FastQCFastQC Report
Mon 6 Jun 2016
ERR1211502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9579380
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256240.2674912155066403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237520.24794924097384174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159770.1667853243111767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152200.044.8323671
GTATCAA236950.033.3618351
TACCTGG84000.027.9348662
TCAACGC285500.027.1099874
ATCAACG287750.026.7836883
CAACGCA291250.026.6390325
GTACCTG90400.026.3741511
AACGCAG304150.025.6631576
GTACATG284750.025.2512381
TATCAAC309400.025.1385672
TACATGG290700.024.0218662
ACATGGG286200.023.529813
ACGCAGA342150.022.5656057
CATGGGG159600.022.1425084
CGCAGAG349500.021.9700418
ACCTGGG110000.020.7200553
TATACCG7150.019.060285
GCAGAGT392200.019.0149569
GAGTACT239850.018.98552112-13
AGAGTAC356300.018.22062910-11
Produced by FastQC (version 0.11.5)