##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5921268 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1243688007366 33.0 31.0 34.0 30.0 34.0 2 32.34241280752703 34.0 31.0 34.0 31.0 34.0 3 32.353792295839334 34.0 31.0 34.0 30.0 34.0 4 35.98950967934571 37.0 35.0 37.0 35.0 37.0 5 35.91298654274726 37.0 35.0 37.0 35.0 37.0 6 35.93344364754306 37.0 35.0 37.0 35.0 37.0 7 35.86399450252885 37.0 35.0 37.0 35.0 37.0 8 35.85742074163845 37.0 35.0 37.0 35.0 37.0 9 37.516103138719615 39.0 37.0 39.0 35.0 39.0 10-11 37.47906377485363 39.0 37.0 39.0 35.0 39.0 12-13 37.393656308074554 39.0 37.0 39.0 34.0 39.0 14-15 38.62688363370819 40.0 38.0 41.0 34.0 41.0 16-17 38.55373975641704 40.0 38.0 41.0 34.0 41.0 18-19 38.54873668950637 40.0 38.0 41.0 34.0 41.0 20-21 38.49535141797331 40.0 38.0 41.0 34.0 41.0 22-23 38.39005175918402 40.0 38.0 41.0 34.0 41.0 24-25 38.28101776511382 40.0 38.0 41.0 34.0 41.0 26-27 38.10675880571526 40.0 38.0 41.0 33.0 41.0 28-29 38.14103626115217 40.0 38.0 41.0 33.0 41.0 30-31 38.06230143611132 40.0 37.5 41.0 33.0 41.0 32-33 37.87858478960925 40.0 37.0 41.0 33.0 41.0 34-35 37.76335119437256 40.0 37.0 41.0 33.0 41.0 36-37 37.71038652531856 40.0 37.0 41.0 33.0 41.0 38-39 37.61678039230786 40.0 37.0 41.0 32.5 41.0 40-41 37.45684176092013 40.0 37.0 41.0 32.0 41.0 42-43 37.30674460267632 40.0 36.0 41.0 31.0 41.0 44-45 37.12859357151205 40.0 36.0 41.0 31.0 41.0 46-47 36.87068166818324 39.0 36.0 41.0 30.0 41.0 48-49 36.65078611540636 39.0 35.5 41.0 30.0 41.0 50-51 36.44898373118731 39.0 35.0 40.5 29.5 41.0 52-53 36.254082824827385 39.0 35.0 40.0 29.0 41.0 54-55 36.0630212988164 39.0 35.0 40.0 28.0 41.0 56-57 35.86559694646485 38.0 35.0 40.0 28.0 41.0 58-59 35.6150269671969 38.0 34.0 40.0 27.5 41.0 60-61 35.302994054651805 38.0 34.0 40.0 26.5 41.0 62-63 34.984355462377316 37.5 34.0 40.0 26.0 41.0 64-65 34.672271293918804 37.0 33.0 40.0 26.0 41.0 66-67 34.33419649304845 36.5 33.0 39.0 26.0 41.0 68-69 33.94174676775312 36.0 32.5 39.0 25.5 40.0 70-71 33.550162735414105 35.5 32.0 39.0 25.0 40.0 72-73 33.139105340275094 35.0 32.0 38.0 24.0 40.0 74-75 32.730360287695135 35.0 31.0 37.0 23.0 39.0 76-77 31.18214899241176 33.5 29.5 35.5 21.5 38.5 78-79 32.01936004247739 34.0 31.0 36.0 22.5 39.0 80-81 31.986910911649332 34.5 31.0 36.0 23.0 37.5 82-83 31.695830099228747 34.0 31.0 35.5 22.0 37.0 84-85 31.393964856851607 34.0 31.0 35.0 21.0 37.0 86-87 31.088673405763764 34.0 30.0 35.0 20.0 36.0 88-89 30.811370216649543 34.0 30.0 35.0 20.0 36.0 90-91 30.52381440934611 34.0 29.5 35.0 19.5 35.5 92-93 30.312993517604674 34.0 29.0 35.0 19.0 35.0 94-95 30.08958866580604 34.0 29.0 35.0 18.0 35.0 96-97 29.88290421916387 34.0 29.0 35.0 18.0 35.0 98-99 29.719460679705765 34.0 29.0 35.0 18.0 35.0 100 27.828420027602196 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2807127135097076 1101 2 0.21658000603054006 1101 3 0.28108311130781516 1101 4 0.18165188625410877 1101 5 0.17261972050483365 1101 6 0.18253030203127452 1101 7 0.2177144021408921 1101 8 0.16606460268477008 1101 9 0.1824540664285621 1101 10-11 0.19516840937080815 1101 12-13 0.21847068134486136 1101 14-15 0.27763974332146546 1101 16-17 0.2989854345500902 1101 18-19 0.3487710536347848 1101 20-21 0.3226115500807438 1101 22-23 0.3223564031141706 1101 24-25 0.2513818394174123 1101 26-27 0.25908762563857124 1101 28-29 0.3324032385977702 1101 30-31 0.43295727684208174 1101 32-33 0.42546361843501046 1101 34-35 0.3654340599919834 1101 36-37 0.40202758510212533 1101 38-39 0.41441867747865047 1101 40-41 0.43481128268778235 1101 42-43 0.49001813834046004 1101 44-45 0.5418199152764416 1101 46-47 0.5103165504023863 1101 48-49 0.5384316903363242 1101 50-51 0.45481416760882354 1101 52-53 