FastQCFastQC Report
Mon 6 Jun 2016
ERR1211500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8054293
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200020.24833961217949235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182290.22632650687031125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120130.14915027302830924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124150.045.341931
GTATCAA206100.035.6871071
TACCTGG70950.031.1517052
TCAACGC248500.028.8368684
CAACGCA250900.028.5235715
ATCAACG254100.028.1273753
GTACCTG81250.027.4351631
AACGCAG263150.027.3027296
TATCAAC269100.026.7370872
GTACATG258850.025.5622161
TACATGG267050.024.318542
ACATGGG267200.023.7059883
ACGCAGA300550.023.6549177
ACCTGGG91350.023.4563773
CGCAGAG308450.022.8510278
CATGGGG165950.021.63324
GCAGAGT347850.019.6278539
AGAGTAC314450.018.76895110-11
GAGTACT195350.018.73893212-13
ATGGGGG92700.018.0964375
Produced by FastQC (version 0.11.5)