##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211502_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9579380 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12943489035825 33.0 31.0 34.0 30.0 34.0 2 32.37699329184144 34.0 31.0 34.0 31.0 34.0 3 32.402973574490204 34.0 31.0 34.0 31.0 34.0 4 36.02556501568995 37.0 35.0 37.0 35.0 37.0 5 35.94704761686038 37.0 35.0 37.0 35.0 37.0 6 35.96083243383183 37.0 35.0 37.0 35.0 37.0 7 35.88340498028056 37.0 35.0 37.0 35.0 37.0 8 35.87983846553744 37.0 35.0 37.0 35.0 37.0 9 37.55099912520434 39.0 37.0 39.0 35.0 39.0 10-11 37.515489311416815 39.0 37.0 39.0 35.0 39.0 12-13 37.43666693460328 39.0 37.0 39.0 34.0 39.0 14-15 38.69461296033772 40.0 38.0 41.0 34.0 41.0 16-17 38.62993367002875 40.0 38.0 41.0 34.0 41.0 18-19 38.605817077931974 40.0 38.0 41.0 34.0 41.0 20-21 38.540341546112586 40.0 38.0 41.0 34.0 41.0 22-23 38.43655429683341 40.0 38.0 41.0 34.0 41.0 24-25 38.335160626261825 40.0 38.0 41.0 34.0 41.0 26-27 38.17261706916314 40.0 38.0 41.0 33.0 41.0 28-29 38.21311895968215 40.0 38.0 41.0 34.0 41.0 30-31 38.150357225624205 40.0 38.0 41.0 33.5 41.0 32-33 37.99485405109725 40.0 37.0 41.0 33.0 41.0 34-35 37.89540481743077 40.0 37.0 41.0 33.0 41.0 36-37 37.839014529124015 40.0 37.0 41.0 33.0 41.0 38-39 37.74943498431005 40.0 37.0 41.0 33.0 41.0 40-41 37.60141778486708 40.0 37.0 41.0 32.0 41.0 42-43 37.46011051863482 40.0 37.0 41.0 32.0 41.0 44-45 37.292003188097766 40.0 36.5 41.0 31.0 41.0 46-47 37.058276997049916 39.5 36.0 41.0 31.0 41.0 48-49 36.85550703698987 39.0 36.0 41.0 30.0 41.0 50-51 36.66761695433316 39.0 35.5 41.0 30.0 41.0 52-53 36.4729968432195 39.0 35.0 40.0 29.5 41.0 54-55 36.28153961947433 39.0 35.0 40.0 29.0 41.0 56-57 36.0851759717226 38.5 35.0 40.0 28.0 41.0 58-59 35.82826138017283 38.0 34.5 40.0 28.0 41.0 60-61 35.51708435201443 38.0 34.0 40.0 27.5 41.0 62-63 35.200899797272896 38.0 34.0 40.0 27.0 41.0 64-65 34.88601757107453 37.0 34.0 40.0 26.0 41.0 66-67 34.54407790483309 36.5 33.0 39.0 26.0 41.0 68-69 34.14381844127699 36.0 33.0 39.0 26.0 40.0 70-71 33.75362810536799 35.5 32.0 39.0 25.5 40.0 72-73 33.34114911403452 35.0 32.0 38.0 25.0 40.0 74-75 32.9238774325687 35.0 31.5 37.0 24.0 39.5 76-77 31.41720982986373 33.5 29.5 35.5 22.5 38.5 78-79 32.181584820729526 34.0 31.0 36.0 24.0 39.0 80-81 32.116419590829466 34.5 31.0 36.0 24.0 38.0 82-83 31.814939954360305 34.0 31.0 35.5 23.0 37.0 84-85 31.504847077785826 34.0 31.0 35.0 22.0 37.0 86-87 31.18769847317885 34.0 30.0 35.0 20.5 36.0 88-89 30.90269735619633 34.0 30.0 35.0 20.0 36.0 90-91 30.62077524850251 34.0 29.5 35.0 20.0 35.5 92-93 30.399574398343105 34.0 29.0 35.0 19.5 35.0 94-95 30.175078345362643 34.0 29.0 35.0 19.0 35.0 96-97 29.9656044023726 34.0 29.0 35.0 18.0 35.0 98-99 29.78959102781182 34.0 29.0 35.0 18.0 35.0 100 27.821051779969057 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25065883512147735 1101 2 0.18772723579748174 1101 3 0.253878261329632 1101 4 0.11203394539963085 1101 5 0.13111487774700237 1101 6 0.13990727022574845 1101 7 0.18393258885618025 1101 8 0.16199071348474092 1101 9 0.19049425835235212 1101 10-11 0.1959877162255168 1101 12-13 0.19068351904612513 1101 14-15 0.30746006403389714 1101 16-17 0.3071911058598644 1101 18-19 0.3068738635776711 1101 20-21 0.28260169281544734 1101 22-23 0.20494120128569904 1101 24-25 0.2022604791941589 1101 26-27 0.20358068681032648 1101 28-29 0.2361665458738642 1101 30-31 0.29847333779629537 1101 32-33 0.3542367212662825 1101 34-35 0.34254678848714093 1101 36-37 0.39305701022340145 