##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211505_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11432862 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13874120058477 33.0 31.0 34.0 30.0 34.0 2 32.35721676689529 34.0 31.0 34.0 31.0 34.0 3 32.374410187055524 34.0 31.0 34.0 30.0 34.0 4 36.00273308643103 37.0 35.0 37.0 35.0 37.0 5 35.92926862932484 37.0 35.0 37.0 35.0 37.0 6 35.94391369370154 37.0 35.0 37.0 35.0 37.0 7 35.86521957494108 37.0 35.0 37.0 35.0 37.0 8 35.859837195620834 37.0 35.0 37.0 35.0 37.0 9 37.515497344409475 39.0 37.0 39.0 35.0 39.0 10-11 37.479563603584126 39.0 37.0 39.0 34.5 39.0 12-13 37.401115180083515 39.0 37.0 39.0 34.0 39.0 14-15 38.65617830426012 40.0 38.0 41.0 34.0 41.0 16-17 38.5877473199624 40.0 38.0 41.0 34.0 41.0 18-19 38.567490318697104 40.0 38.0 41.0 34.0 41.0 20-21 38.5042048526432 40.0 38.0 41.0 34.0 41.0 22-23 38.40511159847814 40.0 38.0 41.0 34.0 41.0 24-25 38.30335531907933 40.0 38.0 41.0 34.0 41.0 26-27 38.137041801081836 40.0 38.0 41.0 33.0 41.0 28-29 38.17876232565389 40.0 38.0 41.0 33.5 41.0 30-31 38.117542134244246 40.0 38.0 41.0 33.0 41.0 32-33 37.9550252596419 40.0 37.0 41.0 33.0 41.0 34-35 37.8540855736735 40.0 37.0 41.0 33.0 41.0 36-37 37.80420786151359 40.0 37.0 41.0 33.0 41.0 38-39 37.71663945563237 40.0 37.0 41.0 33.0 41.0 40-41 37.56327308945039 40.0 37.0 41.0 32.0 41.0 42-43 37.42501230225643 40.0 37.0 41.0 31.5 41.0 44-45 37.253277963120695 40.0 36.0 41.0 31.0 41.0 46-47 37.01717732620231 39.5 36.0 41.0 30.5 41.0 48-49 36.818135957558134 39.0 36.0 41.0 30.0 41.0 50-51 36.631361552339214 39.0 35.5 41.0 30.0 41.0 52-53 36.443172322031 39.0 35.0 40.5 29.5 41.0 54-55 36.25587005248555 39.0 35.0 40.0 29.0 41.0 56-57 36.06290703937474 38.5 35.0 40.0 28.0 41.0 58-59 35.80876468201925 38.0 34.5 40.0 28.0 41.0 60-61 35.5052981921762 38.0 34.0 40.0 27.5 41.0 62-63 35.18930120034686 38.0 34.0 40.0 26.5 41.0 64-65 34.87756381560453 37.0 34.0 40.0 26.0 41.0 66-67 34.53932991581635 36.5 33.0 39.5 26.0 41.0 68-69 34.14437076210663 36.0 33.0 39.0 26.0 40.5 70-71 33.75885972383818 35.5 32.0 39.0 25.5 40.0 72-73 33.35134999442835 35.0 32.0 38.0 25.0 40.0 74-75 32.9420541418238 35.0 31.5 37.0 24.0 39.5 76-77 31.38775408992079 33.5 29.5 35.5 22.5 38.5 78-79 32.20929037716016 34.5 31.0 36.0 24.0 39.0 80-81 32.16957245701033 35.0 31.0 36.0 24.0 38.0 82-83 31.879469025341162 34.0 31.0 35.5 23.0 37.0 84-85 31.576420147466138 34.0 31.0 35.0 22.5 37.0 86-87 31.282694175789054 34.0 31.0 35.0 21.0 36.0 88-89 31.003388171745623 34.0 30.0 35.0 20.0 36.0 90-91 30.722451123786854 34.0 30.0 35.0 20.0 36.0 92-93 30.515048725332292 34.0 29.5 35.0 19.5 35.0 94-95 30.297085191791872 34.0 29.0 35.0 19.0 35.0 96-97 30.093108707163612 34.0 29.0 35.0 18.5 35.0 98-99 29.934939081745235 34.0 29.0 35.0 18.0 35.0 100 28.051399378388368 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2847796439831285 1101 2 0.2185237975958927 1101 3 0.27135974309985755 1101 4 0.1607145256718141 1101 5 0.1400495395027619 1101 6 0.17289826000669706 1101 7 0.2043271903612478 1101 8 0.14860830033221362 1101 9 0.16573399804883593 1101 10-11 0.22373365017824653 1101 12-13 0.22200960196491337 1101 14-15 0.2729433440653324 1101 16-17 0.2949766626597423 1101 18-19 0.30507849664620323 1101 20-21 0.2778426365822213 1101 22-23 0.2559028071580087 1101 24-25 0.18285241776224126 1101 26-27 0.19320333820564173 1101 28-29 0.230900602147166 1101 30-31 0.26003202051229124 1101 32-33 0.27347275427876383 1101 34-35 0.25913294688817956 1101 36-37 0.30951055729558163 1101 