##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7421665 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11113988572645 33.0 31.0 34.0 30.0 34.0 2 32.329498434650446 34.0 31.0 34.0 30.0 34.0 3 32.34228949972816 34.0 31.0 34.0 30.0 34.0 4 35.98070742885862 37.0 35.0 37.0 35.0 37.0 5 35.902897530405916 37.0 35.0 37.0 35.0 37.0 6 35.91730319274718 37.0 35.0 37.0 35.0 37.0 7 35.83845323118195 37.0 35.0 37.0 35.0 37.0 8 35.83334453926444 37.0 35.0 37.0 35.0 37.0 9 37.48209990615313 39.0 37.0 39.0 35.0 39.0 10-11 37.44489087017536 39.0 37.0 39.0 34.0 39.0 12-13 37.367086900311456 39.0 37.0 39.0 34.0 39.0 14-15 38.604688840037916 40.0 38.0 41.0 34.0 41.0 16-17 38.53546704142534 40.0 38.0 41.0 34.0 41.0 18-19 38.52416283947066 40.0 38.0 41.0 34.0 41.0 20-21 38.46701467932061 40.0 38.0 41.0 34.0 41.0 22-23 38.3673838687141 40.0 38.0 41.0 34.0 41.0 24-25 38.262334462684585 40.0 38.0 41.0 33.5 41.0 26-27 38.093309789649624 40.0 38.0 41.0 33.0 41.0 28-29 38.12901505255222 40.0 38.0 41.0 33.0 41.0 30-31 38.05568912097218 40.0 37.5 41.0 33.0 41.0 32-33 37.88654075601634 40.0 37.0 41.0 33.0 41.0 34-35 37.77857792018368 40.0 37.0 41.0 33.0 41.0 36-37 37.725144425139106 40.0 37.0 41.0 33.0 41.0 38-39 37.63539064347421 40.0 37.0 41.0 32.5 41.0 40-41 37.48168807134248 40.0 37.0 41.0 32.0 41.0 42-43 37.336089475879064 40.0 36.0 41.0 31.0 41.0 44-45 37.160573537070185 40.0 36.0 41.0 31.0 41.0 46-47 36.9162496555692 39.0 36.0 41.0 30.0 41.0 48-49 36.704438357161095 39.0 36.0 41.0 30.0 41.0 50-51 36.512783452230735 39.0 35.0 41.0 29.5 41.0 52-53 36.322180669701474 39.0 35.0 40.0 29.0 41.0 54-55 36.134672610526074 39.0 35.0 40.0 28.0 41.0 56-57 35.943393699392246 38.5 35.0 40.0 28.0 41.0 58-59 35.691991352344786 38.0 34.0 40.0 28.0 41.0 60-61 35.3914986731414 38.0 34.0 40.0 27.0 41.0 62-63 35.0815083273093 37.5 34.0 40.0 26.0 41.0 64-65 34.77572734689588 37.0 33.5 40.0 26.0 41.0 66-67 34.44133459270932 36.5 33.0 39.5 26.0 41.0 68-69 34.04520468115982 36.0 32.5 39.0 25.5 40.5 70-71 33.6611707750215 35.5 32.0 39.0 25.0 40.0 72-73 33.256562914116984 35.0 32.0 38.0 24.0 40.0 74-75 32.84907517383229 35.0 31.0 37.0 23.5 39.5 76-77 31.293599279945944 33.5 29.5 35.5 21.5 38.5 78-79 32.12229991518076 34.5 31.0 36.0 23.0 39.0 80-81 32.086330156373265 35.0 31.0 36.0 23.0 38.0 82-83 31.79219083588386 34.0 31.0 35.5 22.5 37.0 84-85 31.4820064635092 34.0 31.0 35.0 21.5 37.0 86-87 31.18357646161609 34.0 30.0 35.0 20.0 36.5 88-89 30.898217381140217 34.0 30.0 35.0 20.0 36.0 90-91 30.61266245512294 34.0 30.0 35.0 19.5 36.0 92-93 30.39951412519967 34.0 29.0 35.0 19.0 35.0 94-95 30.177133904588793 34.0 29.0 35.0 18.5 35.0 96-97 29.973613333396212 34.0 29.0 35.0 18.0 35.0 98-99 29.810949699292543 34.0 29.0 35.0 18.0 35.0 100 27.9525367959885 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29581021282740494 1101 2 0.2151732388405918 1101 3 0.3169019045374952 1101 4 0.2191981880074465 1101 5 0.18300897081304157 1101 6 0.17243996971841113 1101 7 0.20816895874788344 1101 8 0.20501939266250702 1101 9 0.22275827764357103 1101 10-11 0.2264690953588655 1101 12-13 0.22705293003627247 1101 14-15 0.3094555714596865 1101 16-17 0.2829782222332611 1101 18-19 0.3218632150574905 1101 20-21 0.30674834336203816 1101 22-23 0.2736361367029687 1101 24-25 0.27821692816545607 1101 26-27 0.2660831292156445 1101 28-29 0.32461882973616696 1101 30-31 0.3169580272220429 1101 32-33 0.37582717200918125 1101 34-35 0.3473646458416013 1101 36-37 