##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7499115 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12917230899913 33.0 31.0 34.0 30.0 34.0 2 32.342952735089405 34.0 31.0 34.0 30.0 34.0 3 32.3548046669507 34.0 31.0 34.0 30.0 34.0 4 35.99166408302846 37.0 35.0 37.0 35.0 37.0 5 35.91381463012635 37.0 35.0 37.0 35.0 37.0 6 35.931827555651566 37.0 35.0 37.0 35.0 37.0 7 35.855354531834756 37.0 35.0 37.0 35.0 37.0 8 35.84711982680623 37.0 35.0 37.0 35.0 37.0 9 37.49695290710971 39.0 37.0 39.0 35.0 39.0 10-11 37.458417626613276 39.0 37.0 39.0 34.5 39.0 12-13 37.37115279602993 39.0 37.0 39.0 34.0 39.0 14-15 38.61021840577188 40.0 38.0 41.0 34.0 41.0 16-17 38.53961480788067 40.0 38.0 41.0 34.0 41.0 18-19 38.520893865476125 40.0 38.0 41.0 34.0 41.0 20-21 38.46152579337695 40.0 38.0 41.0 34.0 41.0 22-23 38.361297699795244 40.0 38.0 41.0 34.0 41.0 24-25 38.25436241476494 40.0 38.0 41.0 33.5 41.0 26-27 38.0841878675017 40.0 37.0 41.0 33.0 41.0 28-29 38.12709286362457 40.0 38.0 41.0 33.0 41.0 30-31 38.05590292987906 40.0 37.5 41.0 33.0 41.0 32-33 37.8860190835852 40.0 37.0 41.0 33.0 41.0 34-35 37.78591773829312 40.0 37.0 41.0 33.0 41.0 36-37 37.74237113312704 40.0 37.0 41.0 33.0 41.0 38-39 37.66057861494323 40.0 37.0 41.0 32.5 41.0 40-41 37.5089749257079 40.0 37.0 41.0 32.0 41.0 42-43 37.36786167434424 40.0 36.0 41.0 31.0 41.0 44-45 37.198206455028355 40.0 36.0 41.0 31.0 41.0 46-47 36.95454083581863 39.0 36.0 41.0 30.5 41.0 48-49 36.75022239290903 39.0 36.0 41.0 30.0 41.0 50-51 36.56272053435639 39.0 35.0 41.0 30.0 41.0 52-53 36.37257963106313 39.0 35.0 40.0 29.0 41.0 54-55 36.18288258014446 39.0 35.0 40.0 28.5 41.0 56-57 35.98583572861598 38.5 35.0 40.0 28.0 41.0 58-59 35.73284514239347 38.0 34.0 40.0 28.0 41.0 60-61 35.425327255282795 38.0 34.0 40.0 27.0 41.0 62-63 35.096236955960805 37.5 34.0 40.0 26.5 41.0 64-65 34.777626426585 37.0 33.5 40.0 26.0 41.0 66-67 34.43843273506273 36.5 33.0 39.0 26.0 41.0 68-69 34.03315277869456 36.0 32.5 39.0 26.0 40.0 70-71 33.6434283245423 35.5 32.0 38.5 25.0 40.0 72-73 33.23052233496885 35.0 32.0 38.0 24.5 40.0 74-75 32.820153578122216 35.0 31.0 37.0 24.0 39.0 76-77 31.27204423455301 33.5 29.5 35.5 22.0 38.5 78-79 32.10406314611791 34.0 31.0 36.0 23.5 38.5 80-81 32.074320569827236 34.5 31.0 36.0 24.0 37.5 82-83 31.790982735962842 34.0 31.0 35.5 23.0 37.0 84-85 31.49831973506207 34.0 31.0 35.0 22.5 37.0 86-87 31.20874916573489 34.0 30.5 35.0 21.0 36.0 88-89 30.937671045183333 34.0 30.0 35.0 20.0 36.0 90-91 30.65737030302909 34.0 30.0 35.0 20.0 35.5 92-93 30.44767755928533 34.0 29.0 35.0 19.5 35.0 94-95 30.234879582457395 34.0 29.0 35.0 19.0 35.0 96-97 30.03625481140108 34.0 29.0 35.0 18.0 35.0 98-99 29.87934102890808 34.0 29.0 35.0 18.0 35.0 100 28.008589813598004 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2961217147706279 1101 2 0.2569856736249818 1101 3 0.31723790032267374 1101 4 0.16580006183865947 1101 5 0.14582074695763936 1101 6 0.19274430314580115 1101 7 0.1964644377632041 1101 8 0.18467893196920926 1101 9 0.2645404525457806 1101 10-11 0.24983061653002636 1101 12-13 0.22798511313379777 1101 14-15 0.293590928871005 1101 16-17 0.3253279737008441 1101 18-19 0.3768228657987862 1101 20-21 0.2717102172384145 1101 22-23 0.26334459774696484 1101 24-25 0.2933580524524544 1101 26-27 0.20607267030923992 1101 28-29 0.25917099066931115 1101 30-31 0.29724234506473834 1101 32-33 0.3878951655507734 1101 34-35 0.32023980547553776 1101 36-37 0.3566844658015995 