FastQCFastQC Report
Mon 6 Jun 2016
ERR1211501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12286518
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227150.18487744045953458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207990.16928311178154787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125090.10181078154119824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196850.040.6355441
GTATCAA303200.032.8964231
TACCTGG107450.028.1846772
GTACATG469800.027.3667141
TCAACGC362700.027.0266824
TACATGG475600.026.7262382
ATCAACG367200.026.6827853
CAACGCA367850.026.6736345
TATCAAC385100.025.7535252
ACATGGG472450.025.6620663
GTACCTG122700.025.6017171
AACGCAG384000.025.6004526
CATGGGG290600.025.0970154
ATGGGGG185050.022.803935
ACGCAGA441000.022.1421897
CGCAGAG450800.021.4627848
ACCTGGG142450.020.7828853
TGGGGGG157150.019.5560236
GCAGAGT511400.018.4690869
GAGTACT239550.018.19435512-13
Produced by FastQC (version 0.11.5)