##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13929219 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16782563329645 33.0 31.0 34.0 30.0 34.0 2 32.37924839863599 34.0 31.0 34.0 31.0 34.0 3 32.393368788300336 34.0 31.0 34.0 30.0 34.0 4 36.0145148841439 37.0 35.0 37.0 35.0 37.0 5 35.952090422298625 37.0 35.0 37.0 35.0 37.0 6 35.968649426791266 37.0 35.0 37.0 35.0 37.0 7 35.897230777978294 37.0 35.0 37.0 35.0 37.0 8 35.89097787894641 37.0 35.0 37.0 35.0 37.0 9 37.549596571064036 39.0 37.0 39.0 35.0 39.0 10-11 37.51578825058318 39.0 37.0 39.0 35.0 39.0 12-13 37.44682655933545 39.0 37.0 39.0 34.0 39.0 14-15 38.69884693463431 40.0 38.0 41.0 34.0 41.0 16-17 38.6412658886331 40.0 38.0 41.0 34.0 41.0 18-19 38.65026887006371 40.0 38.0 41.0 34.0 41.0 20-21 38.60380197913466 40.0 38.0 41.0 34.0 41.0 22-23 38.504287175038314 40.0 38.0 41.0 34.0 41.0 24-25 38.41444800315079 40.0 38.0 41.0 34.0 41.0 26-27 38.25374229524283 40.0 38.0 41.0 34.0 41.0 28-29 38.29527707188752 40.0 38.0 41.0 34.0 41.0 30-31 38.22923855242709 40.0 38.0 41.0 34.0 41.0 32-33 38.061885845861134 40.0 37.5 41.0 33.0 41.0 34-35 37.96254563877558 40.0 37.0 41.0 33.0 41.0 36-37 37.91360786272368 40.0 37.0 41.0 33.0 41.0 38-39 37.83345476871316 40.0 37.0 41.0 33.0 41.0 40-41 37.68987848493157 40.0 37.0 41.0 33.0 41.0 42-43 37.56007634024564 40.0 37.0 41.0 32.0 41.0 44-45 37.39138350111374 40.0 37.0 41.0 31.5 41.0 46-47 37.15301708588256 40.0 36.5 41.0 31.0 41.0 48-49 36.9544151039624 40.0 36.0 41.0 30.0 41.0 50-51 36.78094898931519 39.0 36.0 41.0 30.0 41.0 52-53 36.61645559596701 39.0 36.0 41.0 30.0 41.0 54-55 36.45088647827276 39.0 35.0 40.5 29.0 41.0 56-57 36.28377272982786 39.0 35.0 40.0 28.5 41.0 58-59 36.0634758488613 39.0 35.0 40.0 28.0 41.0 60-61 35.79315459107937 38.0 34.5 40.0 28.0 41.0 62-63 35.505984937131075 38.0 34.0 40.0 27.0 41.0 64-65 35.22686720626619 38.0 34.0 40.0 26.5 41.0 66-67 34.91627850778999 37.0 34.0 40.0 26.0 41.0 68-69 34.53976981767607 37.0 33.5 39.5 26.0 41.0 70-71 34.16700060498725 36.0 33.0 39.0 26.0 40.0 72-73 33.76324831995247 36.0 32.5 39.0 25.0 40.0 74-75 33.34772247460536 35.0 32.0 38.0 25.0 40.0 76-77 31.743229789121703 34.0 30.0 36.0 22.5 39.0 78-79 32.552526563047074 35.0 31.0 37.0 24.0 39.0 80-81 32.486312333807085 35.0 32.0 36.5 24.0 39.0 82-83 32.17299850049022 35.0 31.0 36.0 23.5 37.5 84-85 31.84176830014662 34.5 31.0 35.5 23.0 37.0 86-87 31.527922635145586 34.0 31.0 35.0 22.0 37.0 88-89 31.228404837342282 34.0 31.0 35.0 20.5 36.0 90-91 30.931980752115393 34.0 30.0 35.0 20.0 36.0 92-93 30.712614145846942 34.0 30.0 35.0 20.0 36.0 94-95 30.487390140107642 34.0 30.0 35.0 19.0 35.0 96-97 30.282692626198212 34.0 29.0 35.0 19.0 35.0 98-99 30.128474970491887 34.0 29.0 35.0 18.5 35.0 100 28.288816049198452 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3029092934189137 1101 2 0.21406766489986495 1101 3 0.2807575239173019 1101 4 0.15385856335839065 1101 5 0.19109830790442572 1101 6 0.20146395832323805 1101 7 0.1977821546805032 1101 8 0.15788093611231346 1101 9 0.2489704570376361 1101 10-11 0.22508198471971497 1101 12-13 0.2499457425975251 1101 14-15 0.3126320789931256 1101 16-17 0.30397490372291713 1101 18-19 0.32693112441022265 1101 20-21 0.29171893042578745 1101 22-23 0.28938508633797255 1101 24-25 0.2536014718891124 1101 26-27 0.23174448057239516 1101 28-29 0.31728317614556545 1101 30-31 0.3422670964210326 1101 32-33 0.38618365082464834 1101 34-35 0.3692335126433548 1101 36-37 0.3817144748628749 1101 38-39 0.3545194741025739 1101 40-41 