1101 38-39 0.3848625515910342 1101 40-41 0.32676801065452565 1101 42-43 0.3405306888002073 1101 44-45 0.34582750992696987 1101 46-47 0.4314431297121786 1101 48-49 0.3915027249090599 1101 50-51 0.3860261780849754 1101 52-53 0.38596616180903 1101 54-55 0.35045585202419716 1101 56-57 0.3673824374364898 1101 58-59 0.3929314184059294 1101 60-61 0.37016567496296204 1101 62-63 0.36856349169448066 1101 64-65 0.33783312576465363 1101 66-67 0.2598286121039308 1101 68-69 0.2898317539017441 1101 70-71 0.21644916962937089 1101 72-73 0.23630865192375694 1101 74-75 0.21838628121814452 1101 76-77 0.19611659828088435 1101 78-79 0.33040073917153734 1101 80-81 0.2902083888167297 1101 82-83 0.27739745017784756 1101 84-85 0.2290903209405606 1101 86-87 0.1873853611649956 1101 88-89 0.12935200413327763 1101 90-91 0.09582975192260434 1101 92-93 0.020601472652465702 1101 94-95 0.15359371709167746 1101 96-97 0.05066382916652046 1101 98-99 -0.05008694246122403 1101 100 0.04682930609379099 1102 1 0.33281064528322446 1102 2 0.259770452743588 1102 3 0.33361744110788294 1102 4 0.23528304123279042 1102 5 0.2580178863000029 1102 6 0.18897256070948032 1102 7 0.1766504548150536 1102 8 0.190204342605945 1102 9 0.21799609775165862 1102 10-11 0.23466570338768378 1102 12-13 0.31711815935426557 1102 14-15 0.3611024822309048 1102 16-17 0.33551915678926747 1102 18-19 0.30779980519965733 1102 20-21 0.33726225838737633 1102 22-23 0.297809692224682 1102 24-25 0.3127949447387053 1102 26-27 0.29228532261417683 1102 28-29 0.3285140734151284 1102 30-31 0.37706180275380774 1102 32-33 0.4365418203101896 1102 34-35 0.422734975238896 1102 36-37 0.4127127377495512 1102 38-39 0.4003732876037418 1102 40-41 0.38598518627986067 1102 42-43 0.37105030043998255 1102 44-45 0.37989744090448596 1102 46-47 0.4402463421119194 1102 48-49 0.4109106787309429 1102 50-51 0.34047251353201347 1102 52-53 0.33122684359293686 1102 54-55 0.36978559526151145 1102 56-57 0.3762778089798573 1102 58-59 0.4404439580303503 1102 60-61 0.42670161691251707 1102 62-63 0.4172526087176678 1102 64-65 0.3261455144048284 1102 66-67 0.30227146709773933 1102 68-69 0.3205340464488202 1102 70-71 0.29840113317611383 1102 72-73 0.2991664220158512 1102 74-75 0.2828758545948915 1102 76-77 0.308995682401509 1102 78-79 0.3540677306697262 1102 80-81 0.3135648947076177 1102 82-83 0.2874468594543167 1102 84-85 0.18212468677829818 1102 86-87 0.14537114347274027 1102 88-89 0.1341045919780619 1102 90-91 0.15877448812897654 1102 92-93 0.044225905973412694 1102 94-95 0.13177664981294157 1102 96-97 0.15578936421125178 1102 98-99 0.09108332026865185 1102 100 0.20794415166440317 1103 1 0.3114530006351046 1103 2 0.26461933934513837 1103 3 0.3560738697151251 1103 4 0.1661453851289707 1103 5 0.22614705862004314 1103 6 0.20147025701950128 1103 7 0.1816804504376961 1103 8 0.21559408055517082 1103 9 0.2277982881311118 1103 10-11 0.2922247844762822 1103 12-13 0.2941318471157146 1103 14-15 0.4176245814163764 1103 16-17 0.3783235332775803 1103 18-19 0.38523356108157714 1103 20-21 0.33673048820376295 1103 22-23 0.3809975933371419 1103 24-25 0.3347388459958296 1103 26-27 0.38462645408831975 1103 28-29 0.41503941830903557 1103 30-31 0.5189693779572266 1103 32-33 0.5164467920155431 1103 34-35 0.5566591641765228 1103 36-37 0.5657507968600726 1103 38-39 0.5404234332819087 1103 40-41 0.5556440646437366 1103 42-43 0.4813591027800044 1103 44-45 0.5030753947157578 1103 46-47 0.6321040787958339 1103 48-49 0.639371163453589 