0.44022592347874223 1101 54-55 0.31510434091654815 1101 56-57 0.41055753789395055 1101 58-59 0.3816418800663328 1101 60-61 0.4541897074008787 1101 62-63 0.4128400513856292 1101 64-65 0.4179018710213427 1101 66-67 0.2932540062933384 1101 68-69 0.33857735251248755 1101 70-71 0.3559634550761359 1101 72-73 0.14033091079821247 1101 74-75 0.18807146504019556 1101 76-77 0.2451651056659898 1101 78-79 0.33101580134465536 1101 80-81 0.33380058645340327 1101 82-83 0.32827545962224747 1101 84-85 0.27240600885357225 1101 86-87 0.17098320336156192 1101 88-89 0.2316660600622491 1101 90-91 0.18871366111985566 1101 92-93 0.14126964577561907 1101 94-95 0.19391444244729428 1101 96-97 0.05563171049660198 1101 98-99 0.09216787986798636 1101 100 0.16818423588460263 1102 1 0.31092966920165566 1102 2 0.2047386147694894 1102 3 0.3038732234656152 1102 4 0.20223792985009226 1102 5 0.16752913659294677 1102 6 0.17315399650025398 1102 7 0.24712256566835578 1102 8 0.22496606058612656 1102 9 0.29381587333642756 1102 10-11 0.25840026662057625 1102 12-13 0.233333112377764 1102 14-15 0.30606855825120505 1102 16-17 0.3732436399441923 1102 18-19 0.29863903082198107 1102 20-21 0.28645520730582774 1102 22-23 0.30743857317067835 1102 24-25 0.290587529321499 1102 26-27 0.2970297266909441 1102 28-29 0.3555013421718414 1102 30-31 0.3431333284640914 1102 32-33 0.433101422396156 1102 34-35 0.41335411765695085 1102 36-37 0.4278845077566231 1102 38-39 0.38521169882224626 1102 40-41 0.416465892303286 1102 42-43 0.3770327172066885 1102 44-45 0.3952607771051717 1102 46-47 0.39094753159040607 1102 48-49 0.3558656796053654 1102 50-51 0.33544519792024374 1102 52-53 0.3594571364260375 1102 54-55 0.36231982170307475 1102 56-57 0.3829142306386757 1102 58-59 0.3901286468495968 1102 60-61 0.4524214984568644 1102 62-63 0.44050439573553035 1102 64-65 0.3929394731393714 1102 66-67 0.4170234375748265 1102 68-69 0.46304894134513574 1102 70-71 0.42972948087814444 1102 72-73 0.4186634991343041 1102 74-75 0.4010657848772752 1102 76-77 0.4186026384021275 1102 78-79 0.3635763360449715 1102 80-81 0.3779639323579822 1102 82-83 0.37432725626504393 1102 84-85 0.23724367801029445 1102 86-87 0.19402650978665648 1102 88-89 0.24124864701673587 1102 90-91 0.2751477881063451 1102 92-93 0.11685329129018385 1102 94-95 0.18518468678240652 1102 96-97 0.21793514291779914 1102 98-99 0.23472814021412702 1102 100 0.33955525785044927 1103 1 0.23891279367227014 1103 2 0.2064616622617379 1103 3 0.30772505931749095 1103 4 0.1623006740425481 1103 5 0.19611609894265314 1103 6 0.20875217347396813 1103 7 0.2037418455196871 1103 8 0.18453864417398336 1103 9 0.2190542084299807 1103 10-11 0.23085338891444707 1103 12-13 0.2731160331217808 1103 14-15 0.35498313566488804 1103 16-17 0.33871694792992457 1103 18-19 0.30649885512465147 1103 20-21 0.2835348583562478 1103 22-23 0.3477298777033724 1103 24-25 0.2755575636628791 1103 26-27 0.30651850515553036 1103 28-29 0.34391134927314937 1103 30-31 0.3946747706870539 1103 32-33 0.40685725124419747 1103 34-35 0.45319914549943974 1103 36-37 0.46436198923414196 1103 38-39 0.4285830801869537 1103 40-41 0.45709572476526006 1103 42-43 0.47936739884838175 1103 44-45 0.4129997793059701 1103 46-47 0.5455300549446633 1103 48-49 0.4878435149522531 1103 50-51 0.4232047880143526 1103 52-53 0.41056989139696043 1103 54-55 0.4657633075676415 1103 56-57 0.497373499741677 1103 58-59 0.5098583979864344 1103 60-61 0.4634895119236617 1103 62-63 0.5548276844912579 1103 64-65 0.5685909388166692 1103 66-67 0.4734165753646238 1103 68-69 0.5039565458320183 1103 70-71 0.4977262550397583 1103 72-73 0.45104595373788925 1103 74-75 0.42954362335702 1103 76-77 0.48232326589379326 1103 78-79 0.4959298272650585 