FastQCFastQC Report
Mon 6 Jun 2016
ERR1211497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6329721
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225460.3561926347148634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203410.3213569760815682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125840.19880813072171744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111950.050.2555471
GTATCAA193850.037.4748841
TCAACGC232000.030.6071664
ATCAACG234400.030.2742143
CAACGCA236050.030.0215915
TATCAAC244200.029.2080692
AACGCAG244400.029.007336
GTACCTG58000.028.6541081
TACCTGG54850.028.545032
GTACATG210100.026.2180251
ACGCAGA274150.025.6895857
CGCAGAG277650.025.1125168
TACATGG214300.025.0118392
ACATGGG213400.024.2903983
ACCTGGG68800.022.4729963
GCAGAGT304250.022.2694389
CATGGGG129750.021.9850774
GAGTACT187500.021.46461112-13
CAGAGTA296200.020.23337210-11
TATAACG6150.019.8774742
Produced by FastQC (version 0.11.5)