0.3810175590442313 1101 38-39 0.3928212372308266 1101 40-41 0.3974472979087338 1101 42-43 0.40856831110396996 1101 44-45 0.35442831187278756 1101 46-47 0.36736009162743244 1101 48-49 0.3447033329050271 1101 50-51 0.38380703841313846 1101 52-53 0.37008716578133516 1101 54-55 0.3015282473440877 1101 56-57 0.2991281855371142 1101 58-59 0.4114653642427655 1101 60-61 0.37206858802385767 1101 62-63 0.3478675088480756 1101 64-65 0.30194485947981065 1101 66-67 0.25820629693202335 1101 68-69 0.31834554555560857 1101 70-71 0.2185603746974465 1101 72-73 0.2173351482179129 1101 74-75 0.19691720518644473 1101 76-77 0.2676409523308827 1101 78-79 0.321994899149459 1101 80-81 0.36150678226936606 1101 82-83 0.3326837666262392 1101 84-85 0.2716867605226341 1101 86-87 0.20006197483751365 1101 88-89 0.2136835729729505 1101 90-91 0.17480659794236075 1101 92-93 0.16634574143746406 1101 94-95 0.158970392980617 1101 96-97 0.10478134273173367 1101 98-99 -0.03257283168884939 1101 100 0.016157872025146958 1102 1 0.344166544122551 1102 2 0.30208900276469564 1102 3 0.354716381839026 1102 4 0.2445196771769247 1102 5 0.2095747978312943 1102 6 0.2109571176169709 1102 7 0.23128711091240461 1102 8 0.2428656305592085 1102 9 0.25801249486143263 1102 10-11 0.2681635260067736 1102 12-13 0.30772903262038653 1102 14-15 0.40829340727479035 1102 16-17 0.40782203581506593 1102 18-19 0.4320826063629397 1102 20-21 0.4181963514559257 1102 22-23 0.36204871638854996 1102 24-25 0.3316814894067406 1102 26-27 0.3514805369199152 1102 28-29 0.4279896074697618 1102 30-31 0.40234577211990086 1102 32-33 0.481916346901059 1102 34-35 0.5043703614458863 1102 36-37 0.46202856653692237 1102 38-39 0.491631517761995 1102 40-41 0.5425548344489783 1102 42-43 0.44716069860755425 1102 44-45 0.4948578847796412 1102 46-47 0.5131153474341303 1102 48-49 0.5615119475249415 1102 50-51 0.5842812324952718 1102 52-53 0.5649512502662617 1102 54-55 0.5514997221605 1102 56-57 0.48341154042297063 1102 58-59 0.6049807525696025 1102 60-61 0.5674237385023488 1102 62-63 0.5987376825657904 1102 64-65 0.5169634518774515 1102 66-67 0.5209595800786033 1102 68-69 0.5600909610787284 1102 70-71 0.5265885816960321 1102 72-73 0.5143534667274636 1102 74-75 0.4471550115571148 1102 76-77 0.5452211496432184 1102 78-79 0.5541728768970628 1102 80-81 0.4597305268319545 1102 82-83 0.45723244842401556 1102 84-85 0.3692093617182728 1102 86-87 0.37349907923244885 1102 88-89 0.42283050133688604 1102 90-91 0.4308292333740127 1102 92-93 0.3238681602340634 1102 94-95 0.35452697859214055 1102 96-97 0.38263358242550183 1102 98-99 0.3887210188142909 1102 100 0.3826287588941675 1103 1 0.30758093974549183 1103 2 0.2640666340034912 1103 3 0.31511154178623 1103 4 0.18061292306866505 1103 5 0.18261562880447002 1103 6 0.21571267869592248 1103 7 0.1761795202565395 1103 8 0.18818456503885983 1103 9 0.2333590409055759 1103 10-11 0.20886286203354842 1103 12-13 0.29077781745995424 1103 14-15 0.36737413050561685 1103 16-17 0.3529042581314741 1103 18-19 0.36009115217507315 1103 20-21 0.3297503660688861 1103 22-23 0.3212369011019618 1103 24-25 0.34758154637243166 1103 26-27 0.3323549047236014 1103 28-29 0.41656496877082816 1103 30-31 0.48706940685250544 1103 32-33 0.4673664814804823 1103 34-35 0.4665742921862588 1103 36-37 0.49780844196885 1103 38-39 0.5054646473167281 1103 40-41 0.5086188021875131 1103 42-43 0.533480805440341 1103 44-45 0.445294536932181 1103 46-47 0.5161569562612769 1103 48-49 0.5289382431879162 1103 50-51 