1103 80-81 0.45216898899979796 1103 82-83 0.42629525731041085 1103 84-85 0.3888687483859812 1103 86-87 0.44392980972787655 1103 88-89 0.4848036903355428 1103 90-91 0.5054124145671821 1103 92-93 0.45897990482518836 1103 94-95 0.5197847904871509 1103 96-97 0.5224237068653714 1103 98-99 0.5221047755954302 1103 100 0.5413859177884746 1104 1 0.24681406907181724 1104 2 0.21261984968071346 1104 3 0.240006268932774 1104 4 0.06857807634881397 1104 5 0.10422700393576179 1104 6 0.14534731216858887 1104 7 0.22897436688766248 1104 8 0.2095406872678538 1104 9 0.2812228418926068 1104 10-11 0.29415936063534076 1104 12-13 0.2790179575097369 1104 14-15 0.35987477294317927 1104 16-17 0.257411535310915 1104 18-19 0.2734780407277526 1104 20-21 0.18059761922284423 1104 22-23 0.2633481083043776 1104 24-25 0.30113296947990165 1104 26-27 0.3126546290813863 1104 28-29 0.3545138300452564 1104 30-31 0.32518890817637924 1104 32-33 0.30193448979564863 1104 34-35 0.3735510157202029 1104 36-37 0.27045298263295336 1104 38-39 0.36586809317392266 1104 40-41 0.4815826729246524 1104 42-43 0.5597253056792653 1104 44-45 0.3757511050602176 1104 46-47 0.4488989654805877 1104 48-49 0.4272674383474211 1104 50-51 0.42487947676544735 1104 52-53 0.4209330108781444 1104 54-55 0.5089265344901222 1104 56-57 0.5148558290204193 1104 58-59 0.5276456548234449 1104 60-61 0.4850011056050576 1104 62-63 0.5041991942459205 1104 64-65 0.593177544821458 1104 66-67 0.5272640308220531 1104 68-69 0.5174573389173602 1104 70-71 0.498303122953331 1104 72-73 0.5232435612005446 1104 74-75 0.49570711753825236 1104 76-77 0.44010377547933643 1104 78-79 0.6406829797516878 1104 80-81 0.713084007914869 1104 82-83 0.5044559379281246 1104 84-85 0.6431772036063137 1104 86-87 0.6553488990333101 1104 88-89 0.6837716470371262 1104 90-91 0.45707663096169426 1104 92-93 0.5960557299686222 1104 94-95 0.5991517847882868 1104 96-97 0.5002686226442883 1104 98-99 0.6195239086917361 1104 100 0.7730325726988276 1105 1 0.2497524260936288 1105 2 0.16701982694997497 1105 3 0.2343816997258159 1105 4 0.13914647089726628 1105 5 0.089572535776 1105 6 0.11560640905073427 1105 7 0.13901161912829707 1105 8 0.11847112949607919 1105 9 0.17043842842819856 1105 10-11 0.1966949451683746 1105 12-13 0.20043049356230824 1105 14-15 0.2971335720195256 1105 16-17 0.2934595015542101 1105 18-19 0.3486220065502934 1105 20-21 0.27458679039880707 1105 22-23 0.30668096824277313 1105 24-25 0.2815324729649902 1105 26-27 0.3100055027431736 1105 28-29 0.36636096405732843 1105 30-31 0.4222229502349606 1105 32-33 0.4925856403959088 1105 34-35 0.4785036706313974 1105 36-37 0.42154670738653977 1105 38-39 0.4249083965981839 1105 40-41 0.5044657108532178 1105 42-43 0.3868077702501793 1105 44-45 0.39930789150748325 1105 46-47 0.48104592390837553 1105 48-49 0.505025463766053 1105 50-51 0.5860237214677113 1105 52-53 0.5819984861659364 1105 54-55 0.5331581033350545 1105 56-57 0.5455648928987102 1105 58-59 0.5644836019085915 1105 60-61 0.611363411438326 1105 62-63 0.5985705722333847 1105 64-65 0.6194946908211705 1105 66-67 0.5740993929665166 1105 68-69 0.6265487938079133 1105 70-71 0.6711240211066212 1105 72-73 0.6326488044411249 1105 74-75 0.5703644912628363 1105 76-77 0.6516015386760969 1105 78-79 0.6760485493827559 1105 80-81 0.634501956627954 1105 82-83 0.6094457516659411 1105 84-85 0.6585557641091242 1105 86-87 0.5928728454948278 1105 88-89 0.6495824005753157 1105 90-91 0.6577768905699202 1105 92-93 0.620071473537056 1105 94-95 0.7308114553783867 1105 96-97 0.7168061566108612 1105 98-99 0.7037086474412213 1105 100 0.8226240980634287 1106 1 0.24603975320426485 1106 2 0.167846290306656 1106 3 0.22901538765467677 1106 4 0.16170322078737343 1106 5 