1103 50-51 0.6291288506121262 1103 52-53 0.5831516789332554 1103 54-55 0.6596054949170451 1103 56-57 0.7132827084819624 1103 58-59 0.7663921308331823 1103 60-61 0.6817319688839163 1103 62-63 0.7744181144699169 1103 64-65 0.7821787771447717 1103 66-67 0.7293651642410879 1103 68-69 0.7347261434212982 1103 70-71 0.7264259137533102 1103 72-73 0.6559788560109752 1103 74-75 0.6371799146734531 1103 76-77 0.681399555853929 1103 78-79 0.732589874908868 1103 80-81 0.6238472419945609 1103 82-83 0.7271926986562285 1103 84-85 0.589842129259555 1103 86-87 0.6370457392488618 1103 88-89 0.6432724815498538 1103 90-91 0.5832726526393586 1103 92-93 0.6041073818576592 1103 94-95 0.6908551321774752 1103 96-97 0.6963834699958049 1103 98-99 0.7063444621487314 1103 100 0.8106375642206345 1104 1 0.24682622398727005 1104 2 0.1856543547599756 1104 3 0.23566707123014652 1104 4 0.061719045862382416 1104 5 0.11562282653576261 1104 6 0.15508443041581899 1104 7 0.1700064898844218 1104 8 0.18837803304534617 1104 9 0.19660160268340832 1104 10-11 0.2256957888344786 1104 12-13 0.25706835281707185 1104 14-15 0.37350235307322777 1104 16-17 0.26990257261663686 1104 18-19 0.30615853551242367 1104 20-21 0.1770951112402983 1104 22-23 0.30534801769235287 1104 24-25 0.28222182663158435 1104 26-27 0.33619866490945327 1104 28-29 0.3936480123202202 1104 30-31 0.33815569224340436 1104 32-33 0.337399319034688 1104 34-35 0.4227183044831335 1104 36-37 0.3070240002294753 1104 38-39 0.35757289406359405 1104 40-41 0.45514158964193285 1104 42-43 0.5266230824431588 1104 44-45 0.39405413224731234 1104 46-47 0.3920483949645046 1104 48-49 0.3755559493857703 1104 50-51 0.3502942775056326 1104 52-53 0.3857830225023875 1104 54-55 0.4240260708719603 1104 56-57 0.5307565812544013 1104 58-59 0.47079093850765474 1104 60-61 0.43601717042290034 1104 62-63 0.48887955879104794 1104 64-65 0.6156268452749742 1104 66-67 0.5313381445638541 1104 68-69 0.4889076868152813 1104 70-71 0.4127934392458954 1104 72-73 0.5149482859075505 1104 74-75 0.5744058200301652 1104 76-77 0.4951990269853255 1104 78-79 0.5964794308541954 1104 80-81 0.6271447061990116 1104 82-83 0.5600868385437572 1104 84-85 0.644152145484842 1104 86-87 0.6310693793248277 1104 88-89 0.5612721490823276 1104 90-91 0.49984847116427034 1104 92-93 0.5161090424036132 1104 94-95 0.5213010543860079 1104 96-97 0.5958547637653488 1104 98-99 0.5913088856599522 1104 100 0.7389900146233153 1105 1 0.20608621458517717 1105 2 0.1098325495693473 1105 3 0.20364409205036083 1105 4 0.1139110184886789 1105 5 0.10302899253510134 1105 6 0.14822854884993575 1105 7 0.16034599312928322 1105 8 0.17011491456290173 1105 9 0.1745368909016065 1105 10-11 0.21018011700626715 1105 12-13 0.21047204697009647 1105 14-15 0.31049479929841084 1105 16-17 0.30127539184665864 1105 18-19 0.25618572199354617 1105 20-21 0.26712536156633604 1105 22-23 0.3506019700530274 1105 24-25 0.3196676813363979 1105 26-27 0.3164822556985598 1105 28-29 0.37568894273426423 1105 30-31 0.4102155307004409 1105 32-33 0.4551334885752212 1105 34-35 0.46508691716892514 1105 36-37 0.4686138786160612 1105 38-39 0.4723504846778255 1105 40-41 0.4495280809085287 1105 42-43 0.40351800617580835 1105 44-45 0.46945403022105126 1105 46-47 0.5442728545179278 1105 48-49 0.5221065318327192 1105 50-51 0.5529238786959567 1105 52-53 0.5246724998535583 1105 54-55 0.5430872089632146 1105 56-57 0.4756902183990164 1105 58-59 0.5740271704223758 1105 60-61 0.6209726518904617 1105 62-63 0.5424433619831177 