0.5238573730011424 1103 52-53 0.5106168841657421 1103 54-55 0.5700094946686036 1103 56-57 0.6042604062111394 1103 58-59 0.625315548065771 1103 60-61 0.677519976261344 1103 62-63 0.6410846900315548 1103 64-65 0.6547743324447239 1103 66-67 0.5926466046245125 1103 68-69 0.5059652351684605 1103 70-71 0.5637562327532208 1103 72-73 0.5857121476875093 1103 74-75 0.5366219513844896 1103 76-77 0.5339230318190538 1103 78-79 0.6311158260926746 1103 80-81 0.4871803925395781 1103 82-83 0.5869554699352761 1103 84-85 0.4991223087711276 1103 86-87 0.42524676428669395 1103 88-89 0.4628771337385764 1103 90-91 0.44510653330442906 1103 92-93 0.3873040087542634 1103 94-95 0.4818978290831488 1103 96-97 0.5142714079893302 1103 98-99 0.5065130929889143 1103 100 0.5626998278428168 1104 1 0.2758231419362076 1104 2 0.2534256743383665 1104 3 0.29725857375391485 1104 4 0.0947371000357009 1104 5 0.1598449431185358 1104 6 0.17377543024057474 1104 7 0.1947191080161943 1104 8 0.18183464731009025 1104 9 0.24558073523284918 1104 10-11 0.24465052632588424 1104 12-13 0.25786528509147644 1104 14-15 0.34739616441870425 1104 16-17 0.2922851938497786 1104 18-19 0.32008711942255275 1104 20-21 0.21515347371686033 1104 22-23 0.2982566465391585 1104 24-25 0.33136431868627625 1104 26-27 0.37983173649197965 1104 28-29 0.3958283817347805 1104 30-31 0.3955954460687181 1104 32-33 0.3987946385416592 1104 34-35 0.5033946865921948 1104 36-37 0.364927365241023 1104 38-39 0.4609784316171428 1104 40-41 0.5538153458552273 1104 42-43 0.586582176832728 1104 44-45 0.4730334138123098 1104 46-47 0.48621988293478324 1104 48-49 0.5556854144035697 1104 50-51 0.4850714606815245 1104 52-53 0.47366905529331405 1104 54-55 0.5496589735661814 1104 56-57 0.6098416339362913 1104 58-59 0.5877565853075026 1104 60-61 0.6110661407596822 1104 62-63 0.5693654658934619 1104 64-65 0.6592881548835621 1104 66-67 0.6233520045280159 1104 68-69 0.6713941349880841 1104 70-71 0.5800206631406155 1104 72-73 0.6349790050804529 1104 74-75 0.6352636252469992 1104 76-77 0.5642154935989758 1104 78-79 0.6165440657585748 1104 80-81 0.7749449692382413 1104 82-83 0.5940118462078487 1104 84-85 0.5771495069333668 1104 86-87 0.6630553626349744 1104 88-89 0.7336125449827087 1104 90-91 0.6183445727641761 1104 92-93 0.6152566275036868 1104 94-95 0.6686524304486419 1104 96-97 0.5783537780711612 1104 98-99 0.5783692220629923 1104 100 0.7764503766169746 1105 1 0.2615958140217032 1105 2 0.1954743275467763 1105 3 0.24272802585321074 1105 4 0.1743744506806877 1105 5 0.13628079938872872 1105 6 0.174898225243318 1105 7 0.22065476894208302 1105 8 0.172476644761538 1105 9 0.24250867346351157 1105 10-11 0.22769334021741372 1105 12-13 0.25018047424323697 1105 14-15 0.3145873293297612 1105 16-17 0.3451561555416447 1105 18-19 0.35924361474748423 1105 20-21 0.2513954617739813 1105 22-23 0.32549721961061095 1105 24-25 0.3158133292403633 1105 26-27 0.3347084473843509 1105 28-29 0.3370852098907662 1105 30-31 0.3863393973982525 1105 32-33 0.4188254143088841 1105 34-35 0.43141431738106206 1105 36-37 0.4454827664312475 1105 38-39 0.4278479248763247 1105 40-41 0.43777287949306043 1105 42-43 0.4355351527764313 1105 44-45 0.435686768982535 1105 46-47 0.46299394116520887 1105 48-49 0.5409526024646851 1105 50-51 0.4948712488171054 1105 52-53 0.48701822512769866 1105 54-55 0.5031438863348257 1105 56-57 0.5288847325099937 1105 58-59 0.5718515547810767 1105 60-61 0.658583661686059 1105 62-63 0.615531954240879 1105 64-65 