1101 38-39 0.35806547030948366 1101 40-41 0.3493030062247726 1101 42-43 0.3593386780692569 1101 44-45 0.37767092982117134 1101 46-47 0.4336172793612647 1101 48-49 0.44381138285470456 1101 50-51 0.45318811795791447 1101 52-53 0.4249505166290177 1101 54-55 0.41761659482634883 1101 56-57 0.40487282385581835 1101 58-59 0.4336076953536647 1101 60-61 0.4569031618643109 1101 62-63 0.4318306841869628 1101 64-65 0.36472512637872967 1101 66-67 0.2730569340448241 1101 68-69 0.33046315473183085 1101 70-71 0.3135807900008629 1101 72-73 0.151283899848778 1101 74-75 0.2633360808098786 1101 76-77 0.23003829318956903 1101 78-79 0.3970102324758287 1101 80-81 0.40366396297083185 1101 82-83 0.38103169203659704 1101 84-85 0.32718252738438736 1101 86-87 0.31438533558956294 1101 88-89 0.3261429683023529 1101 90-91 0.3007086428848105 1101 92-93 0.21292189989747 1101 94-95 0.32642987392545564 1101 96-97 0.2298029545075302 1101 98-99 0.1517858639487102 1101 100 0.330698886224571 1102 1 0.2935653275673715 1102 2 0.23214863228584193 1102 3 0.2828157868502572 1102 4 0.16568033167974505 1102 5 0.17426065153222225 1102 6 0.14073699664469075 1102 7 0.18877538183290454 1102 8 0.17525000299066562 1102 9 0.1981373779930209 1102 10-11 0.21152507841415513 1102 12-13 0.22760797800896881 1102 14-15 0.32485206076725603 1102 16-17 0.35952307809343864 1102 18-19 0.2732581020423481 1102 20-21 0.30680830776032053 1102 22-23 0.29202205061195485 1102 24-25 0.33019514640526637 1102 26-27 0.2651458154342521 1102 28-29 0.34007058834018267 1102 30-31 0.3386187032515906 1102 32-33 0.42770834233611055 1102 34-35 0.41183588607799493 1102 36-37 0.5000958015668076 1102 38-39 0.4536416238415413 1102 40-41 0.498779118923828 1102 42-43 0.3450401888900032 1102 44-45 0.3640041270445309 1102 46-47 0.45242046433472183 1102 48-49 0.48472685855907116 1102 50-51 0.5168945287378293 1102 52-53 0.4631661148323687 1102 54-55 0.5170367663249138 1102 56-57 0.48132525341890187 1102 58-59 0.5241299493348848 1102 60-61 0.5637304383036792 1102 62-63 0.6012696388623482 1102 64-65 0.6038929499801995 1102 66-67 0.5413652231424138 1102 68-69 0.5658200350911571 1102 70-71 0.5878372211972689 1102 72-73 0.5829715274518747 1102 74-75 0.5662515565142456 1102 76-77 0.5215266557865483 1102 78-79 0.5026378355791294 1102 80-81 0.5174891977605398 1102 82-83 0.49816317427425005 1102 84-85 0.40683258863387906 1102 86-87 0.43772506609180795 1102 88-89 0.4437003386575995 1102 90-91 0.4071774056451183 1102 92-93 0.3893036883825687 1102 94-95 0.5270064357874382 1102 96-97 0.5047890712898919 1102 98-99 0.4335968071908525 1102 100 0.5354616465349018 1103 1 0.2768710009345128 1103 2 0.23824596010513233 1103 3 0.3017972052612379 1103 4 0.1318773148922716 1103 5 0.14613225500420413 1103 6 0.1881941007149308 1103 7 0.19115182225519334 1103 8 0.18760648666808066 1103 9 0.23926522219402813 1103 10-11 0.23186108447340104 1103 12-13 0.30795700338455134 1103 14-15 0.3871564244022423 1103 16-17 0.391298594280137 1103 18-19 0.33443643320957506 1103 20-21 0.32407614861465106 1103 22-23 0.3063249095018463 1103 24-25 0.34944308587325423 1103 26-27 0.3334954799490646 1103 28-29 0.35382590118499024 1103 30-31 0.39414531245443385 1103 32-33 0.43519672396765685 1103 34-35 0.4736186517139487 1103 36-37 0.45459635074844584 1103 38-39 0.46283063920227363 1103 40-41 0.5093939230490534 1103 42-43 0.5215965520368329 1103 44-45 0.46855160263331896 1103 46-47 0.4958837635261091 1103 48-49 0.5641081868866493 1103 50-51 0.566046786044204 1103 52-53 0.49458642562010624 1103 54-55 