0.15776217491787037 1106 6 0.1451774104464647 1106 7 0.15471869771570113 1106 8 0.14124019497263873 1106 9 0.17512647057213115 1106 10-11 0.19496507186663337 1106 12-13 0.22382902487993306 1106 14-15 0.23471884558245648 1106 16-17 0.28217863296971757 1106 18-19 0.2912910984594319 1106 20-21 0.2705118273224798 1106 22-23 0.27965668518192643 1106 24-25 0.2998407737207103 1106 26-27 0.30824178719191053 1106 28-29 0.34256238172697095 1106 30-31 0.39633184818169553 1106 32-33 0.4798007509959419 1106 34-35 0.4720526254046149 1106 36-37 0.4355028948540749 1106 38-39 0.39729703956672324 1106 40-41 0.4912950198087316 1106 42-43 0.4938807291528633 1106 44-45 0.5102481289447027 1106 46-47 0.575300507395788 1106 48-49 0.5456621682861638 1106 50-51 0.5627862080109693 1106 52-53 0.6480916870086375 1106 54-55 0.565576128500858 1106 56-57 0.6570163264207807 1106 58-59 0.6909253707966627 1106 60-61 0.6671119271885075 1106 62-63 0.7383055767326212 1106 64-65 0.6957124240174295 1106 66-67 0.6908626347262796 1106 68-69 0.6750397500901428 1106 70-71 0.7900485357909517 1106 72-73 0.8385553813082751 1106 74-75 0.7940596231799191 1106 76-77 0.7233132654074126 1106 78-79 0.835107478357493 1106 80-81 0.8819443731498033 1106 82-83 0.7991472345264476 1106 84-85 0.7682395635036698 1106 86-87 0.7712749355921567 1106 88-89 0.7794262061255885 1106 90-91 0.8085690485726147 1106 92-93 0.8161238998126059 1106 94-95 0.8577986188350266 1106 96-97 0.8964333361407881 1106 98-99 0.8975752206320315 1106 100 0.970432082126937 1107 1 0.16146334766848014 1107 2 0.1596134722848106 1107 3 0.20062557146204796 1107 4 0.15812242216966155 1107 5 0.11740241861645018 1107 6 0.13338168019033247 1107 7 0.11127045858235363 1107 8 0.10782976403130107 1107 9 0.10491089386670183 1107 10-11 0.11735274607050883 1107 12-13 0.17082515520741026 1107 14-15 0.1755227904683494 1107 16-17 0.1434115169774799 1107 18-19 0.12074245767237102 1107 20-21 0.17143873442793023 1107 22-23 0.19166401814630518 1107 24-25 0.2423045548634306 1107 26-27 0.2776928505625662 1107 28-29 0.21084499131459467 1107 30-31 0.14634667174208715 1107 32-33 0.09285635432818395 1107 34-35 0.15904207261448988 1107 36-37 0.2729534044763895 1107 38-39 0.22280505499349346 1107 40-41 0.2806526669508358 1107 42-43 0.2186032380142393 1107 44-45 0.25660088530556635 1107 46-47 0.18958047266651334 1107 48-49 0.2769857211639106 1107 50-51 0.22452437103007838 1107 52-53 0.2344539510766097 1107 54-55 0.27722879787755517 1107 56-57 0.28706322673191664 1107 58-59 0.4008564694792227 1107 60-61 0.3400956192092437 1107 62-63 0.33573953729941053 1107 64-65 0.41985341158579104 1107 66-67 0.4667436466548338 1107 68-69 0.37113702675870286 1107 70-71 0.4513121058894356 1107 72-73 0.3942332275683498 1107 74-75 0.43457152946110256 1107 76-77 0.367115134189838 1107 78-79 0.47929873446184956 1107 80-81 0.4492074513350097 1107 82-83 0.41798385031301777 1107 84-85 0.39176276394167786 1107 86-87 0.3741250867673145 1107 88-89 0.3606073559704903 1107 90-91 0.21421465280269913 1107 92-93 0.3733242898716078 1107 94-95 0.5696761006807876 1107 96-97 0.5807863908092976 1107 98-99 0.6095765864372815 1107 100 0.8595950695695649 1108 1 0.24468388488194392 1108 2 0.17612530002288196 1108 3 0.19023263269421165 1108 4 0.15974325682193324 1108 5 0.14036084060122533 1108 6 0.12175140904142978 1108 7 0.14508289303789468 1108 8 0.1682083724978014 1108 9 0.1224414930747102 1108 10-11 0.11340749027123564 1108 12-13 0.15184383413598823 1108 14-15 0.19004851737390283 1108 16-17 0.2849521618219839 1108 18-19 0.29489045797612334 1108 20-21 0.2913241573405685 1108 22-23 0.2769465945412364 1108 24-25 0.2971417052101941 1108 26-27 0.3330376174713052 1108 28-29 0.3380381874562701 