FastQCFastQC Report
Mon 6 Jun 2016
ERR1211507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8723198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259040.2969553138653966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225810.258861486349387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144930.16614319656621346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135100.047.5409131
GTATCAA228650.037.295441
TCAACGC272900.030.2876724
ATCAACG275150.030.0067023
CAACGCA276350.029.9073245
AACGCAG287700.028.868156
TATCAAC291850.028.4832922
GTACATG268900.027.387631
TACCTGG79350.027.138522
GTACCTG84850.026.30481
TACATGG274300.025.9860942
ACGCAGA322850.025.5080727
CGCAGAG327600.024.852138
ACATGGG275750.024.8120063
CATGGGG155600.022.3171314
ACCTGGG96350.021.9958533
GCAGAGT364100.021.4457729
GAGTACT220800.020.09846312-13
AGAGTAC327800.019.4848310-11
CAGAGTA351450.019.04924210-11
Produced by FastQC (version 0.11.5)