##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10802330 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18070545891488 33.0 31.0 34.0 30.0 34.0 2 32.393791154315785 34.0 31.0 34.0 31.0 34.0 3 32.398700280402466 34.0 31.0 34.0 30.0 34.0 4 36.02011769683022 37.0 35.0 37.0 35.0 37.0 5 35.94906200791866 37.0 35.0 37.0 35.0 37.0 6 35.96929143990232 37.0 35.0 37.0 35.0 37.0 7 35.89976079234758 37.0 35.0 37.0 35.0 37.0 8 35.88816708987783 37.0 35.0 37.0 35.0 37.0 9 37.543119863955276 39.0 37.0 39.0 35.0 39.0 10-11 37.50729324136552 39.0 37.0 39.0 35.0 39.0 12-13 37.4341491141263 39.0 37.0 39.0 34.0 39.0 14-15 38.68785636987576 40.0 38.0 41.0 34.0 41.0 16-17 38.61764026834951 40.0 38.0 41.0 34.0 41.0 18-19 38.612628756944105 40.0 38.0 41.0 34.0 41.0 20-21 38.558784215997846 40.0 38.0 41.0 34.0 41.0 22-23 38.47545159238794 40.0 38.0 41.0 34.0 41.0 24-25 38.38066500467954 40.0 38.0 41.0 34.0 41.0 26-27 38.220021051013994 40.0 38.0 41.0 34.0 41.0 28-29 38.260703246429244 40.0 38.0 41.0 34.0 41.0 30-31 38.1869506393528 40.0 38.0 41.0 34.0 41.0 32-33 38.01934740005166 40.0 37.0 41.0 33.0 41.0 34-35 37.9262267492291 40.0 37.0 41.0 33.0 41.0 36-37 37.89941896794488 40.0 37.0 41.0 33.0 41.0 38-39 37.830544613986056 40.0 37.0 41.0 33.0 41.0 40-41 37.68758527095543 40.0 37.0 41.0 33.0 41.0 42-43 37.57271315540258 40.0 37.0 41.0 32.0 41.0 44-45 37.41980933742998 40.0 37.0 41.0 31.5 41.0 46-47 37.20028452195035 40.0 36.0 41.0 31.0 41.0 48-49 37.02308506590708 40.0 36.0 41.0 31.0 41.0 50-51 36.861433366690335 39.0 36.0 41.0 30.0 41.0 52-53 36.69618369370312 39.0 36.0 41.0 30.0 41.0 54-55 36.53342751054633 39.0 35.0 40.5 29.5 41.0 56-57 36.375656409311695 39.0 35.0 40.0 29.0 41.0 58-59 36.164932195183816 39.0 35.0 40.0 28.0 41.0 60-61 35.88835265169644 38.0 35.0 40.0 28.0 41.0 62-63 35.5937354718843 38.0 34.0 40.0 28.0 41.0 64-65 35.30915131272605 38.0 34.0 40.0 27.0 41.0 66-67 34.978911401521714 37.0 34.0 40.0 26.0 41.0 68-69 34.601066529165465 37.0 33.5 39.0 26.0 41.0 70-71 34.2137764260118 36.0 33.0 39.0 26.0 40.0 72-73 33.781237103476755 36.0 32.5 39.0 26.0 40.0 74-75 33.35567775655807 35.0 32.0 38.0 25.0 39.5 76-77 31.748261949042476 34.0 30.0 36.0 23.0 38.5 78-79 32.55060584151752 35.0 31.5 36.5 25.0 39.0 80-81 32.49023044102522 35.0 32.0 36.0 25.0 38.5 82-83 32.177302952233454 35.0 31.0 36.0 24.0 37.0 84-85 31.851224782060907 34.0 31.0 35.5 23.5 37.0 86-87 31.54190026596114 34.0 31.0 35.0 23.0 36.5 88-89 31.26082016564945 34.0 31.0 35.0 21.5 36.0 90-91 30.966889828398134 34.0 30.0 35.0 20.0 36.0 92-93 30.75662861623372 34.0 30.0 35.0 20.0 35.0 94-95 30.53661200870553 34.0 30.0 35.0 20.0 35.0 96-97 30.33370110892743 34.0 29.0 35.0 19.0 35.0 98-99 30.173307425342497 34.0 29.0 35.0 19.0 35.0 100 28.348069814567783 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30502896718326866 1101 2 0.22532722635855862 1101 3 0.2928860051890467 1101 4 0.16162100028965654 1101 5 0.1791599594402058 1101 6 0.18098036047673105 1101 7 0.22308516763492747 1101 8 0.20121493417311598 1101 9 0.21702466118513541 1101 10-11 0.23199210523308267 1101 12-13 0.24734676586951565 1101 14-15 0.3108293005917915 1101 16-17 0.3211743726145997 1101 18-19 0.3450537138610059 1101 20-21 0.32319974571134935 1101 22-23 0.26969380419876643 1101 24-25 0.2914689947613809 1101 26-27 0.2710890484393005 1101 28-29 0.35395789476059036 1101 30-31 0.3667066945720876 1101 32-33 0.4164154857940119 1101 34-35 0.4046983384018077 1101 36-37 