0.3549464506960831 1101 42-43 0.4102791857357886 1101 44-45 0.38356689753778994 1101 46-47 0.38282996185261453 1101 48-49 0.40857448290434206 1101 50-51 0.40735176738726153 1101 52-53 0.3591212833871893 1101 54-55 0.312167730615208 1101 56-57 0.29237196161825096 1101 58-59 0.3569853822494764 1101 60-61 0.32863618439682085 1101 62-63 0.33434947907488777 1101 64-65 0.3040518349955761 1101 66-67 0.24330062598771462 1101 68-69 0.2724463750570294 1101 70-71 0.2312470324847098 1101 72-73 0.16401906798481036 1101 74-75 0.1906918282683705 1101 76-77 0.20367872650023244 1101 78-79 0.2586169910079903 1101 80-81 0.2379498931008115 1101 82-83 0.23515430429337414 1101 84-85 0.21310595434725244 1101 86-87 0.13554936147210128 1101 88-89 0.1587284518088481 1101 90-91 0.08904910572180214 1101 92-93 0.08998049803398445 1101 94-95 0.0765754560627947 1101 96-97 0.08343987194082203 1101 98-99 0.019125444983654205 1101 100 0.03998206447800001 1102 1 0.3129303398870462 1102 2 0.25554678064631275 1102 3 0.30438834573027407 1102 4 0.21620790627528663 1102 5 0.22052387748446733 1102 6 0.1978656189304857 1102 7 0.17801986666022884 1102 8 0.1593258374581552 1102 9 0.2685294629727508 1102 10-11 0.23842770370931277 1102 12-13 0.28558696361511693 1102 14-15 0.3616132141522783 1102 16-17 0.3342920634382267 1102 18-19 0.2911343928784831 1102 20-21 0.3174060373621046 1102 22-23 0.32497617530690803 1102 24-25 0.3243492536985002 1102 26-27 0.2912805436284387 1102 28-29 0.3323709757969553 1102 30-31 0.38163619807721005 1102 32-33 0.4332027031869785 1102 34-35 0.3951616773357003 1102 36-37 0.42286066290346724 1102 38-39 0.41908551426100615 1102 40-41 0.41917405135310304 1102 42-43 0.3988258391790751 1102 44-45 0.41756368459052595 1102 46-47 0.4282884655230461 1102 48-49 0.4664846144468555 1102 50-51 0.4789540701985828 1102 52-53 0.4216087463606115 1102 54-55 0.4724652090855628 1102 56-57 0.4625778580528248 1102 58-59 0.5144743459728218 1102 60-61 0.5105733816575295 1102 62-63 0.5266115700675442 1102 64-65 0.4874538651861826 1102 66-67 0.4738271953954296 1102 68-69 0.4663573399605525 1102 70-71 0.4670925769274348 1102 72-73 0.468975378916042 1102 74-75 0.43755556842879173 1102 76-77 0.5009803456979647 1102 78-79 0.4642897944133466 1102 80-81 0.4851551437922055 1102 82-83 0.4468052180101836 1102 84-85 0.4131290629414899 1102 86-87 0.40856303078928846 1102 88-89 0.4120876242363103 1102 90-91 0.3282054046997054 1102 92-93 0.3410683972108224 1102 94-95 0.33128790878017966 1102 96-97 0.3650165873352442 1102 98-99 0.300418192510886 1102 100 0.4392331459460799 1103 1 0.31469251794761277 1103 2 0.28258840317901246 1103 3 0.3355848349456849 1103 4 0.16740593819169902 1103 5 0.1658538582263276 1103 6 0.19394608925460943 1103 7 0.1859294343399185 1103 8 0.21819039537800933 1103 9 0.27348762096300305 1103 10-11 0.24505175348148356 1103 12-13 0.24515962659980062 1103 14-15 0.38526206518461237 1103 16-17 0.3504134877912719 1103 18-19 0.362186951447228 1103 20-21 0.330007258340153 1103 22-23 0.3584994393441576 1103 24-25 0.37001318149656015 1103 26-27 0.34462901769745 1103 28-29 0.4266810553423639 1103 30-31 0.4746431105440294 1103 32-33 0.48199972331772045 1103 34-35 0.5147544263582162 1103 36-37 0.4835675159595212 1103 38-39 0.4724575352841569 1103 40-41 0.5270379211540899 1103 42-43 0.5467837439401606 1103 44-45 0.48822716618926165 1103 46-47 0.5738640856163997 1103 48-49 0.5529377424896325 1103 50-51 0.5398743699879773 1103 52-53 0.5488000267953836 1103 54-55 0.6157832594295698 1103 56-57 0.6382289247077608 