0.6256402596988764 1103 56-57 0.646771862017836 1103 58-59 0.6523158834909637 1103 60-61 0.6438244974542542 1103 62-63 0.6542222783941156 1103 64-65 0.6510642782952019 1103 66-67 0.6439234508646976 1103 68-69 0.6284341849793691 1103 70-71 0.6398082686936206 1103 72-73 0.6013455622950374 1103 74-75 0.5732115813752614 1103 76-77 0.5959487820819653 1103 78-79 0.6606139383144694 1103 80-81 0.6019593123400853 1103 82-83 0.6361022773908012 1103 84-85 0.5631862641247771 1103 86-87 0.5497252774606842 1103 88-89 0.610313255560694 1103 90-91 0.6353073924723098 1103 92-93 0.6301805370739046 1103 94-95 0.6574081096276494 1103 96-97 0.6383573478409055 1103 98-99 0.6631368911596525 1103 100 0.7384968230971154 1104 1 0.21889352168940235 1104 2 0.1983279395744333 1104 3 0.24426509596814583 1104 4 0.022792204855271336 1104 5 0.10198257760281138 1104 6 0.11029557927357558 1104 7 0.11798855174893674 1104 8 0.16065102894350503 1104 9 0.20624589917294145 1104 10-11 0.21636306185772014 1104 12-13 0.19941052202162268 1104 14-15 0.32232810855573035 1104 16-17 0.2776271316635288 1104 18-19 0.25124237007832306 1104 20-21 0.2050545364431997 1104 22-23 0.26417014131423144 1104 24-25 0.30557671996238156 1104 26-27 0.3076383397883973 1104 28-29 0.3468147004478084 1104 30-31 0.3103168381404231 1104 32-33 0.29549044458615015 1104 34-35 0.4014275094383919 1104 36-37 0.2707964382140915 1104 38-39 0.3709284449078396 1104 40-41 0.4831874028102945 1104 42-43 0.4945850955829201 1104 44-45 0.4638594836674912 1104 46-47 0.3996206024338491 1104 48-49 0.40530041824442975 1104 50-51 0.44916111673608583 1104 52-53 0.4245110612030132 1104 54-55 0.4842477823068023 1104 56-57 0.5873852904686245 1104 58-59 0.6176785249299854 1104 60-61 0.5924027285591862 1104 62-63 0.5688235889869162 1104 64-65 0.7375128578969381 1104 66-67 0.6903196220891488 1104 68-69 0.6560285956911684 1104 70-71 0.541314196517213 1104 72-73 0.6429231824514048 1104 74-75 0.6996696902694453 1104 76-77 0.5554304037865592 1104 78-79 0.680670835087021 1104 80-81 0.7789376824060312 1104 82-83 0.6277108738674251 1104 84-85 0.6826316853213008 1104 86-87 0.8051040889374228 1104 88-89 0.7384601621174447 1104 90-91 0.6129335988499243 1104 92-93 0.6312503141004377 1104 94-95 0.7232224269449752 1104 96-97 0.7398893915458906 1104 98-99 0.66141994240175 1104 100 0.9042237475997617 1105 1 0.18516973628079825 1105 2 0.10634081507819815 1105 3 0.1765309028032931 1105 4 0.09354505228619558 1105 5 0.05336436947244039 1105 6 0.07270640890120461 1105 7 0.13017573970950735 1105 8 0.14283287793267618 1105 9 0.04180814171799341 1105 10-11 0.1038085911106208 1105 12-13 0.16354970325965468 1105 14-15 0.20611324950892396 1105 16-17 0.23596542784388674 1105 18-19 0.2582419415178734 1105 20-21 0.22481186613448045 1105 22-23 0.29372414128422264 1105 24-25 0.2950723060274072 1105 26-27 0.30491975161802287 1105 28-29 0.3610494573073808 1105 30-31 0.3791213975219918 1105 32-33 0.3971862299465485 1105 34-35 0.43467803802771243 1105 36-37 0.4315715633290722 1105 38-39 0.41730918095140623 1105 40-41 0.3919447668487024 1105 42-43 0.3571358877434676 1105 44-45 0.39285708666361074 1105 46-47 0.4518949567056225 1105 48-49 0.4739778870719462 1105 50-51 0.5604997480264586 1105 52-53 0.5643408371211436 1105 54-55 0.49945192357140655 1105 56-57 0.49822421190623345 1105 58-59 0.5514136965373737 1105 60-61 0.5493482852324121 1105 62-63 0.5135649788196233 1105 64-65 0.5425901619073841 1105 66-67 0.5570418699100514 1105 68-69 0.6046839196326204 1105 70-71 0.6437638666904277 1105 72-73 