0.4392303683174461 1101 38-39 0.42070938521897006 1101 40-41 0.4052092238994405 1101 42-43 0.40622194845850856 1101 44-45 0.35975431800692803 1101 46-47 0.4254006153172867 1101 48-49 0.4144877104520077 1101 50-51 0.36745305606259393 1101 52-53 0.37867039334092567 1101 54-55 0.3714307298568116 1101 56-57 0.35018654387850745 1101 58-59 0.39779181253584284 1101 60-61 0.388867355930536 1101 62-63 0.3880610241624751 1101 64-65 0.3773547621295066 1101 66-67 0.2879249333195375 1101 68-69 0.2736048271725693 1101 70-71 0.3418409271936511 1101 72-73 0.19504123854768096 1101 74-75 0.23319540436582287 1101 76-77 0.2680532616901452 1101 78-79 0.34046856631269407 1101 80-81 0.31723059968020806 1101 82-83 0.27466295300864374 1101 84-85 0.27623724799212823 1101 86-87 0.24966561872447102 1101 88-89 0.24705755385713601 1101 90-91 0.218279230221178 1101 92-93 0.13972453807277319 1101 94-95 0.16654365550307304 1101 96-97 0.09584468688885295 1101 98-99 0.017645927091571423 1101 100 0.09062726032870927 1102 1 0.3216137058733324 1102 2 0.24963395487899476 1102 3 0.31970154629598824 1102 4 0.21271778327273694 1102 5 0.18374141545049127 1102 6 0.14392243211459288 1102 7 0.16997505560027548 1102 8 0.17803019724394176 1102 9 0.22873253048776832 1102 10-11 0.22681356665579955 1102 12-13 0.2725537521757744 1102 14-15 0.3273467892954116 1102 16-17 0.33163455483020243 1102 18-19 0.3253078782628407 1102 20-21 0.316124016086647 1102 22-23 0.27057882792220056 1102 24-25 0.30826689447653166 1102 26-27 0.2844435866607 1102 28-29 0.3433237661976065 1102 30-31 0.36033665537362936 1102 32-33 0.4835140004787206 1102 34-35 0.4622613726655871 1102 36-37 0.4617653116652676 1102 38-39 0.4593560003840764 1102 40-41 0.4417277621894158 1102 42-43 0.370014810622564 1102 44-45 0.4355727404948624 1102 46-47 0.5153507449052839 1102 48-49 0.4924283015475268 1102 50-51 0.460151340996795 1102 52-53 0.46130510299938976 1102 54-55 0.45954350408731415 1102 56-57 0.45372059148662913 1102 58-59 0.5352557663114084 1102 60-61 0.5085292362053977 1102 62-63 0.5010369466979085 1102 64-65 0.4759631102566786 1102 66-67 0.4810802169557675 1102 68-69 0.4771174775843221 1102 70-71 0.4981568883668288 1102 72-73 0.5226776697131257 1102 74-75 0.4371307855278559 1102 76-77 0.44879469597653454 1102 78-79 0.4776813607934898 1102 80-81 0.47586648265110654 1102 82-83 0.4461074386951154 1102 84-85 0.43706440313118833 1102 86-87 0.41714484595087953 1102 88-89 0.3279886551164992 1102 90-91 0.28156135161823315 1102 92-93 0.20282211299539554 1102 94-95 0.3024184590821406 1102 96-97 0.27911781381744305 1102 98-99 0.25893169585912545 1102 100 0.3451155742389034 1103 1 0.2756002844483092 1103 2 0.23742417117770032 1103 3 0.31700304506165367 1103 4 0.1573678795866229 1103 5 0.1583537970133051 1103 6 0.18430558997204827 1103 7 0.19157442370229205 1103 8 0.2161642513529074 1103 9 0.2576959712858553 1103 10-11 0.2489066002064888 1103 12-13 0.2714218708471563 1103 14-15 0.3719150850153454 1103 16-17 0.4086763816090411 1103 18-19 0.35915165809759486 1103 20-21 0.3131790413197706 1103 22-23 0.3186096355507928 1103 24-25 0.32232882644381533 1103 26-27 0.30985095093920023 1103 28-29 0.41847112803283437 1103 30-31 0.42805277564549726 1103 32-33 0.481398836441457 1103 34-35 0.46959947676504754 1103 36-37 0.48409993568154164 1103 38-39 0.41512287915641366 1103 40-41 0.4491615763427248 1103 42-43 0.46891627394278856 1103 44-45 0.4264135921980383 1103 46-47 0.5168069473089716 1103 48-49 