1103 58-59 0.6413121128486097 1103 60-61 0.588949727721598 1103 62-63 0.6588775946312992 1103 64-65 0.7362721880210188 1103 66-67 0.743145306528568 1103 68-69 0.698940148369104 1103 70-71 0.6375046450904236 1103 72-73 0.586645428568751 1103 74-75 0.5976432028584782 1103 76-77 0.5863293954966373 1103 78-79 0.6178956548040304 1103 80-81 0.6656695842158555 1103 82-83 0.6422054641001509 1103 84-85 0.5770151126479242 1103 86-87 0.5609035626871552 1103 88-89 0.6144533509333066 1103 90-91 0.659750561131002 1103 92-93 0.6369880060539437 1103 94-95 0.6213255503313952 1103 96-97 0.6384137554451215 1103 98-99 0.6365703127263451 1103 100 0.7180452962097554 1104 1 0.22636482209858855 1104 2 0.20676345392569573 1104 3 0.2770792912272171 1104 4 0.09506068091525322 1104 5 0.14348388220201969 1104 6 0.16368185607296226 1104 7 0.2018443767187037 1104 8 0.20548909880815103 1104 9 0.2403703269829549 1104 10-11 0.2991482472965359 1104 12-13 0.31388575784262684 1104 14-15 0.3456081617647655 1104 16-17 0.2891694587075193 1104 18-19 0.3229592826261296 1104 20-21 0.2847124411987636 1104 22-23 0.33249934967643213 1104 24-25 0.33547079881932973 1104 26-27 0.3759693679355749 1104 28-29 0.45124903798481597 1104 30-31 0.3490627733374154 1104 32-33 0.35424556327736667 1104 34-35 0.4359628224577321 1104 36-37 0.3429514353404244 1104 38-39 0.4101268003027556 1104 40-41 0.4988144938571182 1104 42-43 0.5234181346410693 1104 44-45 0.4773203039674385 1104 46-47 0.47939829130337586 1104 48-49 0.5467372827449708 1104 50-51 0.5388493628015496 1104 52-53 0.5022384689169641 1104 54-55 0.5008497075366662 1104 56-57 0.5978275693459025 1104 58-59 0.5709109999380857 1104 60-61 0.5534141411908351 1104 62-63 0.5804364163433391 1104 64-65 0.6577042013049947 1104 66-67 0.5770313086596843 1104 68-69 0.5817081492395744 1104 70-71 0.44686335125035725 1104 72-73 0.59077466558373 1104 74-75 0.6274590789841099 1104 76-77 0.5237400253091273 1104 78-79 0.6334024666803941 1104 80-81 0.672422393743382 1104 82-83 0.515430250400648 1104 84-85 0.5725205483430322 1104 86-87 0.685173055344972 1104 88-89 0.6337196247306558 1104 90-91 0.5556802645752263 1104 92-93 0.5504287693771239 1104 94-95 0.5865333421675878 1104 96-97 0.5378099362869229 1104 98-99 0.5528407137914044 1104 100 0.7301843658042309 1105 1 0.2611759875415913 1105 2 0.18301830239020234 1105 3 0.22377394700679787 1105 4 0.1281490405441943 1105 5 0.09134945946546935 1105 6 0.15625923405686848 1105 7 0.18242461798252663 1105 8 0.15425634768391916 1105 9 0.1564687617321212 1105 10-11 0.2077381968815999 1105 12-13 0.21341494221961455 1105 14-15 0.30628277611751287 1105 16-17 0.29930284275923924 1105 18-19 0.28913694187027517 1105 20-21 0.27116353529680026 1105 22-23 0.31668544723862624 1105 24-25 0.3420362453123005 1105 26-27 0.33927558624105103 1105 28-29 0.40940683909945363 1105 30-31 0.4146663691731547 1105 32-33 0.47306917659071956 1105 34-35 0.49311569906604547 1105 36-37 0.46607658419287645 1105 38-39 0.4852561863476481 1105 40-41 0.4726151735489381 1105 42-43 0.4600656654350743 1105 44-45 0.45921586188862307 1105 46-47 0.493314954061951 1105 48-49 0.5443774574311959 1105 50-51 0.6096606741467738 1105 52-53 0.6149649302559013 1105 54-55 0.588511703186775 1105 56-57 0.6334334446483467 1105 58-59 0.6213026293807857 1105 60-61 0.7168644455982403 1105 62-63 0.6893982965284025 1105 64-65 0.6696589875567156 1105 66-67 0.6404584914482925 1105 68-69 0.6924213796587111 1105 70-71 0.7181094036911659 1105 72-73 0.7410370520229108 1105 74-75 0.7100308574084977 