0.571039241151091 1105 66-67 0.578207084871643 1105 68-69 0.6008771346063568 1105 70-71 0.6762369308527951 1105 72-73 0.6079379379885168 1105 74-75 0.5842498931735278 1105 76-77 0.4980863515253269 1105 78-79 0.5912504021386198 1105 80-81 0.6395899515453536 1105 82-83 0.6355973874032372 1105 84-85 0.6686650918928905 1105 86-87 0.655285535654194 1105 88-89 0.6887887513034237 1105 90-91 0.7687206132696041 1105 92-93 0.751885221057762 1105 94-95 0.754239025975032 1105 96-97 0.7169557353906306 1105 98-99 0.7483862446427665 1105 100 0.9113188924925701 1106 1 0.2297771798246444 1106 2 0.1607099109769763 1106 3 0.2138311612979038 1106 4 0.1339417347943197 1106 5 0.15171248090316425 1106 6 0.11750865158288093 1106 7 0.1275348842643993 1106 8 0.13868080052953502 1106 9 0.1592932163508678 1106 10-11 0.20415912763753852 1106 12-13 0.20197393833098687 1106 14-15 0.26961641327880415 1106 16-17 0.30289700073210213 1106 18-19 0.2916304148786324 1106 20-21 0.31641234395981854 1106 22-23 0.29197776456071267 1106 24-25 0.36870216377364073 1106 26-27 0.3351822353402838 1106 28-29 0.3550724301152357 1106 30-31 0.3715489308408948 1106 32-33 0.37598734963377467 1106 34-35 0.3826488380713542 1106 36-37 0.4043648711023877 1106 38-39 0.3913420997585675 1106 40-41 0.37567976065305686 1106 42-43 0.4000664878307205 1106 44-45 0.49446944152533234 1106 46-47 0.4708314420119848 1106 48-49 0.5061555141613354 1106 50-51 0.5332971801652917 1106 52-53 0.5852679986694014 1106 54-55 0.4359989347201818 1106 56-57 0.43590257526392406 1106 58-59 0.5588740567699233 1106 60-61 0.584096869070656 1106 62-63 0.5797884009087468 1106 64-65 0.6234854586663019 1106 66-67 0.6488504648000699 1106 68-69 0.6003596175839547 1106 70-71 0.6736176377534662 1106 72-73 0.5949190724570386 1106 74-75 0.5929041211474555 1106 76-77 0.6286258104749471 1106 78-79 0.7157288020691297 1106 80-81 0.7215709600647457 1106 82-83 0.678165272070558 1106 84-85 0.6917469175690556 1106 86-87 0.5574858904658377 1106 88-89 0.616230017664229 1106 90-91 0.6545775605440589 1106 92-93 0.663132107421692 1106 94-95 0.7417216616218028 1106 96-97 0.7987725024212935 1106 98-99 0.7331134561932195 1106 100 0.6425309904124852 1107 1 0.19346094257617352 1107 2 0.166014445723782 1107 3 0.25582237973168986 1107 4 0.16111448660105054 1107 5 0.11726589572253232 1107 6 0.14637003493537293 1107 7 0.18795383348290784 1107 8 0.16323338382395036 1107 9 0.19974624761625392 1107 10-11 0.14311909286600155 1107 12-13 0.22239499620712166 1107 14-15 0.2870460339372656 1107 16-17 0.24525603435050414 1107 18-19 0.21293728559416536 1107 20-21 0.25458485164465827 1107 22-23 0.2842998936251746 1107 24-25 0.3283466472645884 1107 26-27 0.3461414382360317 1107 28-29 0.36514242755950477 1107 30-31 0.24992887391322682 1107 32-33 0.3155989156513215 1107 34-35 0.3023878895579344 1107 36-37 0.38335729333482504 1107 38-39 0.35141720743897764 1107 40-41 0.31842382833280425 1107 42-43 0.3419254918475261 1107 44-45 0.38246999798681003 1107 46-47 0.390163501867157 1107 48-49 0.3819843286815754 1107 50-51 0.36064690815942413 1107 52-53 0.45362992983433514 1107 54-55 0.46083154555505956 1107 56-57 0.484696072266118 1107 58-59 0.5891229088245069 1107 60-61 0.5222786044167052 1107 62-63 0.5359423921189048 1107 64-65 0.6339510273691857 1107 66-67 0.6299516360247779 1107 68-69 0.5316087602946382 1107 70-71 0.6448165235148906 1107 72-73 0.5936328948478931 1107 74-75 0.5923326456937588 1107 76-77 0.5064385112450971 1107 78-79 