##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211503_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11364934 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13556761526288 33.0 31.0 34.0 30.0 34.0 2 32.34869828544539 34.0 31.0 34.0 31.0 34.0 3 32.369566334481135 34.0 31.0 34.0 30.0 34.0 4 35.997306627561585 37.0 35.0 37.0 35.0 37.0 5 35.920320082809106 37.0 35.0 37.0 35.0 37.0 6 35.931786933386505 37.0 35.0 37.0 35.0 37.0 7 35.85188695332503 37.0 35.0 37.0 35.0 37.0 8 35.845361882435924 37.0 35.0 37.0 35.0 37.0 9 37.50344735833926 39.0 37.0 39.0 35.0 39.0 10-11 37.46078186639711 39.0 37.0 39.0 34.5 39.0 12-13 37.379174045357416 39.0 37.0 39.0 34.0 39.0 14-15 38.64195973333413 40.0 38.0 41.0 34.0 41.0 16-17 38.56493680473639 40.0 38.0 41.0 34.0 41.0 18-19 38.528112305799574 40.0 38.0 41.0 34.0 41.0 20-21 38.455930848344565 40.0 38.0 41.0 34.0 41.0 22-23 38.35378392870561 40.0 38.0 41.0 34.0 41.0 24-25 38.24983735057326 40.0 38.0 41.0 33.5 41.0 26-27 38.082599555791525 40.0 38.0 41.0 33.0 41.0 28-29 38.13377816360394 40.0 38.0 41.0 33.0 41.0 30-31 38.089066861276976 40.0 38.0 41.0 33.0 41.0 32-33 37.93920914982876 40.0 37.0 41.0 33.0 41.0 34-35 37.84402843870453 40.0 37.0 41.0 33.0 41.0 36-37 37.80595030292301 40.0 37.0 41.0 33.0 41.0 38-39 37.7210982923438 40.0 37.0 41.0 32.5 41.0 40-41 37.57185915025991 40.0 37.0 41.0 32.0 41.0 42-43 37.437606500838456 40.0 37.0 41.0 31.5 41.0 44-45 37.27805801599904 40.0 36.0 41.0 31.0 41.0 46-47 37.05899136765774 39.5 36.0 41.0 31.0 41.0 48-49 36.87698899087316 39.0 36.0 41.0 30.0 41.0 50-51 36.693212956626056 39.0 35.5 41.0 30.0 41.0 52-53 36.496547846208344 39.0 35.0 40.0 29.5 41.0 54-55 36.30006865855974 39.0 35.0 40.0 29.0 41.0 56-57 36.09828389676526 38.5 35.0 40.0 28.5 41.0 58-59 35.835712156357445 38.0 34.0 40.0 28.0 41.0 60-61 35.518371466125544 38.0 34.0 40.0 28.0 41.0 62-63 35.19069041667994 37.5 34.0 40.0 27.0 41.0 64-65 34.86838304560325 37.0 33.5 40.0 26.0 41.0 66-67 34.52405315332231 36.5 33.0 39.0 26.0 41.0 68-69 34.11778225020929 36.0 33.0 39.0 26.0 40.0 70-71 33.73067085123415 35.5 32.0 38.5 26.0 40.0 72-73 33.32047920383876 35.0 32.0 38.0 25.0 40.0 74-75 32.911545416805765 35.0 31.5 37.0 24.5 39.0 76-77 31.353773413906318 33.5 29.5 35.5 22.5 38.0 78-79 32.192131384132985 34.0 31.0 36.0 24.0 38.5 80-81 32.16352972221396 34.5 31.0 36.0 24.0 37.5 82-83 31.885547773528646 34.0 31.0 35.0 24.0 37.0 84-85 31.594997560038625 34.0 31.0 35.0 23.0 37.0 86-87 31.3107630893413 34.0 31.0 35.0 22.5 36.0 88-89 31.040660904849954 34.0 30.0 35.0 20.5 36.0 90-91 30.76516058078296 34.0 30.0 35.0 20.0 35.5 92-93 30.566764312049678 34.0 29.5 35.0 20.0 35.0 94-95 30.35487957959105 34.0 29.0 35.0 19.0 35.0 96-97 30.157242576155745 34.0 29.0 35.0 19.0 35.0 98-99 30.000328818451564 34.0 29.0 35.0 18.5 35.0 100 28.100720074573243 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.336461732248857 1101 2 0.24492115250037472 1101 3 0.3094042436475277 1101 4 0.20106940541560903 1101 5 0.1964920955373728 1101 6 0.20272459585250857 1101 7 0.235616851033015 1101 8 0.22179354626583603 1101 9 0.2583215998469015 1101 10-11 0.26236748077905503 1101 12-13 0.26346449996592725 1101 14-15 0.3213392591122428 1101 16-17 0.35661422652685104 1101 18-19 0.34929237025133375 1101 20-21 0.34467166110351855 1101 22-23 0.32209724727685796 1101 24-25 0.2892035331129179 1101 26-27 0.24009074236785466 1101 28-29 0.2897403143773687 1101 30-31 0.32021617445245454 1101 32-33 0.42017880434499233 1101 34-35 0.3500594438006459 1101 36-37 0.32964694571953856 1101 38-39 