1105 64-65 0.6112798867217464 1105 66-67 0.6336367810771577 1105 68-69 0.6492915048171781 1105 70-71 0.684688298910352 1105 72-73 0.6576638124631131 1105 74-75 0.6667167692059905 1105 76-77 0.6176280660412488 1105 78-79 0.6769299303382041 1105 80-81 0.6783410006494179 1105 82-83 0.6984585135316941 1105 84-85 0.7336703574416283 1105 86-87 0.6467834053991943 1105 88-89 0.6319474790438449 1105 90-91 0.6877970654232755 1105 92-93 0.663198868985674 1105 94-95 0.7552645982026434 1105 96-97 0.7635693178701324 1105 98-99 0.8197478519562758 1105 100 0.9216229565017002 1106 1 0.2194079448213273 1106 2 0.1803438961363497 1106 3 0.17662081592369105 1106 4 0.15127696140415736 1106 5 0.12588430706337306 1106 6 0.08807297679238246 1106 7 0.13270211853814118 1106 8 0.10941099062655013 1106 9 0.09567586757201951 1106 10-11 0.1108568922551072 1106 12-13 0.14827864268411872 1106 14-15 0.20080042452003255 1106 16-17 0.190771053873668 1106 18-19 0.2006123844947041 1106 20-21 0.2507417749396339 1106 22-23 0.19511120825858086 1106 24-25 0.2628916187292276 1106 26-27 0.27153548530075255 1106 28-29 0.27841120494425553 1106 30-31 0.32143009136127176 1106 32-33 0.3542445003566499 1106 34-35 0.36488026433870857 1106 36-37 0.357216307460547 1106 38-39 0.3321480148691407 1106 40-41 0.377440189663794 1106 42-43 0.34237062148239517 1106 44-45 0.37121273551996836 1106 46-47 0.41441136349371277 1106 48-49 0.42587884282822586 1106 50-51 0.4316861376256398 1106 52-53 0.483079478492094 1106 54-55 0.4099014722828187 1106 56-57 0.4610785519434515 1106 58-59 0.5557581416632331 1106 60-61 0.554389636358195 1106 62-63 0.5830593590945483 1106 64-65 0.5683487329474417 1106 66-67 0.5626960429022887 1106 68-69 0.5408084786475484 1106 70-71 0.5445508392861313 1106 72-73 0.558859582993577 1106 74-75 0.6096487699181168 1106 76-77 0.5293954848185507 1106 78-79 0.6250308645429214 1106 80-81 0.6194773023467022 1106 82-83 0.5891551319320492 1106 84-85 0.5944349526573838 1106 86-87 0.4864628155053836 1106 88-89 0.49695790423202624 1106 90-91 0.5778149959924903 1106 92-93 0.5810259224315786 1106 94-95 0.6711791060377479 1106 96-97 0.6943715689398751 1106 98-99 0.678387124944841 1106 100 0.7308447502464475 1107 1 0.25962099648440073 1107 2 0.17915213102956073 1107 3 0.2562523747156433 1107 4 0.1639676520923672 1107 5 0.15093680800214315 1107 6 0.16093865544610964 1107 7 0.19396486226739285 1107 8 0.17569844019339342 1107 9 0.2453594765266658 1107 10-11 0.22277745889304867 1107 12-13 0.23949602551955707 1107 14-15 0.2981581839892229 1107 16-17 0.30868508136356354 1107 18-19 0.21960132683341982 1107 20-21 0.26033674022574615 1107 22-23 0.2728746310468324 1107 24-25 0.27875025204232173 1107 26-27 0.3445554203559169 1107 28-29 0.30139608078646063 1107 30-31 0.24978967585325762 1107 32-33 0.2680571478927689 1107 34-35 0.32407319112439836 1107 36-37 0.4494535711753045 1107 38-39 0.4848415363484193 1107 40-41 0.4386549091983696 1107 42-43 0.42762070088515003 1107 44-45 0.44175857158966636 1107 46-47 0.4645782953876534 1107 48-49 0.6025326120606564 1107 50-51 0.4602915501643281 1107 52-53 0.5125967820901991 1107 54-55 0.5145007530261623 1107 56-57 0.5777741399622371 1107 58-59 0.6624455702214291 1107 60-61 0.5907341297768625 1107 62-63 0.6066910365770823 1107 64-65 0.6938264935580492 1107 66-67 0.6492673821456094 1107 68-69 0.5805361706078571 1107 70-71 0.6957649674355721 1107 72-73 0.6082024010967473 1107 74-75 0.6795209232051462 1107 76-77 