1105 76-77 0.6628810970181256 1105 78-79 0.7241115504318429 1105 80-81 0.7135488365073641 1105 82-83 0.7455193488383571 1105 84-85 0.7573899469571792 1105 86-87 0.7100811972301777 1105 88-89 0.6614057836473926 1105 90-91 0.7393183833480101 1105 92-93 0.7201797659924587 1105 94-95 0.849308394155468 1105 96-97 0.8410646285297965 1105 98-99 0.8706545162024106 1105 100 0.9814730665460125 1106 1 0.23526855469361863 1106 2 0.16820031541558222 1106 3 0.2126339441173286 1106 4 0.14642005100616018 1106 5 0.13795643467664576 1106 6 0.14125457966991917 1106 7 0.15105685478614106 1106 8 0.15051938329010994 1106 9 0.14396874231367462 1106 10-11 0.16833533323831062 1106 12-13 0.1916898812499852 1106 14-15 0.27586419112363814 1106 16-17 0.28718313154699615 1106 18-19 0.2718714662709871 1106 20-21 0.29411430615753176 1106 22-23 0.2740207369161709 1106 24-25 0.25832831279737434 1106 26-27 0.261324115453057 1106 28-29 0.2814075083024292 1106 30-31 0.3182180513374746 1106 32-33 0.37275283707748486 1106 34-35 0.36520329388634565 1106 36-37 0.3891571393499902 1106 38-39 0.3882472491997433 1106 40-41 0.39428299755528684 1106 42-43 0.41600904485658674 1106 44-45 0.45663969366167123 1106 46-47 0.5212939429526955 1106 48-49 0.5082077399964149 1106 50-51 0.5369180259291824 1106 52-53 0.5279525038280894 1106 54-55 0.45473342841172126 1106 56-57 0.5248087957123673 1106 58-59 0.6387524421782942 1106 60-61 0.6760430684209808 1106 62-63 0.680141663314977 1106 64-65 0.65427973830338 1106 66-67 0.6127759618921118 1106 68-69 0.6206886462433232 1106 70-71 0.701548489908923 1106 72-73 0.6451041128237236 1106 74-75 0.6116797471305162 1106 76-77 0.607879767069992 1106 78-79 0.6605247105146148 1106 80-81 0.6637006629033024 1106 82-83 0.6397579030423373 1106 84-85 0.6380861467576828 1106 86-87 0.5267674635867756 1106 88-89 0.6199153847337193 1106 90-91 0.6590796218412827 1106 92-93 0.6484050844075888 1106 94-95 0.7148694648388023 1106 96-97 0.7094657641898579 1106 98-99 0.7408008571016858 1106 100 0.7645430943135345 1107 1 0.20611710789958693 1107 2 0.14344676896804742 1107 3 0.2496364844340846 1107 4 0.14460022200231748 1107 5 0.1378513150950056 1107 6 0.1502695447736926 1107 7 0.14568400498532696 1107 8 0.14557180354166377 1107 9 0.20000905494482168 1107 10-11 0.18269241001176084 1107 12-13 0.17295326802423006 1107 14-15 0.22942050732910246 1107 16-17 0.2126075020537641 1107 18-19 0.19769290497875858 1107 20-21 0.22203869377760554 1107 22-23 0.27989292666992327 1107 24-25 0.2941657309453021 1107 26-27 0.27996592096129547 1107 28-29 0.27272524781544405 1107 30-31 0.2000942656143181 1107 32-33 0.22894321146572594 1107 34-35 0.2648570478173582 1107 36-37 0.32766329864836763 1107 38-39 0.3360219050423794 1107 40-41 0.3434902214417406 1107 42-43 0.33700674272195386 1107 44-45 0.32584226711679776 1107 46-47 0.31129355417348137 1107 48-49 0.3580525574114759 1107 50-51 0.340056676533564 1107 52-53 0.3796991537008978 1107 54-55 0.32988458278761357 1107 56-57 0.3239078020699111 1107 58-59 0.4007858654310823 1107 60-61 0.41428482713529746 1107 62-63 0.4510057369734497 1107 64-65 0.5167521529698504 1107 66-67 0.5110691070387503 1107 68-69 0.4740584756771966 1107 70-71 0.5743365555629296 1107 72-73 0.4766594087250766 1107 74-75 0.4964154193877022 1107 76-77 0.4601455600628732 1107 78-79 0.5560753122799085 1107 80-81 0.5659056466140697 1107 82-83 0.5507884382710841 1107 84-85 0.4450083958287756 1107 86-87 0.40466676924288336 1107 88-89 0.3792201658755907 1107 90-91 0.3335109949375159 1107 