0.3093309435334106 1101 40-41 0.31141820162945777 1101 42-43 0.32438937393326484 1101 44-45 0.3489390037293916 1101 46-47 0.4181256967543092 1101 48-49 0.3862045986917977 1101 50-51 0.3379170810662089 1101 52-53 0.34147413250857994 1101 54-55 0.2931677313064256 1101 56-57 0.2553883589180188 1101 58-59 0.2794426120669655 1101 60-61 0.2548628425066255 1101 62-63 0.26734516828292243 1101 64-65 0.20624992195595127 1101 66-67 0.1749469562236925 1101 68-69 0.1407858810971021 1101 70-71 0.17218199760024788 1101 72-73 0.11938987798610867 1101 74-75 0.16425220323062462 1101 76-77 0.2061629694641809 1101 78-79 0.28679279138887637 1101 80-81 0.19484420479795972 1101 82-83 0.16425002273429357 1101 84-85 0.15644361229925607 1101 86-87 0.11814599499933465 1101 88-89 0.07346463836819694 1101 90-91 0.06620883424023916 1101 92-93 -0.05642662050885505 1101 94-95 -0.05063214344952982 1101 96-97 -0.045675430352250856 1101 98-99 -0.17948642622772581 1101 100 -0.13084972930168703 1102 1 0.3084585963870978 1102 2 0.23408219694893972 1102 3 0.3294372469478546 1102 4 0.20182602295530927 1102 5 0.2026658363596141 1102 6 0.18031969458252917 1102 7 0.20549897633244996 1102 8 0.1892251404481584 1102 9 0.2680833459113501 1102 10-11 0.2507651875719432 1102 12-13 0.2654888331913483 1102 14-15 0.3502060755893055 1102 16-17 0.3218956490562377 1102 18-19 0.3015859322705907 1102 20-21 0.326295369811163 1102 22-23 0.3193536481224015 1102 24-25 0.3565948787346329 1102 26-27 0.3147057365652941 1102 28-29 0.36189686566810053 1102 30-31 0.4050265569594913 1102 32-33 0.3900595504192452 1102 34-35 0.3676555177986742 1102 36-37 0.4183109757000878 1102 38-39 0.38272048173535467 1102 40-41 0.39142653180326903 1102 42-43 0.34451665217953575 1102 44-45 0.3906070611628678 1102 46-47 0.371036485122886 1102 48-49 0.40188496066050305 1102 50-51 0.33852328227034434 1102 52-53 0.38002819669274857 1102 54-55 0.3661559611488201 1102 56-57 0.39210465172232745 1102 58-59 0.4475515702787476 1102 60-61 0.4277708753102587 1102 62-63 0.4180326139290358 1102 64-65 0.31559333575177817 1102 66-67 0.37447698668783147 1102 68-69 0.3914678069571096 1102 70-71 0.33057607743488404 1102 72-73 0.26645837238581294 1102 74-75 0.2943091207950843 1102 76-77 0.34024743229059595 1102 78-79 0.35426496306836697 1102 80-81 0.37227568644936326 1102 82-83 0.34288149088197883 1102 84-85 0.3017930842983887 1102 86-87 0.19634038149516542 1102 88-89 0.18799025704301897 1102 90-91 0.1484459634436277 1102 92-93 0.07521584623825817 1102 94-95 0.11374164215424543 1102 96-97 0.1811176171986979 1102 98-99 0.1908456179051683 1102 100 0.23079356844302268 1103 1 0.28089987478524847 1103 2 0.23799035008109115 1103 3 0.3168342434823188 1103 4 0.15281623433281055 1103 5 0.16351965554369485 1103 6 0.1490226514355797 1103 7 0.1394800881416245 1103 8 0.12951677249323268 1103 9 0.18603138312027312 1103 10-11 0.16291655656045378 1103 12-13 0.19449558186897065 1103 14-15 0.27794790320417206 1103 16-17 0.2703335227161361 1103 18-19 0.2712911589491469 1103 20-21 0.3061448925398196 1103 22-23 0.30209760369867666 1103 24-25 0.28568644187428305 1103 26-27 0.330045602402393 1103 28-29 0.35853640927103925 1103 30-31 0.36293477089054704 1103 32-33 0.3646869545089899 1103 34-35 0.3829075292288735 1103 36-37 0.3730033332786675 1103 38-39 0.35764042985362465 1103 40-41 0.45172475039557014 1103 42-43 0.4101043748555 1103 44-45 0.39137282327704526 1103 46-47 0.39189719484868135 1103 48-49 0.34729008398092986 1103 50-51 0.38320358140092026 1103 52-53 0.3886429951444086 1103 54-55 0.38400580813825513 1103 56-57 