0.5814898980073515 1107 78-79 0.6823706157145182 1107 80-81 0.641982246409107 1107 82-83 0.6558602730952678 1107 84-85 0.5569141507887672 1107 86-87 0.6618984554337217 1107 88-89 0.6314815311019188 1107 90-91 0.5554309653761109 1107 92-93 0.5458028378990676 1107 94-95 0.7288308852385299 1107 96-97 0.7782053223082919 1107 98-99 0.7944355494617668 1107 100 0.8475184197953247 1108 1 0.21902566725341188 1108 2 0.15923809096474173 1108 3 0.21409005120813873 1108 4 0.13124465604008861 1108 5 0.1693347700986294 1108 6 0.13948788705588555 1108 7 0.18453179074081305 1108 8 0.17759833096950928 1108 9 0.19570724244186266 1108 10-11 0.1730048561069779 1108 12-13 0.20185046801668705 1108 14-15 0.21029828562720354 1108 16-17 0.29922110670795377 1108 18-19 0.31087101041781295 1108 20-21 0.2930706407092103 1108 22-23 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70-71 -0.48229200711383413 2316 72-73 -0.46256406130338235 2316 74-75 -0.45613955289104524 2316 76-77 -0.5092079169492791 2316 78-79 -0.3238429486808698 2316 80-81 -0.22921891991596866 2316 82-83 -0.10837780328481372 2316 84-85 -0.17405540595369828 2316 86-87 -0.20792206345300457 2316 88-89 -0.22980696495461217 2316 90-91 -0.17765079567061548 2316 92-93 -0.07234022882485647 2316 94-95 -0.08856910087511949 2316 96-97 -0.05654824957030158 2316 98-99 -0.16829481292061743 2316 100 -0.34408303672881857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 13.0 11 31.0 12 89.0 13 206.0 14 681.0 15 1708.0 16 3879.0 17 7788.0 18 13885.0 19 21473.0 20 30686.0 21 42459.0 22 55168.0 23 64908.0 24 71353.0 25 79531.0 26 94042.0 27 112875.0 28 137663.0 29 166035.0 30 200305.0 31 243876.0 32 297359.0 33 375770.0 34 493393.0 35 661879.0 36 978656.0 37 1547877.0 38 1556935.0 39 238558.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15642803925336 20.0454521632784 13.713233975292022 24.084885822176215 2 15.031781143859632 21.361841997280724 40.01205512370113 23.594321735158513 3 17.268533532753988 26.946685022754863 30.744683064108337 25.04009838038281 4 12.109736347131275 17.418118125392965 37.94494380299411 32.52720172448165 5 12.344419255900787 38.478503800506736 35.385238031737565 13.791838911854914 6 30.621725259725608 37.8501968511287 18.04833613990432 13.479741749241372 7 26.84439430519468 33.07391605542787 22.675142333462013 17.40654730591543 8 25.62479622405253 36.707340734720226 20.306452354933192 17.361410686294054 9 26.835223467732323 15.215637473973592 20.1775303055801 37.77160875271399 10-11 24.587107945404224 26.49701998169117 29.107081302260333 19.80879077064427 12-13 25.032433827191607 24.24860800241095 28.990800914507915 21.728157255889528 14-15 22.424612770973642 25.693758530173227 26.48183819023978 25.39979050861335 16-17 22.056752971228928 28.588245954300707 27.172972126378518 22.182028948091848 18-19 21.94233511762946 27.781672723112194 28.907984233439088 21.36800792581926 20-21 22.707093662676584 27.127498380258874 28.758521185187103 21.40688677187744 22-23 22.452825760483172 26.95006370085853 28.6702535792895 21.926856959368802 24-25 22.23679727541183 26.989464756841308 28.755639832166864 22.018098135579997 26-27 22.094401017401612 27.148598495806866 28.751970152165523 22.005030334626 28-29 22.110563686470567 27.32313484628427 28.789733264337958 21.776568202907207 30-31 22.594944739488255 27.030014279941124 28.846787906702787 21.52825307386784 32-33 22.34413281694597 27.116404189018578 28.7622605604761 21.777202433559356 34-35 22.384479424291428 