92-93 0.45062885326315794 1107 94-95 0.5066580221273931 1107 96-97 0.5221560839032797 1107 98-99 0.6593127594501773 1107 100 0.7787473317086189 1108 1 0.2520635257939077 1108 2 0.19136015596083666 1108 3 0.22311361954858455 1108 4 0.12665912866133056 1108 5 0.12949686832213558 1108 6 0.1277525386740379 1108 7 0.14128339866213935 1108 8 0.14595591658555662 1108 9 0.12337512490228875 1108 10-11 0.14340425670990697 1108 12-13 0.18882693730567723 1108 14-15 0.19534347709821986 1108 16-17 0.26592415975508743 1108 18-19 0.28542965620270166 1108 20-21 0.267695190475564 1108 22-23 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2316 58-59 -0.5322143065901841 2316 60-61 -0.3074481906159292 2316 62-63 -0.33836952102119966 2316 64-65 -0.4361537308053016 2316 66-67 -0.14880511260889762 2316 68-69 -0.1916206748021949 2316 70-71 -0.40981607418370203 2316 72-73 -0.40469338133390664 2316 74-75 -0.48823276305627417 2316 76-77 -0.43988804907055723 2316 78-79 -0.330430271502685 2316 80-81 -0.26901543383735316 2316 82-83 -0.22442889545373745 2316 84-85 -0.296609668965921 2316 86-87 -0.26118492854253716 2316 88-89 -0.26695110227602115 2316 90-91 -0.24198433337617686 2316 92-93 -0.13357959494343064 2316 94-95 -0.11360035090229204 2316 96-97 -0.034258240347003976 2316 98-99 -0.19032389451544063 2316 100 -0.3415324089761249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 24.0 11 55.0 12 132.0 13 412.0 14 1193.0 15 3015.0 16 7403.0 17 15887.0 18 28987.0 19 44652.0 20 62619.0 21 86631.0 22 110029.0 23 117492.0 24 120084.0 25 131220.0 26 154825.0 27 186701.0 28 228733.0 29 279040.0 30 337894.0 31 410657.0 32 504500.0 33 631884.0 34 825295.0 35 1114153.0 36 1649595.0 37 2776471.0 38 3428329.0 39 671138.0 40 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.44623313310499 21.246673430607338 13.616463025721451 23.69063041056622 2 14.135519197134016 22.283669207892586 41.37193870165064 22.208872893322752 3 16.630514534320238 28.44825580977953 31.260102949939334 23.661126705960903 4 10.70747085692748 18.5137616325505 40.87665233817631 29.902115172345717 5 11.130124988082425 39.94769763934374 36.809383697676104 12.11279367489774 6 28.308292268283996 40.54612926013991 19.069015128019572 12.07656334355652 7 25.534410795034525 33.70316024179101 24.118301248619897 16.644127714554564 8 24.477070519460835 37.53184794656531 21.339669527277362 16.651412006696496 9 26.22927799294906 15.105397555482854 21.393886359552404 37.27143809201568 10-11 23.033592192067623 27.71429970337892 30.475003659573446 18.777104444980008 12-13 24.027793661654684 25.623981502480504 30.072759283919652 20.27546555194516 14-15 21.397394929320875 26.989014962001818 27.747811991469156 23.86577811720815 16-17 20.483143980606382 29.892141778672432 28.53707402456681 21.087640216154373 18-19 20.25280386851689 29.02272508142869 30.61236943572426 20.112101614330157 20-21 21.23810806872807 28.28144755158577 30.564033542351112 19.916410837335047 22-23 20.769632553326662 28.465240171227123 30.088572449344518 20.676554826101697 24-25 20.599142859122253 28.513928153383798 30.434190640528175 20.45273834696577 26-27 20.57881898238579 28.695877755576625 30.355731639587834 20.369571622449747 28-29 20.48498505952588 28.450307609398862 30.625288716410765 20.43941861466449 30-31 20.841949639515096 28.464469421242676 30.465605036278227 20.227975902963998 32-33 20.572769330501835 28.607774684702363 30.403932978066607 20.415523006729195 34-35 20.80225102178193 28.731198408340592 30.4249390893907 20.04161148048678 36-37 