0.5796040513995351 1105 74-75 0.6268758523589071 1105 76-77 0.5960746454071035 1105 78-79 0.6632502480854399 1105 80-81 0.6637283378371137 1105 82-83 0.6363151902614383 1105 84-85 0.6346046844550806 1105 86-87 0.5955074190029954 1105 88-89 0.6230060019783075 1105 90-91 0.6685581822679261 1105 92-93 0.7219946604100791 1105 94-95 0.8210404697500309 1105 96-97 0.789387693821844 1105 98-99 0.8482262111886669 1105 100 0.9277988967987234 1106 1 0.21975205161106715 1106 2 0.1734277119398442 1106 3 0.215155106183893 1106 4 0.15667686501340228 1106 5 0.12990755972939638 1106 6 0.11345018546955998 1106 7 0.13445360033575326 1106 8 0.11536621625570831 1106 9 0.16073622012398658 1106 10-11 0.17464660508592544 1106 12-13 0.19566699933572096 1106 14-15 0.24100538235911984 1106 16-17 0.25929734444792985 1106 18-19 0.28820380911462706 1106 20-21 0.2957467840383856 1106 22-23 0.319224129868104 1106 24-25 0.30924929803876466 1106 26-27 0.32613918328138425 1106 28-29 0.357387524932534 1106 30-31 0.3303006028409641 1106 32-33 0.37750620978477656 1106 34-35 0.39846192610888664 1106 36-37 0.3835132826377148 1106 38-39 0.348596467940709 1106 40-41 0.40849406896888496 1106 42-43 0.4048683998943403 1106 44-45 0.468530946300703 1106 46-47 0.48857040478043956 1106 48-49 0.5343987262580967 1106 50-51 0.522255633410829 1106 52-53 0.6003865846480778 1106 54-55 0.559213664222483 1106 56-57 0.5493561497572799 1106 58-59 0.6932083614356159 1106 60-61 0.62818197500237 1106 62-63 0.6074580931406501 1106 64-65 0.6275685880042161 1106 66-67 0.6938708086024903 1106 68-69 0.5962502611495353 1106 70-71 0.663977097527642 1106 72-73 0.5986384002759166 1106 74-75 0.604162013154891 1106 76-77 0.5993871636773385 1106 78-79 0.7523134959127589 1106 80-81 0.7599461507380667 1106 82-83 0.7826510012805308 1106 84-85 0.732792399101303 1106 86-87 0.6995566210595996 1106 88-89 0.7227658582795264 1106 90-91 0.8241269921116121 1106 92-93 0.8020025296459359 1106 94-95 0.8974063158112848 1106 96-97 0.9006753418286841 1106 98-99 0.934331239727193 1106 100 0.9972834844258003 1107 1 0.1858594425057234 1107 2 0.11423706191139615 1107 3 0.18975113169393154 1107 4 0.11865897908754874 1107 5 0.10445331396661572 1107 6 0.1264321014910621 1107 7 0.18171290960172826 1107 8 0.1317531012758124 1107 9 0.17635555849877704 1107 10-11 0.17596528256868282 1107 12-13 0.1770805439665324 1107 14-15 0.229214814638091 1107 16-17 0.20292946682087631 1107 18-19 0.18041744935519688 1107 20-21 0.2066033169014787 1107 22-23 0.2586030397483583 1107 24-25 0.2474199789833733 1107 26-27 0.29704556017886574 1107 28-29 0.3325296945854106 1107 30-31 0.21931965868581216 1107 32-33 0.23350017291921432 1107 34-35 0.27886323238028154 1107 36-37 0.3229159467051943 1107 38-39 0.275983556539245 1107 40-41 0.3513038921373237 1107 42-43 0.3043186437672958 1107 44-45 0.36725987153526063 1107 46-47 0.3738009541612186 1107 48-49 0.45016678269184496 1107 50-51 0.3736859623123152 1107 52-53 0.4316631198441385 1107 54-55 0.4998332502473133 1107 56-57 0.4869781932117334 1107 58-59 0.5705410984321517 1107 60-61 0.5502061093913682 1107 62-63 0.5511064222711184 1107 64-65 0.6180830187908768 1107 66-67 0.6721857964029496 1107 68-69 0.49928384943548565 1107 70-71 0.6054012260808506 1107 72-73 0.5265513156060848 1107 74-75 0.5375151764612554 1107 76-77 0.5193939105403835 1107 78-79 0.6684548805655552 1107 80-81 0.6545728794616821 1107 82-83 0.6312155980803276 1107 84-85 0.5689771221736386 1107 86-87 0.6129824135515101 1107 88-89 0.530509238122324 1107 90-91 0.3999889709169473 