FastQCFastQC Report
Mon 6 Jun 2016
ERR1211498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532842
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270800.3594924730931566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235100.3121000015664739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148900.19766775939280287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139150.049.4653661
GTATCAA234100.037.6479641
TCAACGC289350.029.6399124
ATCAACG290900.029.5146833
CAACGCA293550.029.1814925
TATCAAC301100.028.6233182
AACGCAG304000.028.296966
GTACATG297800.028.0456811
TACCTGG59950.027.920882
TACATGG302850.026.8588122
CATGGGG196400.026.1287524
ACATGGG307300.025.9667993
ACGCAGA340150.025.1242457
GTACCTG70550.024.557711
CGCAGAG348300.024.3210168
ATGGGGG121350.023.156045
GCAGAGT383200.021.4940859
GAGTACT216750.020.85437812-13
ACCTGGG77750.020.490033
CAGAGTA370050.019.58497210-11
Produced by FastQC (version 0.11.5)