20.829239067889485 28.460668668001766 30.2578785967585 20.45221366735025 38-39 20.62372646752491 28.838039424518126 30.34469217060996 20.193541937347003 40-41 20.765199317584408 28.557918722184876 30.53766343557302 20.139218524657693 42-43 20.627309901312223 28.48776902173318 30.25676756458354 20.628153512371053 44-45 20.660028144550026 28.787851973351092 30.109592584645895 20.442527297452987 46-47 20.720132149093846 28.739236545693338 29.8131363996408 20.72749490557202 48-49 20.610471930812725 28.87285643051997 29.846869560191543 20.66980207847576 50-51 20.81182200337973 28.874682443391038 29.572516311264007 20.74097924196522 52-53 20.798426182128647 28.973154771740155 29.487823196985012 20.740595849146185 54-55 20.82700238065837 28.963968491455187 29.51591724268644 20.693111885200004 56-57 20.84445784425241 29.026481256815195 29.31155782375903 20.817503075173367 58-59 20.729635760418592 28.893631824540627 29.638068588248416 20.738663826792365 60-61 20.971004010905826 29.253316870750357 29.287122507758657 20.48855661058516 62-63 20.691769000152078 29.004339572596834 29.65265827721883 20.65123315003226 64-65 20.62956510227028 28.803667065341887 29.58146425158366 20.98530358080417 66-67 20.659828886528928 28.95363253001192 29.32129149378897 21.065247089670187 68-69 20.67628249083281 29.02080720077488 29.35844045438521 20.944469854007103 70-71 20.713892761366193 28.760177256254256 29.450614382210187 21.07531560016937 72-73 20.684627519978793 28.912964204919493 29.22438404028448 21.178024234817233 74-75 20.617444121380963 28.984424150611034 29.125570481377196 21.272561246630815 76-77 20.454039798544038 28.861813016066495 29.41100960520579 21.27313758018368 78-79 20.509837528233305 28.74133328470278 29.28407972261629 21.464749464447628 80-81 20.428000904768258 29.100899645642002 29.005982261387203 21.46511718820253 82-83 20.710580532059396 28.910195342869184 29.068123269875308 21.311100855196113 84-85 20.470046129605567 28.935405160698235 28.97350453061896 21.621044179077234 86-87 20.30396721579756 28.938335414994803 29.26551536730766 21.492182001899977 88-89 20.526804836234056 28.7414517678105 29.16123832006391 21.570505075891536 90-91 20.604963008216266 28.837531109236487 29.07387423745007 21.483631645097176 92-93 20.536344478249415 28.767808056065373 29.01116650900482 21.684680956680385 94-95 20.27855117593587 28.60143534494739 29.064718936212707 22.055294542904033 96-97 20.429324117117407 28.563397281135877 29.061182377656465 21.946096224090255 98-99 20.184627147377626 28.434668785296864 29.11178985177906 22.26891421554645 100 19.55502320607784 28.832442323727015 29.18123718452645 22.4312972856687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2000.0 1 1483.0 2 1408.5 3 2019.5 4 1874.5 5 1884.5 6 2928.5 7 5609.0 8 9118.0 9 11452.0 10 11371.5 11 10505.0 12 11221.5 13 12421.5 14 13527.0 15 14828.0 16 16182.0 17 17732.0 18 19755.0 19 22519.5 20 26044.0 21 31132.5 22 38446.0 23 48298.5 24 60379.5 25 76588.0 26 99578.0 27 125997.0 28 153166.0 29 186481.5 30 226581.0 31 263884.0 32 307517.5 33 362325.5 34 419894.0 35 466685.5 36 516639.5 37 562608.0 38 584924.5 39 602138.0 40 593448.0 41 572939.5 42 560036.0 43 549878.5 44 546548.0 45 548787.5 46 535999.0 47 509359.0 48 491447.5 49 472771.0 50 452590.0 51 417506.0 52 372434.5 53 333556.5 54 297103.0 55 261021.0 56 224346.5 57 187978.0 58 154839.0 59 126040.0 60 98841.0 61 75369.0 62 56623.0 63 41511.0 64 29718.0 65 20687.5 66 14308.5 67 10165.5 68 7231.5 69 5065.5 70 3734.0 71 2713.0 72 1906.5 73 1369.0 74 