##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211502_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9579380 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.954052141161537 31.0 31.0 34.0 27.0 34.0 2 30.988880073658212 31.0 31.0 34.0 27.0 34.0 3 31.093639254314997 31.0 31.0 34.0 27.0 34.0 4 34.59555994229272 37.0 35.0 37.0 30.0 37.0 5 34.686611555236354 37.0 35.0 37.0 31.0 37.0 6 34.80750779277991 37.0 35.0 37.0 31.0 37.0 7 34.71639803411077 37.0 35.0 37.0 31.0 37.0 8 34.670559368142825 37.0 35.0 37.0 30.0 37.0 9 36.19639642649107 39.0 37.0 39.0 30.0 39.0 10-11 36.17970046078139 39.0 36.0 39.0 30.5 39.0 12-13 36.12575960030816 39.0 35.0 39.0 30.0 39.0 14-15 37.266570487860385 40.0 37.0 41.0 31.0 41.0 16-17 37.24828965966482 40.0 37.0 41.0 31.0 41.0 18-19 37.24955284162441 40.0 37.0 41.0 30.5 41.0 20-21 37.18926501506361 40.0 37.0 41.0 30.0 41.0 22-23 37.022052105668635 40.0 36.5 41.0 30.0 41.0 24-25 36.95134956542073 40.0 36.0 41.0 30.0 41.0 26-27 36.80710348686449 40.0 36.0 41.0 29.5 41.0 28-29 36.647989744639005 39.5 36.0 41.0 29.0 41.0 30-31 36.47952560604131 39.0 36.0 41.0 29.0 41.0 32-33 36.342988533704684 39.0 35.0 41.0 28.5 41.0 34-35 36.16928945297086 39.0 35.0 40.5 27.5 41.0 36-37 36.0000401904925 39.0 35.0 40.0 27.0 41.0 38-39 35.87368608406807 39.0 35.0 40.0 27.0 41.0 40-41 35.836578567715236 39.0 35.0 40.0 27.0 41.0 42-43 35.78716774989613 39.0 35.0 40.0 26.5 41.0 44-45 35.60790489572394 38.5 34.5 40.0 26.0 41.0 46-47 35.583469076286775 38.5 34.0 40.0 26.0 41.0 48-49 35.52617497165787 39.0 34.0 40.0 25.0 41.0 50-51 34.599150414745004 38.0 33.0 39.5 24.0 40.5 52-53 34.82513722182438 38.0 33.5 39.5 24.0 40.5 54-55 35.416776398890114 39.0 34.0 40.0 25.5 41.0 56-57 35.393745367654276 39.0 34.0 40.0 25.5 41.0 58-59 35.240575642682515 38.0 34.0 40.0 25.0 41.0 60-61 35.06400382905783 38.0 34.0 40.0 25.0 41.0 62-63 34.82472195486555 38.0 34.0 40.0 25.0 41.0 64-65 34.55031395560047 37.0 33.0 40.0 23.5 41.0 66-67 34.259577864120644 37.0 33.0 40.0 23.0 41.0 68-69 33.989928993316894 36.0 33.0 39.0 23.0 41.0 70-71 33.645348185373166 36.0 32.0 39.0 23.0 41.0 72-73 33.31044556119498 35.0 32.0 39.0 22.5 40.0 74-75 32.93896530881957 35.0 32.0 37.5 22.0 40.0 76-77 32.58815053792625 35.0 31.0 37.0 22.0 39.0 78-79 32.26740932085375 35.0 31.0 37.0 21.5 39.0 80-81 31.90886435239024 35.0 31.0 36.0 20.5 38.5 82-83 31.597812488908467 35.0 31.0 36.0 20.0 37.0 84-85 31.33119267635275 34.5 30.5 35.0 20.0 37.0 86-87 31.019702162352885 34.0 30.0 35.0 20.0 36.5 88-89 30.787412911900354 34.0 30.0 35.0 19.0 36.0 90-91 30.54657764907541 34.0 29.5 35.0 18.0 36.0 92-93 30.39151672655224 34.0 29.0 35.0 18.0 36.0 94-95 30.237858556608046 34.0 29.0 35.0 18.0 35.0 96-97 30.141733546429936 34.0 29.0 35.0 18.0 35.0 98-99 29.95365848311686 34.0 29.0 35.0 17.0 35.0 100 28.58134931488259 32.0 25.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45495179778280814 1101 2 0.4342402549297333 1101 3 0.4080616857937365 1101 4 0.46837784599485843 1101 5 0.4759058678803427 1101 6 0.4433046720714344 1101 7 0.45455006438643863 1101 8 0.4891360330335459 1101 9 0.4587957706335928 1101 10-11 0.4891539009248973 1101 12-13 0.45492363084088794 1101 14-15 0.5571677898129721 1101 16-17 0.5527738817532253 1101 18-19 0.5097237346396142 1101 20-21 0.5465727151564082 1101 22-23 0.5505183837878675 1101 24-25 0.5675568735607612 1101 26-27 0.4797578033604424 1101 28-29 0.5032466009401446 1101 30-31 0.5496873041918207 1101 32-33 0.42835099584031866 1101 34-35 0.4243430106112882 1101 36-37 0.39217116058947 1101 38-39 0.47453354490359345 1101 40-41 0.42710624662072405 1101 42-43 