27.31148502012457 28.73106662217951 21.57296893340449 36-37 22.418090573282477 27.27365830601802 28.658456371413283 21.649794749286222 38-39 22.305224239798445 27.452067201630292 28.550158157448557 21.692550401122705 40-41 22.389585834735964 27.185137656019347 28.687976244445796 21.737300264798897 42-43 22.193142667621423 27.306473026616416 28.471785982598185 22.028598323163973 44-45 22.112358837330223 27.50128122088248 28.466787674195032 21.919572267592265 46-47 22.108129524772753 27.300865323719247 28.354231287226213 22.23677386428179 48-49 22.115619023741 27.377227469253523 28.41315237076611 22.09400113623937 50-51 22.224405634385846 27.583028081643995 28.251238888213937 21.941327395756222 52-53 22.258754624476044 27.607147655515252 28.068066686469777 22.066031033538934 54-55 22.121945800858736 27.509048614617164 28.297353571932454 22.07165201259165 56-57 22.3016084104316 27.505633973827315 27.9461570861052 22.24660052963588 58-59 22.131358228310365 27.555184774037123 28.18415907825113 22.129297919401385 60-61 22.441730284648933 27.642079419513276 28.077977898349477 21.83821239748831 62-63 22.234946300912938 27.70758195566637 28.121257143123877 21.93621460029681 64-65 22.05961738340046 27.447662861695214 28.10746966242269 22.385250092481638 66-67 22.146472042048817 27.476764165683782 27.976568655535655 22.400195136731742 68-69 21.96664418548482 27.52544870229523 28.185033774452616 22.322873337767334 70-71 22.072812863605904 27.390592337979708 28.15993492183619 22.376659876578195 72-73 21.947254335260116 27.768522608218802 27.811977896414646 22.472245160106432 74-75 21.949608705656548 27.712378137870857 27.816866872843725 22.521146283628873 76-77 21.799398606010925 27.571503382291105 27.990937617475392 22.638160394222577 78-79 21.765324398914103 27.490835990145484 27.994110548768376 22.74972906217204 80-81 21.80523742413299 27.707594755747973 27.74030065153078 22.74686716858826 82-83 21.91072470896748 27.6565067721923 27.81084475035812 22.621923768482098 84-85 21.74677006857413 27.743483637230316 27.726832082777364 22.78291421141819 86-87 21.56578056880243 27.779249405724627 27.783951056944534 22.871018968528414 88-89 21.457931350267636 27.561613988087043 27.905016701977488 23.075437959667838 90-91 21.817389405097547 27.47748754124436 27.849904714903325 22.855218338754774 92-93 21.695013650359247 27.500271459797403 27.78191600193477 23.02279888790858 94-95 21.48461238464966 27.419921682064718 27.783530662274064 23.311935271011553 96-97 21.37379696098644 27.345760184875573 27.92076871787122 23.359674136266772 98-99 21.29247209961613 27.309389417543496 27.801686956996086 23.596451525844287 100 20.526764423008313 27.856449399082724 27.980515419491063 23.636270758417904 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2316.0 1 1765.0 2 1898.0 3 2722.0 4 2249.5 5 1961.5 6 2953.0 7 5595.5 8 8899.0 9 10516.0 10 9161.5 11 7307.5 12 7255.5 13 7724.0 14 8169.0 15 8531.5 16 9052.5 17 9640.0 18 10195.0 19 10848.5 20 11942.0 21 13518.0 22 15945.5 23 19331.0 24 23780.5 25 29699.0 26 37247.5 27 46460.5 28 56729.5 29 68972.0 30 83578.0 31 98835.0 32 115903.5 33 134358.5 34 153538.0 35 171447.5 36 191847.5 37 211549.0 38 225438.0 39 237352.5 40 243537.5 41 248421.5 42 255348.0 43 264327.0 44 272205.0 45 279465.5 46 286671.0 47 291817.0 48 299064.5 49 305370.5 50 306973.0 51 296651.5 52 278597.5 53 262022.5 54 243746.0 55 219868.5 56 194728.5 57 171549.0 58 149175.0 59 126086.5 60 101909.0 61 80916.5 62 64257.5 63 49571.0 64 36191.0 65 26091.5 66 18833.5 67 13896.0 68 10359.5 69 7585.0 70 5589.5 71 4091.0 72 2880.0 73 1978.0 74 1407.0 75 947.0 76 627.0 77 403.5 78 240.0 79 155.5 80 121.0 81 88.5 82 64.0 83 48.0 84 32.0 85 26.5 86 25.0 87 16.0 88 12.0 89 8.0 90 3.5 91 3.5 92 2.5 93 4.0 94 3.0 95 4.0 96 4.0 97 3.0 98 6.0 99 5.