74 2062.5 75 1512.5 76 1039.5 77 732.0 78 535.0 79 397.5 80 306.5 81 252.5 82 214.0 83 179.0 84 146.0 85 124.5 86 111.5 87 86.0 88 68.5 89 58.5 90 39.0 91 32.5 92 29.5 93 22.0 94 15.5 95 12.0 96 10.0 97 8.5 98 6.0 99 2.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16823718127940293 2 0.10593310261241917 3 0.031556261297161756 4 0.03381990429371307 5 0.02772962670775358 6 0.002694813091132515 7 0.003961375243964798 8 0.004944982022228165 9 0.005686055622289608 10-11 0.005046037513145635 12-13 0.007916013455201763 14-15 0.017576918386911833 16-17 0.008259602124321159 18-19 0.01648551908500316 20-21 0.017711659041468458 22-23 0.045623185632873486 24-25 0.014834946066684496 26-27 0.009748486357171874 28-29 0.02735235287499503 30-31 0.015454753077644975 32-33 0.02093196068537181 34-35 0.020743323768992537 36-37 0.02155176769633229 38-39 0.019719294794362182 40-41 0.025964524133061783 42-43 0.03451381866467969 44-45 0.03756569449038726 46-47 0.0214237640745035 48-49 0.013379746997472939 50-51 0.012308558793747764 52-53 0.02232652646003289 54-55 0.025802835347593835 56-57 0.019719294794362182 58-59 0.025998209296700943 60-61 0.0264630645549213 62-63 0.05256232934253972 64-65 0.03190658699900899 66-67 0.021497871434509642 68-69 0.02765551934774744 70-71 0.04141927721070676 72-73 0.03894678619959268 74-75 0.04090052569066375 76-77 0.023020440830999512 78-79 0.016802159623211233 80-81 0.023033914896455173 82-83 0.018358414183340263 84-85 0.018910850867022426 86-87 0.021625875056338437 88-89 0.01814956616877749 90-91 0.028693022387833455 92-93 0.0264630645549213 94-95 0.02003593533257025 96-97 0.013676176437497516 98-99 0.018277569790606286 100 0.0421603508107682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7421665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.1520536530045 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87576415774073 47.917103290523585 2 15.166870087312724 19.156379870042443 3 4.660290569381342 8.829207602284841 4 1.7572924962418148 4.439065200267412 5 0.8295275171264604 2.6193183134106914 6 0.47586459254258695 1.8031095767888745 7 0.29208674043414024 1.2912114252259619 8 0.18644807757428866 0.9419663198776818 9 0.13467088775065963 0.7654268815854662 >10 0.5693423422161005 6.070074440077994 >50 0.02866394841408369 1.2514178000451825 >100 0.02096884158218043 2.5966888998995628 >500 0.0014731644032382639 0.6544328523890257 >1k 6.481880060513786E-4 0.6522897234650066 >5k 0.0 0.0 >10k+ 8.838927352976073E-5 1.012307804116232 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22058 0.2972109358210051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20077 0.27051881215333756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12271 0.1653402572064355 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3474065455662576E-5 0.0 4 0.0 0.0 0.0 1.3474065455662576E-5 0.0 5 0.0 0.0 0.0 1.3474065455662576E-5 0.0 6 0.0 0.0 0.0 1.3474065455662576E-5 0.0 7 0.0 0.0 0.0 1.3474065455662576E-5 0.0 8 0.0 0.0 0.0 2.6948130911325153E-5 0.0 9 0.0 0.0 0.0 4.0422196366987734E-5 0.0 10-11 0.0 0.0 0.0 7.410736000614419E-5 0.0 12-13 0.0 0.0 0.0 1.0779252364530061E-4 0.0 14-15 0.0 0.0 0.0 1.0779252364530061E-4 2.0211098183493864E-5 16-17 0.0 0.0 0.0 1.0779252364530061E-4 5.3896261822650305E-5 18-19 0.0 0.0 0.0 1.0779252364530061E-4 5.3896261822650305E-5 20-21 0.0 0.0 0.0 1.2126658910096319E-4 5.3896261822650305E-5 22-23 0.0 0.0 0.0 1.8189988365144478E-4 6.0633294550481594E-5 24-25 0.0 0.0 0.0 3.0316647275240797E-4 8.758142546180675E-5 26-27 0.0 0.0 