FastQCFastQC Report
Mon 6 Jun 2016
ERR1211508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10802330
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385560.3569229971682035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342740.31728340089591783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236650.21907310737590868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205200.043.3330761
TACCTGG78300.038.4944042
GTATCAA326050.035.0018421
ACCTGGG90450.032.7301333
GTACCTG98400.031.3483071
GTACATG416950.028.0589711
TCAACGC409600.027.613914
ATCAACG411000.027.4172023
ATGGGGG149650.027.2864325
TGGGGGG139050.027.2034876
CAACGCA415650.027.2001675
TATCAAC423400.026.753912
TACATGG440600.026.2858052
AACGCAG436150.026.0615166
ACATGGG444250.025.1007143
CATGGGG276700.023.5208174
TATAACG7600.023.5110132
CGCAGAG492150.022.5612898
ACGCAGA510300.022.1363777
CCTGGGG80900.021.0848184
Produced by FastQC (version 0.11.5)