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032270474582667425 2 0.06942152507329198 3 7.867595042881726E-4 4 6.667453426170954E-4 5 0.0015335142880193197 6 6.267406220600698E-4 7 0.0 8 9.334434796639336E-4 9 1.333490685234191E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.534458399424465E-4 18-19 0.0010601250947611818 20-21 0.003087030936317152 22-23 1.333490685234191E-5 24-25 0.0 26-27 1.5335142880193195E-4 28-29 8.200967714190275E-4 30-31 0.0017935449716399868 32-33 0.002967016774646075 34-35 0.004280505099601753 36-37 0.0015401817414454908 38-39 0.001340158138660362 40-41 0.005407304728624645 42-43 0.007047498271462699 44-45 0.005440641995755499 46-47 0.00712084025915058 48-49 0.009267760262377626 50-51 0.008774368708840977 52-53 0.0039004602543100085 54-55 0.002360278512864518 56-57 0.002526964848518792 58-59 0.0031937101911358873 60-61 0.007734245974358308 62-63 0.014435036667660118 64-65 0.004387184354420488 66-67 0.010441232065383716 68-69 0.013228227597523174 70-71 0.00586735901503044 72-73 0.008040948831962172 74-75 0.002940346960941391 76-77 0.0027203209978777496 78-79 0.00831431442243518 80-81 0.006227401500043672 82-83 0.017128687851833184 84-85 0.017555404871108127 86-87 0.023322752084746 88-89 0.02199592885293798 90-91 0.025036287615271935 92-93 0.034790771977760045 94-95 0.03834452465390916 96-97 0.023309417177893657 98-99 0.01884889083578529 100 0.019922350837398815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7499115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.338021443892785 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34582656474907 42.2051238151048 2 16.706861675573712 19.492905093795397 3 5.425860829595268 9.4960195628552 4 2.241258507413491 5.230023474671815 5 1.0805718050513649 3.151921056737623 6 0.6087080934197132 2.1306495484194192 7 0.37521178879524075 1.532237936651669 8 0.23553781802914214 1.0992648223225452 9 0.16947767154798649 0.889829283332471 >10 0.7370148734600013 7.307436797328829 >50 0.04289558630674834 1.7343475651338112 >100 0.028174924504586057 3.089248363427635 >500 0.0018937292270759787 0.7525681901511515 >1k 6.098415548184616E-4 0.7351508537912326 >5k 0.0 0.0 >10k+ 9.629077180491818E-5 1.1532736362763716 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24477 0.3263985150247729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21846 0.29131437509626135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15268 0.20359735782155627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.3334906852341909E-5 0.0 4 0.0 1.3334906852341909E-5 0.0 1.3334906852341909E-5 0.0 5 0.0 2.6669813704683818E-5 0.0 1.3334906852341909E-5 0.0 6 0.0 2.6669813704683818E-5 0.0 1.3334906852341909E-5 0.0 7 0.0 2.6669813704683818E-5 0.0 1.3334906852341909E-5 0.0 8 0.0 2.6669813704683818E-5 0.0 1.3334906852341909E-5 0.0 9 0.0 2.6669813704683818E-5 0.0 4.000472055702573E-5 2.6669813704683818E-5 10-11 0.0 2.6669813704683818E-5 0.0 5.3339627409367636E-5 2.6669813704683818E-5 12-13 0.0 4.000472055702573E-5 0.0 6.000708083553859E-5 2.6669813704683818E-5 14-15 0.0 1.1334670824490623E-4 0.0 7.33419876878805E-5 2.6669813704683818E-5 