0.4291693822818914 1103 58-59 0.40891762017437117 1103 60-61 0.3606771639428956 1103 62-63 0.3617327335002045 1103 64-65 0.4011515709624476 1103 66-67 0.39599594581070363 1103 68-69 0.3535461159371067 1103 70-71 0.40474647647830864 1103 72-73 0.3824944869309377 1103 74-75 0.4268474747662694 1103 76-77 0.4002822903251726 1103 78-79 0.4365344845810384 1103 80-81 0.3806129379338685 1103 82-83 0.3720465193561324 1103 84-85 0.32507602210111486 1103 86-87 0.3169469518293546 1103 88-89 0.3101619936621951 1103 90-91 0.30771850463488093 1103 92-93 0.26641561114918844 1103 94-95 0.3422090032794287 1103 96-97 0.46002891160722825 1103 98-99 0.35598302341355037 1103 100 0.37327619009148094 1104 1 0.24057285580462207 1104 2 0.18499486748861216 1104 3 0.2392991889634999 1104 4 0.08634898475465747 1104 5 0.14357043333294683 1104 6 0.18146659081529037 1104 7 0.1959060455471331 1104 8 0.18892271618686607 1104 9 0.21875926488503694 1104 10-11 0.25082662164492575 1104 12-13 0.2772921795692511 1104 14-15 0.3376681557782817 1104 16-17 0.28994667860894907 1104 18-19 0.32380500387966293 1104 20-21 0.20292416568488392 1104 22-23 0.3131239286326988 1104 24-25 0.3290660762867219 1104 26-27 0.38651836177766086 1104 28-29 0.3882027534893595 1104 30-31 0.30233608134570034 1104 32-33 0.3205732022432386 1104 34-35 0.3761644454329911 1104 36-37 0.2739158934246788 1104 38-39 0.3788459484163198 1104 40-41 0.4489891048257917 1104 42-43 0.5055328085391437 1104 44-45 0.444005473207703 1104 46-47 0.4277377989227986 1104 48-49 0.4215021986473175 1104 50-51 0.4170922772382113 1104 52-53 0.41330544180738116 1104 54-55 0.4530294783518585 1104 56-57 0.5433099948085314 1104 58-59 0.5437877486215186 1104 60-61 0.46909416166697326 1104 62-63 0.4569820987778357 1104 64-65 0.5067122397766823 1104 66-67 0.4554192641486239 1104 68-69 0.4790057613691374 1104 70-71 0.380032218939121 1104 72-73 0.48632739607250386 1104 74-75 0.5254983571164473 1104 76-77 0.4555541643762169 1104 78-79 0.5624353833666902 1104 80-81 0.6405329206531363 1104 82-83 0.5049575392634011 1104 84-85 0.500768894740272 1104 86-87 0.556267650196137 1104 88-89 0.5842466942704654 1104 90-91 0.465551964756159 1104 92-93 0.45902653494806955 1104 94-95 0.49176947173740615 1104 96-97 0.45394606429434603 1104 98-99 0.5039944875482121 1104 100 0.6054897197739493 1105 1 0.23904349342247144 1105 2 0.1464004076008294 1105 3 0.222126702969085 1105 4 0.1356980068250806 1105 5 0.10914511697745155 1105 6 0.11499220470353322 1105 7 0.16022370081046944 1105 8 0.1320157566515121 1105 9 0.14972325110632312 1105 10-11 0.207210477218446 1105 12-13 0.24010175275319057 1105 14-15 0.278296434359099 1105 16-17 0.30448558796344827 1105 18-19 0.3180586846267772 1105 20-21 0.2655326840051089 1105 22-23 0.3419879117256883 1105 24-25 0.32803377508578535 1105 26-27 0.3072522779362856 1105 28-29 0.3433515882859268 1105 30-31 0.36379657671739096 1105 32-33 0.35479896862057103 1105 34-35 0.3931743840347366 1105 36-37 0.42114893867119463 1105 38-39 0.40816968481798455 1105 40-41 0.38953523425478664 1105 42-43 0.3817565471640165 1105 44-45 0.4437303018330212 1105 46-47 0.4503396892817193 1105 48-49 0.48508522014789435 1105 50-51 0.5154033071980422 1105 52-53 0.5265690630238851 1105 54-55 0.4669631741004139 1105 56-57 0.4418681115308303 1105 58-59 0.5059496463633621 1105 60-61 0.49500562397071945 1105 62-63 0.4606120694749549 1105 64-65 0.4701409081480037 1105 66-67 0.44743186862458373 1105 68-69 0.5322586684470494 1105 70-71 0.6035931847078615 1105 72-73 0.5174892567848275 1105 74-75 0.520706077832962 1105 