0.6107027599646813 1107 80-81 0.6323046973930104 1107 82-83 0.6043966684355766 1107 84-85 0.47891033329717914 1107 86-87 0.40839158389016106 1107 88-89 0.42255267712126 1107 90-91 0.3329362221324068 1107 92-93 0.48323239595817213 1107 94-95 0.6875526103812959 1107 96-97 0.717574321982088 1107 98-99 0.7747163514983058 1107 100 0.8211473869736317 1108 1 0.26075502758532565 1108 2 0.20608896856382586 1108 3 0.25074283794044305 1108 4 0.14551321660772487 1108 5 0.14999843821371428 1108 6 0.16765490981399012 1108 7 0.16571613998179657 1108 8 0.20638782386523502 1108 9 0.16974868742165228 1108 10-11 0.20604966592900098 1108 12-13 0.18479902438516405 1108 14-15 0.23571181817468556 1108 16-17 0.2700704014781081 1108 18-19 0.28030369276406475 1108 20-21 0.3253810777313717 1108 22-23 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2316 68-69 -0.034314042721938165 2316 70-71 -0.2449068296666539 2316 72-73 -0.29809468848245757 2316 74-75 -0.42741244970275005 2316 76-77 -0.3571029503863734 2316 78-79 -0.17168289463371877 2316 80-81 -0.0896291638685831 2316 82-83 0.013292594105450917 2316 84-85 -0.09985015359656657 2316 86-87 -0.06350463169745879 2316 88-89 -0.05666393733112329 2316 90-91 0.027533490182854337 2316 92-93 0.13807115385616697 2316 94-95 0.15960653540540193 2316 96-97 0.287341080407554 2316 98-99 0.11785789793960433 2316 100 -0.06817135803482444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 14.0 11 29.0 12 73.0 13 229.0 14 686.0 15 1836.0 16 4227.0 17 8615.0 18 15099.0 19 23609.0 20 33556.0 21 45415.0 22 58784.0 23 66753.0 24 71923.0 25 79991.0 26 93229.0 27 112581.0 28 136632.0 29 164832.0 30 197817.0 31 240266.0 32 292761.0 33 366263.0 34 475325.0 35 643865.0 36 944894.0 37 1491704.0 38 1579389.0 39 271224.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.47938095363216 20.548183870596105 14.014227744978552 23.958207430793184 2 14.850484035661571 21.506805299913072 40.024787976105706 23.617922688319652 3 16.95475802804892 26.845072844091384 31.233800671660735 24.966368456198957 4 11.733006719566239 17.862889588647207 38.770226406399686 31.633877285386863 5 12.18600182711405 38.52893065320674 36.05391872845797 13.231148791221228 6 30.31366387360581 38.7363006421655 17.859474369903985 13.0905611143247 7 26.20515746803446 33.20889584749514 23.114840133581886 17.471106550888514 8 25.116494405991375 36.782858577280024 20.536377528244916 17.56426948848368 9 25.90831033198076 15.278000286997596 20.673972754092244 38.139716626929406 10-11 24.012455426107216 26.799592813741928 29.538984850434506 19.64896690971635 12-13 24.66460693119401 24.627714939976407 29.30562077377516 21.402057355054424 14-15 22.006544353591813 26.139343395316278 26.808916867037247 25.045195384054658 16-17 21.5543416682482 28.973666972462787 27.56540813272158 21.906583226567438 18-19 21.365801332586052 28.115828904191552 29.45374400843917 21.06462575478323 20-21 22.120263679783374 27.383436821980034 29.463232537789906 21.033066960446686 22-23 21.90143464447901 27.307054906618323 29.20302446978033 21.58848597912234 24-25 21.726015774493714 27.42420517661503 29.333332165580916 21.51644688331034 26-27 21.680072932529594 27.5538579405615 29.318875549843742 21.447193577065164 28-29 21.65247295798021 27.468855090237977 29.44976944318461 21.428902508597204 30-31 21.94647509133532 27.427315087918245 29.37937256806324 21.2468372526832 32-33 21.71047786977916 27.550154811464317 29.30653747891331 21.43282983984322 34-35 21.920529346222757 