1107 92-93 0.6108363109169765 1107 94-95 0.7509746900322583 1107 96-97 0.7375570228623083 1107 98-99 0.8495349236276297 1107 100 0.960154308347029 1108 1 0.24446778431292415 1108 2 0.21641572002106102 1108 3 0.2578995091194898 1108 4 0.16423422542141708 1108 5 0.15886688057174325 1108 6 0.13373689320540194 1108 7 0.1475380983232455 1108 8 0.16149808032835722 1108 9 0.2142775270203785 1108 10-11 0.19193022146571792 1108 12-13 0.23940788902624632 1108 14-15 0.29747916211159264 1108 16-17 0.32610608937330454 1108 18-19 0.3241993373069292 1108 20-21 0.3156767890596228 1108 22-23 0.34968560371432744 1108 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-0.6564657492767445 2316 78-79 -0.6110703922689709 2316 80-81 -0.5864171313533824 2316 82-83 -0.4841469523080342 2316 84-85 -0.4741699430116917 2316 86-87 -0.48979898454110327 2316 88-89 -0.3984154841272094 2316 90-91 -0.35874411334222245 2316 92-93 -0.391811501490853 2316 94-95 -0.3028992604150922 2316 96-97 -0.3134527008620047 2316 98-99 -0.4613323067331976 2316 100 -0.5278915180220594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 9.0 11 33.0 12 99.0 13 254.0 14 696.0 15 1913.0 16 4187.0 17 8609.0 18 15373.0 19 24109.0 20 35502.0 21 49659.0 22 66495.0 23 78554.0 24 86766.0 25 98440.0 26 116233.0 27 141197.0 28 172530.0 29 209784.0 30 254993.0 31 311593.0 32 383070.0 33 482921.0 34 631788.0 35 864732.0 36 1268972.0 37 1954550.0 38 1984201.0 39 332074.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10605271003966 20.198719121148574 13.442640646544415 24.252587522267348 2 15.064365048790332 21.291567048370826 40.02470227326096 23.619365629577878 3 17.461931223208527 26.30193394991934 30.811612990376027 25.424521836496105 4 11.787942862243138 17.30095241773803 38.52583251081367 32.385272209205155 5 12.281186539072436 38.494862995644425 36.00667528184181 13.217275183441327 6 31.178582549988626 38.25686538169845 17.6402647826377 12.924287285675229 7 26.955502339399835 32.80988957531698 22.66124738761799 17.573360697665194 8 25.36990660078857 37.1410675716727 20.193134996153166 17.29589083138556 9 26.20025289744834 15.068213726216225 20.156676159629168 38.57485721670626 10-11 24.352024870085536 26.574052809263232 29.413135296856375 19.66078702379486 12-13 24.888562725353832 24.19200407542033 29.305106384755593 21.614326814470246 14-15 22.126275395693668 25.87922182855258 26.518522075541423 25.475980700212332 16-17 21.91168843858515 28.772609139361993 27.27351922559783 22.042183196455028 18-19 21.71935928193377 28.031204003932025 28.97099245470708 21.278444259427125 20-21 22.493210857429286 27.26991100347147 28.92754905572105 21.30932908337819 22-23 22.17925492982087 27.193567182509053 28.712887927657526 21.914289960012546 24-25 21.98491550857963 27.306421347948067 28.8489019377569 21.859761205715404 26-27 22.036883804319363 27.446653889534407 28.739653464088157 21.776808842058077 28-29 21.96755918346298 27.499959678511683 28.863948199753853 21.668532938271483 30-31 22.22608707997771 27.4247655909255 28.833020815738774 21.516126513358014 32-33 21.991828683165682 27.51347018728344 28.75463364733931 21.740067482211565 34-35 22.164363572132196 27.59151376616088 28.785485451711224 21.458637209995704 36-37 22.215800938152384 27.538292647840844 28.639563164313213 21.60634324969356 38-39 22.099627778248703 27.577402832543225 28.71861597313189 21.60435341607619 40-41 22.204335110642116 27.504232739827593 28.74547625604688 21.545955893483406 42-43 22.026803870143606 27.489355100646577 28.65991819823138 21.823922830978436 