38-39 0.32224333265199334 1101 40-41 0.3267899664837657 1101 42-43 0.29488873856734443 1101 44-45 0.2412886107892689 1101 46-47 0.2674178488633956 1101 48-49 0.29808142005257565 1101 50-51 0.27219856452914115 1101 52-53 0.30960190358892703 1101 54-55 0.2328276305816459 1101 56-57 0.2136447566801678 1101 58-59 0.2092205813839243 1101 60-61 0.15510223376082877 1101 62-63 0.13502889115638794 1101 64-65 0.22187608784412305 1101 66-67 0.14925514072930923 1101 68-69 0.17795703457212397 1101 70-71 0.14439759757939186 1101 72-73 0.0755358439840137 1101 74-75 0.173393210774357 1101 76-77 0.14199320842498864 1101 78-79 0.24238501981602667 1101 80-81 0.18198578177440794 1101 82-83 0.1545186349024128 1101 84-85 0.10841264053444988 1101 86-87 0.049306187803068724 1101 88-89 0.010808557924313789 1101 90-91 -0.013919208959663365 1101 92-93 0.019165018192609296 1101 94-95 0.03806445992077201 1101 96-97 0.022363463350252744 1101 98-99 -0.07154273601290839 1101 100 0.010999537878049637 1102 1 0.3176347539804425 1102 2 0.24657795052397802 1102 3 0.3060250631686898 1102 4 0.1844970789858067 1102 5 0.21319033651209907 1102 6 0.16627336843441753 1102 7 0.20072554109017915 1102 8 0.18018878322845922 1102 9 0.23547641829527066 1102 10-11 0.19896975284718366 1102 12-13 0.2183910683416599 1102 14-15 0.3087705009328445 1102 16-17 0.30073710369787676 1102 18-19 0.31047877747354846 1102 20-21 0.3437531813978154 1102 22-23 0.3046089045409204 1102 24-25 0.2623456928851198 1102 26-27 0.2610179474526788 1102 28-29 0.32958506586429337 1102 30-31 0.3735048185946752 1102 32-33 0.45033103759102744 1102 34-35 0.4008578409883867 1102 36-37 0.43237055998199025 1102 38-39 0.40913136449493237 1102 40-41 0.42467614934866305 1102 42-43 0.3655229638849633 1102 44-45 0.3868219874748249 1102 46-47 0.45110160796538423 1102 48-49 0.43626687287203225 1102 50-51 0.42576285599191976 1102 52-53 0.4407736766873711 1102 54-55 0.46124657512664413 1102 56-57 0.4396045519248588 1102 58-59 0.4704790249748996 1102 60-61 0.5456813264806328 1102 62-63 0.5236982363038578 1102 64-65 0.45707289713545407 1102 66-67 0.4343159460921626 1102 68-69 0.4325596712476525 1102 70-71 0.44415184536169505 1102 72-73 0.47789436631833127 1102 74-75 0.4863598178647308 1102 76-77 0.47140392472523374 1102 78-79 0.4498887560684892 1102 80-81 0.39997111634679783 1102 82-83 0.34058412524852955 1102 84-85 0.2836019669450103 1102 86-87 0.26857963809308316 1102 88-89 0.285051917280299 1102 90-91 0.35040497550726 1102 92-93 0.22670167421553344 1102 94-95 0.28606668261840085 1102 96-97 0.3544105439042724 1102 98-99 0.27779519458051993 1102 100 0.2816384417647697 1103 1 0.29457879064817405 1103 2 0.27946702359436415 1103 3 0.324469739768368 1103 4 0.1395371568048418 1103 5 0.1893875115554451 1103 6 0.17672566853023852 1103 7 0.188600063069579 1103 8 0.2068332756842608 1103 9 0.2922088588280687 1103 10-11 0.2446137004719091 1103 12-13 0.27004467815795863 1103 14-15 0.3643988754379919 1103 16-17 0.3761711624880846 1103 18-19 0.38282355141285507 1103 20-21 0.3802774991878479 1103 22-23 0.3437102935403331 1103 24-25 0.3524812779360573 1103 26-27 0.36571300713671206 1103 28-29 0.4067061420599387 1103 30-31 0.4231469298602448 1103 32-33 0.47967518119314434 1103 34-35 0.4536688170581513 1103 36-37 0.4892853477245609 1103 38-39 0.4905569307119535 1103 40-41 0.546165942043821 1103 42-43 0.551309466861504 1103 44-45 0.4835241885660295 1103 46-47 0.5437617753203341 1103 48-49 0.5256870849385038 1103 50-51 0.5431919193057695 1103 52-53 0.5115362816910505 1103 54-55 