76-77 0.49770300050168714 1105 78-79 0.6185749859598175 1105 80-81 0.5890651167096266 1105 82-83 0.5611929391350188 1105 84-85 0.5744945933977554 1105 86-87 0.5238649233578947 1105 88-89 0.6017691200281554 1105 90-91 0.6715894901367356 1105 92-93 0.6018908866841386 1105 94-95 0.6023785323192463 1105 96-97 0.6730429702676268 1105 98-99 0.6045704151476343 1105 100 0.7848257807931418 1106 1 0.22793404281566865 1106 2 0.1869005710090903 1106 3 0.19537474694785573 1106 4 0.11738875791771619 1106 5 0.11166842094608143 1106 6 0.1319873402592222 1106 7 0.1597629565956069 1106 8 0.14386388574891384 1106 9 0.15170999064819313 1106 10-11 0.1627673609741933 1106 12-13 0.20439478948834022 1106 14-15 0.2338330140291589 1106 16-17 0.26184366362391387 1106 18-19 0.26914202954502997 1106 20-21 0.28465239518710916 1106 22-23 0.26194234643067205 1106 24-25 0.28130704978726584 1106 26-27 0.29971542876454293 1106 28-29 0.36386790655155465 1106 30-31 0.2998744188015934 1106 32-33 0.34520610822000464 1106 34-35 0.3698098293588288 1106 36-37 0.3557824865083532 1106 38-39 0.388171315851892 1106 40-41 0.3498754862205615 1106 42-43 0.31464705631488243 1106 44-45 0.35382487131324325 1106 46-47 0.3646719409499468 1106 48-49 0.38067842870561464 1106 50-51 0.432638942514707 1106 52-53 0.49631021032057276 1106 54-55 0.3606587219570869 1106 56-57 0.4356079411960181 1106 58-59 0.5584764410494287 1106 60-61 0.5443430675095087 1106 62-63 0.4803237910906901 1106 64-65 0.5170360049247122 1106 66-67 0.5282131826464749 1106 68-69 0.45623490047213977 1106 70-71 0.5501739980175842 1106 72-73 0.5751660791527371 1106 74-75 0.5698436602687735 1106 76-77 0.48269691537330317 1106 78-79 0.6064921129743794 1106 80-81 0.5793086986674041 1106 82-83 0.5738627526301059 1106 84-85 0.51485346963673 1106 86-87 0.46290552482222935 1106 88-89 0.5575661499001612 1106 90-91 0.5467853666391171 1106 92-93 0.5999838208623167 1106 94-95 0.6324443559700335 1106 96-97 0.6546596754693752 1106 98-99 0.6200549694089297 1106 100 0.7507113316009146 1107 1 0.21319413108033558 1107 2 0.21906066881984998 1107 3 0.24036150480013418 1107 4 0.13787618399667423 1107 5 0.13719050669449473 1107 6 0.1467853240749193 1107 7 0.18954637649652284 1107 8 0.16395614631326083 1107 9 0.2096300555616395 1107 10-11 0.22432728169265204 1107 12-13 0.21081796239015915 1107 14-15 0.25812687527788825 1107 16-17 0.24253461429262302 1107 18-19 0.23549831858610304 1107 20-21 0.24482837990356643 1107 22-23 0.34171781125208867 1107 24-25 0.36082748087913785 1107 26-27 0.3454987379062757 1107 28-29 0.33341315443183106 1107 30-31 0.30172328330579035 1107 32-33 0.2559215972493334 1107 34-35 0.31135256098626485 1107 36-37 0.39157589860105446 1107 38-39 0.36978020926361665 1107 40-41 0.35920065988607064 1107 42-43 0.3850579178132705 1107 44-45 0.3954880336653943 1107 46-47 0.33953316152839363 1107 48-49 0.42369667153869983 1107 50-51 0.35046691514433803 1107 52-53 0.3765072301030159 1107 54-55 0.3771368705775586 1107 56-57 0.43499951223854794 1107 58-59 0.5108346968309334 1107 60-61 0.4616045283313497 1107 62-63 0.4006453384638107 1107 64-65 0.49787366255767296 1107 66-67 0.5529957183097167 1107 68-69 0.4895084615818419 1107 70-71 0.5385413556339032 1107 72-73 0.5671160771945551 1107 74-75 0.5691374817420964 1107 76-77 0.5246198539062412 1107 78-79 0.6508523379440234 1107 80-81 0.6376695178202993 1107 82-83 0.5974642527133227 1107 84-85 0.5060296587375426 1107 86-87 0.495622677075108 1107 88-89 0.4678318128397727 1107 90-91 0.34426021023001496 1107 92-93 0.41714848978414665 1107 94-95 0.5806564059573063 