44-45 22.062394031379604 27.697458277294345 28.531943709176538 21.70820398214951 46-47 22.055806434909268 27.598021120079107 28.385456685797898 21.960715759213727 48-49 22.06012567886218 27.627592890603914 28.43200943518009 21.88027199535382 50-51 22.10260296042274 27.805640721973663 28.24878333356997 21.84297298403363 52-53 22.127847885471887 27.830592422397636 28.146648609097006 21.894911083033467 54-55 22.0126699113173 27.829881383544254 28.256448392114898 21.901000313023548 56-57 22.112480993025457 27.83629523994004 28.07633993330967 21.97488383372484 58-59 22.076479936494476 27.820221277587276 28.22771123818758 21.875587547730667 60-61 22.170909842767177 27.88574454334508 28.18253867294115 21.76080694094659 62-63 22.01718068345312 27.927738165878857 28.21942839531327 21.835652755354758 64-65 21.967163022347385 27.751738226193957 28.241162089892725 22.039936661565935 66-67 21.96233908706942 27.80970701798784 28.111769810466637 22.116184084476103 68-69 21.944602658380415 27.827639745797665 28.15564764241747 22.07210995340445 70-71 21.92129269196983 27.68012530047178 28.16104196575085 22.237540041807538 72-73 21.87519721482169 27.86421170002687 28.010557168769534 22.250033916381906 74-75 21.898993946096656 27.94422738064678 27.900679489084006 22.256099184172555 76-77 21.7949594590969 27.829054969829603 28.035692530742217 22.34029304033128 78-79 21.783175545324152 27.765180790575293 28.05173194451071 22.399911719589845 80-81 21.78786647363436 27.966006896024332 27.812484095583876 22.43364253475743 82-83 21.838043944123132 27.978115118797596 27.860097965669727 22.323742971409537 84-85 21.824199936853606 27.925897789709143 27.8064803151974 22.443421958239853 86-87 21.6902256552912 27.92320952017096 27.910935715018848 22.47562910951899 88-89 21.60554949593676 27.80826787230951 27.937904792353052 22.648277839400677 90-91 21.762065895203243 27.918643681995437 27.813618780542747 22.50567164225857 92-93 21.691413306389084 27.845355369919357 27.802677167015254 22.66055415667631 94-95 21.49654079962139 27.815545584158492 27.852925269763958 22.834988346456168 96-97 21.48920392775805 27.7670482048729 27.901046341313673 22.84270152605537 98-99 21.47670469367256 27.738383721478378 27.782746734544478 23.002164850304588 100 20.41149560778659 28.31841941918479 28.02529196590638 23.24479300712224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2145.0 1 1597.0 2 1745.5 3 2578.5 4 2138.0 5 1846.0 6 2924.0 7 5637.5 8 8886.5 9 10436.0 10 9165.5 11 7451.5 12 7525.0 13 7993.5 14 8449.5 15 8990.5 16 9669.0 17 10469.5 18 11485.5 19 12859.5 20 14487.0 21 17075.5 22 21063.5 23 26604.0 24 33398.0 25 41718.0 26 52482.5 27 65582.0 28 81068.5 29 98826.0 30 118973.0 31 138804.5 32 159281.0 33 182163.5 34 205674.5 35 227617.5 36 252502.5 37 277129.0 38 293770.0 39 307491.0 40 319199.5 41 329354.0 42 339201.5 43 349235.0 44 359148.0 45 369026.5 46 376487.5 47 379740.5 48 382744.5 49 383047.5 50 377363.0 51 361153.5 52 339216.0 53 320405.0 54 298440.5 55 270366.5 56 238438.5 57 206883.0 58 178303.0 59 149844.0 60 122045.5 61 97958.0 62 77266.0 63 58119.0 64 41817.5 65 29951.5 66 21351.5 67 15459.5 68 11246.5 69 8024.0 70 5806.0 71 4125.0 72 3030.5 73 2256.0 74 1600.0 75 1045.5 76 664.0 77 431.0 78 277.0 79 185.0 80 127.5 81 90.5 82 65.0 83 52.0 84 37.5 85 29.0 86 22.0 87 13.5 88 11.5 89 11.0 90 8.0 91 5.5 92 4.0 93 2.5 94 1.5 95 1.0 96 2.5 97 3.5 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003465777534662995 2 0.06443005706006026 3 7.620534940674658E-4 4 7.829316719871224E-4 5 