FastQCFastQC Report
Mon 6 Jun 2016
ERR1211496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084153
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237670.3354952949209313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209290.2954340483611802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128760.18175779094550895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120550.050.546881
GTATCAA204500.037.00411
TCAACGC241550.030.308444
ATCAACG242400.030.145073
CAACGCA245000.029.8782715
TATCAAC255100.028.920792
AACGCAG254550.028.8059846
TACCTGG61350.028.588542
GTACCTG67450.027.3672641
GTACATG219450.025.1489181
ACGCAGA288600.025.0671467
CGCAGAG293900.024.3925348
TACATGG225000.023.6780032
ACATGGG224200.022.9507563
ACCTGGG75200.022.6833553
CATGGGG128000.021.3656524
GCAGAGT327300.021.1454529
GAGTACT202000.020.4116212-13
CAGAGTA318900.019.39616410-11
AGAGTAC296200.019.0029610-11
Produced by FastQC (version 0.11.5)