0.4963744215343695 1103 50-51 0.452451841403402 1103 52-53 0.41248578199072483 1103 54-55 0.5187919202844569 1103 56-57 0.5371773703057983 1103 58-59 0.6071521607945627 1103 60-61 0.5738963585757872 1103 62-63 0.5601206977227662 1103 64-65 0.5128738638690464 1103 66-67 0.5366825645246678 1103 68-69 0.5107346559303849 1103 70-71 0.5311399961005279 1103 72-73 0.5266894053053903 1103 74-75 0.4849273418531297 1103 76-77 0.5199513495018273 1103 78-79 0.47981814429417824 1103 80-81 0.4603476784359515 1103 82-83 0.45684671056789483 1103 84-85 0.4062184963373028 1103 86-87 0.4135522095304154 1103 88-89 0.3878539880904093 1103 90-91 0.4782932168395284 1103 92-93 0.43033483246255955 1103 94-95 0.48674826620041145 1103 96-97 0.4548726252984352 1103 98-99 0.44200987486774324 1103 100 0.5068534692227153 1104 1 0.2607623644419945 1104 2 0.22002771680094924 1104 3 0.2742370904150988 1104 4 0.09812600144791617 1104 5 0.14561753023984636 1104 6 0.16432315075321924 1104 7 0.1891286994892809 1104 8 0.1785033323637606 1104 9 0.2224168507538451 1104 10-11 0.2351749718106717 1104 12-13 0.28998910664106603 1104 14-15 0.3991899488440822 1104 16-17 0.34574128693311934 1104 18-19 0.35724403833551577 1104 20-21 0.26102916174461654 1104 22-23 0.3113393969385143 1104 24-25 0.3518561330825136 1104 26-27 0.377511287075869 1104 28-29 0.4201591440217953 1104 30-31 0.39083764479511274 1104 32-33 0.4102530424741673 1104 34-35 0.4975619185033011 1104 36-37 0.4079760174839464 1104 38-39 0.500825507821105 1104 40-41 0.536894411807225 1104 42-43 0.5653351154952801 1104 44-45 0.5366546863061572 1104 46-47 0.5160979354695456 1104 48-49 0.5349842619644036 1104 50-51 0.524467401684447 1104 52-53 0.49316497434520556 1104 54-55 0.5342350667853637 1104 56-57 0.6023684801133271 1104 58-59 0.6378154657185391 1104 60-61 0.5963799870016899 1104 62-63 0.5900941685340797 1104 64-65 0.6998456068047787 1104 66-67 0.6425233104254247 1104 68-69 0.6624774788541643 1104 70-71 0.597271190553279 1104 72-73 0.6403928311618543 1104 74-75 0.6376461606555779 1104 76-77 0.5797711916804182 1104 78-79 0.6602777328755352 1104 80-81 0.724665449608068 1104 82-83 0.5934069784544178 1104 84-85 0.5590482451278831 1104 86-87 0.6804300192084014 1104 88-89 0.6433063850242284 1104 90-91 0.5613739494286918 1104 92-93 0.6132405212907805 1104 94-95 0.5953154455892467 1104 96-97 0.5664221292157734 1104 98-99 0.7039138847047859 1104 100 0.8276092220707021 1105 1 0.25103191203767494 1105 2 0.19283227287689186 1105 3 0.25244959264896494 1105 4 0.1261423617798414 1105 5 0.10885958694801445 1105 6 0.13524657212617797 1105 7 0.13344729436200709 1105 8 0.15335883070985545 1105 9 0.1686804773390449 1105 10-11 0.20475507235994428 1105 12-13 0.2288904489840462 1105 14-15 0.2890436769564104 1105 16-17 0.29030968708013205 1105 18-19 0.26610783486315626 1105 20-21 0.23924324875919467 1105 22-23 0.28407575686581055 1105 24-25 0.2705465815754522 1105 26-27 0.31442266748729963 1105 28-29 0.32669042995332376 1105 30-31 0.3668401366098877 1105 32-33 0.42706625077841665 1105 34-35 0.4248679598896743 1105 36-37 0.4098606083576328 1105 38-39 0.3775926900472797 1105 40-41 0.4069951465385486 1105 42-43 0.40167535105439356 1105 44-45 0.4960034425904851 1105 46-47 0.5281032900052267 1105 48-49 0.5161616528758515 1105 50-51 0.5745764143660708 1105 52-53 0.5452678771914066 1105 54-55 0.5762896001011626 1105 56-57 0.5593135792632751 1105 58-59 0.5831660057241379 1105 60-61 0.6517830608018969 1105 62-63 