1108 30-31 0.3326592334419942 1108 32-33 0.3794324411515717 1108 34-35 0.3462910711945213 1108 36-37 0.42597516557652426 1108 38-39 0.38142515992994674 1108 40-41 0.3565163023618467 1108 42-43 0.4175950543642841 1108 44-45 0.4110168908232126 1108 46-47 0.35974660889882415 1108 48-49 0.3736790551649918 1108 50-51 0.4593766437233313 1108 52-53 0.49757692792521624 1108 54-55 0.39508402672279885 1108 56-57 0.42323375105763716 1108 58-59 0.5487681784921747 1108 60-61 0.4804073692670059 1108 62-63 0.4434891138546533 1108 64-65 0.4513109406825606 1108 66-67 0.5365805261820498 1108 68-69 0.5380338673695562 1108 70-71 0.5750510597856007 1108 72-73 0.5150613475117396 1108 74-75 0.5406319540680684 1108 76-77 0.5038784675621812 1108 78-79 0.5419284114408001 1108 80-81 0.6367387010543233 1108 82-83 0.6093706723693266 1108 84-85 0.5866453357639827 1108 86-87 0.6437518157596784 1108 88-89 0.6461415359846576 1108 90-91 0.5991431496343829 1108 92-93 0.6665241604225791 1108 94-95 0.8200843033289402 1108 96-97 0.7928370174639845 1108 98-99 0.7978971594969089 1108 100 0.8775151590189516 1109 1 0.1455162464988078 1109 2 0.10516166932489313 1109 3 0.19383782183512466 1109 4 0.1139789939677982 1109 5 0.12298848177309196 1109 6 0.09509208183591511 1109 7 0.11985706632155768 1109 8 0.1531776478511162 1109 9 0.12272177977193621 1109 10-11 0.11194558963359924 1109 12-13 0.12751745330280073 1109 14-15 0.21147493148679786 1109 16-17 0.29258615767685114 1109 18-19 0.2301729186627668 1109 20-21 0.2783358826953588 1109 22-23 0.3263448780794036 1109 24-25 0.20005713982569517 1109 26-27 0.2940986412657409 1109 28-29 0.3469602079343872 1109 30-31 0.2903459361377898 1109 32-33 0.29690834428468094 1109 34-35 0.3123224445149759 1109 36-37 0.3078790199775625 1109 38-39 0.30753672136914645 1109 40-41 0.2894268473585342 1109 42-43 0.3958431409971439 1109 44-45 0.3152800023569 1109 46-47 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78-79 -0.17948843605226017 2316 80-81 -0.19487573801730207 2316 82-83 -0.16188384157373648 2316 84-85 -0.1929464072681384 2316 86-87 -0.13665298636376022 2316 88-89 -0.051048892488982034 2316 90-91 -0.003536580959718094 2316 92-93 0.020141547122779713 2316 94-95 0.09647561347158984 2316 96-97 0.09854494488548227 2316 98-99 -0.010732584713142757 2316 100 -0.21795093456861636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 7.0 11 23.0 12 58.0 13 180.0 14 487.0 15 1458.0 16 3351.0 17 6924.0 18 12648.0 19 19276.0 20 27147.0 21 37033.0 22 48257.0 23 55357.0 24 59201.0 25 64237.0 26 74799.0 27 89183.0 28 107874.0 29 130106.0 30 157146.0 31 190160.0 32 234880.0 33 294900.0 34 387222.0 35 528329.0 36 776222.0 37 1212015.0 38 1216960.0 39 185807.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.593571844036454 20.574706354047812 14.492315248106932 23.3394065538088 2 14.954916800612997 21.775236848825223 39.966429903749614 23.303416446812168 3 17.100282408112655 27.405087939677276 31.247692619998112 24.246937032211953 4 11.989316408921795 18.325787040868196 38.80234343202341 30.882553118186596 5 12.386762512362392 38.30031291039089 35.728276641968904 13.584647935277811 6 29.55438312647879 38.56257905874286 18.483879194018808 13.399158620759538 7 25.86856396298901 33.342672549190475 23.388351954344913 17.4004115334756 8 25.241842216193433 36.15274295009674 20.891891125066202 17.71352370864363 9 26.20415168112754 15.473841396026252 20.86934478854515 37.45266213430106 10-11 24.05184159879269 26.720872961669695 29.496444004898954 19.730841434638663 12-13 24.781136405242933 24.62195597294363 29.212383226025235 21.384524395788198 14-15 22.289963906379512 26.004717232862962 26.909599430392273 24.795719430365253 16-17 21.695234215734605 