0.3422321662958723 1101 44-45 0.38303412900032896 1101 46-47 0.24999073163421315 1101 48-49 0.40779622169876717 1101 50-51 0.3354723410537872 1101 52-53 0.4551900755744356 1101 54-55 0.4453795969896248 1101 56-57 0.4672910009470428 1101 58-59 0.40706476484096044 1101 60-61 0.42251267574638973 1101 62-63 0.42195719692496425 1101 64-65 0.41731947682804815 1101 66-67 0.4282903324539049 1101 68-69 0.3482480794538034 1101 70-71 0.31320686459977765 1101 72-73 0.2621059196878903 1101 74-75 0.19388357380432808 1101 76-77 0.16764528898789166 1101 78-79 0.09960749895212473 1101 80-81 0.18299385218688258 1101 82-83 0.13866994868562443 1101 84-85 0.03393126822868808 1101 86-87 0.004733249668795736 1101 88-89 -0.027572372932720413 1101 90-91 -0.015346445152463417 1101 92-93 -0.017618758060713446 1101 94-95 -0.03580725512014027 1101 96-97 -0.1206641174069567 1101 98-99 -0.23137059557942763 1101 100 -0.19616506635833986 1102 1 0.43127682840755455 1102 2 0.45037908710613195 1102 3 0.40522787607548594 1102 4 0.40842031230763354 1102 5 0.47569026967698846 1102 6 0.48412786521149087 1102 7 0.4343254829246135 1102 8 0.5017713944055302 1102 9 0.5292686166932086 1102 10-11 0.48843687356679055 1102 12-13 0.5035063176518335 1102 14-15 0.5346651356684262 1102 16-17 0.5356967073963474 1102 18-19 0.574864608465667 1102 20-21 0.5362031765692308 1102 22-23 0.6230447210683892 1102 24-25 0.5958378045529997 1102 26-27 0.5413515150795121 1102 28-29 0.5125422318098813 1102 30-31 0.5078289946777303 1102 32-33 0.4631631640722489 1102 34-35 0.5482217366219047 1102 36-37 0.5548939045666046 1102 38-39 0.5246033693217242 1102 40-41 0.5206178840237428 1102 42-43 0.4594444978597707 1102 44-45 0.42575728130739066 1102 46-47 0.3174023282042384 1102 48-49 0.49210859409566154 1102 50-51 0.45492436228694544 1102 52-53 0.5537184545046117 1102 54-55 0.6359778003379262 1102 56-57 0.6612652761614157 1102 58-59 0.6191288726400686 1102 60-61 0.5466486496132745 1102 62-63 0.5759494386562807 1102 64-65 0.623807431095095 1102 66-67 0.5237190788810082 1102 68-69 0.5600753558931686 1102 70-71 0.5754726873845897 1102 72-73 0.518121844555175 1102 74-75 0.5277475999374488 1102 76-77 0.45189232859589623 1102 78-79 0.3703441261468967 1102 80-81 0.4374083070664341 1102 82-83 0.397741814753406 1102 84-85 0.31149721351247806 1102 86-87 0.2564804934417637 1102 88-89 0.33964200028083624 1102 90-91 0.2003211742840385 1102 92-93 0.19658214026513576 1102 94-95 0.14617151172346965 1102 96-97 -0.03681683280099435 1102 98-99 0.06416472495956782 1102 100 0.06006196508306516 1103 1 0.42477205184836464 1103 2 0.3801251752092085 1103 3 0.4021210555289194 1103 4 0.47536639185531016 1103 5 0.34891193146414423 1103 6 0.454804389048995 1103 7 0.432617773370616 1103 8 0.4929119383532736 1103 9 0.46363162512657397 1103 10-11 0.5158211409282956 1103 12-13 0.4432849037198636 1103 14-15 0.5822425136626563 1103 16-17 0.600787541913391 1103 18-19 0.5723399078734559 1103 20-21 0.5686018987940358 1103 22-23 0.6733084263899727 1103 24-25 0.6025178663029109 1103 26-27 0.5985830256498375 1103 28-29 0.6283920793852431 1103 30-31 0.61684226184925 1103 32-33 0.5794433737803786 1103 34-35 0.5554642358575776 1103 36-37 0.5538514684899596 1103 38-39 0.5366130520293595 1103 40-41 0.49968033723636296 1103 42-43 0.41355094473636456 1103 44-45 0.44378022237950887 1103 46-47 0.49326652370032065 1103 48-49 0.5761602637210714 1103 50-51 0.47938687765208243 1103 52-53 0.5412599249525982 1103 54-55 0.6277452341813614 1103 56-57 0.5611921676926954 1103 58-59 0.5117310625726148 1103 60-61 0.4748790970484791 1103 62-63 0.5387472429362461 1103 64-65 0.5059933325393189 