1107 96-97 0.6515688333568974 1107 98-99 0.7274069682144422 1107 100 0.8684091315930829 1108 1 0.22699143577893466 1108 2 0.1852276456598574 1108 3 0.22352306928327437 1108 4 0.15559582021995055 1108 5 0.1316577245078605 1108 6 0.1266494980214361 1108 7 0.15905559884497222 1108 8 0.1521446352954925 1108 9 0.15540501312639066 1108 10-11 0.13600781196111456 1108 12-13 0.17735025335439758 1108 14-15 0.21038230627949162 1108 16-17 0.2461482172676952 1108 18-19 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82-83 -0.05467951416818906 2316 84-85 -0.04072160480655995 2316 86-87 -0.07511016356246003 2316 88-89 -0.07351648755021145 2316 90-91 0.05336894541954251 2316 92-93 0.19175987331786715 2316 94-95 0.20547245157032634 2316 96-97 0.23318734356017146 2316 98-99 0.07008725514170777 2316 100 -0.10695271336714995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 7.0 10 16.0 11 56.0 12 141.0 13 343.0 14 966.0 15 2482.0 16 5589.0 17 10807.0 18 19182.0 19 29599.0 20 40995.0 21 56815.0 22 73424.0 23 87424.0 24 99699.0 25 116233.0 26 140478.0 27 171601.0 28 208511.0 29 252861.0 30 305269.0 31 368648.0 32 452360.0 33 567594.0 34 743646.0 35 1009886.0 36 1506622.0 37 2374089.0 38 2361502.0 39 358047.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11199057137875 19.212214900621635 12.574466239126492 25.10132828887313 2 15.395397646485348 20.64843516387995 39.42203080187219 24.534136387762512 3 17.740547787159922 25.551173037713117 30.279224049728054 26.429055125398904 4 12.002668927670406 16.69631066416633 37.58557075562013 33.71544965254314 5 12.655280582502915 38.136668206515765 35.626379814778154 13.581671396203172 6 32.269596653689256 37.26197052426626 17.44053651628694 13.027896305757547 7 28.177303977304224 32.14811454250416 21.946568277475258 17.728013202716355 8 26.19551722287091 36.583283691881 19.657047223759616 17.56415186148847 9 26.613406329823412 14.716949422477747 19.443357768151674 39.22628647954717 10-11 24.962322702445963 26.019812345588633 28.99276845778427 20.025096494181135 12-13 25.300274511053033 23.565451413972134 28.7679849262653 22.36628914870953 14-15 22.545665465369176 25.277700688802945 25.781984303648397 26.394649542179476 16-17 22.635082890781497 28.193895316569765 26.56021547815198 22.610806314496752 18-19 22.430579261155493 27.61921698055142 28.009680933171495 21.94052282512159 20-21 23.11749399479987 26.871588259448227 27.928623152810033 22.08229459294187 22-23 22.88719406553527 26.770313844321493 27.67654875954405 22.66594333059919 24-25 22.701089513730064 26.843736203448636 27.831577530266898 22.623596752554402 26-27 22.739356565472498 27.00633889363204 27.74752776461104 22.506776776284422 28-29 22.68996219383793 27.004234297191314 27.872770289069404 22.433033219901354 30-31 22.96425669908069 26.9331715814479 27.809078807056675 22.293492912414735 32-33 22.715735465529 27.035937729400096 27.762582635729732 22.485744169341174 34-35 22.891502071947478 27.126114299275393 27.78209534328562 22.20028828549151 36-37 22.955561962288844 27.004998205200547 27.697000192568627 22.34243963994198 38-39 22.836937669127504 27.155514878160886 27.698319933946703 22.309227518764914 40-41 22.935285753210902 26.97441164496807 27.7689003110225 22.321402290798535 42-43 22.738097469429434 26.969552495807637 27.726566877127368 22.565783157635565 44-45 22.73944877226053 27.1747301493329 27.63684229468299 22.44897878372358 46-47 22.749021330838705 27.043246586614387 27.49786455783742 22.709867524709487 48-49 22.7223358446323 27.107064810855313 27.544339615958652 22.626259728553734 