0.6095703816499949 1105 64-65 0.6295025523591349 1105 66-67 0.5657887510787631 1105 68-69 0.5891670405442895 1105 70-71 0.6324527153820725 1105 72-73 0.6561998048713562 1105 74-75 0.6484256704525606 1105 76-77 0.6028147630343277 1105 78-79 0.7170739637949524 1105 80-81 0.7329764611365093 1105 82-83 0.6778916549736991 1105 84-85 0.695894649530203 1105 86-87 0.6299611938680982 1105 88-89 0.6387149381194384 1105 90-91 0.7263648622286603 1105 92-93 0.6721707605926674 1105 94-95 0.7112274025911098 1105 96-97 0.7316016646389905 1105 98-99 0.773974460418529 1105 100 0.8192667003811209 1106 1 0.23788811363318274 1106 2 0.1684666983031775 1106 3 0.23048317565768173 1106 4 0.14439139470504614 1106 5 0.1318562600483162 1106 6 0.10303888046400544 1106 7 0.11253823052289391 1106 8 0.14269472305798558 1106 9 0.1754693559112681 1106 10-11 0.15064681141623026 1106 12-13 0.1527461876830074 1106 14-15 0.2440582406019729 1106 16-17 0.25997606319575794 1106 18-19 0.25216433531117133 1106 20-21 0.24236417303635704 1106 22-23 0.20571363912767282 1106 24-25 0.23262023626545414 1106 26-27 0.24087677170128785 1106 28-29 0.2609303727815373 1106 30-31 0.28492442167836174 1106 32-33 0.3282675349779467 1106 34-35 0.32403314898697744 1106 36-37 0.3597434765837093 1106 38-39 0.3505206595556558 1106 40-41 0.3637444758799546 1106 42-43 0.3063182439682848 1106 44-45 0.3752112398255534 1106 46-47 0.4396936509291791 1106 48-49 0.36795901788466523 1106 50-51 0.4030964177227432 1106 52-53 0.4609746065160323 1106 54-55 0.34187983379624143 1106 56-57 0.3888590473437148 1106 58-59 0.42806692962231807 1106 60-61 0.5181960321916463 1106 62-63 0.47097018349779063 1106 64-65 0.5237221791724807 1106 66-67 0.5547474098157323 1106 68-69 0.4516214614769538 1106 70-71 0.5518090049549897 1106 72-73 0.5443962273037002 1106 74-75 0.5178399303730217 1106 76-77 0.5254431220796256 1106 78-79 0.560951358472451 1106 80-81 0.5294572563440383 1106 82-83 0.49200427418309545 1106 84-85 0.5569433267559702 1106 86-87 0.4548523590409488 1106 88-89 0.548403471008104 1106 90-91 0.5487062254183712 1106 92-93 0.60524938803675 1106 94-95 0.6844834195693963 1106 96-97 0.683863415037596 1106 98-99 0.6561298528925334 1106 100 0.7100978297674736 1107 1 0.1908136633233397 1107 2 0.1714553269652228 1107 3 0.2406308533628021 1107 4 0.1565463866986576 1107 5 0.14774743832705184 1107 6 0.16047910595752057 1107 7 0.18160583825564203 1107 8 0.18102025253194398 1107 9 0.20438769515572375 1107 10-11 0.16616860210783102 1107 12-13 0.21133571259671413 1107 14-15 0.25938735596634643 1107 16-17 0.24370661821890138 1107 18-19 0.23153387976720552 1107 20-21 0.24906388343649155 1107 22-23 0.29410795064168127 1107 24-25 0.30821037263645223 1107 26-27 0.32637324795083344 1107 28-29 0.3415654874422174 1107 30-31 0.24284978080594755 1107 32-33 0.2681822319917515 1107 34-35 0.3228674860418934 1107 36-37 0.40028407497226937 1107 38-39 0.35098530080919943 1107 40-41 0.29614424134990713 1107 42-43 0.3515972358536388 1107 44-45 0.37221056981861267 1107 46-47 0.37492788270518673 1107 48-49 0.4049686713977252 1107 50-51 0.34747698995175824 1107 52-53 0.393417013812865 1107 54-55 0.4239128353665791 1107 56-57 0.5077520417665795 1107 58-59 0.56368333600998 1107 60-61 0.517688528169117 1107 62-63 0.517216542786116 1107 64-65 0.5665460209249105 1107 66-67 0.4907171164432711 1107 68-69 0.44828651986919255 1107 70-71 0.5130575681408445 1107 72-73 0.5250889802368448 1107 74-75 0.6138038427937786 1107 76-77 