0.5547895061597998 1103 56-57 0.649540948634872 1103 58-59 0.6189933255677644 1103 60-61 0.572792445775498 1103 62-63 0.6067100633335372 1103 64-65 0.6018520782859582 1103 66-67 0.6051079303025944 1103 68-69 0.5948117317193891 1103 70-71 0.6321747707847507 1103 72-73 0.5537385769896019 1103 74-75 0.5542824015266774 1103 76-77 0.5505677999792375 1103 78-79 0.5687200045199248 1103 80-81 0.5704217698148639 1103 82-83 0.5373056757444594 1103 84-85 0.4663695112675619 1103 86-87 0.5073034126887919 1103 88-89 0.44950491374773804 1103 90-91 0.44320138590578395 1103 92-93 0.3739501032639829 1103 94-95 0.4978713856995718 1103 96-97 0.5423584280652065 1103 98-99 0.516585850200812 1103 100 0.5542019453446443 1104 1 0.2860904338744419 1104 2 0.2601189666321275 1104 3 0.2732165550580987 1104 4 0.11272174418756009 1104 5 0.16970430973654516 1104 6 0.17447600265886365 1104 7 0.18823371462546135 1104 8 0.18284612286851853 1104 9 0.2455194278755286 1104 10-11 0.2881377031149057 1104 12-13 0.29643918010934556 1104 14-15 0.3876000732093061 1104 16-17 0.2750847530813729 1104 18-19 0.32498403071124216 1104 20-21 0.264399203024027 1104 22-23 0.30290275350828466 1104 24-25 0.3233223184732523 1104 26-27 0.40247002839619483 1104 28-29 0.440966162611538 1104 30-31 0.3764335792902216 1104 32-33 0.3683595138836324 1104 34-35 0.5041263073512567 1104 36-37 0.39721411996748657 1104 38-39 0.4539034093788814 1104 40-41 0.5464416168102346 1104 42-43 0.58125971511447 1104 44-45 0.5287627613740895 1104 46-47 0.49792774677905527 1104 48-49 0.544531625728716 1104 50-51 0.5262836574534404 1104 52-53 0.5144608455615867 1104 54-55 0.5497367659878662 1104 56-57 0.6573600378007427 1104 58-59 0.6322659847236238 1104 60-61 0.5863766415015021 1104 62-63 0.5410346915238762 1104 64-65 0.6391182020524937 1104 66-67 0.6312840864430882 1104 68-69 0.5819666856566172 1104 70-71 0.5217988912659308 1104 72-73 0.6258591754950942 1104 74-75 0.6672898619097012 1104 76-77 0.5659230724421214 1104 78-79 0.6356564136294054 1104 80-81 0.7188442563559079 1104 82-83 0.5233758262728649 1104 84-85 0.5497547634464759 1104 86-87 0.6757422251911578 1104 88-89 0.6663329391464288 1104 90-91 0.537585444476985 1104 92-93 0.5814658432769129 1104 94-95 0.6238141751907982 1104 96-97 0.547230681447548 1104 98-99 0.5747467613916193 1104 100 0.7498648746063239 1105 1 0.2561464639830504 1105 2 0.15662718367720885 1105 3 0.23437879921013405 1105 4 0.12260273184153192 1105 5 0.11813834472429363 1105 6 0.10148444290668124 1105 7 0.14492771226044 1105 8 0.13472662435076188 1105 9 0.15138329935339812 1105 10-11 0.1871890575544839 1105 12-13 0.2403227205472973 1105 14-15 0.2579023754623009 1105 16-17 0.26120494694838214 1105 18-19 0.2955210446116254 1105 20-21 0.24508065679049906 1105 22-23 0.2953375116288939 1105 24-25 0.3399022162915699 1105 26-27 0.3430829970385574 1105 28-29 0.3815076118698997 1105 30-31 0.3650890071039399 1105 32-33 0.4159788866132459 1105 34-35 0.4405637505333644 1105 36-37 0.4411042304058128 1105 38-39 0.43294920519853264 1105 40-41 0.43050780302119307 1105 42-43 0.44684904559988325 1105 44-45 0.4330888667840611 1105 46-47 0.44985327656901575 1105 48-49 0.5907450563261207 1105 50-51 0.5496706116383265 1105 52-53 0.5572543659605458 1105 54-55 0.5045029389870805 1105 56-57 0.5535877891727949 1105 58-59 0.5380003742477086 1105 60-61 0.5183214400513094 1105 62-63 0.5296097626775946 1105 64-65 0.5396733667869427 1105 66-67 0.5793662312388861 1105 68-69 0.6086802383661407 1105 70-71 0.599670100748213 1105 72-73 0.5315530428032247 1105 