50-51 22.752405286694053 27.255281317389784 27.414979646998756 22.577333748917408 52-53 22.796537503914067 27.303821417655506 27.299632921422006 22.60000815700842 54-55 22.704357551141083 27.22338763040448 27.42912665953343 22.643128158921005 56-57 22.784972076964046 27.26187062298811 27.235662116966225 22.717495183081617 58-59 22.695179120307795 27.190269534982654 27.47132762661308 22.643223718096472 60-61 22.88420247693166 27.300093452516087 27.37273034461275 22.442973725939495 62-63 22.687008084061603 27.321019963812404 27.416934125258052 22.57503782686794 64-65 22.59892372270881 27.157604320596057 27.43496924906312 22.808502707632012 66-67 22.621637523339626 27.186224724610476 27.32497767896821 22.86716007308169 68-69 22.569882862977103 27.21847795439481 27.366703263552843 22.844935919075237 70-71 22.517223185665465 27.047837039607785 27.409895275850793 23.025044498875957 72-73 22.48791070356192 27.202915607389826 27.25564754177449 23.053526147273757 74-75 22.47655463086242 27.271720959036784 27.20263368451415 23.049090725586648 76-77 22.406177553042177 27.144555341297945 27.32365591307964 23.125611192580234 78-79 22.39435509840726 27.064117997985658 27.33954419052863 23.201982713078454 80-81 22.35269168428037 27.28514659115508 27.12346866403972 23.238693060524838 82-83 22.424319148339798 27.22971897591219 27.176872153680492 23.16908972206752 84-85 22.378272880998615 27.212194979532306 27.117844111261856 23.291688028207222 86-87 22.24891526186435 27.17446095552183 27.268882822142288 23.30774096047153 88-89 22.218938500612524 27.04803494564309 27.265655332238524 23.46737122150586 90-91 22.351668418400227 27.073674375409144 27.196349998653414 23.378307207537212 92-93 22.20771982501122 27.028480083147926 27.22015847784654 23.543641613994314 94-95 22.082386907661203 26.915679331726977 27.2432527546197 23.75868100599212 96-97 22.064217864125705 26.8779635626652 27.30683456028104 23.750984012928043 98-99 21.999751910057146 26.816885904456157 27.2239945481817 23.959367637305 100 21.368995967536115 27.216447031448286 27.290909442935618 24.123647558079977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 382.0 1 327.0 2 456.5 3 726.0 4 709.0 5 708.0 6 1112.0 7 1972.0 8 3090.0 9 4197.5 10 4652.0 11 4659.0 12 5165.5 13 5826.0 14 6272.5 15 6853.5 16 7507.0 17 8175.0 18 8991.5 19 10008.0 20 11246.0 21 13148.0 22 15923.5 23 20259.5 24 26024.0 25 33174.0 26 43195.5 27 55752.0 28 70848.5 29 89484.0 30 110118.5 31 132400.5 32 157795.0 33 185024.5 34 214055.5 35 241683.0 36 272163.0 37 303145.5 38 327861.5 39 349936.5 40 364534.5 41 376035.0 42 389587.5 43 404695.0 44 420534.5 45 436159.0 46 448266.5 47 455117.0 48 463169.0 49 467901.5 50 465437.0 51 452984.0 52 432029.5 53 412290.0 54 390655.5 55 361990.5 56 327000.0 57 290038.5 58 254169.0 59 217010.0 60 179416.5 61 145448.5 62 116289.5 63 89498.5 64 65808.0 65 48236.0 66 35368.5 67 26179.0 68 19580.0 69 14416.5 70 10918.0 71 8164.0 72 6013.5 73 4514.5 74 3425.5 75 2411.5 76 1602.5 77 1030.5 78 645.0 79 428.0 80 300.0 81 208.5 82 150.5 83 109.0 84 86.5 85 67.5 86 49.0 87 31.5 88 17.5 89 13.0 90 8.5 91 7.5 92 7.5 93 7.5 94 5.0 95 2.0 96 4.5 97 5.5 98 5.0 99 6.