27.61231366507123 29.28525027889014 21.181906709815866 36-37 21.945191927692935 27.502121855332245 29.164424890632993 21.38826132634182 38-39 21.783895718427413 27.69782716182129 29.19557820757508 21.322698912176218 40-41 21.91213849123057 27.541083474267893 29.250664271037408 21.29611376346413 42-43 21.72446646431331 27.574219532442058 29.131688216821143 21.569625786423487 44-45 21.808644884023547 27.736973257078855 28.96503452027417 21.48934733862343 46-47 21.804617558126083 27.68129595676479 28.768617254400535 21.745469230708593 48-49 21.77641315683126 27.76237035655577 28.76974440330159 21.691472083311382 50-51 21.881156472155162 27.900747764602645 28.574466261656706 21.64362950158549 52-53 21.85895452285095 28.000549493009157 28.436532715281704 21.703963268858185 54-55 21.790814284064638 27.96781944784485 28.56123714057305 21.680129127517468 56-57 21.879690986185306 28.02079719807928 28.330283964230585 21.76922785150483 58-59 21.82048411812679 27.906276854232658 28.527066708482497 21.74617231915806 60-61 21.991968620458767 28.075298663642158 28.42638349008596 21.50634922581312 62-63 21.79169717284074 28.034624827625798 28.533477418154174 21.640200581379286 64-65 21.727540840577316 27.857270624933676 28.517133756996248 21.898054777492753 66-67 21.751086373196273 27.92918521083843 28.337666249040293 21.982062166925 68-69 21.70915715340769 27.959026186002085 28.40764724324308 21.92416941734715 70-71 21.731271115001434 27.79528527754841 28.397904993003888 22.075538614446273 72-73 21.691709379216427 28.010923147594546 28.20701885874115 22.090348614447873 74-75 21.73279363682728 28.034152332662533 28.127341606194474 22.10571242431572 76-77 21.58227456659744 27.93117624334547 28.270483297803434 22.216065892253656 78-79 21.60505854927167 27.817695489886944 28.27292449906348 22.304321461777903 80-81 21.595946230010078 28.08082611531227 27.98695366273022 22.336273991947436 82-83 21.68947016442708 28.05228784593952 28.030591085124414 22.227650904508984 84-85 21.61547949419152 27.999409735125376 28.01643038664708 22.36868038403603 86-87 21.491859275921414 27.991385994856206 28.15795586245543 22.35879886676695 88-89 21.520602092688527 27.832514838034967 28.143958588270813 22.5029244810057 90-91 21.646812490452184 27.925489206951344 28.007936504192816 22.419761798403655 92-93 21.58005669800344 27.863365427157422 27.9885822230564 22.567995651782738 94-95 21.402331729014666 27.762290533836907 28.077151523700607 22.75822621344782 96-97 21.431306795381808 27.72629119734124 28.093993229786644 22.748408777490305 98-99 21.364976105597076 27.63993026211542 27.99116673227447 23.003926900013035 100 20.682141312155423 28.099742463589795 28.125738682076623 23.09237754217816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2181.0 1 1566.0 2 1448.0 3 2038.0 4 1695.5 5 1468.5 6 2328.0 7 4668.5 8 7540.0 9 9015.5 10 7945.0 11 6425.0 12 6510.5 13 7086.0 14 7647.0 15 8198.0 16 8807.0 17 9434.0 18 10201.0 19 11298.5 20 12870.5 21 15191.5 22 18427.0 23 22945.5 24 29074.5 25 36928.5 26 46398.5 27 57740.5 28 70290.0 29 84336.5 30 100325.0 31 116017.5 32 132430.5 33 150853.0 34 171045.5 35 190164.0 36 212894.0 37 232993.0 38 243623.5 39 252849.0 40 256067.5 41 256331.5 42 259272.0 43 263974.0 44 269361.5 45 275765.0 46 278273.0 47 275318.0 48 275386.0 49 275408.0 50 271321.5 51 260324.5 52 245256.5 53 232672.0 54 218270.5 55 199380.0 56 178509.0 57 157278.5 58 137084.0 59 116518.5 60 95384.0 61 76606.5 62 60883.0 63 46493.0 64 33676.0 65 24437.5 66 17903.0 67 13287.5 68 9972.5 69 7290.5 70 5434.0 71 4094.5 72 3058.0 73 2258.5 74 1633.0 75 1172.0 76 811.5 77 537.5 78 345.0 79 233.5 80 167.0 81 105.5 82 68.5 83 57.0 84 53.5 85 34.5 86 18.5 87 12.5 88 14.0 89 8.5 90 5.0 91 6.0 92 4.0 93 1.5 94 0.5 95 1.0 96 1.5 97 3.5 98 3.5 99 3.