74-75 0.5698447797696033 1105 76-77 0.5672333223796819 1105 78-79 0.6402370011643654 1105 80-81 0.6472781987316196 1105 82-83 0.6390513253873031 1105 84-85 0.6430295554934524 1105 86-87 0.6044076324565637 1105 88-89 0.6378861775325717 1105 90-91 0.711705958679687 1105 92-93 0.6884767060233834 1105 94-95 0.830066174139052 1105 96-97 0.769105508040834 1105 98-99 0.7610922995713238 1105 100 0.7990877904853768 1106 1 0.19980316636812034 1106 2 0.13642897464087156 1106 3 0.20477135526360257 1106 4 0.13879592548577335 1106 5 0.12564745434746527 1106 6 0.08944142761623652 1106 7 0.11645219659136785 1106 8 0.13452354347376883 1106 9 0.13659789853809912 1106 10-11 0.1506387910297704 1106 12-13 0.19180122350969953 1106 14-15 0.22388972964439802 1106 16-17 0.2680797660718355 1106 18-19 0.24725568164770095 1106 20-21 0.2779991090960934 1106 22-23 0.2886534637129543 1106 24-25 0.26801813147861964 1106 26-27 0.2757232363286306 1106 28-29 0.3137036528592958 1106 30-31 0.3289205093304375 1106 32-33 0.3330352624067032 1106 34-35 0.34261656159260667 1106 36-37 0.3291770895739745 1106 38-39 0.3164906966822656 1106 40-41 0.3421370238986654 1106 42-43 0.3630352916292665 1106 44-45 0.459621868929446 1106 46-47 0.47544040781662744 1106 48-49 0.43458380179269085 1106 50-51 0.4625108064349348 1106 52-53 0.510334852144382 1106 54-55 0.41600645577725714 1106 56-57 0.46609544484262244 1106 58-59 0.5022647368420152 1106 60-61 0.5398760364471826 1106 62-63 0.490015636894114 1106 64-65 0.516860226646024 1106 66-67 0.5634444478504363 1106 68-69 0.5452372611762257 1106 70-71 0.5035921497527198 1106 72-73 0.5353636052528685 1106 74-75 0.5491929055453184 1106 76-77 0.47700250059574145 1106 78-79 0.5743286799491187 1106 80-81 0.5963642590379905 1106 82-83 0.5746423907195712 1106 84-85 0.5574319617208836 1106 86-87 0.5376133047498399 1106 88-89 0.5871746879924942 1106 90-91 0.6675063325737511 1106 92-93 0.6610626405683284 1106 94-95 0.7362665712485672 1106 96-97 0.7385412204193322 1106 98-99 0.7195820812389471 1106 100 0.8343441384316357 1107 1 0.21329109046812533 1107 2 0.17228528472097793 1107 3 0.2029770895624594 1107 4 0.14148332669923036 1107 5 0.1399269071989906 1107 6 0.12180818534722704 1107 7 0.13482403138815613 1107 8 0.13856493921463908 1107 9 0.2178921075111191 1107 10-11 0.175188099972722 1107 12-13 0.2077152158625637 1107 14-15 0.24438989916562548 1107 16-17 0.21709901541223786 1107 18-19 0.20809020143130397 1107 20-21 0.20663193642899103 1107 22-23 0.2777521428294065 1107 24-25 0.28021605874655364 1107 26-27 0.27075214027589567 1107 28-29 0.2908764251254894 1107 30-31 0.18818800310649664 1107 32-33 0.21146930830555277 1107 34-35 0.26014108958550963 1107 36-37 0.3328677390244934 1107 38-39 0.29698283074792897 1107 40-41 0.32099357729583033 1107 42-43 0.3205631450357558 1107 44-45 0.32434192730571 1107 46-47 0.3842721938918743 1107 48-49 0.38598512077673774 1107 50-51 0.3777474873147142 1107 52-53 0.35729031478692264 1107 54-55 0.3649515870294877 1107 56-57 0.3736654261660064 1107 58-59 0.4675324937730849 1107 60-61 0.4645804346775648 1107 62-63 0.4241935904481764 1107 64-65 0.5133299417299497 1107 66-67 0.5157699328470144 1107 68-69 0.466501178257964 1107 70-71 0.5404086080870982 1107 72-73 0.45898802494393465 1107 74-75 0.5460711114144772 1107 76-77 0.4792447780527489 1107 78-79 0.6211691766644378 1107 80-81 0.5854311360749591 1107 82-83 0.5734799058791715 1107 84-85 0.47617221352208716 1107 86-87 0.416651664527798 1107 88-89 0.48934478281672966 1107 90-91 0.3465744684933867 1107 92-93 