0.4927080079160753 1107 78-79 0.5625393798751119 1107 80-81 0.544451382045736 1107 82-83 0.5448917804039937 1107 84-85 0.4657826681496431 1107 86-87 0.4071948286986924 1107 88-89 0.4118208709724236 1107 90-91 0.3431922422685183 1107 92-93 0.4596636180341669 1107 94-95 0.5892683904243725 1107 96-97 0.6624037913793366 1107 98-99 0.6789585028975864 1107 100 0.7877990265556392 1108 1 0.2151074726463449 1108 2 0.16604971843004535 1108 3 0.21985748986271858 1108 4 0.14770374109768625 1108 5 0.11327061230274182 1108 6 0.10719250279059622 1108 7 0.12918613842455784 1108 8 0.12586899563893184 1108 9 0.11514227331194604 1108 10-11 0.1738222638493383 1108 12-13 0.1846724740322756 1108 14-15 0.21719177400964895 1108 16-17 0.2654373000088768 1108 18-19 0.25928423673311585 1108 20-21 0.2805711150381285 1108 22-23 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-0.25450561797539706 2316 66-67 0.10738488428909676 2316 68-69 -0.03603354681617077 2316 70-71 -0.24979092625275 2316 72-73 -0.21299483358192361 2316 74-75 -0.2725658768280752 2316 76-77 -0.2953797624650214 2316 78-79 -0.21550298607233032 2316 80-81 -0.1449908386997123 2316 82-83 -0.023403267746985534 2316 84-85 -0.11577452722386994 2316 86-87 -0.10442480737440007 2316 88-89 -0.10877522547550811 2316 90-91 0.019174967840587698 2316 92-93 0.08142131124309415 2316 94-95 0.15479778230458408 2316 96-97 0.08896494331516891 2316 98-99 -0.0468609280488792 2316 100 -0.25121448337610275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 17.0 11 59.0 12 124.0 13 278.0 14 827.0 15 2250.0 16 5001.0 17 10291.0 18 18848.0 19 28690.0 20 41031.0 21 57244.0 22 75754.0 23 86820.0 24 93958.0 25 105029.0 26 123501.0 27 149012.0 28 181674.0 29 220064.0 30 266909.0 31 323437.0 32 396422.0 33 497905.0 34 659437.0 35 873973.0 36 1278311.0 37 2183700.0 38 2667770.0 39 453920.0 40 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.431790550076464 20.61557099162736 14.170987868788021 23.781650589508153 2 14.892035611387492 21.883846103962277 40.43248751655967 22.791630768090563 3 16.461977715000696 28.131173485805355 31.081162395517083 24.325686403676865 4 11.828071660506849 18.149494161781583 39.17434746110724 30.848086716604328 5 11.410484059176623 39.31369907030726 35.8139951681935 13.461821702322615 6 28.904850770620932 38.99379231925755 18.77952795631804 13.321828953803477 7 26.103775759488922 33.580283142618306 23.737730656256566 16.5782104416362 8 25.185360046360838 36.590534167976585 21.143246480815982 17.080859304846598 9 26.902985458043354 15.741077687968955 21.0505067934887 36.30543006049899 10-11 23.582190138608986 27.070192264076358 29.906580339611917 19.441037257702735 12-13 24.25261957374011 24.88235408472061 29.783569840950978 21.0814565005883 14-15 21.87770601342488 26.22117635732291 27.60888160239504 24.292236026857168 16-17 21.105353005081945 29.31897720101984 27.978785364302716 21.59688442959549 18-19 21.164564581188902 28.521476486892634 29.6665894910939 20.647369440824555 20-21 21.872685891290875 27.80940839820977 29.718370163891784 20.599535546607576 22-23 21.53236021915458 27.696132579551474 29.431920216838414 21.33958698445553 24-25 21.19551718893213 27.77077980128174 29.587475997074336 21.446227012711798 26-27 21.412165556704178 27.695238901061686 29.497509654641686 21.39508588759245 28-29 21.106936358928586 28.436320325473297 29.370122191345903 21.086621124252215 30-31 22.049673139930327 27.827203518994693 29.400071800348375 20.72305154072661 