FastQCFastQC Report
Mon 6 Jun 2016
ERR1211508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10802330
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360760.3339649871833206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317290.2937236688751408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201430.18646903029253875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186300.046.9701161
TACCTGG79550.040.673792
GTATCAA308850.037.10081
ACCTGGG91700.034.4358023
GTACCTG101050.032.9978561
GTACATG410550.030.2161051
TCAACGC382700.029.4313244
ATCAACG384750.029.2874013
CAACGCA386450.029.0949345
TATCAAC397700.028.3924542
TACATGG434300.028.197952
AACGCAG407050.027.7549766
ATGGGGG163100.027.7421675
ACATGGG438050.026.9574643
TGGGGGG162150.026.5080436
CATGGGG275000.024.981294
CGCAGAG458400.023.9609268
ACGCAGA471550.023.809917
TATAACG6250.023.3264182
GCAGAGT492350.021.5282659
Produced by FastQC (version 0.11.5)