FastQCFastQC Report
Mon 6 Jun 2016
ERR1211499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5921268
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200570.33872812377348904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184930.3123148622896312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105750.17859350395894932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111100.047.006811
GTATCAA183850.036.3976821
TCAACGC217650.029.8578974
ATCAACG219400.029.6212443
CAACGCA219050.029.5800155
AACGCAG227700.028.5939966
TATCAAC229350.028.4194852
TACCTGG54300.027.973992
GTACCTG57300.027.367361
ACGCAGA254550.025.395287
GTACATG203100.025.06451
CGCAGAG260650.024.603528
TACATGG207600.023.8535352
CATGGGG120300.023.4358844
ACATGGG205550.023.181323
ACCTGGG63400.022.3097633
GCAGAGT288800.021.623019
GAGTACT167300.021.51436412-13
TATAACG5050.021.4184552
ATGGGGG76100.020.1284485
Produced by FastQC (version 0.11.5)