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003414010147353253 2 0.0700575999825428 3 7.215176084612547E-4 4 6.159296657596076E-4 5 0.001619015121425254 6 5.279397135082351E-4 7 0.0 8 9.15095503414274E-4 9 3.5195980900549E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.115301681470389E-4 18-19 0.0010602789246290387 20-21 0.0035943895494685674 22-23 0.0 24-25 1.3198492837705875E-5 26-27 1.4958291882733325E-4 28-29 0.0010558794270164702 30-31 0.001786196030702862 32-33 0.003286424716588763 34-35 0.004443492588694312 36-37 0.0013594447622837054 38-39 0.0015178266763361758 40-41 0.005662153427375821 42-43 0.007188779098937135 44-45 0.00521780416850639 46-47 0.006836819289931644 48-49 0.009040967593828525 50-51 0.00886938718693835 52-53 0.004166324239102489 54-55 0.002586904596190352 56-57 0.0026660955532165874 58-59 0.003339218687939587 60-61 0.007690321826769957 62-63 0.014505143628638759 64-65 0.004254314191353861 66-67 0.01066438221286635 68-69 0.013110502885454504 70-71 0.005296995125532626 72-73 0.007501143429429507 74-75 0.002842075457719332 76-77 0.003251228735688214 78-79 0.00800268615726233 80-81 0.005930522781742507 82-83 0.016757686406273897 84-85 0.017848761814190914 86-87 0.0231369579444984 88-89 0.021887500622528915 90-91 0.02512993036299199 92-93 0.03499360401037085 94-95 0.03895755135929518 96-97 0.023400927801252518 98-99 0.0186494703796784 100 0.01895303571494564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1364934E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.15628628508988 #Duplication Level Percentage of deduplicated Percentage of total 1 70.01735672997421 40.019320861836704 2 17.60247296922252 20.121839687088773 3 6.050310119645817 10.374397719361582 4 2.524308165425646 5.771203206994331 5 1.2441643444298882 3.5555906727967925 6 0.7296405767220153 2.5022127413004958 7 0.4339587704436799 1.7362430203583201 8 0.29707321161537503 1.3583681224575572 9 0.2031486037224545 1.0450097777499194 >10 0.8364240213771067 8.011107900763236 >50 0.0372704960926381 1.4499781939856153 >100 0.021691282468777974 2.34217355822758 >500 0.0014696941664623796 0.5778742257166453 >1k 6.399132313819678E-4 0.5376263282200865 >5k 0.0 0.0 >10k+ 7.110146200597778E-5 0.5970539831424012 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17228 0.15158908973866456 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16623 0.14626569762745653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.75979904502745E-5 2 0.0 0.0 0.0 0.0 1.75979904502745E-5 3 0.0 0.0 0.0 0.0 1.75979904502745E-5 4 0.0 0.0 0.0 0.0 1.75979904502745E-5 5 0.0 0.0 0.0 0.0 2.6396985675411753E-5 6 3.5195980900549E-5 0.0 0.0 0.0 3.5195980900549E-5 7 3.5195980900549E-5 0.0 0.0 0.0 3.5195980900549E-5 8 3.5195980900549E-5 0.0 0.0 0.0 3.5195980900549E-5 9 3.5195980900549E-5 0.0 0.0 3.5195980900549E-5 7.0391961801098E-5 10-11 3.5195980900549E-5 0.0 0.0 4.3994976125686255E-5 7.0391961801098E-5 12-13 3.5195980900549E-5 4.399497612568625E-6 0.0 5.27939713508235E-5 7.0391961801098E-5 14-15 4.3994976125686255E-5 8.79899522513725E-6 0.0 6.599246418852937E-5 1.231859331519215E-4 16-17 1.3198492837705877E-4 8.79899522513725E-6 0.0 7.0391961801098E-5 1.6718090927760778E-4 18-19 1.3198492837705877E-4 8.79899522513725E-6 0.0 7.919095702623526E-5 1.9357789495301952E-4 20-21 1.3198492837705877E-4 8.79899522513725E-6 0.0 9.238944986394114E-5 1.9357789495301952E-4 22-23 1.363844259896274E-4 8.79899522513725E-6 0.0 1.1878643553935289E-4 2.6836935436668613E-4 24-25 1.40783923602196E-4 8.79899522513725E-6 0.0 1.9797739256558817E-4 3.5195980900549004E-4 26-27 1.40783923602196E-4 8.79899522513725E-6 0.0 3.123643304923724E-4 3.607588042306273E-4 28-29 1.40783923602196E-4 8.79899522513725E-6 0.0 0.0011790653601683916 3.607588042306273E-4 30-31 1.40783923602196E-4 8.79899522513725E-6 0.0 0.005050623259228782 3.7395729706833316E-4 32-33 1.40783923602196E-4 8.79899522513725E-6 0.0 0.013383271737433759 3.8715578990603905E-4 