32-33 21.561947018784227 27.569948992129717 29.57868620237965 21.289417786706405 34-35 21.54099742475086 28.12908971527377 29.57106259635197 20.758850263623398 36-37 21.597700539714783 27.99587713117723 29.638007562727807 20.76841476638018 38-39 21.63044855299193 28.131296044863586 29.247206243820393 20.991049158324092 40-41 21.650173094901852 28.21364737428488 29.287345406179483 20.848834124633793 42-43 21.671265545808495 27.943067403783438 28.889543116285683 21.496123934122387 44-45 21.257485473425383 28.256163003141623 29.149369708903784 21.336981814529214 46-47 21.160721307672077 28.03687961660041 29.23900506560316 21.563394010124355 48-49 21.1765818485079 28.317654166846427 29.096392480147447 21.40937150449822 50-51 21.509994668288186 28.1392362792669 28.931855094090043 21.418913958354878 52-53 21.446926326741732 28.26314563325384 28.845515158924055 21.444412881080368 54-55 21.405003871014806 28.321432998487232 28.95431591535591 21.319247215142052 56-57 21.54852898721351 28.31493461273074 28.44640948033591 21.690126919719837 58-59 21.127912218576128 28.751399108671556 28.707772793037268 21.41291587971505 60-61 21.8329530325175 28.33309741011858 28.773192185519527 21.060757371844396 62-63 21.39811158563043 28.366999731361357 28.867691604322644 21.367197078685567 64-65 21.581063809341654 28.184926389788167 28.898962941512686 21.335046859357494 66-67 21.487417108118652 28.298280185425806 28.716683022112328 21.497619684343217 68-69 20.91328137665297 27.96496243237035 29.054226023873337 22.067530167103342 70-71 21.321467652433775 27.894135574453742 29.078787306489513 21.70560946662297 72-73 21.236015570953075 28.60644413785186 28.363834820507943 21.79370547068712 74-75 21.243475925153007 28.457966227675858 28.533785446699945 21.76477240047119 76-77 21.11817402750742 27.954133134554578 28.733477881188037 22.194214956749974 78-79 21.079163908374188 27.912933916380933 28.81925084706832 22.188651328176558 80-81 21.008798994569904 28.213662319668614 28.671829011338957 22.105709674422528 82-83 21.228227875531925 28.29632120589565 28.51216233563953 21.963288582932893 84-85 21.442755701907583 28.385429578464034 28.073321995834892 22.09849272379349 86-87 21.02788397626584 28.526034533820827 28.083237803691553 22.36284368622178 88-89 20.540237236605353 28.038104709014437 28.81121280113778 22.610445253242425 90-91 21.305798315390476 28.00160079202537 28.674816804316137 22.017784088268012 92-93 21.235589312795494 28.148153738753283 28.27761106027348 22.338645888177748 94-95 20.96299179846419 28.027935869262176 28.344573133544365 22.664499198729267 96-97 20.88296042575081 27.796545290931896 28.539602698688505 22.780891584628787 98-99 20.798113024114958 27.846157763405817 28.3808533982695 22.974875814209724 100 19.957727151055714 28.30235568172885 28.665798852311042 23.074118314904393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6526.0 1 4588.0 2 3774.0 3 4932.0 4 3901.0 5 3241.5 6 4624.5 7 8869.0 8 14255.0 9 16975.5 10 14465.5 11 11031.0 12 10838.0 13 11295.5 14 11907.5 15 12524.0 16 13162.5 17 14048.0 18 15448.5 19 16932.5 20 18538.0 21 20968.0 22 24294.0 23 29468.5 24 36029.0 25 43725.5 26 54911.5 27 69138.0 28 84650.0 29 103198.0 30 127082.5 31 153784.5 32 181606.5 33 210648.0 34 242267.0 35 275758.0 36 311696.0 37 347433.0 38 367552.5 39 376165.5 40 381182.5 41 387494.5 42 402992.0 43 428653.5 44 464900.5 45 501325.0 46 510125.0 47 488859.0 48 470412.0 49 465687.0 50 457570.0 51 429059.5 52 384370.5 53 334990.0 54 282241.5 55 235747.5 56 200006.0 57 165852.5 58 134378.0 59 104353.0 60 77413.5 61 58472.0 62 44933.0 63 33328.0 64 23173.0 65 16123.0 66 11317.5 67 8008.0 68 5772.5 69 4151.0 70 3039.0 71 2099.0 72 1444.0 73 1067.0 74 808.0 75 603.5 76 405.5 77 255.0 78 167.0 79 113.5 80 87.5 81 77.0 82 62.0 83 39.0 84 29.0 85 30.5 86 22.5 87 16.0 88 16.0 89 12.5 90 9.5 91 4.5 92 3.0 93 3.0 94 2.0 95 2.0 96 4.5 97 6.5 98 5.5 99 5.