FastQCFastQC Report
Mon 6 Jun 2016
ERR1211503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11364934
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172280.15158908973866456No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166230.14626569762745653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163400.038.163161
TACCTGG108750.032.5611272
GTATCAA247150.030.4066811
GTACCTG119500.030.1055371
GTACATG363450.026.8747631
TCAACGC280750.026.0934374
TACATGG367550.025.9212972
CAACGCA285050.025.7161885
ATCAACG285450.025.565373
ACATGGG369500.024.9387043
AACGCAG297150.024.700436
ACCTGGG141350.024.3014533
TATCAAC309850.023.8732172
CATGGGG215500.022.9607494
ACGCAGA343250.021.2597547
ATGGGGG132850.020.6210165
CGCAGAG353450.020.3803428
GAGTACT209500.017.5178812-13
GCAGAGT411450.017.0960719
CCTGGGG136000.016.5846864
Produced by FastQC (version 0.11.5)