0.0016598151446126995 6 5.323935369512432E-4 7 0.0 8 7.307362271879808E-4 9 3.131726687948489E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.098580492683242E-4 18-19 0.0010073720846234307 20-21 0.0031160680545087465 22-23 5.219544479914148E-6 24-25 5.2195444799141486E-5 26-27 2.766358574354499E-4 28-29 0.00125791021965931 30-31 0.0019416705465280632 32-33 0.0027454803964348426 34-35 0.004008610160574066 36-37 0.0013153252089383655 38-39 0.0015032288102152748 40-41 0.0049898845227979265 42-43 0.006409600621334575 44-45 0.005042079967597068 46-47 0.00655574786677217 48-49 0.00793892715394942 50-51 0.007808438541951567 52-53 0.004039927427453551 54-55 0.0023853318273207662 56-57 0.003450118901223252 58-59 0.0038729020040962985 60-61 0.007166434570922127 62-63 0.012939250765707174 64-65 0.003778950203457844 66-67 0.009478692775524095 68-69 0.011529973756130355 70-71 0.005068177689996639 72-73 0.007051604592364015 74-75 0.002828993108113469 76-77 0.0030168967093903783 78-79 0.008466101146420749 80-81 0.007020287325484531 82-83 0.016024001553336435 84-85 0.017078349538279095 86-87 0.02164023141372406 88-89 0.019432364098720376 90-91 0.022209161762034704 92-93 0.03197492948395408 94-95 0.034689092613509435 96-97 0.020961690631335222 98-99 0.01650941918996845 100 0.017067910449319264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9579380.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.49021958954162 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37639391956984 43.175737417044026 2 17.59822793779332 21.290413446878485 3 5.65826507959902 10.268090914823397 4 2.25201159445821 5.448987034678835 5 1.1002841702271757 3.327821553396923 6 0.5844600649184971 2.121247060094258 7 0.35220708395899125 1.4913558694770048 8 0.22231387167187525 1.075825193218633 9 0.16211113393679139 0.8825524280771494 >10 0.6504837671319921 6.552135219569105 >50 0.02784089086169201 1.150519079197181 >100 0.013996950579060983 1.5090236559010883 >500 0.0010526924929825732 0.42518434785785775 >1k 2.806742410867056E-4 0.35646823204506395 >5k 0.0 0.0 >10k+ 7.016855938082082E-5 0.9246385477410736 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25624 0.2674912155066403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23752 0.24794924097384174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15977 0.1667853243111767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0439088959828298E-5 0.0 5 1.0439088959828298E-5 0.0 0.0 1.0439088959828298E-5 0.0 6 2.0878177919656597E-5 0.0 0.0 1.0439088959828298E-5 0.0 7 2.0878177919656597E-5 0.0 0.0 1.0439088959828298E-5 0.0 8 2.0878177919656597E-5 0.0 0.0 1.0439088959828298E-5 0.0 9 2.0878177919656597E-5 0.0 0.0 2.0878177919656597E-5 0.0 10-11 8.351271167862639E-5 0.0 0.0 2.0878177919656597E-5 0.0 12-13 9.917134511836884E-5 1.5658633439742447E-5 0.0 2.0878177919656597E-5 0.0 14-15 1.2526906751793957E-4 6.263453375896979E-5 0.0 2.6097722399570743E-5 0.0 16-17 1.513667899175103E-4 8.351271167862639E-5 0.0 3.131726687948489E-5 0.0 18-19 1.5658633439742447E-4 8.351271167862639E-5 0.0 3.131726687948489E-5 3.131726687948489E-5 20-21 1.5658633439742447E-4 8.351271167862639E-5 0.0 3.653681135939904E-5 3.131726687948489E-5 22-23 1.722449678371669E-4 8.351271167862639E-5 0.0 5.2195444799141486E-5 4.1756355839313186E-5 24-25 1.9834269023673767E-4 8.351271167862639E-5 0.0 1.4614724543759617E-4 6.263453375896979E-5 26-27 2.1922086815639425E-4 8.351271167862639E-5 0.0 2.5053813503587915E-4 7.307362271879809E-5 28-29 2.5053813503587915E-4 8.351271167862639E-5 