1103 66-67 0.5605079830214592 1103 68-69 0.40961866072105835 1103 70-71 0.43569497195503715 1103 72-73 0.42416064332571324 1103 74-75 0.37388546528791977 1103 76-77 0.3990400741832545 1103 78-79 0.3310116441604336 1103 80-81 0.35642054004022583 1103 82-83 0.4045401524881598 1103 84-85 0.3999119293156994 1103 86-87 0.3632621330351782 1103 88-89 0.39369789476750583 1103 90-91 0.3311100457126308 1103 92-93 0.3014035297890345 1103 94-95 0.26177708229991836 1103 96-97 0.1916105802326662 1103 98-99 0.19784661228234057 1103 100 0.24207950618361807 1104 1 0.4502629850006272 1104 2 0.48997089081986545 1104 3 0.4048249667184969 1104 4 0.43188796078352 1104 5 0.3363164152427487 1104 6 0.32258757556402884 1104 7 0.3722348270310576 1104 8 0.4375745928431556 1104 9 0.5148310125069528 1104 10-11 0.4825614461937988 1104 12-13 0.4529907853647259 1104 14-15 0.5151165909910347 1104 16-17 0.5355139218648048 1104 18-19 0.43122749999344023 1104 20-21 0.44000775599813124 1104 22-23 0.5250936225877396 1104 24-25 0.5483134632935673 1104 26-27 0.6139666281739338 1104 28-29 0.566643157537797 1104 30-31 0.4835033950672951 1104 32-33 0.40640084143043254 1104 34-35 0.4231875545114079 1104 36-37 0.49539267429918965 1104 38-39 0.5035487712133744 1104 40-41 0.47909866479910335 1104 42-43 0.2584118616350324 1104 44-45 0.39260346309840344 1104 46-47 0.387929712282137 1104 48-49 0.5000321912687511 1104 50-51 0.586878016632042 1104 52-53 0.5685640900826527 1104 54-55 0.5674655607874755 1104 56-57 0.5543106865554321 1104 58-59 0.5942592809857885 1104 60-61 0.6395277020883654 1104 62-63 0.6969460557429557 1104 64-65 0.5721683490420517 1104 66-67 0.5415429097405067 1104 68-69 0.42884325918255684 1104 70-71 0.45867310374315196 1104 72-73 0.47977850848219106 1104 74-75 0.4392577911892701 1104 76-77 0.45410588755984804 1104 78-79 0.34404839820218314 1104 80-81 0.3948462642299333 1104 82-83 0.4705426866329745 1104 84-85 0.28408831900114606 1104 86-87 0.3045624563044349 1104 88-89 0.3946937385005427 1104 90-91 0.3515895477803461 1104 92-93 0.4223126511546127 1104 94-95 0.3783499790808058 1104 96-97 0.447808003914826 1104 98-99 0.372201609793656 1104 100 0.28456966488235835 1105 1 0.3916485714501192 1105 2 0.41720728084889913 1105 3 0.342404066575952 1105 4 0.35097702963100375 1105 5 0.3121039791036253 1105 6 0.3056197416166171 1105 7 0.3467274874414059 1105 8 0.36478575535110025 1105 9 0.422555708155663 1105 10-11 0.4130204112432452 1105 12-13 0.3508125723355846 1105 14-15 0.5260880112221855 1105 16-17 0.5179903344399932 1105 18-19 0.5197809764770014 1105 20-21 0.5600691869588701 1105 22-23 0.6035019262662686 1105 24-25 0.5725016248602017 1105 26-27 0.5438472251484825 1105 28-29 0.5673897773983612 1105 30-31 0.6342659711812431 1105 32-33 0.5952029842163142 1105 34-35 0.5701676931771118 1105 36-37 0.6226404341322009 1105 38-39 0.6424389398086348 1105 40-41 0.6204655570611592 1105 42-43 0.5714505491350295 1105 44-45 0.5482470624520914 1105 46-47 0.5964393342759067 1105 48-49 0.6229064600907535 1105 50-51 0.6492542409045825 1105 52-53 0.6211981875755654 1105 54-55 0.6819310418740443 1105 56-57 0.6479345054012526 1105 58-59 0.6372089819402689 1105 60-61 0.6559030376799697 1105 62-63 0.679201496956523 1105 64-65 0.6813653974179843 1105 66-67 0.607460342256779 1105 68-69 0.5443926629482334 1105 70-71 0.5633974007519456 1105 72-73 0.5953745350021151 1105 74-75 0.5922269761513945 1105 76-77 0.5376298857567434 1105 78-79 0.5545349171969249 1105 80-81 0.650059917766022 1105 82-83 0.6216995068061948 1105 84-85 0.5675695841135742 1105 86-87 0.5768522007986263 1105 88-89 0.654198355649239 1105 90-91 0.5498763265345339 