16-17 0.0 1.600188822281029E-4 0.0 8.000944111405145E-5 2.6669813704683818E-5 18-19 0.0 1.600188822281029E-4 0.0 8.000944111405145E-5 4.667217398319668E-5 20-21 0.0 1.7335378908044482E-4 0.0 1.0001180139256432E-4 6.667453426170954E-5 22-23 0.0 1.7335378908044482E-4 0.0 1.5335142880193195E-4 7.33419876878805E-5 24-25 0.0 1.8668869593278673E-4 0.0 2.066910562112996E-4 9.334434796639336E-5 26-27 0.0 2.0002360278512863E-4 0.0 3.267052178823768E-4 9.334434796639336E-5 28-29 0.0 2.1335850963747054E-4 0.0 9.801156536471303E-4 9.334434796639336E-5 30-31 0.0 2.2669341648981245E-4 0.0 0.004593875410631788 1.0001180139256432E-4 32-33 0.0 2.4002832334215436E-4 0.0 0.012701498776855669 1.1334670824490623E-4 34-35 0.0 2.93367950751522E-4 0.0 0.024629572956275506 1.2001416167107718E-4 36-37 0.0 3.533750315870606E-4 0.0 0.04533201584453632 1.2001416167107718E-4 38-39 0.0 4.467193795534539E-4 0.0 0.08022946707711509 1.333490685234191E-4 40-41 0.0 4.800566466843087E-4 0.0 0.12383461248427315 1.333490685234191E-4 42-43 0.0 5.467311809460183E-4 0.0 0.1640860288180672 1.46683975375761E-4 44-45 0.0 5.600660877983602E-4 0.0 0.21499203572688244 1.46683975375761E-4 46-47 0.0 5.734009946507021E-4 0.0 0.2755591826502194 1.600188822281029E-4 48-49 0.0 6.200731686338988E-4 0.0 0.3440139269767166 1.600188822281029E-4 50-51 0.0 6.467429823385826E-4 0.0 0.41930947851846523 1.600188822281029E-4 52-53 0.0 6.667453426170954E-4 0.0 0.4955984806207132 1.600188822281029E-4 54-55 0.0 6.800802494694374E-4 0.0 0.5830888044789285 1.9335614935895768E-4 56-57 0.0 6.800802494694374E-4 0.0 0.6851408466199012 2.4002832334215436E-4 58-59 0.0 6.800802494694374E-4 0.0 0.8052283502786661 2.600306836206672E-4 60-61 0.0 7.067500631741212E-4 0.0 0.9334301447570814 2.7336559047300916E-4 62-63 0.0 7.067500631741212E-4 0.0 1.0681860459534225 2.800330438991801E-4 64-65 0.0 7.134175166002921E-4 0.0 1.2053222280229066 2.800330438991801E-4 66-67 0.0 7.534222371573179E-4 0.0 1.367968086900921 2.800330438991801E-4 68-69 0.0 7.600896905834889E-4 0.0 1.5602307738979868 2.800330438991801E-4 70-71 0.0 7.667571440096597E-4 0.0 1.7693821204235434 2.800330438991801E-4 72-73 0.0 7.867595042881726E-4 0.0 1.9734928721589147 3.200377644562058E-4 74-75 0.0 8.000944111405145E-4 0.0 2.178603741908212 3.4670757816088963E-4 76-77 0.0 8.000944111405145E-4 0.0 2.4066506514435373 3.6004248501323154E-4 78-79 0.0 8.400991316975402E-4 0.0 2.676222460917055 3.6004248501323154E-4 80-81 0.0 8.534340385498822E-4 0.0 2.964789845201734 3.7337739186557345E-4 82-83 0.0 8.534340385498822E-4 0.0 3.269339115348944 4.0004720557025727E-4 84-85 0.0 8.667689454022241E-4 0.0 3.5693678520732113 4.0671465899642825E-4 86-87 0.0 8.667689454022241E-4 0.0 3.8743238368794186 4.200495658487701E-4 88 0.0 8.667689454022241E-4 0.0 4.130207364469007 4.267170192749411E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13185 0.0 43.297592 1 GTATCAA 21650 0.0 35.860336 1 TACCTGG 6285 0.0 33.81813 2 GTACCTG 7240 0.0 29.68266 1 TCAACGC 26465 0.0 28.676203 4 ATCAACG 26690 0.0 28.399437 3 CAACGCA 26820 0.0 28.384241 5 AACGCAG 27825 0.0 27.477259 6 TATCAAC 27970 0.0 27.370228 2 GTACATG 27380 0.0 27.29076 1 CATGGGG 16035 0.0 26.316515 4 TACATGG 28200 0.0 26.129818 2 ACCTGGG 8020 0.0 25.781147 3 ACATGGG 27790 0.0 25.246151 3 ATGGGGG 10225 0.0 25.046913 5 ACGCAGA 31480 0.0 24.197273 7 CGCAGAG 32185 0.0 23.419035 8 TATAACG 605 0.0 23.31752 2 TGGGGGG 9790 0.0 23.087847 6 GCAGAGT 36130 0.0 20.289526 9 >>END_MODULE