0.4225558479472049 1107 94-95 0.5846269433525428 1107 96-97 0.6616820582414746 1107 98-99 0.6735555045668065 1107 100 0.8310339209307571 1108 1 0.22173605549805586 1108 2 0.15066285200357754 1108 3 0.20293461688681447 1108 4 0.12358996880110595 1108 5 0.11935742270024718 1108 6 0.12572220337427353 1108 7 0.14202688506968286 1108 8 0.15737512021951972 1108 9 0.13222344667340025 1108 10-11 0.14723060106292252 1108 12-13 0.18770202459778318 1108 14-15 0.2023861786611647 1108 16-17 0.2518962371921205 1108 18-19 0.26703830079740953 1108 20-21 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2316 72-73 -0.430606068496715 2316 74-75 -0.5316515769969712 2316 76-77 -0.42087586771367214 2316 78-79 -0.29506559622184625 2316 80-81 -0.24670212746370623 2316 82-83 -0.22852270104865724 2316 84-85 -0.31217751209335987 2316 86-87 -0.20832711535539872 2316 88-89 -0.14432651993344336 2316 90-91 -0.06729847170980108 2316 92-93 -0.051863253005546284 2316 94-95 0.047081205766332346 2316 96-97 0.013559292311690285 2316 98-99 -0.1365400625658566 2316 100 -0.32275594487631665 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 8.0 10 19.0 11 38.0 12 122.0 13 374.0 14 1037.0 15 2590.0 16 5880.0 17 11852.0 18 21064.0 19 32450.0 20 46663.0 21 64730.0 22 83784.0 23 96096.0 24 104859.0 25 117696.0 26 139122.0 27 168483.0 28 204612.0 29 248707.0 30 300120.0 31 364078.0 32 446193.0 33 559164.0 34 731337.0 35 998883.0 36 1479358.0 37 2345050.0 38 2451035.0 39 407410.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30906155406355 20.064747253739224 13.279974710591445 24.346216481605786 2 15.075331756713407 21.158363124354317 39.90025963549847 23.86604548343381 3 17.355977668798868 26.239642404091455 30.82236335425648 25.582016572853195 4 11.706970487598518 17.342324316896658 38.47592017589412 32.4747850196107 5 12.242947590277982 38.57805793353234 36.07124588723142 13.107748588958257 6 31.20411987213611 38.38904651553751 17.53018644106045 12.876647171265926 7 27.04481170156694 32.851949057025266 22.58035651965361 17.522882721754186 8 25.535641968278792 36.96187174891736 20.050574097350328 17.45191218545352 9 26.120448087394415 15.004969448149408 20.158194901205377 38.716387563250805 10-11 24.338096620076406 26.57880415245107 29.349567063785077 19.733532163687446 12-13 24.88693119885467 24.27701392704644 29.202604737116566 21.633450136982326 14-15 22.137431554758553 25.890127074043228 26.455217424998217 25.517223946199998 16-17 21.865480498576854 28.841202328637277 27.227741001948697 22.065576170837176 18-19 21.682867513078236 28.04474362042472 29.0082881298055 21.26410073669155 20-21 22.3814880738235 27.27437767751936 29.059685070322157 21.28444917833498 22-23 22.141630946898115 27.21241746871507 28.770212076827832 21.87573950755898 24-25 21.946358043990827 27.322147418837133 28.925416347028808 21.80607819014323 26-27 21.97905534174416 27.443575170079082 28.861105288461264 21.716264199715496 28-29 21.939997049704864 27.407524582111858 28.989313117849246 21.66316525033403 30-31 22.195449104519977 27.366753858123566 28.909732132275025 21.528064905081436 32-33 21.941885843058774 27.483015439602948 28.85706562129743 21.718033096040852 34-35 22.148674356194245 27.556463813314252 28.875693823735958 21.419168006755545 36-37 22.17029761582743 27.448290140520943 28.760656322165477 21.620755921486147 38-39 22.062067262896203 27.612363770429106 28.78308690155915 21.542482065115543 40-41 22.160828496893625 27.46615345057826 28.83579976122751 21.537218291300604 42-43 21.992537230202807 27.4483323576435 