28.773562042609736 27.559726198733404 21.971477542922255 18-19 21.476717942205077 27.856788441285328 29.54405280540538 21.122440811104212 20-21 22.385118280586852 27.086397943621865 29.5023044743451 21.026179301446184 22-23 22.1502978753875 27.054965254063823 29.194954526631793 21.599782343916875 24-25 21.835331553984723 27.161420493043043 29.373742245748712 21.62950570722352 26-27 21.80128783444329 27.215990250720374 29.33776910244411 21.644952812392233 28-29 21.826758219933204 27.28020730876901 29.404002488678 21.489031982619785 30-31 22.21389899737088 27.11178890365227 29.36415994609185 21.310152152885003 32-33 21.953491254963286 27.287904117164686 29.254573663363143 21.504030964508882 34-35 22.13191321981365 27.34854870665217 29.242298931486406 21.277239142047776 36-37 22.159900020266164 27.247846720259407 29.13569040059447 21.456562858879956 38-39 22.03950787795696 27.425273047648407 29.131623993115554 21.403595081279075 40-41 22.16958801705605 27.22550895135222 29.166380254246405 21.43852277734532 42-43 22.016176009358087 27.349136536929475 28.934444904774924 21.700242548937513 44-45 21.97700593830717 27.499493959049065 28.930840394472963 21.592659708170807 46-47 22.013565325542984 27.360196567002166 28.684731923610045 21.9415061838448 48-49 21.929584179051414 27.47612108754665 28.71426502436555 21.880029709036386 50-51 22.065833993579552 27.6618366087795 28.500747622226903 21.77158177541405 52-53 22.078919948411183 27.730155316189247 28.331526643794035 21.859398091605538 54-55 21.988468436821286 27.660940291903245 28.44724157847982 21.903349692795643 56-57 22.071736771346227 27.70325141292626 28.221159006126108 22.003852809601405 58-59 22.020727926292 27.668636910679005 28.448214130699494 21.8624210323295 60-61 22.21879082785753 27.763124682318047 28.35594802374908 21.662136466075346 62-63 22.065732060970582 27.721994470333144 28.45638381948335 21.75588964921292 64-65 21.980029815773513 27.528281833540024 28.432052412529114 22.05963593815735 66-67 21.945620881473417 27.576475913560238 28.278568840778597 22.199334364187745 68-69 21.83242439215993 27.640397609438406 28.381217672360098 22.145960326041564 70-71 21.915970647183695 27.515850125596803 28.340247504995414 22.22793172222409 72-73 21.85764675749371 27.796543498625653 28.085515613690585 22.26029413019005 74-75 21.852444742588077 27.833377117549517 28.02555478232996 22.28862335753245 76-77 21.77117140822285 27.675374470937513 28.213941122635084 22.33951299820455 78-79 21.73297271853391 27.598795740011784 28.20889761028656 22.45933393116775 80-81 21.73593470087589 27.8100324323667 28.011427920952375 22.44260494580503 82-83 21.85359744863979 27.823166860153712 27.98337852535339 22.339857165853108 84-85 21.836303795372988 27.859958261791974 27.902777507987437 22.4009604348476 86-87 21.655959501654195 27.895788905489077 28.006744318845506 22.441507274011222 88-89 21.639074406829405 27.647254322749838 28.065608246639034 22.648063023781724 90-91 21.86706213027697 27.67289722310016 27.98954627375269 22.470494372870178 92-93 21.693505654504897 27.692633748863976 27.974765587720746 22.63909500891038 94-95 21.561359392707818 27.663872386465542 27.93609760844576 22.838670612380884 96-97 21.5480985662704 27.587550412804323 28.01245803331996 22.851892987605314 98-99 21.455820198968325 27.543548655587568 27.917911735411806 23.082719410032297 100 20.6829696429784 27.997079801748132 28.142599861557464 23.177350693716 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2061.0 1 1514.5 2 1746.5 3 2514.0 4 1884.5 5 1500.5 6 2265.0 7 4226.0 8 6898.5 9 8608.5 10 7496.5 11 5672.0 12 5710.0 13 6163.0 14 6592.0 15 7064.5 16 7561.0 17 8036.5 18 8568.5 19 9371.5 20 10468.0 21 11953.0 22 