FastQCFastQC Report
Mon 6 Jun 2016
ERR1211505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432862
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270800.23686107643038112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247280.2162887997773436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152350.1332562222827495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162300.046.388231
GTATCAA273700.036.0573961
TCAACGC313900.030.5525154
CAACGCA317000.030.1935215
ATCAACG318450.030.1174343
AACGCAG328600.029.2057786
GTACATG343750.028.6136681
TATCAAC344200.027.9926362
TACATGG345750.027.581542
ACATGGG343050.026.6427083
TACCTGG93650.025.8088152
ACGCAGA372200.025.4449237
CGCAGAG384000.024.4318668
CATGGGG185850.024.1454934
GTACCTG104300.024.0613941
GCAGAGT429400.021.0192369
GAGTACT260200.019.83598712-13
ACCTGGG121800.019.1745553
ATGGGGG115150.019.0153335
CAGAGTA417950.019.00896610-11
Produced by FastQC (version 0.11.5)