28.7549228235538 21.804207588599887 44-45 22.027438801043523 27.664316748382195 28.586060747579555 21.722183702994727 46-47 22.056296457505965 27.57259894890583 28.4043455932412 21.966759000347004 48-49 21.99696418406378 27.69272959041415 28.42764547565672 21.882660749865344 50-51 22.070944555512938 27.838571679123785 28.22600122377018 21.864482541593098 52-53 22.10431824583072 27.839512544150757 28.138460623385075 21.917708586633452 54-55 22.06924360868682 27.822987887933703 28.186535748734485 21.921232754644993 56-57 22.09341091809485 27.898185460725088 28.0112483879939 21.997155233186163 58-59 22.046251447246533 27.782895504219095 28.253777361012176 21.917075687522193 60-61 22.175780492805814 27.922159431658898 28.14987533248752 21.752184743047764 62-63 22.003330433532412 27.851547572095058 28.2937260243187 21.85139597005383 64-65 21.949329507252756 27.701081127842393 28.26198633656779 22.087603028337064 66-67 21.914119899416377 27.823385919937245 28.093451164929185 22.169043015717197 68-69 21.91626656239243 27.853472826369835 28.121349144177067 22.10891146706067 70-71 21.90565907600605 27.673335955452604 28.136745167401088 22.28425980114026 72-73 21.886122996512984 27.833096548632085 28.00284412810753 22.277936326747405 74-75 21.890733104616995 27.88893017468967 27.907591881462167 22.312744839231165 76-77 21.78663840570976 27.79418811181948 28.064098502357055 22.355074980113706 78-79 21.79169850720366 27.749876486708153 28.013133871613356 22.445291134474836 80-81 21.777956998300198 27.924281564673887 27.78699854730784 22.510762889718077 82-83 21.876633171148377 27.895665481758257 27.859133187332464 22.3685681597609 84-85 21.781040684218883 27.87484652271989 27.83139829120244 22.512714501858785 86-87 21.657213111713567 27.843550999854074 27.990817888084514 22.50841800034785 88-89 21.664646691225986 27.740661661630178 27.959354422643894 22.635337224499942 90-91 21.789484274968775 27.786115953377177 27.875975774331202 22.548423997322843 92-93 21.720863551073137 27.729553835445458 27.84867775468321 22.70090485879819 94-95 21.544867898268063 27.64573374796024 27.901525730531418 22.907872623240277 96-97 21.549694020079276 27.61711944281852 27.94967578774678 22.883510749355427 98-99 21.481226678324106 27.567250476138693 27.8954396321398 23.0560832133974 100 20.763975224288323 27.993333048136726 28.004242255796687 23.238449471778264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2407.0 1 1788.5 2 1681.5 3 2335.0 4 2062.0 5 1928.5 6 2972.0 7 5543.5 8 8838.0 9 10906.0 10 10067.0 11 8488.0 12 8685.5 13 9414.5 14 10092.5 15 10827.0 16 11668.5 17 12598.5 18 13747.5 19 15260.0 20 17287.5 21 20367.0 22 25107.5 23 31352.5 24 39272.5 25 49306.5 26 62869.5 27 79170.5 28 96930.0 29 117509.0 30 141025.5 31 165707.5 32 192780.0 33 222508.0 34 253024.0 35 280259.0 36 311910.0 37 342517.5 38 363655.0 39 383191.0 40 391492.5 41 393711.5 42 401293.0 43 412766.5 44 423458.0 45 435467.5 46 443250.0 47 444150.5 48 444886.5 49 440440.5 50 431788.0 51 415167.5 52 390785.5 53 368837.5 54 346251.0 55 317254.5 56 284531.0 57 250635.5 58 217779.0 59 185300.5 60 152443.5 61 122474.5 62 96975.5 63 74157.0 64 54479.5 65 39751.5 66 28756.5 67 20844.5 68 15370.0 69 11341.0 70 8428.5 71 6239.5 72 4671.0 73 3507.0 74 2625.5 75 1852.0 76 1226.5 77 797.0 78 523.0 79 350.0 80 226.0 81 178.0 82 137.5 83 94.0 84 65.5 85 55.0 86 46.5 87 30.0 88 20.5 89 15.0 90 12.0 91 8.5 92 10.0 93 8.5 94 5.5 95 6.0 96 4.0 97 5.0 98 6.5 99 6.