FastQCFastQC Report
Mon 6 Jun 2016
ERR1211498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7532842
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303010.4022518990840376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271050.3598243531458645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181900.24147592635024073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147150.050.2647631
GTATCAA248650.037.6511231
TACCTGG63000.029.7794042
TCAACGC313600.029.550224
CAACGCA317750.029.2080655
ATCAACG316650.029.2062283
TATCAAC325200.028.5561872
AACGCAG329850.028.207856
GTACCTG75600.025.9985831
GTACATG298450.025.9015311
TACATGG304000.024.7010272
ACGCAGA375050.024.5575647
CGCAGAG382600.023.9255738
ACATGGG307300.023.5651383
CATGGGG194950.023.0925644
ACCTGGG86300.021.3998953
GCAGAGT423150.021.0996259
GAGTACT245650.020.73683212-13
ATGGGGG118350.020.4484425
AGAGTAC389250.019.64791510-11
Produced by FastQC (version 0.11.5)