0.0 9.342984619046327E-4 8.351271167862639E-5 30-31 2.5053813503587915E-4 8.351271167862639E-5 0.0 0.004029488338493723 8.351271167862639E-5 32-33 2.557576795157933E-4 8.351271167862639E-5 0.0 0.010882750240620999 1.0439088959828297E-4 34-35 2.975140353551065E-4 8.351271167862639E-5 0.0 0.021556718702045435 1.0439088959828297E-4 36-37 3.653681135939904E-4 8.873225615854054E-5 0.0 0.04052454334205345 1.0439088959828297E-4 38-39 3.653681135939904E-4 9.395180063845469E-5 0.0 0.07058911954635895 1.0961043407819712E-4 40-41 3.653681135939904E-4 9.917134511836884E-5 0.0 0.10416122964116675 1.1482997855811127E-4 42-43 3.653681135939904E-4 1.0439088959828297E-4 0.0 0.13799953650445018 1.1482997855811127E-4 44-45 3.705876580739046E-4 1.0439088959828297E-4 0.0 0.18037179859239325 1.3570815647776787E-4 46-47 3.8102674703373285E-4 1.0439088959828297E-4 0.0 0.23287519651584965 1.3570815647776787E-4 48-49 3.86246291513647E-4 1.0439088959828297E-4 0.0 0.29005530629330917 1.4614724543759617E-4 50-51 3.86246291513647E-4 1.0439088959828297E-4 0.0 0.35074816950575094 1.4614724543759617E-4 52-53 3.9668538047347534E-4 1.0439088959828297E-4 0.0 0.4120412803333827 1.513667899175103E-4 54-55 3.9668538047347534E-4 1.0439088959828297E-4 0.0 0.48338201428484934 1.5658633439742447E-4 56-57 3.9668538047347534E-4 1.0439088959828297E-4 0.0 0.5712895824155635 1.6702542335725277E-4 58-59 3.9668538047347534E-4 1.0439088959828297E-4 0.0 0.6700068271641797 1.8268405679699524E-4 60-61 4.175635583931319E-4 1.0439088959828297E-4 0.0 0.7761880205190732 2.0878177919656595E-4 62-63 4.175635583931319E-4 1.0439088959828297E-4 0.0 0.8840707853744187 2.1922086815639425E-4 64-65 4.175635583931319E-4 1.0439088959828297E-4 0.0 0.9981021736271032 2.2965995711622255E-4 66-67 4.175635583931319E-4 1.0439088959828297E-4 0.0 1.1329230075432857 2.2965995711622255E-4 68-69 4.488808252726168E-4 1.0439088959828297E-4 0.0 1.287640745016901 2.2965995711622255E-4 70-71 4.541003697525309E-4 1.0439088959828297E-4 0.0 1.4614672348314817 2.2965995711622255E-4 72-73 4.697590031922734E-4 1.0439088959828297E-4 0.0 1.636306316275166 2.2965995711622255E-4 74-75 4.697590031922734E-4 1.0439088959828297E-4 0.0 1.8129826773757802 2.2965995711622255E-4 76-77 4.697590031922734E-4 1.1482997855811127E-4 0.0 2.012953865490251 2.5053813503587915E-4 78-79 4.697590031922734E-4 1.1482997855811127E-4 0.0 2.241940501368565 2.5053813503587915E-4 80-81 4.697590031922734E-4 1.1482997855811127E-4 0.0 2.49271351590604 2.5053813503587915E-4 82-83 4.697590031922734E-4 1.1482997855811127E-4 0.0 2.75355503174527 2.5053813503587915E-4 84-85 4.697590031922734E-4 1.1482997855811127E-4 0.0 3.0157431900603173 2.5053813503587915E-4 86-87 4.697590031922734E-4 1.1482997855811127E-4 0.0 3.2919145080370544 2.661967684756216E-4 88 4.697590031922734E-4 1.1482997855811127E-4 0.0 3.518192200330293 2.81855401915364E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15220 0.0 44.832367 1 GTATCAA 23695 0.0 33.361835 1 TACCTGG 8400 0.0 27.934866 2 TCAACGC 28550 0.0 27.109987 4 ATCAACG 28775 0.0 26.783688 3 CAACGCA 29125 0.0 26.639032 5 GTACCTG 9040 0.0 26.374151 1 AACGCAG 30415 0.0 25.663157 6 GTACATG 28475 0.0 25.251238 1 TATCAAC 30940 0.0 25.138567 2 TACATGG 29070 0.0 24.021866 2 ACATGGG 28620 0.0 23.52981 3 ACGCAGA 34215 0.0 22.565605 7 CATGGGG 15960 0.0 22.142508 4 CGCAGAG 34950 0.0 21.970041 8 ACCTGGG 11000 0.0 20.720055 3 TATACCG 715 0.0 19.06028 5 GCAGAGT 39220 0.0 19.014956 9 GAGTACT 23985 0.0 18.985521 12-13 AGAGTAC 35630 0.0 18.220629 10-11 >>END_MODULE