1105 92-93 0.5874295023271543 1105 94-95 0.5057359779418569 1105 96-97 0.4883396438987937 1105 98-99 0.45076212907973456 1105 100 0.5092085670396607 1106 1 0.43372349913126484 1106 2 0.3700836185608303 1106 3 0.3480621143942315 1106 4 0.42935507031647546 1106 5 0.4032511272090247 1106 6 0.3655988025995569 1106 7 0.46246836857419993 1106 8 0.4342517005926112 1106 9 0.4558947410048617 1106 10-11 0.4789799367017622 1106 12-13 0.49460925297750435 1106 14-15 0.5148872055793206 1106 16-17 0.5299118935514997 1106 18-19 0.5509431978039032 1106 20-21 0.496015453550541 1106 22-23 0.6082047169535016 1106 24-25 0.6022487451883549 1106 26-27 0.6161162862138383 1106 28-29 0.6252959943884804 1106 30-31 0.7067918801537942 1106 32-33 0.6579326251834914 1106 34-35 0.6933909091954504 1106 36-37 0.7187342520016742 1106 38-39 0.7056057304226826 1106 40-41 0.6585316127413066 1106 42-43 0.5968297811980889 1106 44-45 0.6481220608698024 1106 46-47 0.6551017755454964 1106 48-49 0.6514188452399523 1106 50-51 0.6602681051215313 1106 52-53 0.6953723555267644 1106 54-55 0.7502040223161259 1106 56-57 0.7339433546094511 1106 58-59 0.6512279967397774 1106 60-61 0.6574941815550659 1106 62-63 0.6786166820678119 1106 64-65 0.7660882769269719 1106 66-67 0.6889380911351921 1106 68-69 0.6478126031593092 1106 70-71 0.5458743075584351 1106 72-73 0.5281958951261245 1106 74-75 0.6086005163619035 1106 76-77 0.6085920820746509 1106 78-79 0.505641988018148 1106 80-81 0.5547258633703223 1106 82-83 0.5517114811131094 1106 84-85 0.5368344518547765 1106 86-87 0.6689595106288451 1106 88-89 0.6342161977234078 1106 90-91 0.6363456751993546 1106 92-93 0.6254144128588877 1106 94-95 0.7483085646480738 1106 96-97 0.7038064780202866 1106 98-99 0.6734503983443219 1106 100 0.6017319885205552 1107 1 0.30541847461189064 1107 2 0.26612653486340676 1107 3 0.27031219995984657 1107 4 0.30100294174924613 1107 5 0.30762600157296305 1107 6 0.2864294996673564 1107 7 0.2971054642316062 1107 8 0.2892506833742914 1107 9 0.25975227275679913 1107 10-11 0.37911376206461966 1107 12-13 0.40749449451109143 1107 14-15 0.3862958504457268 1107 16-17 0.429044201948372 1107 18-19 0.36850851288988196 1107 20-21 0.4085733927086892 1107 22-23 0.5276793026349864 1107 24-25 0.5028560706831868 1107 26-27 0.5514025686458055 1107 28-29 0.5330059229446391 1107 30-31 0.5052434306081324 1107 32-33 0.5258804702878592 1107 34-35 0.6195628616124154 1107 36-37 0.6262122992578938 1107 38-39 0.6177146649014915 1107 40-41 0.6106888602044691 1107 42-43 0.6702501664957268 1107 44-45 0.7359102926613446 1107 46-47 0.610068924346912 1107 48-49 0.6606113787564425 1107 50-51 0.5846024268761383 1107 52-53 0.620971545633644 1107 54-55 0.635843514349844 1107 56-57 0.6684455466997647 1107 58-59 0.6102322454709892 1107 60-61 0.6241271296303026 1107 62-63 0.6537934553381035 1107 64-65 0.6773541567696029 1107 66-67 0.6959383321812282 1107 68-69 0.6156114606536605 1107 70-71 0.6867827984101069 1107 72-73 0.6541406968642391 1107 74-75 0.6256344587031819 1107 76-77 0.6217818556578223 1107 78-79 0.5747036989706018 1107 80-81 0.6637993294445224 1107 82-83 0.5741255615440508 1107 84-85 0.5503789883276973 1107 86-87 0.5924603468099292 1107 88-89 0.5633808407916483 1107 90-91 0.645199551501964 1107 92-93 0.5918419967197579 1107 94-95 0.6007439483732142 1107 96-97 0.5380310257793077 1107 98-99 0.5388345096166134 1107 100 0.7270227583038 1108 1 0.35430498258942933 1108 2 0.37545298638118396 1108 3 0.3643340608728991 1108 4 0.37015524101553154 1108 5 0.3309393352961365 1108 6 0.2945173178929963 1108 7 0.33363687632668615 1108 8 0.3803571324947441 1108 9 0.39298705244183196 