34-35 1.40783923602196E-4 8.79899522513725E-6 0.0 0.027360475652564283 3.959547851311763E-4 36-37 1.40783923602196E-4 1.3198492837705875E-5 0.0 0.050941782855932115 3.959547851311763E-4 38-39 1.4958291882733327E-4 1.75979904502745E-5 0.0 0.08791956028957142 4.0035428274374493E-4 40-41 1.5838191405247051E-4 1.75979904502745E-5 0.0 0.13105223488319423 4.047537803563136E-4 42-43 1.5838191405247051E-4 2.6396985675411753E-5 0.0 0.17342379638984265 4.047537803563136E-4 44-45 1.6278141166503916E-4 2.6396985675411753E-5 0.0 0.22828993111618598 4.0915327796888217E-4 46-47 1.6718090927760778E-4 3.5195980900549E-5 0.0 0.29681210643194234 4.311507660317253E-4 48-49 1.715804068901764E-4 3.5195980900549E-5 0.0 0.3716607593145723 4.7514574215741154E-4 50-51 1.8477889972788228E-4 3.5195980900549E-5 4.399497612568625E-6 0.4507725253837814 4.7514574215741154E-4 52-53 1.891783973404509E-4 3.5195980900549E-5 8.79899522513725E-6 0.530214253773933 5.367387087333723E-4 54-55 1.9357789495301952E-4 3.5195980900549E-5 8.79899522513725E-6 0.6243635026829016 5.983316753093331E-4 56-57 1.9357789495301952E-4 3.5195980900549E-5 8.79899522513725E-6 0.7377165586707323 5.983316753093331E-4 58-59 1.9357789495301952E-4 3.5195980900549E-5 8.79899522513725E-6 0.8670573889826373 6.071306705344704E-4 60-61 1.9797739256558817E-4 3.5195980900549E-5 8.79899522513725E-6 1.0023287420762848 6.247286609847449E-4 62-63 2.1117588540329403E-4 3.5195980900549E-5 8.79899522513725E-6 1.142866293812177 6.687236371104311E-4 64-65 2.1117588540329403E-4 4.3994976125686255E-5 8.79899522513725E-6 1.290605823139844 6.687236371104311E-4 66-67 2.1557538301586265E-4 4.3994976125686255E-5 8.79899522513725E-6 1.4620850415849311 6.81922129948137E-4 68-69 2.1997488062843127E-4 4.3994976125686255E-5 8.79899522513725E-6 1.6644487332702504 6.863216275607056E-4 70-71 2.1997488062843127E-4 4.3994976125686255E-5 8.79899522513725E-6 1.8887527195494491 6.907211251732742E-4 72-73 2.903668424295293E-4 4.3994976125686255E-5 8.79899522513725E-6 2.1115520776451495 8.447035416131761E-4 74-75 2.903668424295293E-4 4.3994976125686255E-5 8.79899522513725E-6 2.337061526270192 8.447035416131761E-4 76-77 3.035653352672352E-4 4.3994976125686255E-5 8.79899522513725E-6 2.587551322339399 8.57902034450882E-4 78-79 3.079648328798038E-4 4.3994976125686255E-5 8.79899522513725E-6 2.8787760668033795 8.711005272885879E-4 80-81 3.079648328798038E-4 4.3994976125686255E-5 8.79899522513725E-6 3.195443105960844 8.93098015351431E-4 82-83 3.079648328798038E-4 4.3994976125686255E-5 8.79899522513725E-6 3.523223276087657 9.194950010268428E-4 84-85 3.079648328798038E-4 4.839447373825488E-5 8.79899522513725E-6 3.8518041547799573 9.238944986394114E-4 86-87 3.079648328798038E-4 5.279397135082351E-5 8.79899522513725E-6 4.197846639496543 9.238944986394114E-4 88 3.1676382810494103E-4 5.279397135082351E-5 8.79899522513725E-6 4.482771303379325 9.414924890896859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16340 0.0 38.16316 1 TACCTGG 10875 0.0 32.561127 2 GTATCAA 24715 0.0 30.406681 1 GTACCTG 11950 0.0 30.105537 1 GTACATG 36345 0.0 26.874763 1 TCAACGC 28075 0.0 26.093437 4 TACATGG 36755 0.0 25.921297 2 CAACGCA 28505 0.0 25.716188 5 ATCAACG 28545 0.0 25.56537 3 ACATGGG 36950 0.0 24.938704 3 AACGCAG 29715 0.0 24.70043 6 ACCTGGG 14135 0.0 24.301453 3 TATCAAC 30985 0.0 23.873217 2 CATGGGG 21550 0.0 22.960749 4 ACGCAGA 34325 0.0 21.259754 7 ATGGGGG 13285 0.0 20.621016 5 CGCAGAG 35345 0.0 20.380342 8 GAGTACT 20950 0.0 17.51788 12-13 GCAGAGT 41145 0.0 17.096071 9 CCTGGGG 13600 0.0 16.584686 4 >>END_MODULE