FastQCFastQC Report
Mon 6 Jun 2016
ERR1211506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499115
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244770.3263985150247729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218460.29131437509626135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152680.20359735782155627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131850.043.2975921
GTATCAA216500.035.8603361
TACCTGG62850.033.818132
GTACCTG72400.029.682661
TCAACGC264650.028.6762034
ATCAACG266900.028.3994373
CAACGCA268200.028.3842415
AACGCAG278250.027.4772596
TATCAAC279700.027.3702282
GTACATG273800.027.290761
CATGGGG160350.026.3165154
TACATGG282000.026.1298182
ACCTGGG80200.025.7811473
ACATGGG277900.025.2461513
ATGGGGG102250.025.0469135
ACGCAGA314800.024.1972737
CGCAGAG321850.023.4190358
TATAACG6050.023.317522
TGGGGGG97900.023.0878476
GCAGAGT361300.020.2895269
Produced by FastQC (version 0.11.5)