1108 10-11 0.34344268984614246 1108 12-13 0.3294464150841705 1108 14-15 0.46696916244798103 1108 16-17 0.4253304500418693 1108 18-19 0.4322016822303425 1108 20-21 0.5021198543483507 1108 22-23 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-0.4161299106770642 2316 66-67 -0.2879321869417666 2316 68-69 -0.2687107200561982 2316 70-71 -0.3775342746407233 2316 72-73 -0.506865497216971 2316 74-75 -0.2744644253790156 2316 76-77 -0.3259766587458728 2316 78-79 -0.573766633346775 2316 80-81 -0.2091461233133174 2316 82-83 -0.15166261562634276 2316 84-85 -0.22027936876600407 2316 86-87 -0.10177393693798109 2316 88-89 -0.1939953414913127 2316 90-91 -0.20942755989317519 2316 92-93 -0.13751785973486363 2316 94-95 -0.1250911465043778 2316 96-97 -0.23539531381365109 2316 98-99 -0.09800426238220439 2316 100 -0.3697494273219988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15.0 1 92.0 2 141.0 3 167.0 4 177.0 5 182.0 6 269.0 7 1054.0 8 2273.0 9 3844.0 10 7775.0 11 16879.0 12 28748.0 13 37369.0 14 41505.0 15 44321.0 16 48090.0 17 53187.0 18 60088.0 19 67712.0 20 76697.0 21 87304.0 22 97576.0 23 104056.0 24 106861.0 25 112974.0 26 121977.0 27 137545.0 28 157999.0 29 182992.0 30 216545.0 31 260537.0 32 323265.0 33 413357.0 34 555420.0 35 780961.0 36 1182546.0 37 1844624.0 38 1932909.0 39 468607.0 40 740.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01692009695154 18.56379189169465 12.32809367604711 24.0911943353067 2 15.387258274447582 20.16003437428683 40.53553500417557 23.917172347090016 3 18.28995392020744 25.584017095302304 30.33190232019608 25.794126664294176 4 11.975475129168679 16.385463686261765 38.73125333768856 32.907807846881 5 12.48481332357209 38.015961947449064 36.01754934997631 13.481675379002533 6 31.125890243385562 37.510045283899714 18.080091265149353 13.283973207565369 7 26.93295350221537 32.54748880727976 22.86554308312543 17.65401460737944 8 25.226303805257157 36.299830866556 20.739741932910995 17.73412339527585 9 26.16242354897694 15.014555512694901 20.345960290619587 38.47706064770857 10-11 24.2320289293906 26.09826964636578 29.672716381374958 19.996985042868662 12-13 24.76760404907105 23.900826646450856 29.387622451471852 21.943946853006242 14-15 22.1015055090454 25.53700108820972 26.652435629484554 25.70905777326032 16-17 21.85579057202665 28.354379580580503 27.459076915463758 22.33075293192909 18-19 21.677779448890295 27.698013042577852 29.013959334750115 21.610248173781734 20-21 22.384904068560157 27.0572210854175 29.005331105143867 21.55254374087848 22-23 22.10500410095166 26.914723024800537 28.773067428814798 22.207205445433008 24-25 21.87472184281909 26.998039737433714 28.941325090301078 22.185913329446116 26-27 21.887058498534703 27.2191865551335 28.775040686441884 22.11871425988991 28-29 21.849827774565455 27.257531518102923 28.877800213274995 22.014840494056624 30-31 22.164686278353425 27.183115465822148 28.806559699682943 21.845638556141484 32-33 21.861185370367092 27.288395837708883 28.753124748919834 22.097294043004187 34-35 22.04742706293701 27.354080715634648 28.78261545068438 21.815876770743962 36-37 22.07497416949396 27.28037648455257 28.678014252080576 21.966635093872895 38-39 21.977448575242274 27.389535753007472 28.67701322100325 21.956002450747004 40-41 22.091602689966006 27.252680505765735 28.76157835229767 21.89413845197059 42-43 21.93668572229376 27.27569346830775 28.6522787779747 22.135342031423797 44-45 21.96027838381661 27.50824888124744 28.55640714280642 21.97506559212953 46-47 21.950505301623654 27.376098542509 28.35521604151629 22.31818011435106 48-49 21.942173990030604 27.42199610946291 