FastQCFastQC Report
Mon 6 Jun 2016
ERR1211507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8723198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279900.32086856219473636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247050.28321035473458245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170460.19540998610830568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142800.047.2867281
GTATCAA237000.037.1027681
TACCTGG75700.029.5667862
TCAACGC293100.029.3225754
CAACGCA295100.029.1396035
ATCAACG297800.028.8757383
AACGCAG308250.027.9878036
TATCAAC310950.027.9450232
GTACCTG85400.027.2558861
GTACATG278650.025.363721
ACGCAGA350900.024.4250647
TACATGG288400.024.0649072
CGCAGAG357050.023.8464328
ACATGGG282850.023.358013
ACCTGGG96350.022.7757053
CATGGGG158950.022.024194
GCAGAGT399700.020.7139789
GAGTACT243500.019.66445412-13
TATAACG9350.019.6130922
AGAGTAC365000.019.38770110-11
Produced by FastQC (version 0.11.5)