1013.0 75 689.0 76 460.0 77 290.0 78 194.5 79 150.0 80 93.0 81 56.0 82 49.0 83 39.0 84 22.0 85 16.5 86 13.5 87 10.5 88 10.5 89 10.5 90 9.5 91 7.5 92 8.5 93 6.5 94 4.5 95 7.5 96 8.0 97 6.5 98 6.0 99 7.5 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032665147988555567 2 0.07239458292672403 3 8.543192550852994E-4 4 6.748404199833457E-4 5 0.0015148013682604888 6 7.107361870037365E-4 7 0.0 8 0.0010194397833790969 9 4.307492042446888E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.179153404078147E-4 18-19 0.0011486645446525034 20-21 0.0035859871253370343 22-23 3.589576702039073E-6 24-25 2.5127036914273513E-5 26-27 7.897068744485962E-5 28-29 7.86117297746557E-4 30-31 0.0014573681410278638 32-33 0.003126521307476033 34-35 0.0043864627298917476 36-37 0.0013209642263503792 38-39 0.001410703643901356 40-41 0.0054310295501851175 42-43 0.007175563827376108 44-45 0.0055100002376299775 46-47 0.007261713668225045 48-49 0.008787283766591651 50-51 0.008546782127555034 52-53 0.0038946907217123944 54-55 0.0021465668678193658 56-57 0.0023655310466437493 58-59 0.0031121630006678766 60-61 0.007670925412257499 62-63 0.014652652097723496 64-65 0.0042213422015979505 66-67 0.010783088412925376 68-69 0.013130671576058931 70-71 0.0057181956863482436 72-73 0.007724769062788085 74-75 0.0029578112024801965 76-77 0.0030008861229046655 78-79 0.007724769062788085 80-81 0.0056751207659237754 82-83 0.016486925792465464 84-85 0.017800710865411764 86-87 0.02328558406612747 88-89 0.022212300632217786 90-91 0.02506601411033885 92-93 0.03519938914019515 94-95 0.03918381927945852 96-97 0.02390658083558023 98-99 0.018741179961346 100 0.019347818423990606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3929219E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.25727876716842 #Duplication Level Percentage of deduplicated Percentage of total 1 67.0352036560173 31.679013063863177 2 17.795139499009593 16.818997360106923 3 6.777102278643407 9.608022348463914 4 3.242540377178746 6.129345380725418 5 1.7598513363869501 4.1582892596206 6 0.9928445178414944 2.815147809125426 7 0.6203628534082146 2.052166221021563 8 0.41069213754973855 1.552655426533782 9 0.2938905026257691 1.2499618868648292 >10 0.9649391377451207 7.281368801981331 >50 0.04619395262223663 1.5201971705239135 >100 0.04611548642530936 4.8492666670997675 >500 0.008672992884387365 2.8261730639725617 >1k 0.006177396922174132 5.311805322599382 >5k 1.8258315976008244E-4 0.5170702486312352 >10k+ 9.129157988004122E-5 1.6305199688662626 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46027 0.33043489372950485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40153 0.28826454663394985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27203 0.19529451005113782 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.179153404078147E-6 0.0 2 0.0 0.0 0.0 7.179153404078147E-6 0.0 3 0.0 0.0 0.0 7.179153404078147E-6 0.0 4 0.0 0.0 0.0 5.7433227232625176E-5 0.0 5 0.0 2.8716613616312588E-5 0.0 5.7433227232625176E-5 0.0 6 0.0 2.8716613616312588E-5 0.0 5.7433227232625176E-5 1.4358306808156294E-5 7 0.0 2.8716613616312588E-5 0.0 5.7433227232625176E-5 1.4358306808156294E-5 8 0.0 2.8716613616312588E-5 0.0 5.7433227232625176E-5 1.4358306808156294E-5 9 0.0 2.8716613616312588E-5 0.0 2.2255375552642255E-4 1.4358306808156294E-5 10-11 0.0 2.8716613616312588E-5 0.0 2.3691206233457883E-4 1.4358306808156294E-5 12-13 0.0 5.38436505305861E-5 0.0 2.44091215738657E-4 1.4358306808156294E-5 14-15 0.0 6.461238063670332E-5 0.0 2.8716613616312585E-4 