14017.5 23 17406.5 24 21974.5 25 27583.0 26 35119.0 27 43750.0 28 53295.5 29 64739.5 30 77308.0 31 89430.0 32 102862.5 33 118160.5 34 133304.5 35 146121.5 36 160688.0 37 174746.5 38 185249.5 39 194184.5 40 197926.5 41 200270.0 42 202938.5 43 206368.0 44 210510.0 45 215180.5 46 218785.0 47 219869.0 48 222518.5 49 223695.5 50 221774.5 51 215296.5 52 203465.5 53 192688.0 54 181700.0 55 166324.0 56 149035.5 57 131953.0 58 115027.0 59 96768.0 60 78384.5 61 63637.5 62 51226.5 63 39123.5 64 28752.5 65 21202.0 66 15670.0 67 11631.5 68 8711.5 69 6451.5 70 4759.0 71 3532.5 72 2587.5 73 1933.5 74 1498.0 75 1080.0 76 726.5 77 471.0 78 296.5 79 197.5 80 149.0 81 106.5 82 74.0 83 53.5 84 32.5 85 23.0 86 19.5 87 11.0 88 9.0 89 6.5 90 3.5 91 3.0 92 3.5 93 2.5 94 1.5 95 2.5 96 2.0 97 1.5 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003242548724361066 2 0.06919126106097545 3 8.44413730302361E-4 4 6.417544350297943E-4 5 0.0014186150669079664 6 6.417544350297943E-4 7 0.0 8 8.781902795144552E-4 9 6.755309842418888E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.586427096358415E-4 18-19 9.457433779386442E-4 20-21 0.003006112879876405 22-23 0.0 24-25 0.0 26-27 1.688827460604722E-4 28-29 8.781902795144552E-4 30-31 0.0014439474788170371 32-33 0.002811897721906862 34-35 0.004036297630845285 36-37 0.0011484026732112107 38-39 0.001595941950271462 40-41 0.0053873595993290625 42-43 0.006772198117024935 44-45 0.0051424796175413776 46-47 0.007380176002842635 48-49 0.008621464186387106 50-51 0.008393472479205468 52-53 0.00367319972681527 54-55 0.002161699149574044 56-57 0.002271472934513351 58-59 0.0029385597814522157 60-61 0.007574391160812178 62-63 0.014169262394473617 64-65 0.0040700741800573795 66-67 0.010352512333506945 68-69 0.01240443769814168 70-71 0.005691348542237913 72-73 0.0076757208084484605 74-75 0.0031158866648157116 76-77 0.0030567777036945463 78-79 0.007658832533842414 80-81 0.005590018894601629 82-83 0.016466067740896036 84-85 0.017445587668046776 86-87 0.022334743166497445 88-89 0.021625435633043463 90-91 0.024741322297859174 92-93 0.03461251880509378 94-95 0.03837015990493928 96-97 0.023525366526223775 98-99 0.018230892437227975 100 0.01894864410798498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5921268.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.719030275923956 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14703257227976 47.13149011046658 2 15.850393027240846 19.882425603216255 3 4.639774763591905 8.730065216135658 4 1.757158771171791 4.408291766749156 5 0.8236434866319354 2.5829060387317964 6 0.48549981897327277 1.8270046707084182 7 0.2769426648328884 1.2158702766244291 8 0.19256331268866156 0.9661907390841902 9 0.14181194042414302 0.8004876646454417 >10 0.6330543685231063 6.781227953600061 >50 0.03245925707175717 1.387251006615227 >100 0.017887040021000184 2.0211042210377403 >500 0.0011859849821597252 0.4826415216788296 >1k 4.883459961534593E-4 0.5665851102553123 >5k 0.0 0.0 >10k+ 1.0464557060403796E-4 1.216458100450954 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22153 0.374125947347764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20535 0.3468007190351796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12915 0.21811206653709983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.688827460604722E-5 0.0 4 0.0 0.0 0.0 1.688827460604722E-5 0.0 5 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 0.0 6 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 0.0 7 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 0.0 8 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 0.0 9 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 1.688827460604722E-5 10-11 0.0 1.688827460604722E-5 