FastQCFastQC Report
Mon 6 Jun 2016
ERR1211505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11432862
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306050.2676932512611453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289640.25333988987184486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186390.16303004444556402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178950.047.042891
GTATCAA288900.034.5275541
TCAACGC344100.028.0773284
ATCAACG349300.027.6731623
CAACGCA351400.027.4670645
GTACATG350150.027.2118471
TACCTGG95300.026.6479642
AACGCAG365200.026.5187596
TATCAAC375550.025.9719282
TACATGG362550.025.5671252
ACATGGG349250.025.0399253
GTACCTG104100.024.6669031
ACGCAGA418550.023.0598857
CGCAGAG426500.022.4207128
CATGGGG191650.022.0673924
ACCTGGG121700.020.3103873
GAGTACT297000.019.3977512-13
GCAGAGT484750.019.2902329
ATGGGGG114000.018.8375725
AGAGTAC442600.018.5422810-11
Produced by FastQC (version 0.11.5)