28.456808606957196 22.17902129354929 50-51 21.985736448417523 27.590527416480953 28.32381317993343 22.099922955168093 52-53 22.010512102826212 27.631819855529454 28.21370944143497 22.14395860020936 54-55 21.9192551237262 27.605927496836784 28.33678565359275 22.138031725844264 56-57 21.979198272947542 27.629665191469066 28.16667004264381 22.22446649293958 58-59 21.985617616594972 27.61044529483343 28.298139971762332 22.105797116809264 60-61 22.09528645569841 27.6652882443791 28.22696321802138 22.01246208190111 62-63 21.907494090470312 27.6957338326417 28.350478128147167 22.046293948740818 64-65 21.877051777672577 27.524922335605734 28.321968209118776 22.27605767760292 66-67 21.903300700187124 27.580691297286464 28.181935363221843 22.334072639304566 68-69 21.865602388496338 27.628355207381684 28.24748090887248 22.2585614952495 70-71 21.84100741329602 27.43170171615069 28.264924043336315 22.46236682721698 72-73 21.80483753379328 27.6012090858137 28.14189631266214 22.452057067730884 74-75 21.85538718461474 27.657312033798092 28.07545941828435 22.411841363302816 76-77 21.784250074486845 27.51609043754933 28.188792657390426 22.510866830573402 78-79 21.786857372257515 27.469602772671912 28.188935518881493 22.55460433618908 80-81 21.803915250511096 27.629966220260528 27.99941694800787 22.566701581220507 82-83 21.87157811461362 27.59973757507951 28.063481271110135 22.465203039196734 84-85 21.91189185650516 27.573818037997317 27.985028771908873 22.52926133358865 86-87 21.746842741416785 27.530380182464864 28.1658674849352 22.556909591183146 88-89 21.755722839612645 27.38076643096362 28.194181040153328 22.66932968927041 90-91 21.89126803076107 27.474312217021403 28.12953009191011 22.504889660307423 92-93 21.826195634513397 27.410069756397675 28.134918546530784 22.628816062558137 94-95 21.661731447691437 27.3361464925506 28.204730762265147 22.797391297492815 96-97 21.71615406436468 27.262822835878413 28.25191824548468 22.76910485427223 98-99 21.763051674410395 27.169849696719993 28.15931092085224 22.90778770801737 100 21.851126223974866 27.13242113090265 28.12393476589914 22.89251787922334 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2094.0 1 1529.5 2 1761.0 3 2732.5 4 2283.0 5 1984.0 6 3064.5 7 5579.0 8 8617.5 9 10026.0 10 8559.5 11 6785.0 12 6767.5 13 7151.0 14 7650.5 15 8102.0 16 8581.0 17 9325.0 18 10348.0 19 11706.5 20 13641.0 21 16502.5 22 20333.5 23 25477.0 24 32321.5 25 40847.5 26 51310.5 27 63732.0 28 78513.0 29 95906.0 30 115796.5 31 135321.0 32 155995.5 33 179014.0 34 201992.5 35 224051.0 36 249025.0 37 272507.5 38 289950.5 39 304723.0 40 316571.0 41 326748.5 42 335931.5 43 346782.5 44 357252.0 45 366896.5 46 374519.0 47 378131.0 48 381646.0 49 382979.0 50 378117.0 51 362464.0 52 340882.5 53 322598.5 54 302343.5 55 275023.5 56 243526.5 57 212476.5 58 183973.5 59 155912.5 60 128396.0 61 103980.0 62 82099.5 63 62054.0 64 45404.5 65 32972.5 66 23784.5 67 17398.5 68 12811.5 69 9293.0 70 6831.0 71 5093.5 72 3685.0 73 2688.5 74 1977.0 75 1378.0 76 961.0 77 689.5 78 508.0 79 386.5 80 301.0 81 234.5 82 200.0 83 182.0 84 151.0 85 113.0 86 80.5 87 71.0 88 62.0 89 50.0 90 43.0 91 35.5 92 29.5 93 24.5 94 20.0 95 14.0 96 12.0 97 8.5 98 6.0 99 6.