0.0 4.513811927646963E-4 1.0105549091746933E-4 28-29 6.737032727831288E-6 0.0 0.0 0.0011116104000921626 1.0779252364530061E-4 30-31 1.3474065455662576E-5 0.0 0.0 0.00449360082946347 1.2126658910096319E-4 32-33 1.3474065455662576E-5 0.0 0.0 0.012429825382848727 1.4821472001228834E-4 34-35 1.3474065455662576E-5 0.0 0.0 0.024745121209324324 1.6168878546795094E-4 36-37 1.3474065455662576E-5 0.0 0.0 0.04530654509466542 1.6168878546795094E-4 38-39 2.0211098183493864E-5 0.0 0.0 0.07828432029739957 1.6168878546795094E-4 40-41 2.6948130911325153E-5 0.0 0.0 0.11495398943498528 2.0211098183493866E-4 42-43 2.6948130911325153E-5 0.0 0.0 0.14995961148879666 2.1558504729060122E-4 44-45 2.6948130911325153E-5 0.0 0.0 0.19602070424897916 2.1558504729060122E-4 46-47 2.6948130911325153E-5 0.0 0.0 0.25355496374465836 2.1558504729060122E-4 48-49 2.6948130911325153E-5 6.737032727831288E-6 0.0 0.3159196757061926 2.1558504729060122E-4 50-51 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.3809994118570429 2.1558504729060122E-4 52-53 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.4466585328224866 2.290591127462638E-4 54-55 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.5241478832580021 2.4253317820192638E-4 56-57 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.6159669023056147 2.4253317820192638E-4 58-59 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.7227488710417407 2.4253317820192638E-4 60-61 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.8371841089566829 2.4253317820192638E-4 62-63 2.6948130911325153E-5 1.3474065455662576E-5 0.0 0.9541187860136506 2.4253317820192638E-4 64-65 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.0751967381982346 2.4927021092975766E-4 66-67 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.2210265486248706 2.5600724365758894E-4 68-69 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.3907054549080293 2.5600724365758894E-4 70-71 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.576405025017971 2.5600724365758894E-4 72-73 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.7668137810046667 3.0990350548023925E-4 74-75 2.6948130911325153E-5 1.3474065455662576E-5 0.0 1.9580175068532466 3.0990350548023925E-4 76-77 2.6948130911325153E-5 1.3474065455662576E-5 0.0 2.172174303205548 3.2337757093590187E-4 78-79 2.6948130911325153E-5 1.3474065455662576E-5 0.0 2.4238765829500526 3.2337757093590187E-4 80-81 3.3685163639156445E-5 1.3474065455662576E-5 0.0 2.696665775132669 3.3011460366373315E-4 82-83 4.0422196366987734E-5 1.3474065455662576E-5 0.0 2.9796211497015834 3.3685163639156444E-4 84-85 4.0422196366987734E-5 1.3474065455662576E-5 0.0 3.2627449500886927 3.3685163639156444E-4 86-87 4.0422196366987734E-5 1.3474065455662576E-5 0.0 3.5606228521497534 3.3685163639156444E-4 88 4.0422196366987734E-5 1.3474065455662576E-5 0.0 3.8068816094501705 3.3685163639156444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11465 0.0 50.029243 1 GTATCAA 19835 0.0 37.79737 1 TCAACGC 23745 0.0 30.591686 4 ATCAACG 23875 0.0 30.426138 3 CAACGCA 23930 0.0 30.353954 5 AACGCAG 24870 0.0 29.25823 6 TATCAAC 25435 0.0 28.76802 2 ACGCAGA 28075 0.0 25.651304 7 GTACATG 22535 0.0 25.411293 1 CGCAGAG 28625 0.0 24.979095 8 TACCTGG 5970 0.0 24.891146 2 TACATGG 23195 0.0 24.04487 2 ACATGGG 22860 0.0 23.493664 3 GTACCTG 6460 0.0 23.473297 1 GCAGAGT 31715 0.0 21.955076 9 CATGGGG 12990 0.0 21.59391 4 GAGTACT 19875 0.0 20.98141 12-13 CAGAGTA 30955 0.0 19.822412 10-11 AGAGTAC 29225 0.0 19.420326 10-11 ACCTGGG 7455 0.0 19.286549 3 >>END_MODULE