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033685163639156444 2 0.06960702214395287 3 8.219179927954172E-4 4 7.410736000614418E-4 5 0.0016842581819578222 6 7.814957964284294E-4 7 0.0 8 8.623401891624049E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.208625018779479E-4 18-19 0.0010846622691808375 20-21 0.003220301643903356 22-23 6.737032727831288E-6 24-25 6.737032727831288E-6 26-27 1.4821472001228834E-4 28-29 9.027623855293927E-4 30-31 0.001495621265578546 32-33 0.002944083302062273 34-35 0.0038266345894081718 36-37 0.0012665621528322821 38-39 0.0014080398401167394 40-41 0.005012352349506479 42-43 0.006582080975091168 44-45 0.0050393004804178045 46-47 0.006622503171458156 48-49 0.008596453760712724 50-51 0.008259602124321159 52-53 0.0037525272294020276 54-55 0.0022097467347286623 56-57 0.0024859650765697455 58-59 0.003132720218441549 60-61 0.00706041029876719 62-63 0.013959131812066431 64-65 0.003739053163946365 66-67 0.010206604582664402 68-69 0.012470247579215714 70-71 0.005403100247720694 72-73 0.007329891607880442 74-75 0.0028295537456891414 76-77 0.0029103981384231165 78-79 0.007612846982449356 80-81 0.005726477818656595 82-83 0.01620256371043425 84-85 0.017078377965052315 86-87 0.022306315361849398 88-89 0.02081743112899868 90-91 0.02389625508561758 92-93 0.03412307076646548 94-95 0.03820571259953124 96-97 0.022400633820039033 98-99 0.01762407761600665 100 0.01825735869242279 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7421665.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.087627904996 #Duplication Level Percentage of deduplicated Percentage of total 1 73.74906302620504 45.05155320486912 2 16.37834397676566 20.01028365105386 3 5.107892292064504 9.360870711493007 4 1.9952593277076724 4.875426375399152 5 0.9522399004593818 2.9085038357776574 6 0.5038904876815744 1.846888476981539 7 0.31570070920597826 1.34997852173227 8 0.1926439965692747 0.9414531824444143 9 0.14496774317991143 0.7970161998241305 >10 0.6022020168496229 6.177406427552177 >50 0.03343187072676675 1.4156726712441163 >100 0.021950449069306786 2.7034848318706146 >500 0.0015585395359098532 0.6840928487330059 >1k 7.639856958930498E-4 0.730125304305263 >5k 0.0 0.0 >10k+ 9.167828348559674E-5 1.1472437567197786 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24024 0.3237009485068378 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22487 0.3029913099014844 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14944 0.20135643416942156 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3474065455662576E-5 0.0 4 0.0 0.0 0.0 1.3474065455662576E-5 0.0 5 0.0 0.0 0.0 2.6948130911325153E-5 0.0 6 0.0 0.0 0.0 2.6948130911325153E-5 0.0 7 0.0 0.0 0.0 2.6948130911325153E-5 0.0 8 0.0 0.0 0.0 2.6948130911325153E-5 1.3474065455662576E-5 9 0.0 0.0 0.0 8.084439273397547E-5 1.3474065455662576E-5 10-11 0.0 0.0 0.0 8.084439273397547E-5 1.3474065455662576E-5 12-13 0.0 0.0 0.0 1.0779252364530061E-4 5.3896261822650305E-5 14-15 0.0 0.0 0.0 1.2126658910096319E-4 6.737032727831289E-5 16-17 0.0 0.0 0.0 1.2126658910096319E-4 6.737032727831289E-5 18-19 0.0 0.0 0.0 1.2126658910096319E-4 7.410736000614419E-5 20-21 