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15673248164286205 2 0.09850324342493982 3 0.03077443425357382 4 0.03279961751178051 5 0.028488273771371427 6 0.002693284951635701 7 0.004029488338493723 8 0.005772816194785049 9 0.005584912593508139 10-11 0.004916810900079128 12-13 0.007709267196833199 14-15 0.01798133073330424 16-17 0.008680102470097229 18-19 0.016655566435406048 20-21 0.021895989093239854 22-23 0.043463146884245116 24-25 0.019171386874724666 26-27 0.009849280433597998 28-29 0.026541383680363444 30-31 0.01568473116214202 32-33 0.020987788353734795 34-35 0.020658957051500202 36-37 0.021311400111489468 38-39 0.01975075631199514 40-41 0.0256384024853383 42-43 0.0343811394891945 44-45 0.03691261856195286 46-47 0.02120178967741127 48-49 0.013236764801062282 50-51 0.01242773540667559 52-53 0.022271796295793674 54-55 0.02596723378757289 56-57 0.019896903557432736 58-59 0.025633182940858385 60-61 0.02598289242101263 62-63 0.0501337247295754 64-65 0.03215761354075107 66-67 0.022491017163950067 68-69 0.029344279066077342 70-71 0.04263845885641868 72-73 0.040514104253093625 74-75 0.041009960978685464 76-77 0.022814628921704744 78-79 0.0168591286701227 80-81 0.022856385277544057 82-83 0.01861811515985377 84-85 0.018884311928329393 86-87 0.021755061392282175 88-89 0.01870684741601231 90-91 0.02917203409824018 92-93 0.02739216943058945 94-95 0.02221960085099453 96-97 0.01552292528326468 98-99 0.019254899586403296 100 0.04196513761850976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9579380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.93076441754015 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88825517670841 46.49844379748528 2 16.15043346425767 20.327182471607127 3 5.184094845724008 9.787171543633251 4 2.0297589885834912 5.10937138939729 5 0.9261669158298467 2.9142195995703912 6 0.5253812994111656 1.9837588069575116 7 0.327435297390301 1.4424027493439362 8 0.22198646784625148 1.1175822489531442 9 0.14073496907670022 0.7970903265848058 >10 0.567099173639297 5.99154075553954 >50 0.025325134934999143 1.099914547412104 >100 0.012017774418611944 1.3646969750920874 >500 9.107466719365511E-4 0.3539658368452473 >1k 3.331212578085678E-4 0.4154142042567016 >5k 0.0 0.0 >10k+ 6.66242494683191E-5 0.7972447473215486 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23370 0.24396150899118732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20381 0.21275907209026054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13294 0.1387772486319574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0439088959828298E-5 0.0 0.0 1.0439088959828298E-5 3 0.0 1.0439088959828298E-5 0.0 0.0 1.0439088959828298E-5 4 0.0 1.0439088959828298E-5 0.0 2.0878177919656597E-5 1.0439088959828298E-5 5 0.0 1.0439088959828298E-5 0.0 2.0878177919656597E-5 1.0439088959828298E-5 6 1.0439088959828298E-5 1.0439088959828298E-5 0.0 2.0878177919656597E-5 1.0439088959828298E-5 7 1.0439088959828298E-5 1.0439088959828298E-5 0.0 2.0878177919656597E-5 1.0439088959828298E-5 8 1.0439088959828298E-5 1.0439088959828298E-5 0.0 4.175635583931319E-5 1.0439088959828298E-5 9 1.0439088959828298E-5 1.0439088959828298E-5 0.0 5.2195444799141486E-5 1.0439088959828298E-5 10-11 2.0878177919656597E-5 1.0439088959828298E-5 0.0 6.263453375896979E-5 1.0439088959828298E-5 12-13 2.0878177919656597E-5 1.0439088959828298E-5 0.0 6.785407823888394E-5 1.0439088959828298E-5 14-15 3.131726687948489E-5 3.131726687948489E-5 0.0 7.829316719871224E-5 4.6975900319227336E-5 16-17 4.6975900319227336E-5 5.2195444799141486E-5 0.0 9.395180063845469E-5 5.2195444799141486E-5 18-19 5.2195444799141486E-5 5.2195444799141486E-5 0.0 9.395180063845469E-5 5.2195444799141486E-5 20-21 5.2195444799141486E-5 5.2195444799141486E-5 0.0 9.395180063845469E-5 6.263453375896979E-5 22-23 8.873225615854054E-5 5.2195444799141486E-5 0.0 1.0439088959828297E-4 6.263453375896979E-5 24-25 1.0439088959828297E-4 5.2195444799141486E-5 0.0 1.9834269023673767E-4 6.263453375896979E-5 26-27 1.1482997855811127E-4 5.2195444799141486E-5 0.0 3.027335798350206E-4 