FastQCFastQC Report
Mon 6 Jun 2016
ERR1211502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9579380
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233700.24396150899118732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203810.21275907209026054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132940.1387772486319574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137200.044.6760521
GTATCAA221550.034.5514871
TACCTGG82250.029.6125582
TCAACGC255000.029.0609914
GTACCTG89000.028.828661
CAACGCA259700.028.605045
ATCAACG260050.028.4797653
AACGCAG272050.027.4042456
GTACATG274100.027.1544341
TATCAAC279100.026.8035562
TACATGG276350.025.9132842
ACATGGG277300.024.7931393
ACGCAGA313550.023.448767
CATGGGG161550.023.2993854
CGCAGAG317550.022.962148
ACCTGGG107950.021.766293
GCAGAGT359100.019.4563929
ATGGGGG99350.018.6342095
GAGTACT222800.018.54797612-13
AGAGTAC319700.018.12054610-11
Produced by FastQC (version 0.11.5)