FastQCFastQC Report
Mon 6 Jun 2016
ERR1211504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13929219
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460270.33043489372950485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401530.28826454663394985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272030.19529451005113782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248900.048.201441
GTATCAA430600.036.643161
ATCAACG521450.029.5857963
TCAACGC524700.029.4561734
CAACGCA530300.029.1804545
GTACATG592700.029.0971471
AACGCAG550000.028.1691366
ACATGGG578200.027.8766843
TACATGG608700.027.7753222
TATCAAC569500.027.1932752
TACCTGG100000.025.066332
CATGGGG204250.024.5025374
ACGCAGA628900.024.478037
CGCAGAG629550.024.2960078
CATGGGA334900.022.8575384
GTACCTG121150.021.7001531
GCAGAGT709900.021.181779
GAGTACT402550.020.4520212-13
AGAGTAC664250.019.34123610-11
ACCTGGG127350.019.077293
Produced by FastQC (version 0.11.5)