0.0 1.688827460604722E-5 1.688827460604722E-5 12-13 0.0 3.377654921209444E-5 0.0 1.688827460604722E-5 1.688827460604722E-5 14-15 0.0 6.755309842418888E-5 0.0 1.688827460604722E-5 4.222068651511805E-5 16-17 0.0 8.44413730302361E-5 0.0 1.688827460604722E-5 5.0664823818141656E-5 18-19 0.0 8.44413730302361E-5 0.0 3.377654921209444E-5 1.1821792224233053E-4 20-21 0.0 8.44413730302361E-5 0.0 3.377654921209444E-5 1.1821792224233053E-4 22-23 0.0 8.44413730302361E-5 0.0 1.0977378493930693E-4 1.2666205954535415E-4 24-25 0.0 8.44413730302361E-5 0.0 2.3643584448466108E-4 1.3510619684837776E-4 26-27 0.0 8.44413730302361E-5 0.0 4.475392770602513E-4 1.3510619684837776E-4 28-29 0.0 9.28855103332597E-5 0.0 0.0010048523390598094 1.3510619684837776E-4 30-31 0.0 1.0132964763628331E-4 0.0 0.003310101822785255 1.3510619684837776E-4 32-33 0.0 1.0132964763628331E-4 0.0 0.009305439307932017 1.3510619684837776E-4 34-35 0.0 1.0132964763628331E-4 0.0 0.019201968227075684 1.3510619684837776E-4 36-37 0.0 1.0132964763628331E-4 0.0 0.03576092147830499 1.3510619684837776E-4 38-39 0.0 1.0977378493930692E-4 0.0 0.0615915374882542 1.4355033415140137E-4 40-41 0.0 1.4355033415140137E-4 0.0 0.09239575036968434 1.5199447145442497E-4 42-43 0.0 1.5199447145442497E-4 0.0 0.12324218393762958 1.5199447145442497E-4 44-45 0.0 1.5199447145442497E-4 0.0 0.16250742239668936 1.5199447145442497E-4 46-47 0.0 1.5199447145442497E-4 0.0 0.21128075945895372 1.5199447145442497E-4 48-49 0.0 1.6043860875744858E-4 0.0 0.2649179196077597 1.5199447145442497E-4 50-51 0.0 1.688827460604722E-4 0.0 0.32227894430719906 1.5199447145442497E-4 52-53 0.0 1.688827460604722E-4 0.0 0.3833300570080598 1.6043860875744858E-4 54-55 0.0 1.773268833634958E-4 0.0 0.4528928601103682 1.688827460604722E-4 56-57 0.0 1.857710206665194E-4 0.0 0.535062760206091 1.688827460604722E-4 58-59 0.0 1.857710206665194E-4 0.0 0.6295526566269252 1.857710206665194E-4 60-61 0.0 1.857710206665194E-4 0.0 0.7343697329693573 1.857710206665194E-4 62-63 0.0 1.857710206665194E-4 0.0 0.8447177192452697 2.1954756987861384E-4 64-65 0.0 2.0265929527256662E-4 0.0 0.958553134227331 2.1954756987861384E-4 66-67 0.0 2.1954756987861384E-4 0.0 1.0923420456564372 2.1954756987861384E-4 68-69 0.0 2.1954756987861384E-4 0.0 1.2489047953917978 2.1954756987861384E-4 70-71 0.0 2.2799170718163745E-4 0.0 1.4207936543321464 2.3643584448466106E-4 72-73 0.0 2.3643584448466106E-4 0.0 1.5929780580781008 3.2087721751489716E-4 74-75 0.0 2.3643584448466106E-4 0.0 1.7719346599410803 3.2087721751489716E-4 76-77 0.0 2.533241190907083E-4 0.0 1.975657916513828 3.2087721751489716E-4 78-79 0.0 2.533241190907083E-4 0.0 2.206841507596008 3.2087721751489716E-4 80-81 0.0 2.533241190907083E-4 0.0 2.4583585813038695 3.46209629423968E-4 82-83 0.0 2.533241190907083E-4 0.0 2.7181677978432996 4.0531859054513325E-4 84-85 0.0 2.533241190907083E-4 0.0 2.984267896673483 4.0531859054513325E-4 86-87 0.0 2.533241190907083E-4 0.0 3.263355078675716 4.0531859054513325E-4 88 0.0 2.533241190907083E-4 0.0 3.49854119083953 4.0531859054513325E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11735 0.0 46.207424 1 GTATCAA 19055 0.0 35.466095 1 TACCTGG 5235 0.0 29.82494 2 GTACCTG 5580 0.0 28.149963 1 TCAACGC 23635 0.0 28.094065 4 CAACGCA 23890 0.0 27.812922 5 ATCAACG 23820 0.0 27.777699 3 TATCAAC 24715 0.0 26.962952 2 AACGCAG 24795 0.0 26.835445 6 GTACATG 21110 0.0 24.082579 1 ACGCAGA 27680 0.0 23.90266 7 CGCAGAG 28285 0.0 23.324944 8 ACCTGGG 6555 0.0 22.582561 3 TACATGG 21950 0.0 22.517824 2 ACATGGG 21505 0.0 22.048906 3 CATGGGG 12330 0.0 21.91456 4 GAGTACT 18500 0.0 20.675976 12-13 GCAGAGT 31380 0.0 20.54505 9 ATGGGGG 7595 0.0 20.231743 5 AGAGTAC 28680 0.0 19.398966 10-11 >>END_MODULE