6.263453375896979E-5 28-29 1.4614724543759617E-4 5.2195444799141486E-5 0.0 0.0010386893515029157 6.263453375896979E-5 30-31 1.6180587887733864E-4 5.2195444799141486E-5 0.0 0.004081683783292865 6.263453375896979E-5 32-33 1.8268405679699524E-4 5.7414989279055636E-5 0.0 0.010767920262062889 8.351271167862639E-5 34-35 1.9834269023673767E-4 7.829316719871224E-5 0.0 0.021321839200449298 9.395180063845469E-5 36-37 2.0878177919656595E-4 8.873225615854054E-5 0.0 0.04016439477293937 9.917134511836884E-5 38-39 2.0878177919656595E-4 1.0439088959828297E-4 0.0 0.0697592119740526 1.0961043407819712E-4 40-41 2.2965995711622255E-4 1.2004952303802542E-4 0.0 0.10300771031110573 1.2004952303802542E-4 42-43 2.2965995711622255E-4 1.2526906751793957E-4 0.0 0.13666333311759216 1.3570815647776787E-4 44-45 2.4009904607605085E-4 1.2526906751793957E-4 0.0 0.17883203297081857 1.5658633439742447E-4 46-47 2.6097722399570747E-4 1.2526906751793957E-4 0.0 0.2312206009157169 1.6702542335725277E-4 48-49 2.6097722399570747E-4 1.3570815647776787E-4 0.0 0.2882963198035781 1.6702542335725277E-4 50-51 2.6097722399570747E-4 1.3570815647776787E-4 0.0 0.3489422071157006 1.6702542335725277E-4 52-53 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.41015702477613375 1.8268405679699524E-4 54-55 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.48123156195912464 1.9834269023673767E-4 56-57 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.5691025932784795 1.9834269023673767E-4 58-59 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.6681956452296496 1.9834269023673767E-4 60-61 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.7750971357227712 1.9834269023673767E-4 62-63 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.8834653182147487 1.9834269023673767E-4 64-65 2.81855401915364E-4 1.3570815647776787E-4 0.0 0.9978881723034267 1.9834269023673767E-4 66-67 2.81855401915364E-4 1.3570815647776787E-4 0.0 1.1336746219483933 1.9834269023673767E-4 68-69 2.81855401915364E-4 1.3570815647776787E-4 0.0 1.289399731506632 2.035622347166518E-4 70-71 2.81855401915364E-4 1.3570815647776787E-4 0.0 1.4643953992847136 2.0878177919656595E-4 72-73 2.81855401915364E-4 1.3570815647776787E-4 0.0 1.6408316613392517 2.4531859055596504E-4 74-75 2.81855401915364E-4 1.3570815647776787E-4 0.0 1.818692859036806 2.6097722399570747E-4 76-77 2.870749463952782E-4 1.5658633439742447E-4 0.0 2.0198488837482174 2.6097722399570747E-4 78-79 2.9229449087519234E-4 1.5658633439742447E-4 0.0 2.25013518620203 2.6097722399570747E-4 80-81 2.9229449087519234E-4 1.5658633439742447E-4 0.0 2.502228745492923 2.6097722399570747E-4 82-83 3.027335798350206E-4 1.5658633439742447E-4 0.0 2.7655756426825118 2.870749463952782E-4 84-85 3.1317266879484894E-4 1.5658633439742447E-4 0.0 3.0305562572943137 2.9229449087519234E-4 86-87 3.183922132747631E-4 1.5658633439742447E-4 0.0 3.3094052015892466 2.9229449087519234E-4 88 3.236117577546772E-4 1.5658633439742447E-4 0.0 3.5384022765565204 2.9229449087519234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13720 0.0 44.676052 1 GTATCAA 22155 0.0 34.551487 1 TACCTGG 8225 0.0 29.612558 2 TCAACGC 25500 0.0 29.060991 4 GTACCTG 8900 0.0 28.82866 1 CAACGCA 25970 0.0 28.60504 5 ATCAACG 26005 0.0 28.479765 3 AACGCAG 27205 0.0 27.404245 6 GTACATG 27410 0.0 27.154434 1 TATCAAC 27910 0.0 26.803556 2 TACATGG 27635 0.0 25.913284 2 ACATGGG 27730 0.0 24.793139 3 ACGCAGA 31355 0.0 23.44876 7 CATGGGG 16155 0.0 23.299385 4 CGCAGAG 31755 0.0 22.96214 8 ACCTGGG 10795 0.0 21.76629 3 GCAGAGT 35910 0.0 19.456392 9 ATGGGGG 9935 0.0 18.634209 5 GAGTACT 22280 0.0 18.547976 12-13 AGAGTAC 31970 0.0 18.120546 10-11 >>END_MODULE