0.0 0.0 0.0 1.3474065455662575E-4 8.084439273397547E-5 22-23 0.0 0.0 0.0 1.8189988365144478E-4 8.084439273397547E-5 24-25 0.0 0.0 0.0 3.099035054802393E-4 8.084439273397547E-5 26-27 0.0 0.0 0.0 4.513811927646963E-4 8.084439273397547E-5 28-29 0.0 0.0 0.0 0.0011116104000921626 8.084439273397547E-5 30-31 0.0 0.0 0.0 0.0045340230258304574 8.084439273397547E-5 32-33 0.0 0.0 0.0 0.01270604372468981 8.084439273397547E-5 34-35 0.0 0.0 0.0 0.025068498780260226 8.084439273397547E-5 36-37 0.0 0.0 0.0 0.045724241123790955 8.084439273397547E-5 38-39 0.0 1.3474065455662576E-5 0.0 0.07911297532292283 8.084439273397547E-5 40-41 0.0 1.3474065455662576E-5 0.0 0.11593085918052082 8.758142546180675E-5 42-43 0.0 1.3474065455662576E-5 0.0 0.1511520662816228 9.431845818963805E-5 44-45 0.0 1.3474065455662576E-5 0.0 0.19745569222000725 1.0779252364530061E-4 46-47 0.0 1.3474065455662576E-5 0.0 0.25518532566479357 1.0779252364530061E-4 48-49 0.0 1.3474065455662576E-5 0.0 0.3174422451026825 1.2126658910096319E-4 50-51 0.0 1.3474065455662576E-5 0.0 0.3826769330062728 1.2126658910096319E-4 52-53 0.0 1.3474065455662576E-5 0.0 0.4483832132008114 1.4821472001228834E-4 54-55 0.0 1.3474065455662576E-5 0.0 0.5259736191272444 1.751628509236135E-4 56-57 0.0 1.3474065455662576E-5 0.0 0.6175231568657438 1.751628509236135E-4 58-59 0.0 1.3474065455662576E-5 0.0 0.7241434368164017 1.751628509236135E-4 60-61 0.0 1.3474065455662576E-5 0.0 0.8383967748476926 1.751628509236135E-4 62-63 0.0 1.3474065455662576E-5 0.0 0.9547453300573389 1.886369163792761E-4 64-65 0.0 1.3474065455662576E-5 0.0 1.0754796935728033 1.886369163792761E-4 66-67 1.3474065455662576E-5 1.3474065455662576E-5 0.0 1.220292212057537 1.886369163792761E-4 68-69 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 1.3888527709078757 1.886369163792761E-4 70-71 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 1.5737236859922943 1.886369163792761E-4 72-73 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 1.7632701017898276 2.290591127462638E-4 74-75 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 1.9534228505328657 2.290591127462638E-4 76-77 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 2.166852047350561 2.290591127462638E-4 78-79 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 2.4175639832840745 2.290591127462638E-4 80-81 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 2.688662180252005 2.290591127462638E-4 82-83 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 2.9702634112426254 2.290591127462638E-4 84-85 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 3.251393049942297 2.290591127462638E-4 86-87 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 3.5467782498940603 2.290591127462638E-4 88 1.3474065455662576E-5 1.3474065455662576E-5 1.3474065455662576E-5 3.7901468201542374 2.290591127462638E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12495 0.0 48.968258 1 GTATCAA 20905 0.0 35.837627 1 TCAACGC 25580 0.0 28.750858 4 ATCAACG 25775 0.0 28.442183 3 CAACGCA 26075 0.0 28.222893 5 AACGCAG 27010 0.0 27.210192 6 TATCAAC 27100 0.0 27.158575 2 TACCTGG 5785 0.0 25.36373 2 GTACATG 23635 0.0 24.872992 1 GTACCTG 6270 0.0 24.30251 1 ACGCAGA 30740 0.0 23.847033 7 TACATGG 24140 0.0 23.748072 2 CGCAGAG 31295 0.0 23.288977 8 ACATGGG 23805 0.0 23.136435 3 CATGGGG 12750 0.0 22.261978 4 TATAACG 765 0.0 20.901594 2 GCAGAGT 34890 0.0 20.40433 9 GAGTACT 21980 0.0 20.086178 12-13 ACCTGGG 7240 0.0 19.667133 3 TAACGCA 755 0.0 19.29531 4 >>END_MODULE