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035686602357310002 2 0.06996498339610852 3 6.035234222192134E-4 4 6.560037198034928E-4 5 0.0015219286299441033 6 6.997373011237256E-4 7 0.0 8 0.0011545665468541474 9 2.624014879213971E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.084840173877722E-4 18-19 0.0011020862492698677 20-21 0.003542420086938861 22-23 4.3733581320232854E-6 24-25 1.3120074396069855E-5 26-27 1.6181425088486156E-4 28-29 9.402719983850064E-4 30-31 0.0014607016160957773 32-33 0.0030307371854921368 34-35 0.004141570151026051 36-37 0.0013426209465311485 38-39 0.0016137691507165921 40-41 0.0056022717671218285 42-43 0.0068180653278243014 44-45 0.005427337441840897 46-47 0.006778705104636093 48-49 0.008829810068555013 50-51 0.008545541789973498 52-53 0.003866048588708584 54-55 0.002226039289199852 56-57 0.002540921074705529 58-59 0.003293138673413534 60-61 0.007469695689495771 62-63 0.014147813557095329 64-65 0.00392727560255691 66-67 0.010321125191574953 68-69 0.012831432759356318 70-71 0.005541044753273503 72-73 0.007762710684341332 74-75 0.0031488178550567658 76-77 0.0031838047201129516 78-79 0.007819564340057634 80-81 0.005593525050857782 82-83 0.016443826576407552 84-85 0.017642126704581932 86-87 0.022688981988936804 88-89 0.021363854474933747 90-91 0.02481443404110012 92-93 0.035048092070034606 94-95 0.03879168663104654 96-97 0.022903276537405943 98-99 0.0184249578102141 100 0.018814186683964174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1432862E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.85797690014943 #Duplication Level Percentage of deduplicated Percentage of total 1 69.59574284192833 38.87477396013172 2 17.54808372777993 19.604009110164387 3 6.171492965943799 10.341813345933703 4 2.7227038763059768 6.083389209145862 5 1.350232942001729 3.7710640242076683 6 0.7569193333934775 2.536798958398162 7 0.45285387122006077 1.7706850754427377 8 0.31748913294814834 1.418744052341291 9 0.2107609450623636 1.0595412000672453 >10 0.8108311626536931 7.446162459948029 >50 0.034928515047255616 1.3514812103584168 >100 0.0244354047302326 2.7079933399361 >500 0.0022681251937281084 0.8439339354957242 >1k 0.0011832054527822966 1.1127599168088622 >5k 0.0 0.0 >10k+ 7.395033852742528E-5 1.0768502016202008 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30605 0.2676932512611453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28964 0.25333988987184486 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18639 0.16303004444556402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.746716264046571E-6 0.0 0.0 0.0 1.7493432528093142E-5 7 8.746716264046571E-6 0.0 0.0 0.0 1.7493432528093142E-5 8 8.746716264046571E-6 0.0 0.0 0.0 1.7493432528093142E-5 9 8.746716264046571E-6 0.0 0.0 4.373358132023285E-5 4.373358132023285E-5 10-11 8.746716264046571E-6 0.0 0.0 5.248029758427942E-5 4.373358132023285E-5 12-13 8.746716264046571E-6 0.0 0.0 5.685365571630271E-5 4.810693945225614E-5 14-15 1.3120074396069857E-5 4.3733581320232854E-6 0.0 6.1227013848326E-5 8.74671626404657E-5 16-17 2.624014879213971E-5 8.746716264046571E-6 0.0 7.872044637641914E-5 1.0496059516855884E-4 18-19 2.624014879213971E-5 3.4986865056186284E-5 0.0 7.872044637641914E-5 1.3994746022474513E-4 20-21 2.624014879213971E-5 3.4986865056186284E-5 0.0 9.621387890451227E-5 1.4432081835676842E-4 22-23 2.624014879213971E-5 3.4986865056186284E-5 0.0 1.749343252809314E-4 1.7056096714890813E-4 24-25 2.624014879213971E-5 3.4986865056186284E-5 0.0 3.1925514363769983E-4 2.0117447407307112E-4 26-27 2.624014879213971E-5 3.4986865056186284E-5 0.0 4.5045588759839837E-4 