FastQCFastQC Report
Mon 6 Jun 2016
ERR1211501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286518
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210100.17100044129671238No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186810.15204470461037048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA181650.042.798211
GTATCAA287850.035.41651
TCAACGC332400.029.4897884
GTACATG440850.029.3307821
CAACGCA335650.029.1881075
ATCAACG336700.029.1140043
TACCTGG113200.028.5796892
TACATGG446700.028.2541392
AACGCAG353350.027.9592326
TATCAAC361950.027.3865622
ACATGGG451700.026.9035243
GTACCTG125050.026.5848961
CATGGGG281900.025.6377334
ACGCAGA397750.024.5794627
ATGGGGG184100.023.9916765
CGCAGAG412400.023.4330988
ACCTGGG143650.021.55813
GCAGAGT469300.019.8429419
TGGGGGG180700.019.0822246
GAGTACT225050.018.5712112-13
Produced by FastQC (version 0.11.5)