FastQCFastQC Report
Mon 6 Jun 2016
ERR1211503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11364934
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154980.13636682799917713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148410.13058588813626193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143550.038.3820721
GTATCAA226300.032.650071
TACCTGG107350.031.4537752
GTACCTG120750.029.0935971
GTACATG342000.028.571811
TCAACGC250250.028.4297644
CAACGCA252550.028.1319055
ATCAACG255300.027.868513
TACATGG346900.027.4247042
AACGCAG262250.027.2997746
ACATGGG346950.026.6019383
TATCAAC282300.025.4544012
CATGGGG211600.023.9622334
ACCTGGG140000.023.89963
ACGCAGA304050.023.2391957
CGCAGAG316400.022.1393668
ATGGGGG133550.019.7032955
GCAGAGT375200.018.295169
GTATAGG34950.018.0552431
GAGTACT196200.017.87677812-13
Produced by FastQC (version 0.11.5)