FastQCFastQC Report
Mon 6 Jun 2016
ERR1211499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5921268
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221530.374125947347764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205350.3468007190351796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129150.21811206653709983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117350.046.2074241
GTATCAA190550.035.4660951
TACCTGG52350.029.824942
GTACCTG55800.028.1499631
TCAACGC236350.028.0940654
CAACGCA238900.027.8129225
ATCAACG238200.027.7776993
TATCAAC247150.026.9629522
AACGCAG247950.026.8354456
GTACATG211100.024.0825791
ACGCAGA276800.023.902667
CGCAGAG282850.023.3249448
ACCTGGG65550.022.5825613
TACATGG219500.022.5178242
ACATGGG215050.022.0489063
CATGGGG123300.021.914564
GAGTACT185000.020.67597612-13
GCAGAGT313800.020.545059
ATGGGGG75950.020.2317435
AGAGTAC286800.019.39896610-11
Produced by FastQC (version 0.11.5)