FastQCFastQC Report
Mon 6 Jun 2016
ERR1211497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6329721
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245210.3873946418807401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227060.3587203922574154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152670.24119546501338687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116700.049.564721
GTATCAA196550.036.6781581
TACCTGG56800.030.550612
TCAACGC243450.029.2453214
ATCAACG244800.029.0648443
GTACCTG62100.028.7761
CAACGCA248400.028.699245
TATCAAC256500.027.9225062
AACGCAG256400.027.8952066
ACGCAGA289900.024.5900847
CGCAGAG295250.024.0490118
GTACATG214700.023.8963241
ACCTGGG71700.023.6614633
TACATGG220450.022.7825832
ACATGGG216300.021.965863
GAGTACT198550.021.19476912-13
GCAGAGT328900.021.1451239
CATGGGG127750.020.821364
AGAGTAC301650.019.75325210-11
AGTACTT213350.019.30599612-13
Produced by FastQC (version 0.11.5)