1.4358306808156294E-5 16-17 0.0 6.461238063670332E-5 0.0 3.3383063328963386E-4 1.4358306808156294E-5 18-19 0.0 6.82019573387424E-5 0.0 3.5895767020390737E-4 1.4358306808156294E-5 20-21 0.0 7.897068744485962E-5 0.0 3.91263860522259E-4 1.4358306808156294E-5 22-23 0.0 7.897068744485962E-5 0.0 5.025407382854703E-4 2.8716613616312588E-5 24-25 0.0 8.256026414689869E-5 0.0 6.640716898772286E-4 2.8716613616312588E-5 26-27 0.0 8.614984084893776E-5 0.0 9.225212124240418E-4 2.8716613616312588E-5 28-29 0.0 8.614984084893776E-5 0.0 0.0018773486151664356 2.8716613616312588E-5 30-31 0.0 9.332899425301591E-5 0.0 0.006540208751115191 2.8716613616312588E-5 32-33 0.0 1.0050814765709405E-4 0.0 0.01658384436342052 2.8716613616312588E-5 34-35 0.0 1.1845603116728942E-4 0.0 0.03209440529293135 2.8716613616312588E-5 36-37 0.0 1.5435179818768018E-4 0.0 0.058650093734616424 2.8716613616312588E-5 38-39 0.0 1.7947883510195369E-4 0.0 0.10068762649219601 2.8716613616312588E-5 40-41 0.0 1.8665798850603182E-4 0.0 0.14749929626348757 2.8716613616312588E-5 42-43 0.0 1.9383714191010997E-4 0.0 0.19470940904870546 3.589576702039073E-5 44-45 0.0 1.9742671861214904E-4 0.0 0.25154676654879216 4.307492042446888E-5 46-47 0.0 2.010162953141881E-4 0.0 0.32113430049452163 4.307492042446888E-5 48-49 0.0 2.010162953141881E-4 0.0 0.39887376313058187 5.38436505305861E-5 50-51 0.0 2.010162953141881E-4 0.0 0.48543999487695616 5.7433227232625176E-5 52-53 0.0 2.046058720162272E-4 0.0 0.57123087805569 5.7433227232625176E-5 54-55 0.0 2.548599458447742E-4 0.0 0.6641255335277592 5.7433227232625176E-5 56-57 0.0 2.799869827590477E-4 0.0 0.7785504700586587 6.102280393466425E-5 58-59 0.0 3.0152444297128217E-4 0.0 0.9097243714812726 6.461238063670332E-5 60-61 0.0 3.0152444297128217E-4 0.0 1.054003817443031 6.461238063670332E-5 62-63 0.0 3.0152444297128217E-4 0.0 1.2018692505301267 6.82019573387424E-5 64-65 0.0 3.230619031835166E-4 0.0 1.3524986576777922 7.179153404078146E-5 66-67 0.0 3.3024105658759474E-4 0.0 1.5273541179875196 8.614984084893776E-5 68-69 0.0 4.2715962754264977E-4 0.0 1.7303087847208087 8.614984084893776E-5 70-71 0.0 5.133094683915874E-4 0.0 1.9602427099466238 8.973941755097684E-5 72-73 0.0 5.168990450936266E-4 0.0 2.1947677037743465 9.332899425301591E-5 74-75 0.0 5.958697325384862E-4 0.0 2.4264712903142667 9.332899425301591E-5 76-77 0.0 5.958697325384862E-4 0.0 2.675706369466946 9.332899425301591E-5 78-79 0.0 5.958697325384862E-4 0.0 2.9681025188849426 9.332899425301591E-5 80-81 0.0 5.994593092405252E-4 0.0 3.2962580314086525 9.332899425301591E-5 82-83 0.0 6.174071927507206E-4 0.0 3.6370775705371567 1.0768730106117221E-4 84-85 0.0 6.174071927507206E-4 0.0 3.972717350484618 1.0768730106117221E-4 86-87 0.0 6.35355076260916E-4 0.0 4.314545560666394 1.0768730106117221E-4 88 7.179153404078147E-6 6.891987267915021E-4 0.0 4.592310595446881 1.0768730106117221E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 24890 0.0 48.20144 1 GTATCAA 43060 0.0 36.64316 1 ATCAACG 52145 0.0 29.585796 3 TCAACGC 52470 0.0 29.456173 4 CAACGCA 53030 0.0 29.180454 5 GTACATG 59270 0.0 29.097147 1 AACGCAG 55000 0.0 28.169136 6 ACATGGG 57820 0.0 27.876684 3 TACATGG 60870 0.0 27.775322 2 TATCAAC 56950 0.0 27.193275 2 TACCTGG 10000 0.0 25.06633 2 CATGGGG 20425 0.0 24.502537 4 ACGCAGA 62890 0.0 24.47803 7 CGCAGAG 62955 0.0 24.296007 8 CATGGGA 33490 0.0 22.857538 4 GTACCTG 12115 0.0 21.700153 1 GCAGAGT 70990 0.0 21.18177 9 GAGTACT 40255 0.0 20.45202 12-13 AGAGTAC 66425 0.0 19.341236 10-11 ACCTGGG 12735 0.0 19.07729 3 >>END_MODULE