2.099211903371177E-4 28-29 2.624014879213971E-5 4.373358132023285E-5 0.0 0.0014169680347755444 2.099211903371177E-4 30-31 2.624014879213971E-5 4.373358132023285E-5 0.0 0.005453577590633037 2.1866790660116426E-4 32-33 2.624014879213971E-5 4.373358132023285E-5 0.0 0.014007866096870582 2.2741462286521083E-4 34-35 2.624014879213971E-5 4.373358132023285E-5 0.0 0.02794138510549677 2.44908055393304E-4 36-37 2.624014879213971E-5 4.373358132023285E-5 0.0 0.05126013066544492 2.492814135253273E-4 38-39 2.624014879213971E-5 4.373358132023285E-5 0.0 0.0853417105883024 2.5802812978937385E-4 40-41 2.624014879213971E-5 4.810693945225614E-5 0.0 0.12347302014141341 2.6240148792139713E-4 42-43 2.624014879213971E-5 5.248029758427942E-5 0.0 0.1625402283347774 2.75521562317467E-4 44-45 2.624014879213971E-5 5.248029758427942E-5 0.0 0.2114256255345337 2.8864163671353684E-4 46-47 2.624014879213971E-5 5.248029758427942E-5 0.0 0.2729194142289131 2.973883529775834E-4 48-49 2.624014879213971E-5 6.1227013848326E-5 0.0 0.3395344052958918 3.1488178550567655E-4 50-51 2.624014879213971E-5 6.997373011237257E-5 0.0 0.41070206217830674 3.1488178550567655E-4 52-53 2.624014879213971E-5 6.997373011237257E-5 0.0 0.48058832512803884 3.7610879935400253E-4 54-55 2.624014879213971E-5 6.997373011237257E-5 0.0 0.561372996542773 4.417091713343518E-4 56-57 2.624014879213971E-5 6.997373011237257E-5 0.0 0.6597473143645047 4.460825294663751E-4 58-59 2.624014879213971E-5 6.997373011237257E-5 0.0 0.7718321099301295 4.460825294663751E-4 60-61 4.373358132023285E-5 6.997373011237257E-5 0.0 0.8926636217598007 4.460825294663751E-4 62-63 4.373358132023285E-5 6.997373011237257E-5 0.0 1.0144922592435734 4.635759619944682E-4 64-65 4.373358132023285E-5 6.997373011237257E-5 0.0 1.1420062622989764 4.635759619944682E-4 66-67 4.373358132023285E-5 6.997373011237257E-5 0.0 1.2929702116582882 4.635759619944682E-4 68-69 4.373358132023285E-5 6.997373011237257E-5 0.0 1.4670867189685313 4.635759619944682E-4 70-71 4.373358132023285E-5 7.434708824439585E-5 0.0 1.6598993322931739 4.723226782585148E-4 72-73 4.373358132023285E-5 7.872044637641914E-5 0.0 1.8570634369591796 6.035234222192134E-4 74-75 4.373358132023285E-5 7.872044637641914E-5 0.0 2.055893791073486 6.035234222192134E-4 76-77 4.373358132023285E-5 7.872044637641914E-5 0.0 2.272965421956462 6.1227013848326E-4 78-79 4.373358132023285E-5 7.872044637641914E-5 0.0 2.5266420604044724 6.210168547473065E-4 80-81 4.373358132023285E-5 7.872044637641914E-5 0.0 2.803969819630465 6.428836454074229E-4 82-83 4.373358132023285E-5 7.872044637641914E-5 0.0 3.095134883986179 7.172307336518188E-4 84-85 4.373358132023285E-5 7.872044637641914E-5 0.0 3.3867635243038885 7.303508080478886E-4 86-87 4.373358132023285E-5 7.872044637641914E-5 0.0 3.6922119763187906 7.347241661799119E-4 88 4.373358132023285E-5 7.872044637641914E-5 0.0 3.940229489343963 7.434708824439585E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17895 0.0 47.04289 1 GTATCAA 28890 0.0 34.527554 1 TCAACGC 34410 0.0 28.077328 4 ATCAACG 34930 0.0 27.673162 3 CAACGCA 35140 0.0 27.467064 5 GTACATG 35015 0.0 27.211847 1 TACCTGG 9530 0.0 26.647964 2 AACGCAG 36520 0.0 26.518759 6 TATCAAC 37555 0.0 25.971928 2 TACATGG 36255 0.0 25.567125 2 ACATGGG 34925 0.0 25.039925 3 GTACCTG 10410 0.0 24.666903 1 ACGCAGA 41855 0.0 23.059885 7 CGCAGAG 42650 0.0 22.420712 8 CATGGGG 19165 0.0 22.067392 4 ACCTGGG 12170 0.0 20.310387 3 GAGTACT 29700 0.0 19.39775 12-13 GCAGAGT 48475 0.0 19.290232 9 ATGGGGG 11400 0.0 18.837572 5 AGAGTAC 44260 0.0 18.54228 10-11 >>END_MODULE