FastQCFastQC Report
Mon 6 Jun 2016
ERR1211504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13929219
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407500.2925505012161845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354520.25451534648137847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221710.1591690101218166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA229300.049.281021
GTATCAA407850.037.4385451
GTACATG582000.031.000951
ATCAACG476350.030.8390923
TCAACGC478700.030.7557544
CAACGCA483100.030.4049245
TACATGG589600.029.8842752
ACATGGG569900.029.363793
AACGCAG504350.029.1716946
TATCAAC527600.028.0216852
TACCTGG101150.027.5680032
ACGCAGA571600.025.4126747
CGCAGAG572200.025.2394228
GTACCTG115550.025.2227941
CATGGGG213400.025.1258434
CATGGGA325350.024.2798714
GCAGAGT642150.021.8847879
GAGTACT371550.020.35386812-13
ACCTGGG133550.020.1627393
ATGGGGG132400.020.0162685
Produced by FastQC (version 0.11.5)