FastQCFastQC Report
Mon 6 Jun 2016
ERR1211500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8054293
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182190.22620234947996054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161470.20047693819929321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96480.11978705021036606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116350.045.9806561
GTATCAA193750.035.7790951
TCAACGC225900.029.6214664
CAACGCA227900.029.317185
ATCAACG231500.028.9462343
TACCTGG69650.028.7618582
AACGCAG238450.028.1321816
GTACATG240200.027.5465091
TATCAAC245950.027.5324062
GTACCTG77800.026.7550581
TACATGG248350.025.865152
ACATGGG251000.024.4882413
ACGCAGA270750.024.4821727
CGCAGAG277650.023.6888168
CATGGGG155600.023.3141984
ACCTGGG91050.021.5218523
GCAGAGT311900.020.1703419
GAGTACT179950.019.3872212-13
ATGGGGG96500.018.6969555
AGAGTAC278200.018.36595210-11
Produced by FastQC (version 0.11.5)