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033233570905536122 2 0.07433581458814904 3 7.961245397983583E-4 4 5.739502496220723E-4 5 0.001471904672417895 6 6.202365600754652E-4 7 0.0 8 0.0010182988299746443 9 9.257262090678585E-6 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.526369773928402E-4 18-19 0.0011201287129721088 20-21 0.0035640459049112554 22-23 4.6286310453392925E-6 24-25 9.257262090678585E-6 26-27 1.2034440717882161E-4 28-29 8.516681123424298E-4 30-31 0.001425618361964502 32-33 0.003420558342505737 34-35 0.004281483716938845 36-37 0.0013237884789670377 38-39 0.0014950478276445915 40-41 0.005521956837089776 42-43 0.007266950741182689 44-45 0.005322925702140186 46-47 0.006975346985326314 48-49 0.008775884461963299 50-51 0.008525938385514976 52-53 0.003938965019583738 54-55 0.002342087308941682 56-57 0.0024578030850751645 58-59 0.0033650147699616655 60-61 0.007341008837908118 62-63 0.01458481642386411 64-65 0.003999137223173148 66-67 0.010520878366056212 68-69 0.013043482285766126 70-71 0.005461784633500366 72-73 0.007475239138222957 74-75 0.0029576952379718083 76-77 0.003434444235641755 78-79 0.007503010924494994 80-81 0.005410869692001633 82-83 0.016602899559632043 84-85 0.01747308219615583 86-87 0.02271269253947991 88-89 0.02177770906832137 90-91 0.02477243335465589 92-93 0.03508965195471718 94-95 0.038598154287084366 96-97 0.023064468498925694 98-99 0.0183293789395436 100 0.018671897636898707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.080233E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.76240422117958 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03825028104036 32.97432816646015 2 17.99833184881436 17.19287202140004 3 6.261319628273207 8.971670371307724 4 2.5822296754376977 4.933339902007226 5 1.32918706102009 3.174258484700162 6 0.7313699491440473 2.095919228774492 7 0.44964036430353216 1.5033133383816624 8 0.2964578547026825 1.1327631912682596 9 0.21520699322885142 0.9250923061638954 >10 0.9285707527923275 7.844662478742019 >50 0.07842016344531418 2.618950989108944 >100 0.07591517818912981 7.496714090596241 >500 0.009803136637373967 3.268744610375858 >1k 0.005024065913889809 4.09884178930377 >5k 1.9113294001365396E-4 0.5101006904744773 >10k+ 8.191411714870885E-5 1.2584283409350792 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38556 0.3569229971682035 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34274 0.31728340089591783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23665 0.21907310737590868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.851452418135717E-5 7 0.0 0.0 0.0 0.0 1.851452418135717E-5 8 0.0 0.0 0.0 0.0 1.851452418135717E-5 9 0.0 0.0 0.0 9.257262090678585E-6 1.851452418135717E-5 10-11 0.0 0.0 0.0 1.3885893136017878E-5 1.851452418135717E-5 12-13 0.0 0.0 0.0 3.702904836271434E-5 1.851452418135717E-5 14-15 0.0 0.0 0.0 3.702904836271434E-5 2.3143155226696465E-5 16-17 0.0 0.0 0.0 3.702904836271434E-5 2.7771786272035757E-5 18-19 0.0 0.0 0.0 4.165767940805363E-5 4.628631045339292E-5 