FastQCFastQC Report
Mon 6 Jun 2016
ERR1211509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7421665
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240240.3237009485068378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224870.3029913099014844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149440.20135643416942156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124950.048.9682581
GTATCAA209050.035.8376271
TCAACGC255800.028.7508584
ATCAACG257750.028.4421833
CAACGCA260750.028.2228935
AACGCAG270100.027.2101926
TATCAAC271000.027.1585752
TACCTGG57850.025.363732
GTACATG236350.024.8729921
GTACCTG62700.024.302511
ACGCAGA307400.023.8470337
TACATGG241400.023.7480722
CGCAGAG312950.023.2889778
ACATGGG238050.023.1364353
CATGGGG127500.022.2619784
TATAACG7650.020.9015942
GCAGAGT348900.020.404339
GAGTACT219800.020.08617812-13
ACCTGGG72400.019.6671333
TAACGCA7550.019.295314
Produced by FastQC (version 0.11.5)