FastQCFastQC Report
Mon 6 Jun 2016
ERR1211506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499115
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228210.3043159092772947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199850.26649811344405305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129520.17271371355153242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134900.046.328861
GTATCAA216500.035.2411231
TACCTGG62200.032.810522
GTACCTG71400.029.4190921
TCAACGC257550.028.7707164
GTACATG273400.028.7163831
ATCAACG259750.028.546083
CAACGCA260400.028.454125
TACATGG277050.027.784612
AACGCAG270100.027.4621056
TATCAAC276150.027.0918662
CATGGGG167000.026.6730334
ACATGGG277500.026.601633
ACCTGGG78700.025.0169453
ACGCAGA301950.024.348547
CGCAGAG305950.023.9694278
ATGGGGG110450.023.9495375
GCAGAGT336300.021.0819639
GAGTACT186800.020.72659712-13
TGGGGGG115900.019.3740636
Produced by FastQC (version 0.11.5)