20-21 0.0 0.0 0.0 5.554357254407151E-5 4.628631045339292E-5 22-23 0.0 0.0 0.0 1.3885893136017876E-4 5.554357254407151E-5 24-25 0.0 0.0 0.0 3.3326143526442905E-4 7.405809672542868E-5 26-27 0.0 0.0 0.0 5.924647738034294E-4 7.405809672542868E-5 28-29 0.0 0.0 0.0 0.001786651583500967 7.405809672542868E-5 30-31 0.0 0.0 0.0 0.006378253580477545 7.405809672542868E-5 32-33 0.0 0.0 0.0 0.016778787539354936 7.405809672542868E-5 34-35 0.0 0.0 0.0 0.0323124733275136 8.331535881610726E-5 36-37 0.0 0.0 0.0 0.05724690876875636 8.331535881610726E-5 38-39 0.0 0.0 0.0 0.09871944293499643 8.331535881610726E-5 40-41 0.0 0.0 0.0 0.14717658134865347 8.331535881610726E-5 42-43 0.0 0.0 0.0 0.19366655156804136 8.331535881610726E-5 44-45 0.0 0.0 0.0 0.25086254539529895 8.331535881610726E-5 46-47 9.257262090678585E-6 0.0 0.0 0.32056047167601803 8.331535881610726E-5 48-49 1.851452418135717E-5 0.0 0.0 0.39671996689603073 8.331535881610726E-5 50-51 1.851452418135717E-5 0.0 0.0 0.4807944212035737 8.331535881610726E-5 52-53 1.851452418135717E-5 4.6286310453392925E-6 0.0 0.5701270003786221 9.257262090678585E-5 54-55 2.3143155226696465E-5 2.7771786272035757E-5 0.0 0.6690871321279761 1.0182988299746443E-4 56-57 2.7771786272035757E-5 2.7771786272035757E-5 0.0 0.783705922703713 1.0182988299746443E-4 58-59 2.7771786272035757E-5 2.7771786272035757E-5 0.0 0.9191257811972047 1.0182988299746443E-4 60-61 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.0601786836728744 1.0182988299746443E-4 62-63 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.2141269522408593 1.0182988299746443E-4 64-65 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.363978882333719 1.0182988299746443E-4 66-67 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.537756206299937 1.0182988299746443E-4 68-69 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.746026088816024 1.0182988299746443E-4 70-71 2.7771786272035757E-5 2.7771786272035757E-5 0.0 1.978225993836515 1.1108714508814303E-4 72-73 2.7771786272035757E-5 2.7771786272035757E-5 0.0 2.201844416898947 1.851452418135717E-4 74-75 2.7771786272035757E-5 2.7771786272035757E-5 0.0 2.436705784770508 1.851452418135717E-4 76-77 2.7771786272035757E-5 2.7771786272035757E-5 0.0 2.6937382953492444 1.851452418135717E-4 78-79 2.7771786272035757E-5 2.7771786272035757E-5 0.0 2.9894754187291075 1.851452418135717E-4 80-81 2.7771786272035757E-5 2.7771786272035757E-5 0.0 3.3064672158691693 1.944025039042503E-4 82-83 2.7771786272035757E-5 2.7771786272035757E-5 0.0 3.6327486755172265 2.0365976599492886E-4 84-85 2.7771786272035757E-5 2.7771786272035757E-5 0.0 3.9610852473494145 2.0365976599492886E-4 86-87 2.7771786272035757E-5 2.7771786272035757E-5 0.0 4.312453887263211 2.0365976599492886E-4 88 2.7771786272035757E-5 2.7771786272035757E-5 0.0 4.5932683041529 2.0365976599492886E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20520 0.0 43.333076 1 TACCTGG 7830 0.0 38.494404 2 GTATCAA 32605 0.0 35.001842 1 ACCTGGG 9045 0.0 32.730133 3 GTACCTG 9840 0.0 31.348307 1 GTACATG 41695 0.0 28.058971 1 TCAACGC 40960 0.0 27.61391 4 ATCAACG 41100 0.0 27.417202 3 ATGGGGG 14965 0.0 27.286432 5 TGGGGGG 13905 0.0 27.203487 6 CAACGCA 41565 0.0 27.200167 5 TATCAAC 42340 0.0 26.75391 2 TACATGG 44060 0.0 26.285805 2 AACGCAG 43615 0.0 26.061516 6 ACATGGG 44425 0.0 25.100714 3 CATGGGG 27670 0.0 23.520817 4 TATAACG 760 0.0 23.511013 2 CGCAGAG 49215 0.0 22.561289 8 ACGCAGA 51030 0.0 22.136377 7 CCTGGGG 8090 0.0 21.084818 4 >>END_MODULE